The paper aims to establish and enhance the microorganism’s successful growth, proper activity, and biosorption potency for Ni(II) biosorption from an aqueous solution using 5,000 mg/l Ni(II) resistant Saccharomyces cerevisiae AJ208. Complex nutrients, amino acids, and vitamins were added to the specifically optimized fermentation media as essential growth factors. Amino acids such as L-cysteine (0.0002 g/ml), L-Proline (0.0002 g/ml), L-Lysine (0.0002 g/ml), L-tryptophan (0.0001 g/ml) and L-Histidine (0.0003 g/ml) led to an increase of more than 87% biosorption. Vitamins such as, Ascorbic acids (0.01 × 10−8 g/ml), folic acids (0.01 × 10−8 g/ml), pyridoxine-HCl (0.01 × 10−8 g/ml),Thiamin-HCl (0.05 × 10−8 g/ml) promotes biosorption more than 91%. The Ni(II) bio-removal increased with complex nutrients like soybean meal, malt extract, and yeast extract at the concentration of 0.03, 0.4, 0.05 in g/ml, and nickel removal reached more than 85%. The multiple linear regression (MLR) and ANN application of the experimental data have predicted Ni(II) percentage removal well. This adsorption shows that the proposed Ni(II) removal process using complex nutrients is environmentally friendly and economically feasible. Novelty statement: This study evaluates a cost-effective approach to bioremediation of Ni(II) by using complex nutrients as a growth factor. Media enriched with complex nutrients is cheap than chemical media. Ni(II) Removal significant increased up to 87%, 88.34%, 96% with soybean meal, L-proline, and L-ascorbic acids at 3,000 mg/l initial Ni(II) concentration using newly developed 5,000 mg/l Ni(II) resistant Saccharomyces cerevisiae AJ208 and their NCBI accession number: MZ027228 (AJ208 ITS 1) and MZ027229 (AJ208 ITS 2).
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