The purpose of the study was to determine the effect of three carbohydrate(CHO) foods on plasma glucose and insulin response in trained endurance athletes. Nine, male subjects consumed one of three CHO foods—applesauce(AS), graham crackers(GC) or orange juice(OJ). Test foods were prepared using a formula of 1 gram of CHO per kilogram of body weight. Two days prior to each trial, subjects controlled their dietary intake and exercise output. The composition of the test diet, as a percentage of total calories, was 45% CHO, 15% protein and 40% fat. Ten hours prior to each trial subjects refrained from consuming food and beverages with the exception of water and from engaging in exercise. On the day of the trial, the test food was consumed in six minutes(min), with an additional two min allowed for the consumption of 240 ml of water. Subjects then rested quietly for the next 90 minutes. Blood samples were collected immediately before the ingestion of the food and at 15,30,45,60,75 and 90 min postprandial. The highest mean glucose response was for GC at 30 min(120 mg/dl). At 15 min, OJ exhibited the highest mean glucose (113 mg/dl). A similar pattern was observed for plasma insulin—47 uU/L at 30 min and 40 uU/L at 15 min for GC and OJ respectively. Other than at 15 min, there was no significant difference between the glucose means for the three meals. At 15 min OJ elicited a significantly greater response than AS. There were no significant differences between the plasma insulin means at baseline or 15 min. At 30, 45 and 60 minutes, mean plasma insulin response for GC was elevated when compared to AS. Similarly, at 30 min GC produced a significantly greater insulin response than OJ. The results suggest that the ingestion of OJ elevates glucose to a greater degree than AS or GC 15 min postprandial and that a significant difference exists between mean plasma glucose levels of OJ and AS. At 30 min, plasma insulin levels in response to GC ingestion are elevated and are significantly greater than either OJ or AS. This response continues for AS at 45 and 60 min but not for OJ. These data add to the growing body of knowledge regarding endurance athletes' glycemic response to carbohydrate foods.