Color Appearance Research Articles

Willingness to pay for pesticide-free vegetables in Hokkaido, Japan: the relationship between appearance and pesticide use

Most Japanese consumers focus on the appearance of vegetables, for example, their shape, color, or size. However, giving consumers precise information about the growing process of vegetables may change their preferences. Therefore, in order to reduce food loss, it is important to change consumers’ preferences for vegetables and for producers to understand consumers’ preferences and select vegetable production methods. Japanese supermarkets sell vegetables containing legally permissible amounts of pesticides and chemical fertilizers, and chemical-free vegetables are generally hard to find. Therefore, in this study, pesticide-free vegetables were cultivated from scratch to analyze whether consumers are willing to pay a premium when presented with pesticide-free vegetables. The contingent valuation method and double-bound dichotomous choice were used for analysis. The results revealed that consumers’ willingness to pay was not related to the appearance of the pesticide-free vegetables. In other words, they cared neither about the color nor the shape of the vegetables so long as they were pesticide-free. In Japan, some farmers continue to grow organic or pesticide-free vegetables, although they are few. Therefore, if producers know the actual needs of consumers’ vegetables, they may reduce their dependence on pesticides and chemical fertilizers and expand the market for environmentally friendly and healthy vegetables. Such a change could, in turn, reduce food loss.

Distill-SODA: Distilling Self-Supervised Vision Transformer for Source-Free Open-Set Domain Adaptation in Computational Pathology.

Developing computational pathology models is essential for reducing manual tissue typing from whole slide images, transferring knowledge from the source domain to an unlabeled, shifted target domain, and identifying unseen categories. We propose a practical setting by addressing the above-mentioned challenges in one fell swoop, i.e., source-free open-set domain adaptation. Our methodology focuses on adapting a pre-trained source model to an unlabeled target dataset and encompasses both closed-set and open-set classes. Beyond addressing the semantic shift of unknown classes, our framework also deals with a covariate shift, which manifests as variations in color appearance between source and target tissue samples. Our method hinges on distilling knowledge from a self-supervised vision transformer (ViT), drawing guidance from either robustly pre-trained transformer models or histopathology datasets, including those from the target domain. In pursuit of this, we introduce a novel style-based adversarial data augmentation, serving as hard positives for self-training a ViT, resulting in highly contextualized embeddings. Following this, we cluster semantically akin target images, with the source model offering weak pseudo-labels, albeit with uncertain confidence. To enhance this process, we present the closed-set affinity score (CSAS), aiming to correct the confidence levels of these pseudo-labels and to calculate weighted class prototypes within the contextualized embedding space. Our approach establishes itself as state-of-the-art across three public histopathological datasets for colorectal cancer assessment. Notably, our self-training method seamlessly integrates with open-set detection methods, resulting in enhanced performance in both closed-set and open-set recognition tasks.

Photocolorimetry for full crown central incisor shade matching

Introduction The objective of this case series report is color matching of the central incisors all-ceramic crowns and determine the color difference between those crowns and contralateral or neighboring intact natural incisor using the ?Eab value from CIELab formula. Case Report The subject of this color assessment was all-ceramic crowns for central incisors for three young female patients. The intact natural incisors were used as the target shade for the all-ceramic crown. After tooth preparation and intraoral scan, everything was done at once, regarding the design of restoration and model. For these cases, we used Ivoclar ZirCAD PRIME multi A1 (Ivoclar, Schaan, Liechtenstein) block. For proper shade mapping polarized picture with grey card for digital calibration is necessary as well as one standard picture for mapping the color effects. For tooth color mapping we used the eLAB software (eLAB Prime, Freiburg im Breisgau, Germany). Highest ?Eab value for all three cases was 2.7 or less, which indicates that the color is clinically acceptable, considering acceptability threshold value of less than 2.7 (three-year follow-up confirmed acceptable color appearance. Conclusion Following recommended protocol based on the eLAB software software (eLAB Prime), clinically acceptable color of the all-ceramic crown were obtained.

Colorimetric and fluorescent dual-identification of Methicillin-Resistant Staphylococcus aureus (MRSA) using programmable CRISPR/Cas12a system

Accurate identification of Staphylococcus aureus (S. aureus) is crucial for food safety and clinical diagnosis. Particularly, identifying drug-resistant strains guides effective treatment and prevention of infectious diseases. Herein, we presented a highly efficient programmable CRISPR/Cas12a system for identification of methicillin-resistant S. aureus (MRSA). Genus-specific gene (spec gene) and methicillin-resistant gene (mecA gene) of S. aureus were amplified by PCR. According to the sequences of spec gene and mecA gene, corresponding programmable crRNAs were designed and prepared to construct CRISPR/Cas12a system, which recognized and bound to the PCR products through complementary interactions to activate the trans-cleavage activity of Cas12a. This led to the indiscriminate digestion of surrounding FQ-reporter, releasing a distinct fluorescence signal for MRSA identification. In addition, we devised a pair of AuNPs-DNA probes for colorimetric recognition of S. aureus. Target-triggered trans-cleavage for linker probe led to an non-aggregation of AuNPs-DNA probes pair. This led to a noticeable appearance of red color, enabling naked-eye detection. Thus, MRSA identification was realized with remarkable sensitivity and exceptional selectivity. Successful identification of MRSA in clinical samples validated the practical applications of the proposed CRISPR/Cas12a assay, establishing it as a universal platform for diagnosing pathogenic microorganisms with versatility and compatibility.

Translocation and survival of trunk injected Beauveria bassiana (Hypocreales: Cordycipitaceae) in healthy date palm trees

ObjectiveThe date palm, Phoenix dactylifera L., is an ancient and valuable tree that provides food and other products. The date palm trees are attacked by several pests, including the red palm weevil, which is devastating to date palm plantations. Knowledge of the functionality of entomopathogenic fungi, including Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) species, in the tree trunk is critical for controlling date palm weevil and other pests. The goal of this study was to assess the movement of the entomopathogenic fungus, B. bassiana, within the date palm tree. MethodsBeauveria bassiana (BbSA-4) mixed with food colors was trunk-injected using a balloon injector into healthy date palm plants. Trunks were cut into one-meter logs 2, 20, and 86 days post-injection. Each log was further dissected into four quarters to examine the present of the fungus. The appearance of food colors and the detection of fungal spores at different heights from the point of injection revealed the translocation of B. bassiana within the trunk. The samples were taken from several locations where food color could be visible, and the distance traveled by the fungal spores was measured. ResultsThe injected palm tissue samples were cultured on PDA media in the laboratory, and the present of fungal spores was confirmed. B. bassiana (BbSA-4) was found to be surviving in all treated date palm trees. The survival rate of isolate BbSA-4 averaged 70.5%, 34.9%, and 13.9% from dissected trunks examined at 2, 20, and 86 days, respectively, after injection. Isolate BbSA-4 was more apparent in the trunk after spiral injected than bottom injection. The findings revealed that using an entomopathogenic fungus as an endophytic to supplement IPM programs could be beneficial.

Colour prediction as a weaving design selection help tool in Jacquard CAD

The final colour prediction of a weave design made of dyed yarns is a difficult problem. This study shows how a geometric model can be developed to obtain the final colour prediction objectively. For this purpose, a woven material was divided into weft, warp and pores. Then, all parameters needed for the calculation of each colour contribution were identified. A geometrical model based on construction parameters was developed to predict the surface colour contribution of each coloured yarn in a weave surface. To validate the predicted colorimetric data, a visual assessment experiment was conducted. Then, the difference between the predicted and actual colour appearance of the weave pattern was evaluated and analysed in function of weaving structures, and weft yarns colours. For this purpose, simple woven structures (plain, twill 1/3, basket 2/2 and satin Turc) with four coloured weft yarns were used. Results show that the proposed model could correctly predict the final colour of weave designs. Therefore, the model has the potential to eliminate subjective evaluations and reduce prototype sample production by automating the process of weave/colour simulation, thereby reducing the cost and time for product development. The methods of utilization of colour in woven textiles depend upon the composition of the weave design to be woven and the structure parameters of the cloth.

A Method of Enhancing Silk Digital Printing Color Prediction through Pix2Pix GAN-Based Approaches

Color prediction and color management for digital printed fabrics remain a challenging task. Accurate prediction of color appearances of digital printed fabrics would enable designers and manufacturers to better fulfill their design requirements and creative visions. We propose a color prediction method for silk digital printing utilizing a Pix2Pix Generative Adversarial Network (GAN) framework. This method aims to generate predicted images that possess the same stylistic and color characteristics as their actual fabrics after production. To develop and validate the method, color data and images are collected and processed from 5252 sets of paired original Pantone TPX color card and actual print sample fabrics. The results of this study demonstrate that the method can predict the colors of silk digital print samples while effectively reproducing the effects of inkjet printing in silk fabrics including silk crepe satin and silk twill. The method exhibits high prediction accuracy to an average CIEDE2000 value of 2.372 for silk crepe satin and 1.846 for silk twill. The findings of this research not only enhance the efficiency and accuracy of color management in fabric digital printing technology but also contribute to the exploration and development of high-fidelity color prediction techniques within the textile industry.

Valorization of Potato Peel Waste as Natural Additive for Use in Meat Products

Potato peel is a waste generated in large amounts in the food industry; however, it has been shown that these residues are an important source of antioxidant compounds. The effect of potato peel powder addition (2, 5, and 10%) on the physicochemical, sensory, and antioxidant status of pork patties during refrigerated storage (2 °C/9 days/under dark) was evaluated. Polyphenol content and antioxidant activity of potato peel powder ethanol extract were determined. Pork patties were subjected to proximate chemical composition, physicochemical, and sensory evaluations. Results showed that potato peel ethanol extract at the highest used concentration (500 µg/mL) is an important source of total phenolic (>50 mg gallic acid equivalents/g) and chlorogenic acid compounds (ca. 40 mg chlorogenic acid equivalents/g) and exerts free radical scavenging (>50% of DPPH inhibition) and reducing power activity (<0.5 abs) (p < 0.05). Additionally, potato peel powder incorporation in raw pork patties reduces changes in pH, lipid oxidation, water-holding capacity, cooking loss weight, and color values during storage. Although an effect was observed on texture and sensory values (color and appearance) of raw patties, depending on addition level (p < 0.05), no differences were found in color appearance, odor, flavor, juiciness, fat sensation, texture, and overall acceptability of cooked patties between treatments (p > 0.05). The use of potato peel powder as a natural antioxidant for meat products is recommended.

Sensory quality of beef with different ultimate pH values – A Brazilian perspective

This study evaluated Brazilian consumer perceptions of beef with different pH values at 48 h post-mortem (pHu) through sensory analysis. A total of 138 consumers evaluated raw and grilled steaks. The steaks were divided according to their pHu (normal pH < 5.8, atypical darkness, firmness, and dryness [DFD] > 5.8 pH < 6, and typical DFD pH ≥ 6). There was no difference in the visual evaluation of raw steaks or purchase intention. Evaluation of the grilled steaks showed that consumers preferred typical DFD steaks in terms of tenderness, and there was a tendency for the same behavior in terms of juiciness. No differences were observed in other evaluated parameters. Cluster analysis identified three consumer segments for visual evaluation, indicating a preference for steaks with higher pHu in terms of freshness. Additionally, four segments were identified for evaluation, with some groups expressing a preference for higher pHu meat in terms of freshness appearance, tenderness liking, and overall liking. The developed regression models for overall liking and purchase intention exhibited favorable adjustment indices, with r2 values of 0.86 and 0.57, respectively, for raw steaks and 0.90 for grilled steaks for in overall liking. Regression models indicated a strong influence of color and freshness appearance, in addition to tenderness liking and juiciness liking, on the overall liking scores of consumers. These results indicate that Brazilian consumers do not dislike dark cutting and, despite differentiating their greater tenderness, do not show a preference between the different pHu values.

Contributed Session II: Computational modeling of shift in unique yellow for small stimuli.

Unique yellow (UY) is largely invariant to L:M cone proportion for spatially-extended stimuli in healthy trichromats. However, a recent adaptive-optics-based study by Boehm et al. reveals that when stimulus size is reduced to a few arcmin, color appearance depends on the local L:M proportion in the patch of the retina on which the stimulus was imaged. We aimed to determine if such findings are consistent with a normative account of visual processing. A series of 3.5 and 10 arcmin stimuli were simulated as isoluminant mixtures of 540 and 680 nm primaries. We modeled sensory encoding under adaptive-optics conditions using the open-source software ISETBio, for simulated retinal cone mosaics with varying local L:M proportions. The resultant cone excitations were decoded using a Bayesian image reconstruction algorithm (Zhang et al., 2022). For the 3.5 arcmin stimuli, as local L:M proportion decreased, the 540 nm component of the reconstructions increased relative to the 680 nm component. This is qualitatively consistent with the experimental observations of Boehm et al. For 10 arcmin stimuli, in contrast, reconstructions were stable across variation in local L:M cone proportion. Notably, reconstructions depend not only on the local L:M cone proportion, but also on the proportion in the immediately surrounding retina, leading to a testable prediction. The computational observations frame the experimental results as a normative consequence of visual processing.

Poster Session II: Spillover effects of color discrimination training on color category boundaries and color appearance.

Perceptual learning refers to the increase in perceptual sensitivity that results from several days of training on a perceptual task. Although perceptual learning has been shown to be effective in a variety of perceptual tasks, few studies have examined perceptual learning in color perception. In this study, we investigated how color discrimination training at a base color affected various aspects of color perception for entire hues. The training consisted of five days of S color discrimination (300 trials/day) at either the negative or positive L-M base color, depending on the observer groups. Before and after the training, three types of color perception tests (color difference, unique hue, and color category boundary) were conducted for colors with various hues to examine the changes in color perception due to the training. The results showed that the color discrimination thresholds in the training decreased as expected with repeated trials. Interestingly, the training also affected the performance of the three types of tests; the perceived color difference around the training color tended to increase, and some of the unique hues and the color category boundaries shifted significantly toward the training color. These results suggest that only a few days of color discrimination training can spill over to the entire color space and induce distortion of the perceptual color space.

Improving the Physicochemical and Sensory Properties of Supported Ice Cream Fortified with Dates by Adding Kappa-Carrageenan

The aim of the research is to introduce kappa-carrageenan in the manufacture of ice cream instead of carboxymethyl cellulose and to know its effect on the chemical and biological properties. three replicates of the control treatment T1, two replicates of the treatment in which 50% of the CMC was replaced with kappa-carrageenan T2, and three replicates of the treatment in which all of the CMC was replaced with kappa-carrageenan T3. The chemical composition of Zuhdi dates were (70.69, 59.20, 2.68, 0.47, 2.57, and 18.28 %) for each of the total sugars, reducing sugars, protein, fats, total ash and moisture (respectively). The physical examinations of Al-Zuhdi dates showed that the solids value was 75.00, the refractive index was 1.3560, the color intensity was 1.50, and pH was 6.60. pH was in between T1 and T3 amounted to 6.6 and 6.8, respectively, and the total acidity between T1 and T3 were 0.23 and 0.20, respectively, and the results showed no significant differences for the total solids, they were (28, 29, and 30%) and the refractive index was 1.3810, 1.3811, and 1.3837. Sensory tests showed that there were significant (P≤0.05) differences for the ice cream in texture, composition, resistance to melting, and no significant differences appeared in taste, color, and external appearance, and there was no growth of microorganisms and total number of bacteria, Psychrophile bacteria, coliform bacteria, yeasts, and molds.

The colourful optical appearance of centric diatom fossil frustules with diagenetic nanocrystalline calcite fill

The colourful optical appearance of centric diatom fossil frustules with diagenetic nanocrystalline calcite fill

Evaluation of shade correspondence between high-translucency pre-colored zirconia and shade tab by considering the influence of cement shade and substrate materials

Statement of problemThe relation between cement color and abutment substrate material and the corresponding effect on the color accuracy of high-transparency pre-colored zirconia (HT-Zr) remains unclear. PurposeThis in-vitro study aimed to investigate the difference in color accuracy when the HT-Zr is bonded to different materials-based substrates with differently colored resin cement. Materials and methodsVita A1 shade HT-Zr with 1 mm thickness was used as the testing sample. The samples were first placed on zirconia (ZR), tooth color resin (CR), and metallic (MT) abutment substrates. Subsequently, four differently colored cements (translucent (TR), bleach, opaque, and A2 shade (A2)) were used for bonding HT-Zr onto the substrate, and the non-bonded group was used as the control group (CG). There were 15 groups in total (n = 10 per group). A digital colorimeter was used to obtain Commission Internationale de l’Eclairage (CIELab) color parameters. The translucency parameter (TP00) of the substrate and sample, as well as color difference (ΔE00) and chroma (C) between the different groups were calculated. Additionally, the ΔE00 and TP00 were compared with the moderately unacceptable match of ΔE00 = 3.6. The statistical analysis was conducted using ANOVA and Tukey HSD post-hoc test (α = 0.05). ResultsHT-Zr exhibited high translucency (TP00 = 11.02 ± 0.18), and the mean ΔE00 of the testing samples ranged between 2.18 ± 0.20 and 13.14 ± 0.31. The ZR-CG and MT-A2 groups showed the highest and lowest lightness separately. The CR-CG group exhibited the highest C, and the ΔE00 was lower than that of 3.6. The MT-TR group showed the lowest C and the highest ΔE00. The inter-group comparison revealed that the ΔE00 for different cement is mostly lower than the acceptable color match of 1.0; moreover, the ΔE00 for all the substrates, excluding the CG group, is higher than 3.6. ConclusionsThe abutment substrate materials and the cement color should be considered with caution when using HT-Zr, with the effect of abutment substrate materials being more apparent in color accuracy. HT-Zr restorations are not recommended for discolored or bleached abutments but only for natural-colored abutments to achieve the optimal color appearance.

Effects of gamal leaf extract (Gliricidia sepium Jacq.) on body weight and internal organ performance of mice (Mus musculus L.)

Gamal leaves (Gliricidia sepium Jacq.) contain toxic compounds that have the potential to act as vegetable rodenticides, such as alkaloids, tannins, dicumerol, and hydrogen cyanic acid (HCN). Coumarin compounds can bind vitamin K, disrupt the circulatory system, and damage liver cells. The compounds dicumerol and warfarin are used as rat poison because of their anticoagulant properties, which prevent blood clots in the heart. Alkaloid and tannin compounds can damage liver cells through liver necrosis and bleeding in the digestive system. This study aims to determine the effect of Gamal leaf extract on body weight and internal organ performance in mice (Mus musculus L.). The experimental research method is based on a Completely Randomized Design (CRD) pattern. A total of 16 mice weighing 20-22 grams consisting of 4 treatments, namely A0 (water control), A1 (extract 10 mg/g BW), A2 (extract 15 mg/g BW) and A3 (extract 20 mg/g BW). The extract was given orally for 14 days, and body weight was measured every 3 days to obtain data on changes in body weight (PBB). At the end of the treatment, the mice were sacrificed using chloroform and then dissected to observe the performance of the liver, kidney and heart organs in color appearance and organ weight. Quantitative data were analyzed using one-way ANOVA and further DMRT test α=0.05. The research showed that mice's mean change in body weight (PBB) decreased significantly as the dose of Gamal leaf extract increased. The percentage of liver organ weight (BOH), kidney organ weight (BOG), and heart organ weight (BOJ) decreased as the dose of Gamal leaf extract increased. The research results concluded that Gamal leaf extract caused color changes in the internal organs of mice and significantly affected the percentage change in body weight (PBB) and internal organ weight (BOD) of mice along with increasing doses of Gamal leaf extract.

Organoleptic Analysis, Quality Requirement, And Color Determination of Tilapia Nuggets with Eleutherine palmifolia Extract Coating

Nuggets are a product that should be stored frozen, but nuggets are often sold at inappropriate temperatures, causing physical changes such as pale color. Dayak onion extract can improve color appearance and provide added antioxidant value to tilapia nuggets. Quality testing of tilapia nuggets with Eleutherine palmifolia (Dayak onion) extract coatings has been investigated. The objectives of this research are to apply Dayak onion extract to improve the appearance of tilapia nuggets and to obtain tilapia nuggets with Dayak onion extract that meets SNI requirements. The results of organoleptic tests showed that panelists preferred tilapia nuggets with Dayak onion extract over commercial controls and nuggets. The panelists preferred tilapia nuggets with 100 ppm ethanol extract and 10000 ppm aqueous extract, both continued to the SNI conformity test. The sensory evaluation results based on SNI showed that the appearance of the tilapia nuggets with 100 ppm ethanol extract was significantly different. Adding Dayak onion extract to tilapia nuggets affected the lightness and yellowness of tilapia nuggets and gave a 'moderate orange' color to the tilapia nuggets with ethanol and aqueous extract. The selected tilapia nugget with 100 ppm aqueous Dayak onion extract meets all chemical, microbiological, and sensory requirements as SNI 7758:2013 concerning fish nuggets. Tilapia nuggets with Dayak onion extract had a brighter Moderate orange color and were preferred by panelists compared to controls and commercial fish nuggets that were moderate dark orange. Applying Dayak onion extract on tilapia nuggets can improve the appearance and it potentially contains natural antioxidants.

The apple transcription factor MdbHLH4 regulates plant morphology and fruit development by promoting cell enlargement

The bHLH family, the second largest transcription factor (TF) family in plants, plays a crucial role in regulating plant growth and development processes. However, the biological functions and mechanisms of most bHLH proteins remain unknown, particularly in apples. In this study, we found that MdbHLH4 positively modulates plant growth and development by enhancing cell expansion. Overexpression (OE) of MdbHLH4 resulted in increased biomass, stem and root length, leaf area, and larger areas of pith, xylem, and cortex with greater cell size compared with wild-type apple plants. Conversely, RNA interference (RNAi)-mediated silencing of MdbHLH4 led to reduced xylem and phloem as well as smaller cell size compared to wild-type apple plants. Ectopic expression of MdbHLH4 in tomatoes resulted in enlarged fruits with impaired color appearance, decreased accumulation of soluble solids, and decreased flesh firmness along with larger seeds. Subsequent investigations have shown that MdbHLH4 directly binds to the promoters of MdARF6b and MdPIF4b, enhancing their expression levels. These findings suggest that MdbHLH4 potentially regulates plant cell expansion through auxin and light signaling pathways. These study results not only provide new insights into the roles of bHLH transcription factors in regulating plant growth and development but also contribute to a deeper understanding of their underlying mechanisms.

High-dynamic-range Colour Appearance Data to Verify CAM16-UCS

High-dynamic-range Colour Appearance Data to Verify CAM16-UCS

Effect of primary peak wavelength and stimulus size on metameric failure through color difference evaluations

AbstractTristimulus values and chromaticities, which are derived using the color matching functions (CMFs), are commonly used for color characterization, calibration, and specifications, with the stimuli having the same values believed to have the same color appearance (i.e., metameric match). Many studies, however, found that the stimuli having the same tristimulus values do not appear the same (known as metameric failure) due to the failure of CMFs in accurately characterizing the color matching mechanisms. Most past work investigated the performance of different CMFs through color matching experiments, with a smaller chromaticity or calculated color difference between the reference and test stimuli suggesting a better performance. Such differences, however, may not accurately characterize the performance of the CMFs, since the color spaces or chromaticity diagrams may not be uniform, in terms of the threshold of noticeable color difference. In this study, the human observers evaluated the perceived color difference between pairs of stimuli, which were calibrated to have the same tristimulus values calculated using the CIE 1931 2° CMFs, with two sizes of field of view (FOV). The results clearly suggested that the stimuli having the same tristimulus values may not always appear the same, which depended on the primaries and the FOV. More importantly, the results clearly suggested that the chromaticity differences from color matching experiments may overestimate the metameric failure, especially when the color differences were small.

The brightness of chromatic stimuli

AbstractWe present the results of a series of three brightness‐matching experiments which include a key refinement in this established psychophysical method. Constraining the chroma difference between pairs of stimuli matched in brightness allows us to use a novel calculus‐based approach to building a model of the brightness of chromatic visual stimuli and the Helmholtz‐Kohlrausch effect. The proposed models, compatible with the CIECAM16 color appearance model and CIELAB color space, are compared to other models of the Helmholtz‐Kohlrausch effect on these data and other past brightness‐matching studies. The proposed CIECAM16‐based model demonstrates the overall best performance on predicting the Helmholtz‐Kohlrausch effect in four newer data sets but overpredicts the weaker effect reported in three older data sets. The potential experimental factors in this divide between new and old studies are also discussed.