ABSTRACTThe present study investigated the effects of dietary supplementation of multienzyme preparation on growth performance, apparent total tract digestibility (ATTD) of nutrients, and gut health of weaned pigs. A total of 240 weaned pigs (initial body weight: 7.3 ± 0.7 kg) were randomly allotted to five treatments on the basis of body weight. The dietary treatments included a corn–soybean meal (SBM)-based diet supplemented with 0 (control), 0.025, 0.050%, 0.075%, or 0.10% multienzyme preparation. The experimental diets were fed in a meal form for two phases (d 0–14, phase I and d 15–28, phase II). Increasing multienzyme supplementation linearly improved overall (d 0–28) average daily gain, gain:feed, and ATTD of dry matter, gross energy, and crude protein. At d 21, pigs fed dietary increasing levels of multienzyme preparation had linearly greater Lactobacillus spp. (ileum and caecum and faeces) and fewer Clostridium spp. (ileum and caecum) and Escherichia coli (ileum and faeces) populations. On d 28, villus height of the jejunum and ileum were linearly increased with dietary increase in multienzyme preparation. In conclusion, this multienzyme has potential to improve the growth performance, ATTD of nutrients, and gut health of weaned pigs fed a corn–SBM-based diet in the absence of antibiotics growth promoters.