Apparent Metabolizable Energy Research Articles

Efeito do tamanho de partícula da farinha de ostras e relações cálcio: fósforo em dietas para frangos de corte

ABSTRACT: The effects of Ca:P total ratio and particle size of oyster shell meal (OSM) were evaluated in broiler diets. In Experiment 1, 800 broilers (22-42 days old) were distributed in a 2×2 factorial design, with two Ca:P ratios (1.7 and 2.0:1) and two OSM particle sizes (coarse = 1,354 µm and fine = 428 µm), totaling four treatments with 10 repetitions with 20 broilers. Feed intake, weight gain, and feed conversion ratio were calculated. In Experiment 2, 1,280 broilers were distributed in a 2×2×2 factorial design (1.7 and 2.0:1 Ca:P ratios; coarse and fine OSM; male and female broilers), with eight treatments and 16 repetitions with 10 broilers. Apparent metabolizability of dry matter, Ca, P, and apparent metabolizable energy (AME), as well as bone resistance, bone weight, ash, Ca, and P content in the tibia were assessed. Growth performance was not affected (P > 0.05). Coarse OSM increased tibia Ca content in male broilers (P < 0.001), and higher Ca:P ratio improved bone ash and bone resistance in both sexes (P < 0.001), but reduced P content in male broilers (P < 0.05); male broilers displayed heavier bones with higher ash content than females (P < 0.05). Metabolizability of Ca was improved with coarse OSM (P < 0.05); whereas metabolizability of DM, P, and AME was not affected (P > 0.05). In conclusion, diets with a Ca:P total ratio of 2.0:1 containing coarser OSM improved bone mineral composition, particularly in male broilers, and coarse OSM improved the metabolizability of Ca in broilers regardless of the Ca:P total ratio or broiler sex.

Near-infrared spectroscopy to assess mycotoxins contamination and nutritional composition of maize marketed in South America, years 2020-2021

Mycotoxicological and nutritional analyses were conducted in maize from Argentina, Bolivia, Brazil (stratified by region), Colombia and Peru through near-infrared spectroscopy (NIRS) in 2020 and 2021. Concentrations of aflatoxin B1 (AFB1), fumonisins B1 + B2 (FBs), zearalenone (ZEN) and deoxynivalenol, in addition to water activity, crude protein (CP), ether extract (EE), starch and apparent metabolizable energy (AME) in poultry, were determined in 18,363 spectra (totalling 195,486 analyses). FBs were the most prevalent metabolites in South American maize, being detected in 91.6 and 91.9% of the samples; ZEN had the second highest positivity, 15.4 and 26.8%, followed by AFB1, 15.3 and 14.6% (2020 and 2021, respectively). FBs also had the highest incidence in Brazilian maize, 92.6 and 92.1%, followed by AFB1, 15.2 and 13.5%, and ZEN, 14.7 and 27.7% (2020 and 2021, respectively). Contamination with at least one mycotoxin was detected in 93.5% of the samples. The mycotoxins found co-contaminating the maize belong to the generaFusarium andAspergillus; FBs, followed by ZEN and AFB1, were the most prevalent toxins in the associations. Levels of water activity were below 0.70. Regarding chemical composition, CP presented the largest oscillation in relation to the mean in South American maize (both years). As for the Brazilian samples, the Southeast region showed the greatest positive variability in relation to the means for all evaluated parameters, followed by the Northeast region (excepting CP) in 2020; in 2021, the Central-West and Northeast regions had the highest levels of EE and AME. In both years, the South region presented lower levels in relation to the mean for most parameters. Data on prevalence, mycotoxicological contamination, storage conditions and nutritional quality of maize assist the decision-making process of raw material use. In this setting, NIRS suits the needs of the industry for providing information on multiple parameters in real time.

Effects of a Novel Protease from Bacillus Subtilis K-5 in Low Protein Corn Distiller Dried Grains with Solubles (cDDGS) Based Diets on Performance and Nutrients Digestibility in Broiler Chickens

This experiment was conducted to evaluate the supplemental effects of a novel protease produced from Bacillus subtilis in low crude protein (CP) corn distiller dried grain with solubles (cDDGS) based diets on growth performance, carcass attributes, nutrients digestibility, blood chemistry, and intestinal histomorphometry of broiler chickens. One hundred and sixty, one-day-old chicks were randomly allotted to one of 4 dietary treatments. Each dietary treatment had four replicates, with 10 birds in each replicate. Two basal diets were formulated for both starter (1-21d) and finisher (22-35d) phase; (PC) a corn soybean meal based diet as per standard recommendations of Ross 308; (NC) 5% cDGGS with 5% reduction in CP with concomitant reduction in essential amino acids (EAAs) compared with PC. The negative control diet was further subdivided into 3 parts. One part was without enzyme supplementation, while the other two parts were supplemented with a novel protease (PROT1) and a commercial protease (PROT2), respectively. The same procedure was adopted for finisher diets. A digestibility assay (32-35d) was carried out using acid insoluble ash (AIA), an external digestibility marker. At the end of 35d, ileal digesta were collected from four birds per experimental unit for nutrient digestibility measurement. Tissue samples of duodenum, jejunum, and ileum were collected for villus height, villus width, crypt depth, and crypt width. Body weight gain (BWG) and feed:gain were improved (p<0.05) with protease supplementation. No effect was observed on carcass parameters. However, CP digestibility, apparent digestibility coefficient for nitrogen (ADCn), nitrogen retention (Nret ), and apparent ileal amino acid digestibility (AIAAD) were improved (p<0.05). However, there was no effect on apparent metabolizable energy (AME) and apparent metabolizable energy corrected for nitrogen (AMEn), blood glucose, total protein and cholesterol (p>0.05) and intestinal integrity (p>0.05). It was concluded that protease enzyme can improve nitrogen and CP digestibility, resulting in improved amino acids availability in low protein diets.

Metabolizable and Net Energy Values of Expanded Cottonseed Meal for Laying Hens and Broiler Chickens.

Three experiments were conducted to determine the metabolizable energy (ME) and net energy (NE) values of expanded cottonseed meal (ECSM) for broilers aged 14–16 days (Experiment 1), broilers aged 28–30 days (Experiment 2), and 45-week-old Hy-Line Brown hens (Experiment 3). Reference diets based on corn-soybean meal were used to meet the nutritional needs of the birds. The test diets contained ECSM as basis, which was used to replace 18.5% of the gross energy-yielding ingredients from the reference diet. The birds were fed a commercial feed before the experimental period. After the dietary adaptation period, six birds per replicate (Experiment 1) and two birds per replicate (Experiments 2 and 3) for each treatment group were placed in an individual open-circuit respiratory calorimetry chamber for 3 days. Daily O2 consumption and CO2 production were recorded, and excreta samples were collected. The ME and NE values of ECSM were determined using the substitution method. The apparent metabolizable energy (AME) values of ECSM for experiments 1, 2, and 3 were 2605.85, 2178.31, and 2782.60 kcal/kg of dry matter (DM), respectively. The NE values were 1655.23, 1196.64, and 1538.19 kcal/kg of DM, respectively. The NE:AME ratios of ECSM were 63.52%, 54.93%, and 55.29%, respectively. Our data showed that the ME and NE values of ECSM differed across various growth stages and types of chickens. These results demonstrate that the appropriate ME and NE should be used in the design of different feed formulas for specific growth stages and types of chickens.

Enzyme complex addition in barley or rye broiler diets with two energy levels fed from 1 to 21 days

Two experiments were conducted to evaluate diet digestibility, performance, digestive parameters, and blood parameters when an enzyme complex (EC) was used in barley- and rye-based diets with different energy levels. In the digestibility assay (exp. I), 108 seventeen-day-old Cobb male broilers were distributed in a completely randomized design in 2 × 2 × 2 + 1 factorial arrangement with two feeds (barley or rye), two EC levels (0% and 0.02%), and two energy levels [3025 and 3125 kcal apparent metabolizable energy (AME)·kg−1], plus a control treatment. In exp. II, 1080 one-day-old Cobb male broilers were distributed in a completely randomized design in 2 × 2 × 2 + 1 factorial arrangement with two feeds (barley or rye), two EC levels (0% and 0.02%), and two energy levels (2875 and 2975 kcal AME·kg−1). No interactions were observed for any variables (exp. I and II). Enzyme complex improved the apparent metabolizable coefficient of gross energy (P = 0.0432) of diets. The EC provided greater weight gain (P = 0.0003) and better feed conversion (P = 0.0025). Intestinal viscosity at 21 d was reduced (P &lt; 0.0001) with the addition of the EC. The EC improved nutrient digestibility and performance, but the effects of energy reduction on performance could not be overcome.

Colombian vs. American corn: Quality, chemical composition, mycotoxin content and metabolizable energy

Background: Corn is, quantitatively, one of the most important world crops (ranking second only after wheat) and a key ingredient in animal feeds. Objective: to assess and compare corn quality, mycotoxin content, chemical composition and apparent metabolizable energy (AME) of domestic and imported corn. Methods: Grain quality (USDA grading system) was determined in 30 samples of domestic and 21 samples of imported corn. From each origin, 15 samples were subjected to proximal analysis and 10 were used to determine fatty acid composition. Mycotoxin analysis was conducted on 30 samples of domestic and 23 of imported corn. Results: six of the 30 domestic samples corresponded to US1 grade (highest quality) vs. none of the imported. In the “sample grade” category (lowest quality), 10 and 6 samples corresponded to imported and domestic corn, respectively. Soybeans were found as contaminant in 15 of the 21 imported corn samples. Aspergillus spp. mycotoxins such as ochratoxin A were not detected, and aflatoxins were found in only a few samples at very low levels. Fusariotoxins such as deoxynivalenol and zearalenone were found in 61 and 43% of imported samples, respectively, but in none of the domestic samples. Domestic corn had lower carbohydrate content compared with imported corn (85.4 vs. 86.7%), but higher crude fat (3.8 vs. 3.1%). The AME values for domestic and imported corn were 3,697 and 3,378 kcal/kg, respectively. The fatty acid profiles from both corn types were similar. Conclusion: This study found significant differences between locally-grown and imported corn, particularly in terms of crude fat, AME content, fusariotoxins, and contaminant seeds (soybeans). These findings suggest that locally-grown corn might have nutritional and toxicological advantages over corn imported from the United States.

Passion fruit seed cake in the feeding of meat quails: effects on performance, carcass characteristics, lipid stability of the meat, litter quality, and economic viability.

The objective of this study was to determine the chemical composition and energy value of passion fruit seed cake (PFSC) and to evaluate the inclusion of different levels of PFSC in the diets for quails on growth performance, carcass quality, litter quality, lipid stability of the meat, and economic viability. In the metabolism assay, 108 European quails of 21days of age were used, being distributed in a completely randomized design with 3 treatments (one reference ration and two test rations with substitution of 20% and 40% of the reference ration by PFSC) and 6 replications of 6 birds each. It was observed that the apparent metabolizable energy corrected by the nitrogen balance (AMEn) determined in the ration with 20% PFSC (3876.17kcal/kg DM) was higher than in the 40% (3469.00kcal/kg DM). In the performance assay, 432 European quails from 7 to 42days of age were used, being distributed in a completely randomized design with 6 treatments, consisting of a control diet and the others with increasing levels of inclusion of passion fruit seed (5, 10, 15, 20, and 25%), and 6 replications of 12 birds. It was observed that the inclusion of PFSC starting from 10% promoted increase FI and FC values, and increasing linear effect of these variables starting from 5% of inclusion. The PFSC did not affect the carcass characteristics and lipid oxidation of the meat. Regarding the litter quality, there was a linear increase in the pH values and worse economic indexes starting from the inclusion of 10% of PFSC. In conclusion, PFSC can be used up to the level of 5% in diets for meat quails from 7 to 42days of age.

The Impact of Genotype and Age on Energy and Protein Utilization in Individually Housed Brown Laying Hens.

Simple SummaryAn investigation into whether the nutrient requirement of egg-laying hens has changed with genotype improvements was conducted. This study compared the response of individually housed laying hens of two different genotypes and ages. The strains used were a heritage breed in mid-lay and a modern breed at peak production. Energy was utilized with the same efficiency by both strains. All hens were able to adjust their feed intake to ensure that their energy requirements were met. The modern strain utilized protein slightly more efficiently than the heritage strain. It is unlikely that the nutrient requirements of modern layer strains have increased. More likely, requirements have decreased because modern hens are lighter and have a lower daily egg output (lay smaller eggs) despite their higher lifetime egg output. Regardless, feeding programs need to be adjusted for economic reasons and depend on achievable feed intakes under particular circumstances.Responses to dietary energy and protein levels were compared between two egg-laying genotypes. Individually housed hens of a historic strain (HS) and a modern strain (MS) were compared. In Experiment 1 (Exp.1), four levels of true metabolizable energy, corrected for zero nitrogen retention (TMEn) and four levels of total lysine, were offered from 30 to 40 weeks of age. In Experiment 2 (Exp.2), three levels of apparent metabolizable energy, corrected for zero nitrogen retention (AMEn) and four levels of standardized ileal digestible lysine (SID Lys), were fed from 20 to 30 weeks of age. A randomized factorial block design (4 × 4 Exp.1 and 3 × 4 Exp.2) was applied. Energy utilization for egg output (EO) did not differ (NS), and both strains maintained a constant kJ intake (NS). The efficiency of SID Lys utilization for EO differed, with the MS being the more efficient (p < 0.034). A single model could be used to predict feed intake, using BW, EO, AMEn and SID Lys (r = 0.716). In conclusion, it is unlikely that the requirements of modern layer strains have increased. However, feeding programs should be adjusted for economic reasons and are dependent upon achievable feed intake under particular circumstances.

Composition and availability of nutrients of three corn hybrids dried at different temperatures in the diets of broiler chickens

The objective of this study was to determine the chemical composition, non-starch polysaccharides (NSP) content, in vitro digestibility of starch, apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and apparent and apparent nitrogen-corrected coefficient of metabolizable energy (ACME and ACMEn) of three corn hybrids dried at different temperatures in the diets of broiler chickens. The energetic values were determined by the total collection of excreta method, using broilers from 11 to 21 d, placed in a completely randomized design in [...]

Propolis Supplementation on Broiler Chicken Performances, Nutrient Digestibility, and Carcass Characteristics: A Meta-Analysis

This meta-analysis was conducted to investigate the effect of dietary propolis supplementation on broiler performance, carcass characteristics, and nutrient digestibility evaluated at the starter and finisher phases. An online literature search was conducted using scientific platforms of Science Direct, PubMed, and Google Scholar to identify the studies utilizing propolis as dietary supplementation in broiler chickens. A total of 39 publications comprising of 58 experiments met the inclusion criteria. A mixed model procedure for meta-analysis was performed, considering the studies as a random effect and levels of propolis as a fixed effect. Regression analysis suggested that average daily gain (ADG), body weight, and average daily feed intake (ADFI) at starter and finisher phases were quadratically affected by supplementing propolis in the diet (p<0.01). Propolis also lowered the feed conversion ratio (FCR) throughout the periods (p<0.05). In addition, a positive effect of propolis inclusion was also observed on breast meat percentage (p<0.05). However, there was a negative linear relationship between propolis and the digestibility of dry matter (DMD) and organic matter (OMD) (p<0.01) at the starter period. Conversely, there was a linear increase in DMD (p<0.05) and a tendency to improve apparent metabolizable energy (AME) and phosphorus digestibility at the finisher period (p<0.10). Overall, it can be concluded that dietary propolis supplementation promoted better growth performance and feed efficiency when given at ~1.66-2.13 g/kg diets for starter and finisher phases in the broiler chickens. The effect of propolis on nutrient digestibility was positive at the finisher phase while it was detrimental at the starter phase.

The Apparent Metabolisable Energy Values of Palm Kernel Cake as Influenced by Enzymes and Cocktails

This study was conducted to quantify the effects of enzymes and their cocktails on the apparent metabolisable energy (AME) value of palm kernel cake to maximise enzymes’ advantages. There were eight treatments, each with three replicates in a completely randomised design with T1 as control. The experimental treatments had Xylanase, Multipurpose and phytase enzymes respectively for T2, T3 and T4, and a cocktail of xylanase and multipurpose, a cocktail of xylanase and phytase, cocktail of multipurpose and phytase, and cocktail of xylanase, multipurpose and phytase for T5, T6, T7 and T8. The feeding trial was done using the intubation method. Gross energy values were determined with calorimeter while calculated AME values were subjected to analysis of variance using Statistical Analysis System and treatment means separated by Duncan Multiple Range Test. Results show that individual enzymes, apart from phytase, significantly (p&gt;0.05) improved the AME value of palm kernel cake. Treatment T8 was significantly (p&gt;0.05) better than the other treatments. Each cocktail was significantly better than its respective individual enzymes except cocktail of multipurpose enzyme and phytase that was not significantly different from multipurpose enzyme but significantly (p&gt;0.05) better than phytase. It was concluded that enzymes and cocktail of enzymes could be used to enhance the energy content of nonconventional feedstuffs thereby serving as a replacement to conventional energy feedstuffs.

Nutrient Metabolisability of Treated False Yam (Icacina Oliviformis) Seed meal for Broiler Chickens

A study was conducted to evaluate apparent metabolizable energy (AME) and nutrient metabolisability (NM) of maize-based diets containing treated false yam seed meal (TFYSM) using a 5x6 factorial [5 treatment samples (Un_T, Urea_T, NaCl_T, NaOH_T and KOH_T) at 6 levels (0, 10, 20, 30, 40 and 50) each] design for 15 days. Matured false yam (Icacina oliviformis) fruits were harvested and seeds extracted. Five different FYSM samples were prepared. One untreated sample (Un_T) was prepared by crushing fresh false yam seed and sun-drying to 12% moisture. Four treated samples (T) were crushed and each soaked in water (1:2; w/v) for 12 days with water replaced every 3 days. Afterwards, each sample was re-soaked in 1 M concentration of urea (Urea_T), sodium chloride (NaCl_T), sodium hydroxide (NaOH_T) or potassium hydroxide (KOH_T), for 24 h, washed, blanched and sun-dried to a moisture content of 12%. Apparent metabolizable energy (AME) of the experimental diets indicated an increasing trend of AME values as the level of FYSM samples were increased in the diets. Generally, all the treatment diets showed a higher AME content. However, among the treatment diets, urea, potassium hydroxide and sodium hydroxide-treated FYSM-based diets out-performed their sodium chloride treated counterpart (P&lt;0.001) in their AME values. The nutrient metabolisability coefficients of varying inclusion levels of each treated FYSM sample did not influence (P&gt;0.05) the coefficients of metabolisability of dry matter (DM) and crude protein (CP). However, in terms of methods of processing, DM metabolisability was significantly higher (P&lt;0.001) in the FYSM samples that were sequentially treated with water-based and chemical treatment methods than in the untreated sample. Among the sequentially treated FYSM samples, sodium hydroxide-treated samples had the highest (P&lt;0.001) gross energy metabolisability, while the untreated sample recorded the lowest (P&lt;0.001) gross energy metabolisability. The interaction between level of inclusion and treatment methods in this study showed that treatment methods had different effects on crude protein metabolisability (P &lt; 0.011) and gross energy metabolisability (P &lt; 0.017) values of FYSM samples depending on the level of inclusion.

Graded methionine dietary inclusions influence growth performance and apparent ileal amino acid digestibility coefficients and disappearance rates in broiler chickens.

Graded quantities of 1.38, 2.76 and 4.14 g/kg L-methionine were included in a control diet formulated to contain 3.07 g/kg digestible methionine. Each of the 4 dietary treatments was offered to 6 replicate cages (initially 8 birds per cage) from 1 to 21 d post–hatch. The parameters assessed included growth performance, nutrient utilisation (apparent metabolisable energy [AME], AME:GE ratios, N retention, N-corrected apparent metabolisable energy [AMEn]), apparent digestibility coefficients and disappearance rates of amino acids in the distal ileum. They also included free amino concentrations in systemic plasma (brachial vein) at 20 d post–hatch and in hepatic tissue at 14 and 21 d post–hatch. Graded L-methionine inclusions quadratically influenced weight gain (r = 0.688; P = 0.001) and FCR (r = 0.780; P < 0.001). It may be deduced from the quadratic regressions that 3.43 g/kg L-methionine supported maximum weight gain of 1,036 g/kg and 3.50 g/kg L-methionine minimum FCR of 1.193, from 1 to 21 d post–hatch. The control diet contained specified levels of 3.07 g/kg digestible methionine and 13.0 g/kg digestible lysine. Thus, an inclusion of 3.465 g/kg L-methionine corresponded to a total of 6.535 g/kg methionine or a methionine-to-lysine ratio of 50.3, which is higher than standard recommendations. The implications of this and other outcomes of the present study are reported and discussed.

Effects of protease supplementation and diet type on jejunal and ileal digestibility and total tract metabolisability of nitrogen, starch, and energy in broilers

ABSTRACT 1. A study was conducted to assess the effects of supplemental protease (0 or 15,000 units/kg) and diet type (maize- or wheat-based) on apparent jejunal and ileal digestibility and apparent total tract metabolisability of nutrients in Cobb 500 mixed-sex broilers from 6 to 31 d of age. 2. Birds were randomly distributed into 56 metabolism cages (6 birds/cage; 14 replicates/treatment). At 22 d of age, jejunal and ileal digesta contents were collected and pooled from 4 birds/cage to determine apparent digestibility coefficients (DC) and digestible energy (DE). Feed intake was measured, and total excreta were collected from 8 to 11 and 18 to 21 d of age to determine apparent metabolisable energy (AME) and total tract nitrogen and starch metabolisability coefficients. 3. Broilers offered the maize-based diet with protease had greater (P < 0.05) jejunal nitrogen DC, starch DC, and DE (8.2, 6.5, and 14.9%, respectively) and ileal nitrogen DC and DE (5.1 and 6.8%, respectively) than those offered the maize-based diet without protease. Ileal starch DC was increased (P < 0.05) by 1.1% with protease supplementation. 4. Broilers offered maize-based diets had greater (P < 0.05) nitrogen (7.3%) and starch (0.6%) metabolisability coefficients and AME (4.7%) from 8 to 11 d of age, and nitrogen (4.3%) metabolisability coefficients and AME (2.0%) from 18 to 21 d of age compared with those offered wheat-based diets. 5. Thus, protease supplementation and diet type can affect digestive dynamics and nutrient utilisation in broilers.

Nutritive value of expeller/cold-pressed canola meal and pre-pressed solvent-extracted carinata meal for broiler chicken

A study was conducted to evaluate standardized ileal digestibility (SID) of amino acids (AA) and nitrogen-corrected apparent metabolizable energy (AMEn) values of pre-pressed solvent-extracted carinata meal (SE-carinata meal) and expeller/cold-pressed canola meal (ECP-canola meal) for broilers. Two hundred and forty broiler chicks were divided into 40 groups of 6 birds/group and fed 4 diets in a completely randomized design (10 groups/diet) from 14 to 21 d of age. The diets were cornstarch-based containing SE-carinata meal, ECP-canola meal, or pre-pressed solvent-extracted canola meal (SE-canola meal; reference feedstuff) as the sole protein source, and N-free diet. Digestibility of AA and N retention for feedstuffs was determined by the direct method, whereas energy retention of feedstuffs was determined by difference from the N-free diet. On DM basis, SE-canola meal, ECP-canola meal, and SE-carinata meal contained 43, 36, and 50% CP; 2.60, 2.21, and 1.82% Lys; 32, 29, and 27% neutral detergent fiber, and 1.1, 15.3, and 0.88% ether extract, respectively. On DM basis, the AMEn value was lowest (P < 0.05) for SE-carinata meal (1,295 kcal/kg), intermediate (P < 0.05) for SE-canola meal (1,608 kcal/kg), and greatest (P < 0.05) for ECP-canola meal (1,994 kcal/kg). The SID values of indispensable AA for ECP-canola meal were greater (P < 0.05) than those for SE-canola meal or SE-carinata meal. The SID values of all indispensable AA (except Gly, Lys, and Trp) for SE-carinata meal were greater (P < 0.05) than those for SE-canola meal. The SE-canola meal and SE-carinata meal did not differ in SID of Gly and Trp; however, SE-carinata meal had lower (P < 0.05) SID of Lys than SE-canola meal. The results indicate that ECP-canola meal fed in this study could be a good source of AA and energy for broilers. Results also indicate that SE-carinata meal fed in this study could be an attractive AA source for broiler diet, but could benefit from Lys fortification due it its low SID Lys value.

English

The aim of this study was to evaluate the nutritive value of palm kernel cake (PKC) and to investigate its effect on growth performance of broiler chickens. Firstly, the dry matter (DM) digestibility, metabolic energy (ME) of PKC was determined. Secondly, a total of seven hundred 18-day-old Sanhuang broilers were randomly divided into 5 treatments with 7 replicates of 20 broilers each. Broilers were received basal diets without (T0 group) or with 2% (T1 group), 4% (T2 group), 6% (T3 group), 8% (T4 group) PKC supplementation throughout the trial period. The experiment lasted for 18 days after a five days prefeeding period. The growth performance [average daily gain (ADG), average daily feed intake (ADFI), and feed to meat ratio (F/G)] were measured. The results showed that the DM, crude protein (CP), crude fiber (CF), crude fat (EE), crude ash, calcium (Ca), phosphorus (Pi) and gross energy (GE) content of PKC was 91.30%, 15.87%, 14.31%, 10.41%, 3.73%, 0.34%, 0.57% and18.28 MJ/kg respectively. There are 17 kinds of amino acid (AA) in PKC, and had a total AA content of 13.26%. The apparent DM digestibility and true DM digestibility of PKC was 24.11% and 28.73%, respectively. The apparent metabolizable energy (AME) and true metabolizable energy (TME) of PKC was 7.73 MJ/kg and 8.41 MJ/kg, respectively. Dietary PKC supplementation had no effects on the final body weight (FBW), ADG, ADFI and F/G of broilers, while, there was a significant linear relationship between PKC and ADFI (y=0.2006x+73.63, R2=0.8358, p=0.0298). The feed cost were decreased with the increased of PKC supplementation, and the relative economic benefit in T2 group (4% PKC), T3 group (6% PKC), T4 group (8% PKC) was decreased by 1.07%, 0.89%, 0.36%, respectively, only the 2% PKC group increased the relative economic benefit by 0.71% compared with control group. In conclusion, the supplementation of PKC up to 8% in low quality diets did not affects the growth performance of broilers, but considering the economic benefit, it is advisable to add 2% PKC in the diet of broilers

The effect of a direct-fed microbial (PrimaLac) and fat inclusion on performance and energy metabolism in broiler chicks and turkey poults

Two battery trials were conducted to determine the effects of dietary direct-fed microbial (DFM) and dietary fat inclusion on broiler chick and turkey poult performance and dietary apparent metabolizable energy, nitrogen corrected (AMEn) when fed corn, wheat, and soy diets. For both experiments, a 2 x 2 factorial experimental design was used with supplemental fat at low (1%, LF) or high (6%, HF) levels and DFM inclusion (0 or 0.91 kg/ton) as main effects. Dietary treatments were randomly assigned to 72 cages of birds and fed for 21 days. Growth performance was measured weekly, and cecal contents were collected for volatile fatty acid (VFA) analysis on day 21. Fat supplementation resulted in increased body weight gain (BWG) in both broilers and turkeys. Specifically, in LF diets, DFM inclusion resulted in increased BWG compared to the non-DFM treatments. Cumulative feed conversion ratio (FCR) was improved in HF treatments and the LF with DFM treatment compared to the LF with no DFM. In both trials, as expected, feeding HF diets resulted in increased AMEn. However, the DFM inclusion resulted in a greater uplift in AMEn in LF diets for both broilers and turkeys. In either trial, diet did not impact cecal VFA concentrations; thus, the impact on DFM on VFA production remains uncertain. In conclusion, the performance of broiler chicks and turkey poults was improved by DFM inclusion in reduced fat diets which was associated with increased energy digestibility as measured by AMEn.

A study on citric acid by-product as an energy source for Japanese quail.

The increasing worldwide production of citric acid by the fermentation of substrates for use as a flavoring and a preservative in foods has resulted in the generation of large amounts of waste and by-products from fermented. There is a challenge to reduce the waste from these products by using them as animal feed. An experiment was conducted to study the utilization of citric acid by-products (CABP) as a feed ingredient in Japanese quail diets. A total of 560 1-day-old quail chicks were randomly distributed into five groups, with CABP included at 0, 3, 6, 9, and 12% of the diets. Each treatment had four replicate pens of 28 birds per pen throughout the 42-day trial period. CABP inclusion at 9 or 12% of the diet, respectively, resulted in a decrease in feed intake of 5.90 and 9.52%, body weight of 5.67 and 9.16%, and body weight gain of 5.44 and 9.97%, compared with the control diet (p < 0.05). Carcass traits and relative organ weights were not significantly different among the treatments (p > 0.05). The 12% CABP group showed a decrease in the digestibility of crude protein and apparent metabolizable energy, but there was an increase in the amount of crude fiber compared with the control diet (p < 0.05). The utilization of CABP in diets can reduce feed costs leading to increased investment returns. In conclusion, CABP can be included at up to 6% of quail diets without significant effects on growth performance or nutrient digestibility.

Significance of single β-mannanase supplementation on performance and energy utilization in broiler chickens, laying hens, turkeys, sows, and nursery-finish pigs: a meta-analysis and systematic review.

This review will give a brief description of β-mannans, abundance in feedstuffs, utility of supplemental feed β-mannanase, and subsequent animal responses. Soybean products and co-products of processing palm, coconut, and guar seeds are the major sources of β-mannans in poultry and livestock feed. β-Mannans are linear polymers of mannose residues linked by β-1,4 glycosidic bonds and their ingestion elicit undesirable and metabolically costly responses. Web of Science was searched to retrieve published studies for meta-analyses of the impact of supplemental β-mannanase on performance and digestibility in pigs and poultry. The mean difference (MD) between β-mannanase and control on average daily gain (g/d) was +0.23 (P = 0.013; 95% CI of 0.05; 0.41), +10.8 g/d (P = 0.0005; 95% CI of 6.6; 15.0 g/d), and +20.68 (P < 0.000; 95% CI of 17.15; 24.20 g/d) for broiler chickens, nursery pigs, and grow-finish pigs, respectively. The MD on β-mannanase improvement on feed conversion (FCR) was −0.02 (P < 0.0001) with 95% CI (−0.03; −0.02) suggesting a 2-to-3-point FCR improvement in broiler chickens. β-Mannanase improvement on gain to feed (G:F) was +13.8 g/kg (P = 0.027; 2.1; 25.4 g/kg) and +8.77 g/kg (6.32; 11.23 g/kg) in nursery and grow-finish pigs, respectively. β-Mannanase improved apparent metabolizable energy by 47 kcal/kg (P = 0.0004) with 95% CI (28.8; 65.7 kcal/kg) in broiler chickens. The improvement of gross energy digestibility in pigs was 1.08% unit with 95% CI (0.90; 1.26) translating to the release of between 30.6 and 42.8 kcal/kg of digestible energy. Although data were limited, β-mannanase improved egg production in laying hens linked to improved energy metabolism in laying hens linked to improved energy metabolism but had no impact on egg quality. Turkeys may be more adversely affected by β-mannans because of the high protein/amino acids requirements necessitating higher dietary inclusion of soybean meal. However, growth performance and feed efficiency responses of turkeys fed diets supplemented with β-mannanase were variable. In summary, β-mannanase supplementation improved performance linked to energy and nutrient utilization. However, the magnitude of response was variable within and between species indicating further application refinement is warranted to achieve consistent efficacy, and improved understanding of the functional contribution of β-mannans hydrolysis products.

Influence of Trans-anethole on the nutrient digestibility and intestinal barrier function in broilers

This experiment was undertaken to investigate the effects of dietary trans-anethole (TA) at 5 levels (0, 200, 400, 600, and 800 mg/kg of diet) on the growth performance, apparent nutrient digestibility and intestinal barrier function in broilers. Three hundred twenty 1-day-old Arbor Acres broilers were randomly divided into the 5 dietary treatments with 8 replicates each for 42 d. Dietary TA supplementation increased (P < 0.05) average daily feed intake (ADFI), but had no effects (P > 0.05) on average daily gain (ADG), feed/gain (F/G), and body weight (BW) of broilers throughout the entire experimental period. The apparent metabolizable energy (AME) and nitrogen-corrected apparent metabolizable energy (AMEn), the apparent total tract digestibility of dry matter (DM), crude protein (CP), organic matter (OM), and gross energy (GE) showed a quadratic increase (P < 0.05) with the increasing TA concentration in the diet. The apparent ileal digestibility of Lys, Met, Leu, Thr, Ala, Tyr, and Pro were higher (P < 0.05) in birds fed TA diets compared with control group. Dietary supplementation of 400 mg/kg of TA increased (P < 0.05) mRNA levels of jejunal and ileal Na+/glucose co-transporter (SGLT1) on d 21 and d 42, oligopeptide transporter 1 (PepT1) on d 42, and ileal mRNA expressions of occludin (OCLN), claudin-1 (CLDN-1), and mucin 2 (MUC2), villus height (VH), crypt depth (CD), and VH:CD on d 21, as well as jejunal zonula-occludens-1 (ZO-1) and ileal mucin 2 on d 42. Linear or quadratic responses of the jejunal CD and villus VH:CD ratio occurred (P < 0.01) with increasing dietary TA concentration on d 42. The inclusion of 400 mg/kg TA decreased (P < 0.05) cecal Escherichia coli population on d 21 and d 42, but increased (P < 0.05) Bifidobacterium population on d 21 and ileal Bifidobacterium on d 42. In conclusion, 400 mg/kg of TA is the optimum concentration for increasing nutrient utilization and intestinal barrier function of broilers.