Apparent Digestibility Of Organic Matter Research Articles

Pimenta como aditivo fitogênico em dietas para ovinos em terminação

ABSTRACT The objective of this study was to evaluate the effect of a phytogenic additive on nutrient intake, diet digestibility, nitrogen balance, and ruminal parameters of lambs. Four lambs with an average body weight of 27.6 ± 2.9 kg were distributed in a 4 × 4 Latin square design and fed a basal diet composed of 600 g DM/day of corn silage and 400 g DM/day of concentrate. The basal diet was supplemented with no additive 0.0, 2.0, 4.0, and 6.0 g/kg DM of pepper. The intake of DM, crude protein (CP), organic matter (OM), and neutral detergent fiber (NDF) in g/day decreased linearly (P < 0.05). No effects (P > 0.05) were observed for the apparent digestibility of DM, OM, NDF, and ether extract. There was a quadratic effect (P < 0.05) on the digestibility of CP and non-fibrous carbohydrate, with maximum digestibility estimated at 707.7 and 924.8 (g/kg DM) at levels of 2.55 and 0.27 g/kg DM of pepper, respectively. There was no effect (P > 0.05) on urinary nitrogen and retention nitrogen. Increasing levels of pepper did not alter (P > 0.05) the pH value and N-NH3 concentration of the ruminal fluid. However, there was an effect (P < 0.05) of time on the pH and N-NH3 value of the rumen. The inclusion of pepper in the diet of confined lambs negatively alters dry matter and NDF intake without impairing nutrient digestibility, nitrogen utilization, and other ruminal parameters.

Effects of Spent Substrate of Oyster Mushroom (Pleurotus ostreatus) on Feed Utilization and Liver Serum Indices of Hu Sheep from the Perspective of Duodenal Microorganisms.

This study aimed to evaluate the effects of Pleurotus ostreatus spent mushroom substrate (P.SMS), which is characterized by high production but low utilization, on feed utilization and liver serum indices from the perspective of duodenal microorganisms. Forty-five 3-month-old Hu sheep were randomly assigned to five groups and fed diets in which whole-plant corn silage (WPCS) was substituted with P.SMS at levels of 0% (Con), 5% (PSMS5), 10% (PSMS10), 15% (PSMS15), or 20% (PSMS20). The results indicated that the addition of P.SMS complexly influenced the apparent digestibility of dry matter, organic matter, and crude protein, with PSMS10 showing the highest digestibility of these nutrients. P.SMS inclusion significantly affected serum alanine aminotransferase levels, with PSMS5 showing higher levels than both the Con and PSMS20 groups (p < 0.05). Importantly, the inclusion of P.SMS did not affect the richness and diversity of duodenal microorganisms. Significant differences in the phyla Verrucomicrobiota and Spirochaetota were observed between the Con and PSMS20 groups. The observed trend towards an increase in the genus Trichoderma (p = 0.057) suggests that P.SMS is susceptible to contamination by this genus, which in turn affects the structure of the intestinal flora. Furthermore, functional gene predictions indicated differences in amino acid metabolism among the groups (p < 0.05). In conclusion, feeding with 10% P.SMS resulted in the highest digestibility without adversely affecting the structure of the duodenal community or liver function.

Limpograss [Hemarthria altissima] Silage and Protein Supplementation as an Alternative Feed Option for Growing Heifers in North Florida.

Limpograss (Hemarthria altissima) is a warm-season perennial grass that has the potential to feed livestock during scarcity periods. This study evaluated the intake, nutrient digestibility, and animal performance of beef heifers fed 'Gibtuck' limpograss silage combined with different levels of a range cube supplementation. Twenty-four heifers (330 ± 16 kg live weight) were submitted to four different treatments with 6 replicates: (1) control, no supplementation + limpograss silage ad libitum; (2) 1.4 kg of supplement + limpograss silage ad libitum; (3) 2.8 kg of supplement + limpograss silage ad libitum; and (4) 4.2 kg of supplement + limpograss silage ad libitum. The apparent total tract digestibility of dry matter, organic matter, and crude protein showed a positive quadratic effect with increasing supplementation levels (p = 0.001, p = 0.002, and p < 0.0001, respectively). Overall, the supplement improved diet digestibility and total DM intake but reduced silage intake, indicating a combined effect (substitutive and additive effect) of the protein supplement. The increasing level of protein supplement increased the average daily gain with a quadratic effect (p ≤ 0.0001). Limpograss silage associated with supplementation can improve diet digestibility and increase the animal performance of growing heifers, providing an alternative for livestock in North Florida.

Effect of Postbiotic Supplementation on Nutrient Digestibility and Milk Yield during the Transition Period in Dairy Cows.

The metabolites secreted by probiotics or released after their lysis are called postbiotics. They provide physiological benefits to the host, preventing the colonisation of pathogens by improving the intestinal environment for beneficial commensal bacteria, which reduces the incidence of digestive disorders and improves the immune system. The aim of this work was to evaluate the addition of postbiotics to dairy cow rations during the transition period on nutrient digestibility, composition, and milk yield. The effects of two postbiotics were evaluated in twelve Friesian cows from 30 days before calving to two months of lactation. The animals were randomly allocated to two treatment groups: control (CT) and supplemented with postbiotics (PC and PR). Feeding was ad libitum with 60/40 of forage/concentrate ratio on dry matter basis. Daily feed intake and milk production were recorded individually throughout the study. Two digestibility balances were performed, one before parturition and one after parturition. Colostrum was sampled at first milking and milk was sampled weekly. Data were analysed using a mixed mode in R software 4.4.1. The results suggest that postbiotic supplementation in late gestation and early lactation increase the voluntary intake of dry matter, especially in the PR treatment, with higher apparent total tract digestibility of dry matter, organic matter and neutral detergent fibre. Both treatments including postbiotics induced an increase in colostral immunoglobulin concentration. Milk production of cows receiving the PC treatment was the highest, with high fat and protein yields and a higher persistence of the production curve throughout the lactation.

Gestating sows with a restricted feed allowance require a longer adaptation period before collecting feces compared with lactating sows in total tract digestibility assessments using index method.

The objective of the present experiment was to determine the minimum adaptation period for total tract digestibility experiments in gestating and lactating sows using the indigestible index method. Five gestating and 5 lactating sows at parities 3 to 5 were used. An indigestible index of 0.5% chromic oxide was supplemented to a diet based on corn and soybean meal. The daily feed allowance for gestating sows was 2kg and 2 equal meals were provided to the sows. Lactating sows were fed 6kg of feed per day in 3 equal meals. After feeding a diet without supplemental chromic oxide for 5 d, index-supplemented diets were provided to the gestating and lactating sows. Feces were collected at 24-h intervals for 9 and 7 d from gestating and lactating sows, respectively. Fecal Cr concentrations increased linearly (P < 0.001) and quadratically (P < 0.001) with collection time in both gestating and lactating sows. Minimum adaptation periods were estimated by one-slope broken-line model. The break point of Cr concentrations in feces was day 7.2 (SE = 0.3) in the gestating sows and day 4.2 (SE = 0.2) in the lactating sows, respectively. Apparent total tract digestibility of dry matter, organic matter, and energy on day 4 was less (P < 0.001) than that on days 8 to 9 in gestating sows fed the experimental diet with a 2-kg feed allowance. In lactating sows fed the experimental diets with a 6-kg feed allowance, the apparent total tract digestibility of dry matter, organic matter, and energy on day 3 was less (P < 0.05) than that on days 5 to 7. In conclusion, at least 8 d of adaptation period are required for gestating sows to determine total tract digestibility using Cr as the indigestible index method whereas 5 d of adaptation period are required for lactating sows. An insufficient adaptation period results in lower digestibility values.

Feeding milk thistle (Silybum mariamum) seed powder to fattening lambs: Effects on growth performance, digestibility and rumen metabolism

This experiment was conducted to evaluate the effect of milk thistle (Silybum marianum) seed powder supplementation in the diet on weight gain, feed conversion efficiency, and physiological parameters in fattening lambs. In this experiment, thirty crossbred Shawl × Romanov male lambs were divided into three groups based on a completely randomized design. The experimental treatments included a control group receiving a diet without milk thistle seed powder (MSP), a group receiving a diet containing 1 % MSP, and a group receiving a diet containing 2 % MSP. Animals fed the diet containing 2 % MSP exhibited higher nutrient intake, average daily gain, and final body weight compared to the other treatment groups (P < 0.05). Lambs receiving the 2 % MSP diet had the most favorable feed conversion ratio (P < 0.05). The apparent digestibility of dry matter, organic matter, and crude protein was highest in lambs fed the diet containing MSP compared to the control group (P < 0.05). Ruminal NH3-N concentration and populations of total protozoa and Entodinium genus were lowest in lambs fed the MSP-containing diets (P < 0.05). The molar concentrations of acetate and valerate in the rumen fluid of lambs receiving the diet containing 2 % MSP were lower compared to the control group, while propionate concentration was higher (P < 0.05). Feeding the diet containing 2 % MSP reduced serum cholesterol, triglycerides, LDL, and liver enzyme activities compared to the control group (P < 0.05). Based on these results, supplementing lamb diets with 2 % MSP increased growth performance measures, nutrient digestibility coefficients, and reduced blood cholesterol and LDL concentrations.

Effects of different concentrate levels in AGRI-002E sorghum silage-based diets on nutrient intake and digestibility, ruminal pH and ammonia concentration, ruminal degradability, and microbial efficiency in beef cattle

This study aimed to evaluate the effect of increasing concentrate levels in AGRI-002E sorghum silage (SS)-based diets on nutrient intake and digestibility, ruminal pH and ammonia concentration, Nitrogen (N) balance, efficiency of microbial protein synthesis, and in situ degradability of complete diets. Five rumen-cannulated Nellore bulls (age = 8 ± 1.0 months; initial BW = 242 ± 5 kg) received five dietary treatments in a 5×5 Latin square experimental design. The dietary treatments consisted of five concentrate levels (0, 200, 400, 600, and 800 g of concentrate/kg on a DM basis) in SS-based diets. The experiment lasted 120 d, with five periods of 24 d. Each period consisted of 17 d for dietary adaptation, and 7 d for data collection. In situ degradability assays were conducted to estimate ruminal degradability. Total feces and urine collection were performed to estimate nutrient intake and digestibility and estimate N balance. Omasal and ruminal digesta collection were performed to estimate ruminal digestibility and ruminal parameters. Increasing concentrate levels in SS-based diets led to linear increases (P<0.001) in the intake of dry matter (DM), organic matter (OM), crude protein (CP), and ether extract (EE). Neutral detergent fiber corrected for ash and protein contamination (apNDF) and starch intake showed quadratic responses (P≤0.008). Ruminal digestibility of DM, OM and CP responded quadratically (P≤0.040), while apNDF exhibited linear decrease (P=0.003). Starch ruminal digestibility exhibited a cubic effect (P=0.016). Apparent total-tract digestibility of DM, OM, and EE increased linearly (P≤0.001), whereas apNDF, and CP digestibility decreased linearly (P≤0.012), and starch presented a quadratic effect (P=0.029). In situ ruminal degradation parameters increased linearly with higher concentrate levels (P<0.001). Ruminal pH exhibited a quadratic pattern (P=0.006), ammonia concentration linearly decreased (P=0.003). Total volatile fatty acids, and butyrate showed linear increases (P<0.001), acetate and propionate had quadratic effects (P<0.001), while the ratio Acetate to Propionate decreased linearly (P<0.001). N intake, fecal N excretion, retained N, total digestible nutrients, digestible organic matter, and microbial production increased linearly (P<0.001), although urine N excretion, urinary urea, and blood urea concentration decreased linearly (P<0.028). Therefore, increasing concentrate levels in AGRI-002E sorghum silage-based diets improve TDN intake, microbial protein synthesis efficiency, in situ ruminal degradability parameters, and nitrogen utilization. Moreover, our findings indicate that AGRI-002E sorghum silage demonstrates potential as a fiber source for high-concentrate diets. However, its effectiveness is limited without concentrate supplementation, emphasizing the importance of balanced dietary composition for optimal utilization in beef cattle.

Evaluation of Effective Energy Values of Six Protein Ingredients Fed to Beagles and Predictive Energy Equations for Protein Feedstuff.

This study evaluated the nutrition composition, the nutrient digestibility, and the energy value of six protein ingredients used in pet food by the difference method in six beagles within a 7 × 6 incomplete Latin square design. The results showed that the apparent total tract digestibility of gross energy (GE) and organic matter (OM) in beagles fed the fish meal (FM) and corn gluten meal (CGM) diets was higher than for those fed the meat and bone meal (MBM), soybean meal (SBM), mealworm meal (MM), and yeast extract (YE) diets (p < 0.05). The digestible energy (DE), metabolizable energy (ME), and net energy (NE) of the MM diet were greater than the other diets, and MBM was the lowest (p < 0.05). The ME of protein ingredients was positively correlated with organic matter and negatively correlated with the ash content. The NE of protein ingredients was positively correlated with the crude protein content and negatively correlated with the ash content. The study resulted in predictive energy equations for protein ingredients that were more accurate than the NRC's predictive equation of ME when the ash content of the ingredient was more than 30% DM. In conclusion, the nutrient digestibility and energy value of corn gluten meal were similar to those of fish meal and those of soybean meal were similar to yeast extract. All predictive energy equations for six protein feedstuffs had slight differences with measured energy values.

Interactive Effects of Dietary Fiber and Lipid Types Modulate the Predicted Production and Absorption of Cecal and Colorectal Short-Chain Fatty Acids in Growing Pigs

BackgroundHigh-fiber diets are supplemented with lipids to meet the required energy content, but data on the interactions between dietary fiber (DF) and lipid types on gastrointestinal fermentation in pigs are scant. ObjectivesThis study aimed to use a combination of in vivo and in vitro fermentation methodologies to determine the interactive effects of DF and lipid types on short-chain fatty acid (SCFA) production and absorption and organic matter (OM) fermentability in the cecum and colorectal tract of pigs. MethodsEight ileal- and cecal-cannulated Yorkshire barrows were fed either pectin- or cellulose-containing diets that were supplemented with either corn oil or beef tallow in 2 independent Youden squares with a 2 × 2 factorial arrangement of treatments (n = 6). Ileal and cecal digesta were collected, freeze-dried, and fermented using inoculum from fresh cecal digesta and feces, respectively, to determine individual SCFA production and absorption and fermentability of OM. ResultsInteractions (P < 0.001) between DF and lipid types were observed in which the addition of beef tallow decreased the quantity of cecal and colorectal acetic acid production and cecal acetic absorption, cecal butyric production, predicted cecal OM fermentability, and predicted colorectal propionic acid in pectin diets, but the effects were not observed for cellulose diets. The addition of beef tallow increased (P < 0.001) the production of cecal butyric and propionic acids during in vitro fermentation in cellulose diets and apparent total tract digestibility (ATTD) of OM in pectin diets. ConclusionsThe interactions between DF and lipids on gastrointestinal fermentation largely depend on the degree of saturation of fatty acids in dietary lipids. The addition of beef tallow selectively decreased the production and absorption of individual SCFAs in pectin and cellulose diets but increased cecal butyric and propionic acid production in cellulose diets and the ATTD of OM in pectin diets.

PSVI-5 Extruded blend of Nannochloropsis oculata microalgae and flaxseed and its effects on ruminal fermentation and nutrient digestion

Abstract Omega-3 fatty acids are essential nutrients for mammals, and are required for cellular growth and synthesis of numerous cellular signaling molecules. NBO3 Technologies, LLC (Manhattan, KS) has developed an omega-3 supplement consisting of an extruded blend of flaxseed and Nannochloropsis oculata microalgae (GreatOplus; GOP). Our objective was to evaluate effects of GOP on dry matter intake (DMI), water intake (WI), ruminal metabolism, and apparent total tract nutrient disappearance in cannulated Holstein steers (n = 11; initial body weight 586.4 ± 49.7 kg). Treatments consisted of backgrounding diets (40% roughage, 60% concentrate) containing 0% (CON) or 10% GOP (dry basis). Feed and water intake were recorded using an Insentec Roughage and Water monitoring system (Hokofarm Group; Emmeloord, The Netherlands). Steers were utilized in a cross-over design with two 19-d periods. Each period consisted of a 15-d adaptation phase and a 4-d collection phase. Ruminal contents, duodenal digesta, and feces were collected at 8-h intervals for 4 d starting at 1000 h on d 16. The sampling schedule was advanced by 2 h with each subsequent sampling day, thus generating a 24-h profile at 2-h intervals post-feeding over the 4-d sampling phase. Titanium dioxide (15 g) was dispensed directly into the rumen of each steer 15 min before feeding and to estimate apparent ruminal flow and fecal excretion of nutrients. There was a Treatment X Day interaction for DMI (P &amp;lt; 0.001); DMI was greater for steers fed GOP some of the days during the adaptation phase compared with steers fed the control diet, but intakes were similar during the collection phase. Ruminal fluid pH, ruminal ammonia concentration, and WI were not affected by treatment (P &amp;gt; 0.10). As expected, ruminal outflows of total fatty acids (195.6 vs 338.1 g/d for CON and GOP, respectively) and α-linolenic acid (ALA; 1.6 vs 6.3 g/d, respectively) were greater for steers fed GOP compared with steers fed CON (P &amp;lt; 0.05); however, ruminal disappearance (i.e., biohydrogenation) of ALA was extensive, with only 8% and 4.7% of ALA intake exiting the rumen for CON and GOP, respectively. Apparent total tract digestion of organic matter was not impacted by diet (P &amp;gt; 0.20; 78.1 and 76.8% for CON and GOP, respectively). Supplementing an extruded blend of flaxseed and microalgae can effectively increase the amount of α-linolenic acid available for absorption in the small intestine, but overall recovery of dietary α-linolenic acid is relatively low due to extensive biohydrogenation by microbes within the rumen.

Isobutyramide and Slow-Release Urea as Substitutes for Soybean Meal in the Finishing Diet of Beef Cattle.

Two experiments were conducted to investigate the effects of isobutyramide (IBA) and slow-release urea (SRU) as substitutes for soybean meal (SBM) in the finishing diet of beef cattle. The completely randomized design in vitro experiment with five treatments, i.e., control, 0.9% SRU group, 0.6% SRU + 0.3% IBA group (SRU-I), 0.3% SRU + 0.6% IBA group (IBA-S), 0.9% IBA group was conducted. The results showed that the IBA-S and IBA increased (p ≤ 0.05) substrate disappearance of dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), total gas, and total volatile fatty acids (TVFA). The SRU group had the highest (p < 0.01) crude protein disappearance and ammonia nitrogen concentration, but the IBA contrarily decreased (p < 0.01) them compared with the control. Inclusion of IBA increased isobutyrate concentrations (p = 0.01) with the highest value for the IBA group. Then, an 84-day replicate 4 × 4 Latin square design with 8 Angus steers and four treatments, i.e., control, SRU, SRU-I, IBA-S was performed. The results showed that the treatments did not affect DM intake (p > 0.05) but tended (p = 0.09) to increase average daily gain. The inclusion of IBA increased (p < 0.05) the apparent digestibility of DM, organic matter, NDF, ADF, TVFA, and microbial crude protein with the highest values for the IBA-S group. The IBA-contained groups also increased (p ≤ 0.01) isobutyrate concentration, activities of carboxymethyl cellulase and xylanase, and the relative abundance of Butyrivibrio fibrisolvens with the highest values for the IBA-S group. The SRU had no effect on animal growth and nutrient apparent digestibility. In conclusion, IBA was developed as a new substitute for SBM in the finishing diet of beef cattle, and the optimal strategy was the isonitrogenous substitution of SBM with 0.3% SRU and 0.6% IBA of the diet.

Energy metabolism and respirometry of lambs fed sun or shade dried hays of banana crop residues.

We aimed to evaluate the effects of different drying methods for banana residues on the energy metabolism and respirometry of growing lambs. Twenty Santa Inês x Dorper lambs were fed five experimental diets: Tifton 85 grass hay (Control), shade-dried banana leaf hay (LH Shade), shade-dried pseudostem banana hay (PH Shade), sun-dried banana leaf hay (LH Sun), and sun-dried banana pseudostem hay (PH Sun). Nutrient intake and digestibility were assessed in metabolic cages, whereas O2 consumption and CO2, methane, and heat production were measured in a respirometry chamber with animals fed at maintenance and ad libitum levels. Nutrient and energy intake was not influenced by diet. Pseudostem hay had higher apparent digestibility of dry matter (71.5%), organic matter (72.4%), and neutral detergent fiber (58.0%). However, this led to greater energy loss in the form of methane (12.1%). The banana residue hays and drying methods did not alter oxygen consumption, CO2 production, or heat production of animals fed ad libitum or during maintenance. On the other hand, the use of leaf hay resulted in a reduction of 24.7% in enteric methane production of animals fed ad libitum. The inclusion of pseudostem hay is recommended in sheep feedlot diet. This residue provided greater use of DM, however promoted a greater loss of energy in the form of methane, resulting in similar energy consumption. The drying methods did not reduce the availability of nutrients and the sun drying method is recommended, since it is a faster drying method.

Nutritional, ruminal, and metabolic parameters of beef bulls fed high-energy diets as a function of dietary addition of a magnesium oxide blend associated or not with monensin

This study aimed to evaluate the impact of a magnesium oxide blend (MG) and monensin (MON) on the nutritional, ruminal, and metabolic parameters of finishing bulls fed high grain diet. Six ruminally cannulated bulls (542 ± 33.6 kg) were used in a 6 × 6 Latin square design with treatments arranged as a 2 × 3 factorial, with 6 periods of 23 days. The MG was included at 0, 2.5, or 5.0 g/kg of dry matter (DM) - MG0, MG2.5, and MG5.0, respectively, without or with MON (30 mg/kg of DM). During each experimental period, days 1–10 were designated for dietary additive adaptation. Days 11–20 (feeding period), were used to assess DM and nutrient intakes, digestibility, ruminal pH, volatile fatty acids (VFA) profile, and blood parameters. Days 21–23 (corn challenge period), included supplementing all treatments with 2 kg/day of ground corn, replacing 2 kg/day of forage, to observe responses in ruminal pH and DM intake (DMI) in the presence of increased fermentable carbohydrates in the rumen. The ruminal pH increased linearly (P = 0.01) with the MG level during the feeding period. In the corn challenge, bulls fed diets without MON and MG5.0, as well as those fed MON and MG2.5 or MG5.0 showed a marked increase in rumen pH value during the first day. In the feeding period, the DMI was greater (quadratic: P = 0.05) for MG2.5 compared to other tested doses. The DM and nutrient intakes were greater (quadratic: P ≤ 0.05) for the MG2.5 group. Bulls fed MON diets had 0.5 kg lower DM and nutrient intakes than those without MON (P ≤ 0.05). The ruminal and intestinal digestibility of diet compounds did not differ among treatments (P ≥ 0.08). The DM and organic matter (OM) apparent total-tract digestibility tended to be (quadratic: P = 0.06) and were greater (quadratic: P = 0.05) for the MG2.5 group. The microbial crude protein synthesis (MCP) was greater for bulls fed the MG2.5 diet (quadratic: P = 0.03). Bulls fed diets without MON had a lower valeric acid (MON × Time: P = 0.03) and those fed diets without MG had a lower iso-valeric acid (MG × Time: P = 0.03) molar proportion 12 hours post-feeding than 1 hour pre-feeding. Glucose levels increased linearly with MG doses (P < 0.001). In summary, a dosage of 5.0 g/kg of DM of MG promotes a superior response in ruminal pH, whereas beneficial effects on digestive parameters are achieved with 2.5 g/kg of DM. Furthermore, MON and MG showed no significant associative effects.

Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits.

Sixteen 35-day-old male crossbred rabbits (New Zealand white × Thai native breed) with an initial weight of 484 ± 11.3 g were randomly divided into two groups of eight, constituting control and treatment groups. The treatment group was orally administered a crude extract of butterfly pea (Clitoria ternatea L.) at 0.5 g/kg body weight from weaning (at 35 days) to slaughter (at 90 days). The effects on the phagocytic activity of blood polymorphonuclear leukocytes, serum biochemistry, meat quality, muscular lipid peroxidation, the apparent digestibility of dry matter and nutrients, and gut histology were studied. The results revealed that the phagocytic function of circulating leukocytes (75 and 90 days) and alveolar macrophages (90 days) did not differ between the two groups. At slaughter, treated rabbits had lower blood urea nitrogen concentrations and higher liver weight than control rabbits (p < 0.05). After chilling at 4 °C for 24 h, a lower meat pH and the alteration of meat color (brighter, less yellow, lower hue angle, and decreased color saturation) were observed in the treated group (p < 0.05). Furthermore, lipid peroxidation (measured at 3, 5, and 7 storage days) in the meat of treated rabbits was lower than in controls (p < 0.05). The apparent digestibility of organic matter and ether extract (analyzed at 46 days for 4 days) was improved in the treated group (p < 0.05), whereas gut histology was unaffected. In conclusion, butterfly pea extract supplementation did not affect phagocytic function but led to a modification in meat color, delayed lipid peroxidation, and improved digestibility.

Replacing conventional concentrates with sprouted barley or wheat: Effects on lactational performance, nutrient digestibility, and milk fatty acid profile in dairy cows

Finite natural resources, rising human population, and climate change pose challenges to traditional crop production. Hydroponically grown fodder (i.e., sprouted grains) can be an alternative feed source for dairy cows; however, only sprouted barley has been investigated in low-producing cows. We aimed to evaluate the effect of replacing conventional concentrates with sprouted barley or wheat, grown using hydroponics, on milk production, nutrient digestibility, and milk fatty acid profile in high-producing cows. Twenty-four multiparous Holstein cows (3.25 ± 1.33 lactations; 102 ± 23 DIM; 49 ± 4 kg/d of milk) were used in a replicated 3 × 3 Latin square design with 21-d experimental periods. Following a 2-wk covariate period, cows were fed 1 of 3 experimental diets: a TMR (1) without sprouted grains (control), or with (2) 10% sprouted barley, or (3) 10% sprouted wheat on a DM basis. Experimental diets were formulated to be isoenergetic and isonitrogenous with sprouted grains that replaced ground corn, soybean meal, canola meal, and dextrose. Sprouted grains were grown using a semi-automatic hydroponic system and harvested after 6 d of growth. Data and sample collection occurred during the last 3 d of the covariate and experimental periods. Wide ranges were observed for the DM percent of sprouted grains (12.1%-22.9% and 13.3%-25.7% for barley and wheat, respectively) and the ratio of sprouted fodder to seed (0.67-1.07 for both barley and wheat). Feeding sprouted grains did not modify the yield of milk or ECM; however, DMI were lower for barley, relative to control. Feed efficiencies were greater for barley than for control (1.49 ± 0.03 vs. 1.43 ± 0.03 for milk yield/DMI; 1.85 ± 0.03 vs. 1.73 ± 0.04 for ECM/DMI). Yields and concentrations of milk components (i.e., fat, true protein, and lactose) were not affected by treatment. Milk urea N concentrations were greater for wheat, relative to control or barley. Body weight (752 ± 3 vs. 742 ± 3 kg) and BW gains (6.53 ± 2.99 vs. -9.33 ± 2.91 kg/21 d) were higher for wheat than for control. Apparent total-tract digestibility of organic matter was greater for wheat relative to barley. Digestibilities of NDF and starch were higher for wheat and control, relative to barley, and CP digestibility was greater for wheat, relative to barley and control. Rumination and physical activity were not affected by treatment. In summary, replacing traditional concentrates with sprouted grains grown using hydroponics improved milk production efficiency (barley sprouts) or enhanced body weight gain (wheat sprouts). A life-cycle assessment needs to be conducted to determine the net effect of this feeding strategy for the dairy industry.

The Effects of Harvest Maturity of Eragrostis tef 'Moxie' Hay and Supplemental Energy Source on Forage Utilization in Beef Heifers.

The phenological stage of maturity of grasses and supplementation program can impact forage utilization in grazing beef cattle. However, the potential interaction between harvest maturity of Eragrostis tef (teff) hay and energy supplement source was yet to be fully evaluated. Therefore, our objective was to determine the effects of harvest maturity of teff hay and supplemental energy sources on nutrient intake, apparent total-tract nutrient digestion, nitrogen (N) utilization, and ruminal fermentation characteristics in beef heifers. A split-plot design with teff hay harvest maturity as the whole plot and supplemental energy source as the subplot was administered in a three-period (21 d), three × three Latin square design. Six crossbred beef heifers (804 ± 53.6 kg of body weight; BW) were allocated to two harvest maturities (early- (EH]) or late-heading (LH)) and to two supplemental energy sources (no supplement (CON), or rolled corn grain or beet pulp pellet fed at 0.5% of BW). Data were analyzed using SAS. There was no harvest maturity × energy supplement interaction. Although harvest maturity had no impact on total dry matter intake (DMI), crude protein (CP) intake was greater (p < 0.01) for EH than LH heifers. Total intakes of dry (DM) and organic matter (OM) were also greater (p < 0.01) for supplemented than CON heifers, whereas acid detergent fiber (ADF) intake was greater for beet pulp heifers compared to heifers fed the CON diet and supplemental corn grain. Harvest maturity had no impact on ruminal pH. However, mean ruminal pH was lower (p = 0.04), duration pH < 6.2, and molar proportions of butyrate and branched-chain fatty acids were greater (p ≤ 0.049) for heifers fed corn grain compared to CON and beet pulp diets. Heifers fed EH hay had greater (p ≤ 0.02) apparent total-tract DM, OM, CP, NDF, and ADF digestibility than heifers fed LH hay. Although there was no supplemental energy effect on microbial nitrogen (N) flow, it was greater (p < 0.01) for EH than LH heifers. Apparent N retention, which did not differ, was negative across all diets. In summary, delaying the harvest of teff hay from the EH to LH stage of maturity compromised nutrient supply, which was not attenuated by feeding supplemental corn grain and beet pulp at 0.5% of diet DM. Because N retention was negative across harvest maturity, there might be a need to provide both energy and protein supplements to improve growth performance when feeding teff hay to beef cattle.

Reducing dietary protein and supplementation with starch or rumen-protected methionine and its effect on performance and nitrogen efficiency in dairy cows fed a red clover and grass silage–based diet

The increasing cost of milk production, in association with tighter manure N application regulations and challenges associated with ammonia emissions in many countries, has increased interest in feeding lower crude protein (CP) diets based on legume silages. Most studies have focused on alfalfa silage, and little information is available on low-CP diets based on red clover silage. Our objectives were to examine the effects of dietary CP content and supplementing a low-CP diet with dietary starch or rumen-protected Met (RPMet) on the performance, metabolism, and nitrogen use efficiency (NUE; milk N output/N intake) in dairy cows fed a red clover and grass silage-based diet. A total of 56 Holstein-Friesian dairy cows were blocked and randomly allocated to 1 of 4 diets over a 14-wk feeding period. Diets were based on red clover and grass silages at a ratio of 50:50 on a dry matter (DM) basis and were fed as a total mixed ration, with a 53:47 ratio of forage to concentrate (DM basis). The diets were formulated to supply a similar metabolizable protein (MP) content, and had a CP concentration of either 175 g/kg DM (control [CON]) or 150 g/kg DM (low-protein [LP]), or LP supplemented with either additional barley as a source of starch (LPSt; +64 g/kg DM) or RPMet (LPM; +0.3 g/100 g MP). At the end of the 14-wk feeding period, 20 cows (5 per treatment) continued to be fed the same diets for a further 6 d, and total urine output and fecal samples were collected. We observed that dietary treatment did not affect DM intake, with a mean of 21.5 kg/d; however, we also observed an interaction between diet and week with intake being highest in cows fed LPSt in wk 4 and CON in wk 9 and 14. Mean milk yield, 4% fat-corrected milk, and energy-corrected milk were not altered by treatment. Similarly, we found no effect of dietary treatment on milk fat, protein, or lactose content. In contrast, milk and plasma urea concentrations were highest in cows fed CON. The concentration of blood plasma β-hydroxybutyrate was highest in cows receiving LPM and lowest in LPSt. Apparent NUE was 28.6% in cows fed CON and was higher in cows fed any of the low-protein diets (LP, LPSt, or LPM), with a mean value of 34.2%. The sum of milk fatty acids with a chain length below C16:0 was also highest in cows fed CON. We observed that dietary treatment did not affect the apparent whole-tract nutrient digestibility of organic matter, N, neutral detergent fiber, and acid detergent fiber, with mean values of 0.785, 0.659, 0.660, and 0.651 kg/kg respectively, but urinary N excretion was approximately 60 g/d lower in cows fed the low-CP diets compared with CON. We conclude that reducing the CP content of red clover and grass silage-based diets from 175 to 150 g/kg DM while maintaining MP supply did not affect performance, but reduced the urinary N excretion and improved NUE, and that supplementing additional starch or RPMet had little further effect.

Novel quality feed from a wasted resource: measuring the nutritional value of low-glycoalkaloids potato haulm in sheep

This study evaluated the potential of low-glycoalkaloid potato haulm (LGPH) as a high-quality feed for ruminants. The low-glycoalkaloid potato plants were grown in a net house following standard agricultural practices. Dehaulming was performed a fortnight before the harvest of the tubers, as commercially practiced. Four healthy female lambs (aged 4 months) were employed in a 4×4 Latin square feeding experiment design. The lambs were fed with either a maintenance diet consisting of 73% roughage (control), or treatment diets with supplementation of 10% (P10), 15% (P15), and 20% (P20) LGPH on a dry matter (DM) basis for a period of 21 days, including 14 days of adaption and 7 days of sampling. Refusals and feces were collected for 6 days and pooled followed by a 24 h urine collection on the 7th day. On the 6th day, an hour after morning feeding, blood, and rumen liquid samples were collected. All the samples were analysed, and the data generated were analysed using ANOVA with diet and period as fixed effects and sheep as a random effect. Orthogonal contrasts were used to detect linear and quadratic effects of LGPH in the diet. Linear or polynomial equations were produced to extract the nutrient digestibility and metabolizable energy (ME) of LGPH. No toxicological clinical signs were observed, and the haematology profiles were normal throughout the trial. LGPH did not affect the daily intake of nutrients and energy. However, the apparent digestibility of DM, organic matter (OM), crude protein (CP), and gross energy increased (P= 0.01) linearly (P&amp;lt; 0.01) with supplementation of LGPH in the diet. The inclusion of LGPH had a quadratic effect on neutral detergent fiber (NDF) digestibility (P= 0.027), reaching a peak of 54.8 % at 12.3 % LGPH supplement on a DM basis. The N retention in lambs fed with the P20 diet was nearly twice that of the control diet (P= 0.032; 19.1 vs. 10.2 g/d). The computed digestibility values of DM, OM, CP, and NDF for LGPH were 76.1, 79.7, 84.2, and 72.1 %, respectively, and ME was 2.62 Mcal/kg DM. Conclusively, by significantly reducing the glycoalkaloids’ content in potato haulm, we can safely repurpose the currently wasted foliage as a new source of high-quality roughage feed for ruminants, equivalent to alfalfa hay, without imposing any additional environmental burden. However, further research is necessary to assess impact of LGPH on growth performance, and milk productivity before practical application on commercial farms.

Synbiotics improve growth performance and nutrient digestibility, inhibit PEDV infection, and prevent intestinal barrier dysfunction by mediating innate antivirus immune response in weaned piglets.

This experiment was conducted to explore the effects of dietary synbiotics (SYB) supplementation on growth performance, immune function, and intestinal barrier function in piglets challenged with porcine epidemic diarrhea virus (PEDV). Forty crossbred (Duroc × Landrace × Yorkshire) weaned piglets (26 ± 1d old) with a mean body weight (BW) of 6.62 ± 0.36kg were randomly allotted to five groups: control (CON) I and CONII group, both fed basal diet; 0.1% SYB group, 0.2% SYB group, and 0.2% yeast culture (YC) group, fed basal diet supplemented with 0.1%, 0.2% SYB, and 0.2% YC, respectively. On day 22, all piglets were orally administrated with 40mL PEDV (5.6 × 103 TCID50/mL) except piglets in CONI group, which were administrated with the same volume of sterile saline. The trial lasted for 26d. Before PEDV challenge, dietary 0.1% SYB supplementation increased final BW, average daily gain (ADG), and decreased the ratio of feed to gain during 0 to 21d (P < 0.05), as well as improved the apparent nutrient digestibility of dry matter (DM), organic matter (OM), crude protein, ether extract (EE), and gross energy (GE). At the same time, 0.2% YC also improved the apparent nutrient digestibility of DM, OM, EE, and GE (P < 0.05). PEDV challenge increased diarrhea rate and diarrhea indexes while decreased ADG (P < 0.05) from days 22 to 26, and induced systemic and intestinal mucosa innate immune and proinflammatory responses, destroyed intestinal barrier integrity. The decrease in average daily feed intake and ADG induced by PEDV challenge was suppressed by dietary SYB and YC supplementation, and 0.1% SYB had the best-alleviating effect. Dietary 0.1% SYB supplementation also increased serum interleukin (IL)-10, immunoglobulin M, complement component 4, and jejunal mucosal IL-4 levels, while decreased serum diamine oxidase activity compared with CONII group (P < 0.05). Furthermore, 0.1% SYB improved mRNA expressions of claudin-1, zonula occludens protein-1, mucin 2, interferon-γ, interferon regulatory factor-3, signal transducers and activators of transcription (P < 0.05), and protein expression of occludin, and downregulated mRNA expressions of toll-like receptor 3 and tumor necrosis factor-α (P < 0.05) in jejunal mucosa. Supplementing 0.2% SYB or 0.2% YC also had a positive effect on piglets, but the effect was not as good as 0.1% SYB. These results indicated that dietary 0.1% SYB supplementation improved growth performance under normal conditions, and alleviated the inflammatory response and the damage of intestinal barrier via improving innate immune function and decreasing PEDV genomic copies, showed optimal protective effects against PEDV infection.

Dose-dependent effect of spent coffee grounds on intake, apparent digestibility, fermentation pattern, methane emissions, microbial protein supply, and antioxidant status in Latxa sheep.

Spent coffee grounds (SCG), a byproduct rich in polyphenols, can form part of enteric CH4 mitigation strategies while promoting the circular economy. This study aimed to evaluate the effect of 3 levels of SCG inclusion in the concentrate on enteric CH4 production, feed intake, apparent digestibility, ruminal fermentation pattern, microbial protein supply, and gene expression of immune and antioxidant markers in peripheral blood of dry dairy ewes. In a replicated 4 × 4 Latin square design, 8 nonproductive Latxa ewes were assigned to a concentrate that differed in the level of SCG: Control (0g/kg DM), SCG100 (100g/kg DM), SCG150 (150g/kg DM) and SCG200 (200g/kg DM). In each period, 14 d of adaptation were allowed, followed by 7 d in individual metabolic cages, and 3 d in respiratory chambers. To avoid a carry-over effect a minimum of 7 d were allotted between periods in which ewes consumed control concentrate and grass hay. Total organic matter intake (OMI) and CH4 emissions (g/d) presented a quadratic response (P = 0.008 and P < 0.001, respectively) to increasing levels of SCG in the feed. However, when CH4 emissions were corrected for OMI, a linear decrease was observed with increasing levels of SCG in the concentrate (P = 0.009). This reduction in CH4 emissions (g/kg OMI) could be explained by the linear decrease (P = 0.034) observed in the apparent digestibility of organic matter (OM), particularly in crude protein (CP) and starch (P = 0.002 and P = 0.003, respectively), with increasing levels of SCG in the concentrate. No significant response was found on CH4 emissions corrected for digestible OM and on ruminal fermentation pattern. Regarding microbial protein supply, a linear increase in microbial protein supply efficiency (P = 0.008) was observed with increasing levels of SCG in the concentrate. Moreover, SCG inclusion linearly reduced interleukin 10 (P = 0.031), nuclear factor E2-related factor 2 (P = 0.007), nuclear factor kappa β (P = 0.014), superoxide dismutase 1 (P = 0.015) gene expression and tended to linearly reduce those of tumor necrosis factor-α (P = 0.074) and glutathione peroxidase 1 (P = 0.082). In conclusion, inclusion of SCG up to 200g/kg in the concentrate did not modify ruminal fermentation pattern, but linearly reduced CH4 emissions per kg of OMI, due to a linear decrease in the apparent digestibility of CP and starch. Moreover, linearly increased the efficiency of microbial supply and improved sheep's blood antioxidant-immune status.