Two experiments were conducted to examine the effects of conditioning temperature (CT) and the interactive influence of feed acidification (FA) and CT on the performance, coefficient of apparent ileal digestibility (CAID) of nitrogen (N), starch, fat calcium (Ca) and phosphorus (P), and AME in broilers. In both experiments, each treatment was randomly allocated to 6 cages (8 birds per cage) and fed from 1 to 21 D posthatch. In experiment 1, the effect of CT was evaluated using a wheat-based diet at 3 CT: unconditioned, conditioned at 60°C or 90°C. All the diets by-passed the pellet press and collected in mash form. Birds fed the diet conditioned at 90°C consumed more (P < 0.05) feed and tended (P = 0.087) to have higher feed per gain (F:G) than those fed the unconditioned diet but similar to those fed the diet conditioned at 60°C. A tendency was noted for CT to affect the CAID of N (P = 0.071) and starch (P = 0.093), with reduced digestibility values in the diet conditioned at 90°C. Conditioning at 90°C resulted in lower (P < 0.05) AME. In experiment 2, three inclusions of an acidifier (0.0, 7.0, and 10 g/kg) and 2 CT of 60°C and 90°C were evaluated in a 3 × 2 factorial arrangement of treatments using pelleted diets. Neither the main effects nor the interaction between acidifier addition and CT was significant for weight gain, feed intake, and F:G. The FA increased (P < 0.05) the CAID of N, fat, and P at both inclusion levels and of starch at 10 g/kg. Conditioning at 90°C reduced (P < 0.05) the CAID of starch, fat, and Ca, regardless of FA level. Overall, the present data showed that the application of high CT for broiler feed manufacture can impair nutrient utilization and, consequently the feed efficiency in broilers. Feed acidification imparts some benefits to nutrient digestibility in broilers fed pelleted wheat-based diets.