Apparent Change Research Articles

Seasonal movements of Black Coucals Centropus grillii in Nigeria

The scale of movement associated with the migration of many intra-African bird species is still poorly known even with the increasing availability of tracking devices. In this study, we tracked the movements of Black Coucals Centropus grillii breeding in Nigeria from late July using satellite telemetry. Individuals remained on the breeding site for several months; two individuals transmitted after October and these two moved shorter distances (< 100 km) away from the breeding site in early December. One of these was tracked for a full year, moving to a site 175 km south of the breeding site in early January and returning to the breeding site in late May. The bird migrated faster during the return journey (58 km day−1) as compared to the post-breeding journey (5.9 km day−1). The overall home range (90% kernel density) during breeding was 20.4 ± 3.3 km2 (mean ± s.d.) and the core (50%) 5.0 ± 1.6 km2 with no apparent clear change outside of the breeding season. Vegetation conditions at the distant site were apparently poorer although in an average year the move would have led to improved conditions. Short-distance seasonal migration of Black Coucals might be widespread in drier seasonal habitats.

Reversible Sensing Technologies Using Upcycled TPEE: Crafting pH and Light Responsive Materials towards Sustainable Monitoring.

Multiresponsive materials with reversible and durable characteristics are indispensable because of their promising applications in environmental change detections. To fabricate multiresponsive materials in mass production, however, complex reactions and impractical situations are often involved. Herein, a dual responsive (light and pH) spiropyran-based smart sensor fabricated by a simple layer-by-layer (LbL) assembly process from upcycled thermoplastic polyester elastomer (TPEE) materials derived from recycled polyethylene terephthalate (r-PET) is proposed. Positively charged chitosan solutions and negatively charged merocyanine-COOH (MC-COOH) solutions are employed in the LbL assembly technique, forming the chitosan-spiropyran deposited TPEE (TPEE-CH-SP) film. Upon UV irradiation, the spiropyran-COOH (SP-COOH)molecules on the TPEE-CH-SP film undergo the ring-opening isomerization, along with an apparent color change from colorless to purple, to transform into the MC-COOH molecules. By further exposing the TPEE-CH-MC film to hydrogen chloride (HCl) and nitric acid (HNO3) vapors, the MC-COOH molecules can be transformed into protonated merocyanine-COOH (MCH-COOH) with the simultaneous color change from purple to yellow.

Interface structures of Al0.85Sc0.15N-on-Si thin films grown by reactive magnetron sputtering upon post-growth cyclic rapid thermal annealing

Al0.85Sc0.15N thin films, about 920 nm thick, have been deposited on the Si (001) substrate by reactive magnetron sputtering at 600 °C. X-ray diffraction and pole-figure measurements revealed [0002]-oriented texture structures of the nitride films without any phase separations before and after cyclic annealing at 600–900 °C for up to 48 min. Cross-sectional studies by transmission electron microscopy and energy dispersive x-ray analysis revealed an intermediate Al0.85Sc0.15N layer of ∼24.6 nm thick with smaller grains and tilted [0002]-orientations compared to its overlayer, i.e., a nucleation layer (NL), on the Si substrate. After annealing, apparent morphological changes have been observed at the near-interface regions, including the NL, the NL/Si interface, and the Si substrate, rather than in the Al0.85Sc0.15N overlay. Undesired oxygen has been observed in the nitride film and its composition increased during post-growth thermal annealing without forming oxides. These observations shed new light on crystal growth and post-growth thermal annealing of AlScN toward their high-performance piezoelectric applications.

To Explore the Mechanism of Periplaneta americana Extracts and Rebeprazole Intervention in Gastric Mucosal Injury Based on ERS Pathway

The mechanism of Periplaneta americana extracts and rabeprazole in treating gastric mucosal injury were explored through ER stress pathway. Acute gastric mucosal injury model rats were made by intragastric administration of anhydrous ethanol. The rats were then randomly assigned to different groups: model group, Periplaneta americana extracts group, rabeprazole group and combined drug group of rabeprazole and Periplaneta americana extracts, with 6 rats in each group. A normal control group, comprising of six rats, was fed a standard diet. Drug groups were treated with intragastric administration for 3 days. The apparent morphological changes of gastric mucosal injury repair in each group of rats were observed. The length and width of the damaged erosion bands were measured and recorded by vernier caliper, and the index of gastric mucosal damage of rats was calculated using the Guth 57 standard method. Pathological repair of gastric mucosal damage was visualized using hematoxylin-eosin staining (HE). The protein expression of gastric mucosa glucose regulated protein (GRP 78), transcriptional activator 6 (ATF 6), C/EBP (CHOP) and interleukin-6 (IL-6) by protein immunoblot (Western Blot, WB). The content of prostaglandin 2 (PGE 2) in the gastric mucosa and serum was observed by an enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC). The results were compared between the other groups and the model group. Intervention treatment in each experimental group demonstrated effective improvement of gastric mucosal injury, reduction in the index of gastric mucosal injury, downregulation of the protein expression of GRP 78, ATF 6, CHOP, and IL-6 in the gastric mucosa. Increasing the content of PGE2 in gastric mucosa and serum, and promoting the repair of inflammation. Notably, the combined drug group exhibited the most significant intervention effect, with a statistically significant difference (P &gt; 0.05). Periplaneta americana extracts, Rabeprazole alone and in combination have different degrees of protection and repair effect on gastric mucosal injury. The regulation of endoplasmic reticulum stress (ERS) may affect the mechanism of action, reducing the protein expression of inflammatory factors, increasing the expression level of PGE2, and promoting the recovery of normal physiological metabolic environment of gastric mucosa.

Aquaporin-5 Protein Is Selectively Reduced in Rat Parotid Glands under Conditions of Fasting or a Liquid Diet.

Aquaporin-5 (AQP5) water channel, transmembrane protein 16A (TMEM16A) Ca2+-activated Cl- channel, and Na+-K+-2Cl- cotransporter (NKCC1) are membrane proteins on salivary gland acinar cells that function in watery saliva secretion. We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid diet for 48 hr or 1 week to reduce mastication. The parotid glands were then resected to analyze the protein and mRNA levels by immunofluorescence, immunoblotting, and reverse-transcription quantitative PCR (RT-qPCR). AQP5 protein was significantly decreased in both liquid diet groups and the fasting group but its mRNA levels showed no apparent changes compared with the control group. The protein and mRNA levels of TMEM16A and NKCC1 showed no significant changes between any of the groups other than an increase in NKCC1 mRNA in the 1-week liquid diet group. These results suggest that reduced mastication may increase the AQP5 protein degradation, but not that of other membrane proteins necessary for saliva secretion.

High-intensity interval training enhances mRNA expression of IGF1Ea in rat Achilles tendon.

High-intensity interval training (HIIT) reportedly enhances the functional properties of the musculoskeletal system. However, the effects of HIIT on tendons remain unclear. Sixteen male rats were randomly assigned to the control (Con) or HIIT group (n = 8 in each group). Rats in the HIIT group executed the HIIT program consisting of 2.5min treadmill running and 4.5min rests between the bouts, 5days per week for 9weeks. Running speed, number of sets, and inclination were incrementally increased during the training period. Histological analysis revealed no apparent morphological changes in the extracellular matrix structure or nuclei of tenocytes between the groups. Real-time reverse transcription polymerase chain reaction analysis revealed thatIgf1EamRNA expression was enhanced in the HIIT group. Furthermore,Igfbp5mRNA expression tended to be higher in the HIIT group. The 9-week HIIT program enhanced tenogenic Igf1Ea mRNA expression.

A 3D Spherical ETAS Model: A Case Study in the Alaska-Aleutian Region

Abstract This study proposes a hypocentral version of the spherical epidemic-type aftershock sequence (ETAS) model by incorporating hypocentral depth information into the spherical ETAS model and applies it to earthquake data from the Alaska-Aleutian area. We validate the model using the stochastic reconstruction techniques and illustrate the rationale behind selecting the spatial probability density function. Subsequently, we estimate the 3D spatial variations of the background and offspring seismicity in the study region, revealing that the majority of clusters are located in shallow regions. We also identify an apparent temporal change in the background seismicity caused by artificial effects. Compared with the 3D-ETAS and spherical version of the epidemic-type aftershock sequence (SETAS) models, the new 3D-SETAS model provides a better description of 3D seismicity in high-latitude regions globally.

Assessment of the structural change of DNA by binding with a small molecule based on capillary sieving electrophoresis.

In this study, capillary sieving electrophoresis (CSE) using polymer solutions was used to evaluate the structural changes in nucleic acids upon complexation with small molecules. As the model target and nucleic acids, L-tyrosinamide (Tyr-Am) and its aptamer, which is a type of DNA specifically binding to Tyr-Am, were selected. CSE was conducted using a capillary filled with background solution (BGS) containing hydroxypropyl cellulose (HPC) as a sieving matrix. When Tyr-Am or tyrosine was added to the BGS in CSE, the ratio of mobility differences of the Tyr-Am-aptamer complex increased compared to that of the free aptamer without the addition of Tyr-Am. In contrast, when other amino acids or their analogs were added, results showed no apparent change or decreases in electrophoretic mobility. These results indicate that the proposed method can be applied to assess structural changes in nucleic acids that target small molecules.

Incidence of Fetal Arrhythmia Before and During the COVID-19 Pandemic: A Single-Center Experience.

Fetal arrhythmias are rare and carry significant morbidity and mortality without appropriate treatment. Initial reports exist of fetal arrhythmia in the setting of maternal COVID-19 infection. Our study sought to evaluate incidence of fetal arrhythmia before and during the COVID-19 pandemic at our institution. This retrospective cohort study from a tertiary care fetal cardiac center utilized the institutional REDCap database to search fetal arrhythmia diagnostic codes. Medical records of mother-fetus dyads were reviewed and data were collected on diagnoses, gestational age, treatment regimen, and postnatal outcomes. Patients were divided into pre-COVID and peri-COVID segments. 8368 total pregnancies were evaluated during the 7.3years of study period. Forty-five patients (0.5%) had a significant fetal arrhythmia and were included in this study: 19 (42%) in the pre-COVID-19 group and 26 (58%) in the peri-COVID-19 group. No patients had associated congenital heart disease. There was a notable increase in the incidence of fetal supraventricular tachycardia (SVT) (1.82 per 1000 vs 2.65 per 1000 pregnancies) and complete heart block (1.04 per 1000 vs 1.77 per 1000 pregnancies) but no apparent change in other tachyarrhythmias during the COVID era. The proportion of antibody-mediated complete heart block increased from 50 to 87.5%. There was also an increase in the percentage of SVT patients requiring postnatal treatment during COVID-19 (53.8% vs 62.5%). Our experience shows an increased incidence of some fetal arrhythmia diagnoses during the COVID-19 pandemic. Additional multi-center studies will be necessary to fully evaluate the increased burden of fetal arrhythmias during the COVID-19 era as well as to elucidate etiology.

Water, starch, and nuclear behavior in ray parenchyma during heartwood formation of Catalpa bungei ‘Jinsi’

Catalpa bungei ‘Jinsi’, a cultivar of C. bungei C. A. Mey., is valued for its heartwood with good overall mechanical properties, naturally durable and golden-yellow color. Little is known about heartwood formation in C. bungei ‘Jinsi’ trees. The behavior of starch, water, and nuclei was studied in the xylem tissue of C. bungei ‘Jinsi’ concerning aging in ray parenchyma cells. Blocks containing heartwood, golden zone, transition zone, and sapwood were collected from the stems of six C. bungei ‘Jinsi’ trees. The moisture content of the blocks was measured by oven drying. Changes in starch and nuclei in ray parenchyma were investigated in radial profiles from sapwood to heartwood blocks using microscopy and various staining techniques. The nuclear size and starch content gradually decreased to heartwood. While the horizontal distribution of moisture content of C. bungei ‘Jinsi’ was very varied, with the heartwood and golden zone being lower than sapwood but slightly higher than the transition zone. Starch grains were rare, but nuclei were still present in some ray parenchyma cells in the heartwood and golden zone. The nuclei showed irregular shape and elongation before disintegration. These results suggest that the most apparent change occurs in the transition zone, the critical location involved in forming C. bungei ‘Jinsi’ heartwood. Water and starch appear to be actively engaged in heartwood formation. The loss of function of ray parenchyma cells results from heartwood formation.

Manganese (II) Halides for X‐Ray Imaging and Moisture Detection

AbstractOrganic–inorganic hybrid metal halides are widely used in X‐ray detection and imaging due to their high X‐ray absorption efficiency, ease of synthesis, and efficient luminescence. However, the issue of lead toxicity remains a concern. In this study, non‐toxic, 0D 2,2′‐(Ethylenedioxy)bis(ethylamine) manganese (II) bromide (EDBEMnBr4) is synthesized using the simple mechanical grinding method and the solution method that exhibits intense green emission at ≈525 nm. Moreover, the change of structure under the influence of temperature and water significantly affects its fluorescence color under UV light. Scintillator materials with and without moisture exposure showed spatial resolutions of 7 and 4 lp mm−1, and low detection limits of 4.62 and 5.25 µGyair s−1, respectively, meeting medical industry standards (&lt;5.5 µGyair s−1). Furthermore, based on the apparent change in luminescence color triggered by moisture for EDBEMnBr4, a preliminary attempt is made to apply it to humidity monitoring showing that the boundary of color change has a good linear dependence on humidity and time. This work provides new opportunities for Mn‐based metal halides in both X‐ray detection as well as moisture detection.

Dominant role of laser-generated nano-structures on enhancement of interfacial bonding strength by laser surface modification

Laser surface modification has been widely applied to the pre-treatment of material surfaces for interface bonding, however, laser-treated surfaces face an inevitable risk of environmental exposure during engineering application, and it remains unclear how laser-induced modifications change under environmental exposure. This study investigates the robustness of laser-treated aluminum surface under typical atmospheric and aqueous environments at different temperature from −40 °C to 80 °C. Although the carbon elemental content and water contact angle greatly fluctuate with exposure environments from 23 at.% to 33 at.%, and from 0° to 148°, respectively, laser-treated surfaces exhibit stable improvement of ∼25 % in bonding strength compared to untreated condition even after 30 days exposure. Notably, hygrothermal exposure of 80 °C and 95 %RH significantly declines bonding strength by ∼45 %, and this strength degradation is accompanied by 1.8 % increase of carbon elemental content and water contact angle of ∼150°. Results reveal that laser-generated nano-structures play crucial role in strength improvement, instead of carbonaceous contaminants and surface polarity, and nanostructure transformation causes an apparent strength degradation and change of fracture mode from cohesive to interface. This work uncovers the dominant role of laser-generated nano-structures in enhancing interfacial bonding strength, and provides supports for engineering application of laser surface modification.

Differentiating Leaf Structures and Physiological Responses to Freezing Stress of Mangrove Kandelia obovata from Different Provenances

Kandelia obovata (Rhizophoraceae) is the most cold-tolerant mangrove species and has been widely used in coastal wetland ecological restoration due to its specific viviparous phenomenon, beautiful shape, and unique floral pattern. Due to long-term adaptation to their local environment, the phenotypic characteristics and stress resistance of widely distributed plants of the same species often differentiate across different locations. The capacity for cold resistance is closely linked to the physiological and structural characteristics of plants. Herein, we explored the temporal variations in the leaf structure and physiological status of K. obovata under −5.5 °C from different areas such as Jiulongjiang Estuary (JLJ, 24°25′ N), Fujian Province, and Longgang City (LG, 27°34′ N) and Jiaojiang District (JJ, 28°67′ N), Zhejiang Province. The morphological variations implied that the cold resistance of K. obovata obviously strengthened after the northward migration and acclimatization, in the following order: LG &gt; JJ &gt; JLJ. More specifically, after exposure to a sustained low temperature, the relative conductivity (REC), an index widely used to evaluate the degree of plant damage, remarkably increased from 33.62 ± 2.39 to 63.73 ± 3.81, 31.20 ± 1.63 to 49.48 ± 1.12, and 23.75 ± 0.13 to 54.24 ± 1.45 for JLJ, LG, and JJ, respectively (p &lt; 0.05). Additionally, the palisade-to-spongy tissue ratio (P/I) of JLJ and JJ decreased from 0.78 ± 0.05 and 0.75 ± 0.03 to 0.5 ± 0.04 and 0.64 ± 0.02 (p &lt; 0.05), whereas no significant changes were found in LG (p &gt; 0.05). The SOD activity of LG significantly kept increasing, with values increased from 352.49 ± 10.38 to 477.65 ± 1.78 U·g−1, whereas no apparent changes in JLJ and JJ were observed with the sustained low temperature. The results of this study improved our understanding of the response of K. obovata to freezing stress, which could provide a sound theoretical foundation for cultivating cold-resistant varieties, as well as expanding mangrove plantations in higher latitudes.

Metabolomic responses to high-intensity interval exercise in equine skeletal muscle: effects of rest interval duration.

High-intensity interval training has attracted considerable attention as a time-efficient strategy for inducing physiological adaptations, but the underlying mechanisms have yet to be elucidated. By using metabolomics techniques, we investigated changes in the metabolic network responses in Thoroughbred horses to high-intensity interval exercise performed with two distinct (15 min or 2 min) rest intervals. The peak plasma lactate level was higher during high-intensity exercise with a 2 min rest duration than that with a 15 min rest duration (24.5±6.8 versus 13.3±2.7 mmol l-1). The arterial oxygen saturation was lower at the end of all exercise sessions with a 2 min rest duration than that with a 15 min rest duration. Metabolomic analysis of skeletal muscle revealed marked changes in metabolite concentrations in the first and third bouts of the 15 min rest interval conditions. In contrast, there were no metabolite concentrations or pathways that significantly changed during the third bout of exercise performed with a 2 min rest interval. Our findings suggest that the activity of each energy production system is not necessarily reflected by apparent changes in metabolite concentrations, potentially due in part to a better match between metabolite flux into and out of the pathway and cycle, as well as between metabolite production and disposal. This study provides evidence that changes in metabolite concentrations vary greatly depending on the number of repetitions and the length of rest periods between exercises, even if the exercises themselves are identical.

Seasonal Changes of Common Viral Infections in Children After Their Return to School During COVID-19 Pandemic

Abstract Introduction During the COVID-19 pandemic, return of children and adolescents to schools and colleges, with infections preventive restrictions, occurred in September 2020. In spring 2021, these restrictions were lifted. In this context, health systems noticed an apparent change in the seasonality and epidemiology of common viral infections in both adult and pediatric populations. Methods We retrospectively analyzed the results of respiratory virus polymerase chain reaction (PCR) carried out on pediatric nasopharynx samples, tested with multiplex PCR assay to detect the presence of common respiratory viruses. Results We reviewed a total of 1338 PCR samples from our microbiology laboratory. After the return to school, the most frequently identified virus was rhinovirus-enterovirus (27.8%), followed by respiratory syncytial virus (21%). An increase of positive respiratory syncytial virus PCRs was detected out of its common epidemic period from spring to summer of 2021. A peak in influenza virus infections was observed between March and May 2022. The lifting of restrictions due to COVID-19 had a significant impact on the type of virus detected. Conclusions We conclude that with the end of the COVID-19 pandemic, the return to school of children and adolescents, and lifting of preventative restrictions, the circulation of common viruses within this age group increased gradually, showing an unusual delay and peaks outside its epidemic periods.

Anodic leaching plus chronopotentiometric aging of Ni2Fe ingot for sustainable oxygen evolution and urea oxidation reactions

A Ni–Fe binary alloy ingot, prepared by conventional vacuum smelting, was adopted as substrate in this work to fabricate the anode for water splitting. To improve the surface roughness of anode surface, the electrode was firstly subjected to anodic leaching in a simple KSCN solution. The as-leached electrode was subsequently activated through linear sweep voltammetry in 1 M KOH solution with or without 0.33 M urea addition to finish the final step for electrode preparation. The as-prepared anode delivers the 10 mA cm−2 current density at an overpotential of 284 mV for oxygen evolution reaction (OER) and at 1.361 V (RHE) for urea oxidation reaction (UOR), respectively. No apparent change of OER and UOR performance was observed after chronopotentiometric aging. The more complex UOR behavior was analyzed to elucidate the synergy among all constituents functioning through direct or indirect mechanisms for the half-reaction on this binder-free, self-supporting and substrate-rejuvenating anode.

Solvent-Free Method for Masking the Bitter Taste of Azithromycin Dihydrate Using Supercritical Fluid Technology

ABSTRACT Introduction and purpose The unpleasant extremely bitter taste of the orally administered broad-spectrum antibiotic azithromycin decreases patient compliance, especially in pediatrics. This issue can be overcome by decreasing drug interaction with the tasting buds using insoluble polymers at salivary pH (6.8 -7.4), like the cationic polymer Eudragit EPO. Supercritical fluid technology is a green synthesis method for preparing pharmaceutical preparations that replace organic solvents with safe supercritical CO2. This study aimed to mask the bitter taste of azithromycin using the supercritical fluid method and a pH-sensitive Eudragit EPO polymer. Methods: A foaming process was investigated for preparing a formulation (TEST), which comprises treating the polymer with supercritical carbon dioxide (CO2) fluid to prepare a taste-masked dosage form without employing organic solvents or flavors. Results: The use of the supercritical technique at 40 °C and 10 MPa for two hours allowed the manufacturing of solvent-free polymeric foam with azithromycin dispersions; the average calculated percentage of apparent volume change was 62.5 ± 5.9% with an average pore diameter of 34.879 Å. The formulated sample showed low drug release in simulated salivary fluid while keeping its crystalline nature. Moreover, clinical studies on healthy subjects showed that the formula successfully masked azithromycin's bitter taste. Conclusions: Overall, it has been shown herein that the supercritical fluid technology foaming method is promising in masking the bitter taste of bitter ingredients.

A direct-AMS assessment of Re-Os in a Cigar Lake uranium ore specimen

The decay constant of 187Re is known to change when its inner shell electrons are ionized; the possibility of a small change occurring in a terrestrial radioactive environment has not been assessed. A recently completed multi-isotope survey of uranium ore concentrates (UOC) has prompted the question. As an extension of that work, using direct accelerator mass spectrometry (direct-AMS), this paper reports a closer investigation of such possibility in an unaltered natural rock specimen of the Cigar Lake uranium ore, containing wt.30–60 % uranium oxide. Stimulated by this research goal, the direct-AMS quantification methods were first investigated, and later tested in connection with the Re-Os assessment. Complete sets of measurement of the anion beams of 185ReOn−, (187Re+187Os)On− and 189OsOn− (n = 0–5) were carried out to find suitable sputter target matrices that would yield self-consistent solutions of (187Os/189Os)Today and (187Re/185Re)Today. These two quantities are best determined by these multi-beam measurements to be 4400 ± 500 (2σ) and 1.635 ± 0.060 (2σ), respectively. The sample is then reasoned to contain [Re/Os]Initial ∼42,000. These results do not show apparent 187Re decay constant changes, but cannot rule out the possibility, either. As a quick follow-up inspection, a simplified approach was used to surveying 187Re/185Re in a series of uranium-concentrated materials, in which this ratio was all measured somewhat smaller than the natural known value. This work was also intended to be a pilot feasibility study of using the direct-AMS method as a form of sensitive secondary ionization mass spectrometry (SIMS) for rapid Re-Os assessment in mg-sized raw minerals and environmental samples.

Between Redemption and Retribution: Justifying Commutations for Life-without-parole Sentences in California

For persons serving life-without-parole (LWOP) sentences in California, a commutation usually offers them the sole glimpse of hope for release from prison. While governors were reluctant to consider any sentence reductions from 1975 to 2016, commutations—including those for LWOP—have become a more frequent occurrence since. Yet, little is still known about how governors have justified reducing a sentence that initially offered no prospect of release from prison. Given the apparent change in practice, themes emerging from the content of 177 gubernatorial commutation summaries granted to persons serving LWOP sentences between 2017 and 2021 are analyzed in this paper. Through open coding, rehabilitative indicators (programming and institutional conduct) emerged as necessary preconditions for a commutation, while retributive factors (a reassessment of the nature of the crime and the prerogative to adjust excessive prison sentences or to correct sentencing errors) complemented sentence reductions. The findings expose the ethical issues that arise from an LWOP sentence and the subsequent commutation. More specifically, they shed light on the commitment offenses underlying commuted LWOP sentences and raise questions regarding the penal objectives that are supposed to be accomplished with permanent imprisonment.

The Learning Curve for Endoscopic Endonasal Transsphenoidal Pituitary Surgery: Evaluating Endocrine Outcomes

ObjectiveIn South Australia endoscopic endonasal approach (EEA) pituitary surgery has been practiced since 2006, largely by two neurosurgeons with a small fellowship-trained otolaryngology team. The aim of this cohort study was to determine if a “learning curve” can be established over this time period, as represented by structural and endocrine patient outcomes. MethodRetrospective cohort study of patients undergoing EEA surgery between 2006 and 2021 in Adelaide, South Australia at three tertiary teaching hospitals.. Cases were divided by each surgeon and split into groups of sequential 40 cases. Endocrine assessment pre- and post-operatively involved static pituitary and end-organ hormones, with dynamic tests as required, assessed by an pituitary endocrinologist. Each hormonal axis (gonadal, cortisol, thyroid, prolactin and growth hormone) was documented preoperatively and at an early and long term follow up at 1–2 and 12 months, respectively. ResultsThe study included a cohort of 443 pituitary adenomas managed with endoscopic endonasal transsphenoidal surgery in a consecutive fashion between two neurosurgeons over 16-years. Gross tumour resection but not visual visual outcomes improved with surgical experience but this outcome may be neurosurgeon dependent. Endocrine outcomes were not consistently improved with experience, but lower rates of hypopituitarism were seen with experience with one neurosurgeon. Average follow up was approximately 5 years, and a minimum follow up of 12 months for all patients. ConclusionsWe present long term endocrine follow up for patients with functional and non-functional adenomas. Improved rates of gross tumour resection were evident with with surgical experience. However, there was no apparent change in post-operative endocrine outcomes.