Apparent Bone Density Research Articles

Patient-specific finite element analysis of four different fixation methods for transversely unstable radial head fractures

Plate fixation is a common treatment option for radial head fractures (RHFs). Due to the benefits of less invasiveness and fewer complications of internal fixation, the application of small-diameter headless compression screws (HCSs) to treat RHFs has become a new trend. This study aimed to compare the mechanical stability of four distinct internal fixation protocols for transversely unstable RHFs via finite element analysis. Using computed tomography data from 10 patients, we developed 40 patient-specific FE models of transversely unstable RHFs fixed by parallel, crossed, and tripod HCSs and mini-T plate (MTP). Under simulated physiological loading of the elbow joint, the construct stiffness, displacement, and von Mises stresses were evaluated and verified by a biomechanical experiment. Under shear loading, the MTP group exhibited lower construct stiffness, larger displacement, and higher Von Mises stress than the HCSs group. The stiffness of tripod HCSs was greater than parallel and crossed screw fixation techniques. There was a strong relationship between apparent bone density and construct stiffness (R = 0.98 to 0.99). In the treatment of transversely unstable RHFs, HCSs have superior biomechanical stability than MTP. The tripod technique was also more stable than parallel and crossed fixation.

INTRAMEDULLARY NAIL OR LOCKING COMPRESSION PLATE FOR FIXING A FRACTURED DISTAL TIBIA: FINITE ELEMENT ANALYSIS ALONG WITH AN ADAPTATION MODEL

Intramedullary (IM) nailing and plating are two major options for the treatment of distal tibial fractures; each method has its own pros and cons. Bone loss due to IM nailing or plating can be a serious concern, which may lead to the premature failure of the implants. Thus, investigating bone remodeling in response to orthopedic implantation is of paramount importance for the purpose of designing more efficient prostheses. The main goal of this study was to predict the effects of nailing and plating on the bone apparent density distribution in tibia, through use of the finite element method (FEM) along with a bone remodeling model. To this end, three-dimensional geometry of tibia, with properties derived from computer tomography (CT) imaging data, IM nail and locking compression plate (LCP) were created. Then, a bone remodeling model and FEM, with physiological-like loading, were combined and implemented using Python programming language. The results showed that both nailing and plating methods cause a reduction in bone apparent density, but the plate affects just the distal part of the tibia, where the implant is present; whereas the IM nail affects almost the whole tibia. Moreover, in agreement with clinical observations, it was found here that the stress shielding effects of the LCP are less pronounced than that of IM nail, in the long term. Stress analysis revealed that the load, to most extent, transferred to IM nail; whereas it transferred to LCP just in the distal part of tibia. On the basis of the results of this work, it might be concluded that plating is a better choice than nailing for distal tibial fracture, from the bone remodeling point of view.

BONE REMODELLING PREDICTION FOR IMPROVED POROUS IMPLANT DESIGN

AbstractObjectivesYoung patients receiving metallic bone implants after surgical resection of bone cancer require implants that last into adulthood, and ideally life-long. Porous implants with similar stiffness to bone can promote bone ingrowth and thus beneficial clinical outcomes. A mechanical remodelling stimulus, strain energy density (SED), is thought to be the primary control variable of the process of bone growth into porous implants. The sequential process of bone growth needs to be taken into account to develop an accurate and validated bone remodelling algorithm, which can be employed to improve porous implant design and achieve better clinical outcomes.MethodsA bone remodelling algorithm was developed, incorporating the concept of bone connectivity (sequential growth of bone from existing bone) to make the algorithm more physiologically relevant. The algorithm includes adaptive elastic modulus based on apparent bone density, using a node-based model to simulate local remodelling variations while alleviating numerical checkerboard problems. Strain energy density (SED) incorporating stress and strain effects in all directions was used as the primary stimulus for bone remodelling. The simulations were developed to run in MATLAB interfacing with the commercial FEA software ABAQUS and Python. The algorithm was applied to predict bone ingrowth into a porous implant for comparison against data from a sheep model.ResultsThe accuracy of the predicted bone remodelling was verified for standard loading cases (bending, torsion) against analytical calculations. Good convergence was achieved. The algorithm predicted good bone remodelling and growth into the investigated porous implant. Using the standard algorithm without connectivity, bone started to remodel at locations unconnected to any bone, which is physiologically implausible. The implementation of bone connectivity ensures the gradual process of bone growth into the implant pores from the sides. The bone connectivity algorithm predicted that the full remodelling required more time (approximately 50% longer), which should be considered when developing post-surgical rehabilitation strategies for patients. Both algorithms with and without bone connectivity implementation converged to same final stiffness (less than 0.01% difference). Almost all nodes reached the same density value, with only a limited number of nodes (less than 1%) in transition areas with a strong density gradient having noticeable differences.ConclusionsAn improved bone remodelling algorithm based on strain energy density that modelled the sequential process of bone growth has been developed and tested. For a porous metallic bone implant the same final bone density distribution as for the original adaptive elasticity theory was predicted, with a slower and more fidelic process of growth from existing surrounding bone into the porous implant.Declaration of Interest(b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

On interaction between fatigue of reconstruction plate and time-dependent bone remodeling

Background and objectiveThe fibula free flap (FFF) has been extensively used to repair large segmental bone defects in the maxillofacial region. The reconstruction plate plays a key role in maintaining stability and load-sharing while the fibula unites with adjacent bone in the course of healing and remodeling. However, not all fibula flaps would fully unite, and fatigue of prosthetic devices has been recognized as one major concern for long-term load-bearing applications. This study aims to develop a numerical approach for predicting the fatigue life of the reconstruction plate by taking into account the effect of ongoing bone remodeling. MethodsThe patient-specific mandible reconstruction with a prosthetic system is studied in this work. The 3D finite element model with heterogeneous material properties obtained from clinical computerized tomography (CT) data is developed for bone, and eXtended Finite Element Method (XFEM) is adopted for the fatigue analysis of the plate. During the remodeling process, the changing apparent density and Young's modulus of bone are simulated in a step-wise fashion on the basis of Wolff's law, which is correlated with the specific clinical follow-up. The maximum biting forces were considered as the driving force on the bone remodeling, which are measured clinically at different time points (4, 16 and 28 months) after reconstruction surgery. ResultsUnder various occlusal loadings, the interaction between fatigue crack growth and bone remodeling is investigated to gain new insights for the future design of prosthetic devices. The simulation results reveal that appropriate remodeling of grafted bone could extend the fatigue life of fixation plates in a positive way. On the other hand, the rising occlusal load associated with healing and remodeling could lead to fatigue fracture of fixation plate and potentially cause severe bone resorption. ConclusionThis study proposes an effective approach for more realistically predicting fatigue life of prosthetic devices subject to a tissue remodeling condition in-silico. It is anticipated to provide a guideline for deriving an optimal design of patient-specific prosthetic devices to better ensure longevity.

Biomechanical effects of bone quality and design features in dental implants in long-term bone stability

Abstract This study analyzed the effects of implant design factors (diameter, length, and connection type) and bone quality on the long-term success of implantation via finite element (FE) analysis, considering the bone remodeling process. A total of 36 cases of the three-dimensional FE model, with an implant complex located in the mandibular bone section of the molar region, were considered. An FE analysis considering dental bone remodeling was implemented in the Abaqus UMAT subroutine. A preload of 32 N∙cm and an average biting force of 50 N were applied during the bone remodeling process as the loading conditions. After the bone remodeling process, a maximum biting force of 200 N was applied in the oblique direction. The stability of the peri-implant bone was analyzed by measuring the apparent bone density over time and the principal strain in the cancellous bone. The stability of the peri-implant bone differs depending on the host bone conditions and implant design features. The initial bone condition greatly influenced the final density and maximum principal strain distribution in the cancellous bone surrounding the implant. The need for a stability evaluation considering the bone remodeling process has increased under the conditions of placing short-length and narrow-diameter implants in poor-quality bones.

Simple anisotropic model of Bone Adaptation - SAMBA

Bone presents the ability to adapt itself to the evolving mechanical environment. A simple anisotropic model for bone adaptation allowing reproducing the evolution of the elastic properties and the reorientation of the anisotropy frame is proposed is this work. The elastic properties are related to the value of the bone apparent density. The evolution law of the density is described via two functions reflecting the activities of the osteoclast and osteoblast cells. The anisotropy of the elastic properties of the bone is assumed evolving continuously between those of trabecular and compact tissues. The existence of a target material frame is assumed to describe its reorientation. The rate of rotation or spin of the material frame is supposed to be proportional to the target orientation angle and to the activity of the osteoclasts and osteoblasts. The mechanical stimulus governing the evolution of the apparent density is defined as the ratio between the current strain energy density and its critical value corresponding to the initiation of bone damage. The simulations showed that this simple model can reproduce some essential phenomena observed during bone adaptation process.

Effect of osteoporosis-related reduction in the mechanical properties of bone on the acetabular fracture during a sideways fall: A parametric finite element approach.

The incidence of acetabular fractures due to low-energy falls is increasing among the geriatric population. Studies have shown that several biomechanical factors such as body configuration, impact velocity, and trochanteric soft-tissue thickness contribute to the severity and type of acetabular fracture. The effect of reduction in apparent density and elastic modulus of bone as well as other bone mechanical properties due to osteoporosis on low-energy acetabular fractures has not been investigated. The current comprehensive finite element study aimed to study the effect of reduction in bone mechanical properties (trabecular, cortical, and trabecular + cortical) on the risk and type of acetabular fracture. Also, the effect of reduction in the mechanical properties of bone on the load-transferring mechanism within the pelvic girdle was examined. We observed that while the reduction in the mechanical properties of trabecular bone considerably affects the severity and area of trabecular bone failure, reduction in mechanical properties of cortical bone moderately influences both cortical and trabecular bone failure. The results also indicated that by reducing bone mechanical properties, the type of acetabular fracture turns from elementary to associated, which requires a more extensive intervention and rehabilitation period. Finally, we observed that the cortical bone plays a substantial role in load transfer, and by increasing reduction in the mechanical properties of cortical bone, a greater share of load is transmitted toward the pubic symphysis. This study increases our understanding of the effect of osteoporosis progression on the incidence of low-energy acetabular fractures. The osteoporosis-related reduction in the mechanical properties of cortical bone appears to affect both the cortical and trabecular bones. Also, during the extreme reduction in the mechanical properties of bone, the acetabular fracture type will be more complicated. Finally, during the final stages of osteoporosis (high reduction in mechanical properties of bone) a smaller share of impact load is transferred by impact-side hemipelvis to the sacrum, therefore, an osteoporotic pelvis might mitigate the risk of sacral fracture.

Bone remodelling in implanted proximal femur using topology optimization and parameterized cellular model

The clinical relevance of bone remodelling predictions calls for accurate finite element (FE) modelling of implant-bone structure and musculoskeletal loading conditions. However, simplifications in muscle loading, material properties, has often been used in FE simulations. Bone adaptation induces changes in bone apparent density and its microstructure. Multiscale simulations, involving optimization methods and biomimetic microstructural models, have proven to be promising for predicting changes in bone morphology. The objective of the study is to develop a novel computational framework to predict bone remodelling around an uncemented femoral implant, using multiscale topology optimization and a parameterized cellular model. The efficacy of the scheme was evaluated by comparing the remodelling predictions with those of isotropic strain energy density (SED) and orthotropy based formulations. The characteristic functional groups and low-density regions of Ward's triangle, predicted by the optimization scheme, were comparable to micro-CT images of the proximal femur. Although the optimization scheme predicted well comparable material distribution in the 2D femur models, the obscured material orientations in some planes of the 3D model indicate the need for a more robust modelling of the boundary conditions. Regression analysis revealed a higher correlation (0.6472) between the topology optimization and SED models than the orthotropic predictions (0.4219). Despite higher bone apposition of 10–20% around the distal tip of the implant, the bone density distributions were well comparable to clinical observations towards the proximal femur. The proposed computational scheme appears to be a viable method for including bone anisotropy in the remodelling formulation.

Predicting bone remodeling using a mechano-biological mathematical model combined with a natural neighbor meshless method

Bone tissue perceives certain mechanical stimuli, adapting its structure to a loading scenario through bone remodeling. Thanks to this ability, bone's functions of support and protection are assured. The mechanical response of bone is computationally reproduced in this work with a new bone remodeling model. Through a mechanobiological approach, the model includes two types of bone cells (i.e. osteoclasts and osteoblasts), describing its functioning and interaction with new sets of parameters. Mechanical stimulation induces a response by bone cells that ultimately is translated into variations of bone's apparent density and other mechanical properties by applying a material phenomenological law. The new algorithm here proposed is for the first time combined with an estabilished meshless method (Natural Neighbor Radial Point Interpolation Method), taking advantage of its features for biomedical applications. As a preliminary study, a two-dimensional bone patch is analyzed. A first study is performed to find the best parameters of NNRPIM for this type of simulation. Then, starting from a uniform bone distribution, a well-defined loading regime is imposed to the bone patch. Results revealed the expected bone adaption, attaining an optimized trabecular structure that reflects the orientation of the applied loads.

Load adaptation through bone remodeling: a mechanobiological model coupled with the finite element method.

This work proposes a novel tissue-scale mechanobiological model of bone remodeling to study bone's adaptation to distinct loading conditions. The devised algorithm describes the mechanosensitivity of bone and its impact on bone cells' functioning through distinct signaling factors. In this study, remodeling is mechanically ruled by variations of the strain energy density (SED) of bone, which is determined by performing a linear elastostatic analysis combined with the finite element method. Depending on the SED levels and on a set of biological signaling factors ([Formula: see text] parameters), osteoclasts and osteoblasts can be mechanically triggered. To reproduce this phenomenon, this work proposes a new set of [Formula: see text] parameters. The combined response of osteoclasts and osteoblasts will then affect bone's apparent density, which is correlated with other mechanical properties of bone, through a phenomenological law. Thus, this novel model proposes a constant interplay between the mechanical and biological components of the process. The spatiotemporal simulation used to validate this new approach is a benchmark example composed by two distinct phases: (1) pre-orientation and (2) load adaptation. On both of them, bone is able to adapt its morphology according to the loading condition, achieving the required trabecular distribution to withstand the applied loads. Moreover, the equilibrium morphology reflects the orientation of the load. These preliminary results support the new approach proposed in this study.

Finite element analysis of bone remodelling with piezoelectric effects using an open-source framework

Bone tissue exhibits piezoelectric properties and thus is capable of transforming mechanical stress into electrical potential. Piezoelectricity has been shown to play a vital role in bone adaptation and remodelling processes. Therefore, to better understand the interplay between mechanical and electrical stimulation during these processes, strain-adaptive bone remodelling models without and with considering the piezoelectric effect were simulated using the Python-based open-source software framework. To discretise numerical attributes, the finite element method (FEM) was used for the spatial variables and an explicit Euler scheme for the temporal derivatives. The predicted bone apparent density distributions were qualitatively and quantitatively evaluated against the radiographic scan of a human proximal femur and the bone apparent density calculated using a bone mineral density (BMD) calibration phantom, respectively. Additionally, the effect of the initial bone density on the resulting predicted density distribution was investigated globally and locally. The simulation results showed that the electrically stimulated bone surface enhanced bone deposition and these are in good agreement with previous findings from the literature. Moreover, mechanical stimuli due to daily physical activities could be supported by therapeutic electrical stimulation to reduce bone loss in case of physical impairment or osteoporosis. The bone remodelling algorithm implemented using an open-source software framework facilitates easy accessibility and reproducibility of finite element analysis made.

Subchondral bone microarchitecture and mineral density in human osteoarthritis and osteoporosis: A regional and compartmental analysis.

Osteoarthritis (OA) and osteoporosis (OP) are historically considered to be inversely correlated but there may be an overlap between the pathophysiology of the two diseases. This study aimed to investigate the subchondral bone microarchitecture and matrix mineralization, and the association between them in OA and OP in relation to the degree of cartilage degeneration. Fifty-six osteochondral plugs were collected from 16 OA femoral heads. They were graded on a regional basis according to the stages of cartilage degeneration, as evaluated by a new macroscopic and a modified microscopic grading system. Twenty-one plugs were collected from seven femoral heads with OP. Plugs were scanned by microcomputed tomography and the microarchitectural and mineral properties were obtained for both subchondral plate and trabecular bone. Microarchitecture and material and apparent densities of subchondral bone in OP were similar to regions with early cartilage degeneration but different from regions with advanced cartilage degradation in OA femoral heads. Subchondral trabecular bone was more mineralized than subchondral plate in both OP and OA, and this compartmental difference varied by severity of cartilage degradation. Furthermore, the relationship among trabecular bone volume fraction, tissue mineral density, and apparent bone density was similar in OP and different stages of OA. Subchondral bone microarchitecture and mineral properties in OP are different from OA in a regionalized manner in relation to stages of cartilage degeneration. Both regional and compartmental differences at structural, material, and cellular levels need to be studied to understand the transition of OA subchondral bone from being osteoporotic to sclerotic.

Regional apparent density correlations within the proximal humerus.

BackgroundBone quality influences humeral implant selection for shoulder arthroplasty. However, little is known about how well bone near the humeral resection represents more distal cancellous bone. This investigation aimed to quantify the correlations between the apparent density of sites near the humeral head resection plane and cancellous sites throughout the metaphysis.MethodsUsing computed tomography data from 98 subjects, apparent bone density was quantified in 65 regions throughout the proximal humerus. Pearson's correlation coefficient was determined comparing the density between samples from the humeral resection and all supporting regions beneath the resection. Mean correlation coefficients were compared for (i) each sample region with all support regions, (ii) pooling all sample regions within a slice, and (iii) considering sample regions correlated with only the support regions in the same anatomic section.ResultsStronger correlations existed for bone sampled beneath the resection (0.33 ± 0.10≤ r ≤ 0.88 ± 0.10), instead of from the resected humeral head (0.22 ± 0.10≤ r ≤ 0.66 ± 0.14). None of sample region correlated strongly with all support regions; however, strong correlations existed when sample and support regions both came from the same anatomic section.DiscussionAssessments of cancellous bone quality in the proximal humerus should be made beneath the humeral resection not in the resected humeral head; and each anatomic quadrant should be assessed independently.

A mathematical biomechanical model for bone remodeling integrated with a radial point interpolating meshless method

Bone remodeling is a highly complex process, in which bone cells interact and regulate bone's apparent density as a response to several external and internal stimuli. In this work, this process is numerically described using a novel 2D biomechanical model. Some of the new features in this model are (i) the mathematical parameters used to determine bone's apparent density and cellular density; (ii) an automatic boundary recognition step to spatially control bone remodeling and (iii) an approach to mimic the mechanical transduction to osteoclasts and osteoblasts. Moreover, this model is combined with a meshless approach – the Radial Point Interpolation Method (RPIM). The use of RPIM is an asset for this application, especially in the construction of the boundary maps. This work studies bone's adaptation to a certain loading regime through bone resorption. The signaling pathways of bone cells are dependent on the level of strain energy density (SED) in bone. So, when SED changes, bone cells' functioning is affected, causing also changes on bone's apparent density. With this model, bone is able to achieve an equilibrium state, optimizing its structure to withstand the applied loads. Results suggest that this model has the potential to provide high quality solutions while being a simpler alternative to more complex bone remodeling models in the literature.

MR imaging pattern of tibial subchondral bone structure: considerations of meniscal coverage and integrity

To compare regional differences in subchondral trabecular structure using high-resolution MRI in meniscus-covered/meniscus-uncovered tibia in cadaveric knees with intact/torn menisci. 3D proton density CUBE MRI of 6 cadaveric knees without significant osteoarthritis (OA) was acquired, 0.25-mm resolution. Menisci were evaluated and classified intact or torn. MR data were transferred to ImageJ program to segment tibial 3D volume of interest (VOI). Data was subdivided into meniscus-covered/meniscus-uncovered regions. Segmented VOI was classified into binary data, trabeculae/bone marrow. The trabecular bone data was used to measure MR biomarkers (apparent subchondral plate-connected bone density (adapted from spine MR), apparent trabecular bone volume fraction, apparent mean trabecular thickness, apparent connectivity density, and structure model index (SMI)). Mean value of parameters was analyzed for the effects of meniscal tear/tibial coverage. Nine torn menisci and 3 intact menisci were present. MR measures of bone varied significantly due to meniscal coverage/tear. Subchondral plate-connected bone density under covered meniscus regions increased from 10.9 to 23.5% with meniscal tear. Values increased in uncovered regions, 19.3% (intact) and 32.4% (torn). This reflects higher density when uncovered (p = 0.048) with meniscal tear (p = 0.007). Similar patterns were found for trabecular bone fraction (coverage p < 0.001, tear p = 0.047), trabecular thickness (coverage p = 0.03), connectivity density (coverage p = 0.002), and SMI (coverage p = 0.015). Quantitative trabecular bone evaluation emphasizes intrinsic structural differences between meniscus-covered/meniscus-uncovered tibias. Results offer insight into bone adaptation with meniscal tear and support the hypothesis that subchondral bone plate-connected bone density could be important in early subchondral bone adaptation.

MULTI-OBJECTIVE OPTIMIZATION FOR SURGICAL DRILLING OF HUMAN FEMURS: A METHODOLOGY FOR BETTER PULL-OUT STRENGTH OF FIXATION USING TAGUCHI METHOD BASED ON MEMBERSHIP FUNCTION

Drilling through bone is one of the common cutting processes involved in many of the orthopedic surgeries. In bone drilling, spindle speed, feed rate, diameter of the drill bit, drill bit geometry and method of cooling are the important parameters to influence the in-situ temperature, drill thrust force and quality characteristics of the drilled hole. Because of the selection of inappropriate drilling parameters, uncontrolled large drilling forces, continuous increase in temperature and mechanical damage to the local host bone were observed. As these adverse effects lead to poor bone–implant contact and often a revision surgery, performing a surgical drilling with optimal parameters is essential to succeed in the surgical procedure. It was observed that in addition to the variations in apparent bone density, the orientation of osteons influences the drilling thrust force and temperature in bone drilling. Ten adult cadaveric human femurs from the age group of 32–65 years were considered and drilling experiments were conducted on proximal-diaphysis, mid-diaphysis and distal-diaphysis regions in the longitudinal, radial and circumferential directions. Bone drilling with different spindle speeds (500, 1000 and 1500[Formula: see text]rpm), feed rates (40, 60 and 80[Formula: see text]mm/min), and apparent density in the range of 0.98[Formula: see text]g/cm3 to 1.98[Formula: see text]g/cm3 was investigated in this work using a 3.20[Formula: see text]mm diameter surgical drill-bit. The generation of in-situ temperature as well as thrust force at each target location was measured using [Formula: see text]-type thermocouple and Kistler[Formula: see text] dynamometer, respectively. Taguchi method based on membership function was used to optimize the drilling process. Then the efficacy of the method in reducing the in-situ temperature and thrust force, and quality of the drilled hole in respect of anatomical region and drilling direction was investigated using pull-out strength of the bone screws. Results revealed that the optimal parameters obtained from the Taguchi method based on membership function could simultaneously minimize the temperature as well as thrust force in bone drilling. The proposed method can be adopted to minimize the temperature and thrust force, and choose the best location nearest to the defect site for strong implant fixation by using CT datasets of the patient as the only input.

Numerical Simulation of Mandible Bone Remodeling under Tooth Loading: A Parametric Study

Bone adapts to the change of mechanical stimulus by bone remodeling activities. A number of numerical algorithms have been developed to model the adaptive bone remodeling under mechanical loads for orthopedic and dental applications. This paper examines the effects of several model parameters on the computed apparent bone density in mandible under normal chewing and biting forces. The density change rate was based on the strain energy density per unit mass. The algorithms used in this study containing an equilibrium zone (lazy zone) and saturated values of density change rate provides certain stability to result in convergence without discontinuous checkerboard patterns. The parametric study shows that when different boundary conditions were applied, the bone density distributions at convergence were very different, except in the vicinity of the applied loads. Compared with the effects of boundary conditions, the models are less sensitive to the choice of initial density values. Several models starting from different initial density values resulted in similar but not exactly the same bone density distribution at convergence. The results also show that higher reference value of mechanical stimulus resulted in lower average bone density at convergence. Moreover, the width of equilibrium zone did not substantially affect the average density at convergence. However, with increasing width, the areas with the highest and the lowest bone density areas were all reduced. The limitations of the models and challenges for future work were discussed for the better agreement between the computed results and the in vivo data.

Effect of local zoledronic acid administration in a rat model of posterolateral spinal fusion

Study designRandomized animal model study. PurposePosterolateral spinal fusion represents a common surgical procedure in the United States. The effect of bisphosphonate administration in these patients is poorly understood. The purpose of this study is to determine whether local administration of bisphosphonate by soaking bone autograft would affect the apparent bone density or structural properties of the fusion mass in a rat model of posterolateral spinal fusion. Methods36 Spring Dawley rats underwent L4-5 posterolateral spinal fusion with bone autograft. These rats were divided into three groups, two experimental groups and one control group. Each of the experimental groups underwent spinal fusion with morselized vertebral cortical and cancellous autograft soaked in zoledronic acid solution; one group 20 mcg/mL, another 200 mcg/mL. The control group underwent L4-5 spinal fusion with cancellous allograft soaked with saline. At 8 weeks, the rats were euthanized for analysis. Evaluations consisted of micro-CT scanning, four-point bending biomechanical testing, histology, and radiographs. ResultsBoth of the experimental groups showed statistically significant increase in apparent bone density and bone volume fraction at the fusion mass. Biomechanical measures revealed a trend for improvement in the experimental groups, but these did not reach statistical significance. ConclusionsThis data suggest that locally administered bisphosphonate medications result in increased apparent bone density and bone volume fraction at the fusion mass in posterolateral spinal fusion, and that there appear to be no deleterious consequences with regards to the stiffness or maximum load to failure of the fusion mass under flexion bending evaluation.

The Ability of Ultrasonic Backscatter Parametric Imaging to Characterize Bovine Trabecular Bone.

The ultrasonic backscatter technique holds the promise of characterizing bone density and microstructure. This paper conducts ultrasonic backscatter parametric imaging based on measurements of apparent integrated backscatter (AIB), spectral centroid shift (SCS), frequency slope of apparent backscatter (FSAB), and frequency intercept of apparent backscatter (FIAB) for representing trabecular bone mass and microstructure. We scanned 33 bovine trabecular bone samples using a 7.5 MHz focused transducer in a 20 mm × 20 mm region of interest (ROI) with a step interval of 0.05 mm. Images based on the ultrasonic backscatter parameters (i.e., AIB, SCS, FSAB, and FIAB) were constructed to compare with photographic images of the specimens as well as two-dimensional (2D) μ-CT images from approximately the same depth and location of the specimen. Similar structures and trabecular alignments can be observed among these images. Statistical analyses demonstrated that the means and standard deviations of the ultrasonic backscatter parameters exhibited significant correlations with bone density (|R| = 0.45-0.78, p < 0.01) and bone microstructure (|R| = 0.44-0.87, p < 0.001). Some bovine trabecular bone microstructure parameters were independently associated with the ultrasonic backscatter parameters (ΔR2 = 4.18%-44.45%, p < 0.05) after adjustment for bone apparent density (BAD). The results show that ultrasonic backscatter parametric imaging can provide a direct view of the trabecular microstructure and can reflect information about the density and microstructure of trabecular bone.

Development of open-cell polyurethane-based bone surrogates for biomechanical testing of pedicle screws

Artificial bones made of polyurethane are frequently used as an alternative to human bone for biomechanical testing. However, the biomechanical characteristics of these materials are often not validated against those of human bones. Thus, synthetic bone surrogates reflecting procedure-specific biomechanical properties of human bones are necessary for reliable implant design and testing. The aim of this study was to evaluate novel custom made open- and close-cell bone surrogates through morphometry assessment and pedicle screw pullout tests as an alternative to human bone for biomechanical testing. Bone surrogates created from polyurethane resin, mineral fillers and varying amounts of blowing agent were customized to various densities. Pedicle screws were manually inserted and pullout tests with a feed rate of 1 mm/min were conducted until failure. Load and displacement curves were recorded and analyzed in terms of maximum pullout forces. The resulting pullout forces of open- (1437 ± 665 N) and close-cell surrogates (951 ± 578 N) showed comparable results to human bone (1417 ± 812N) used as a reference. Furthermore, structural morphometric parameters were in accordance with human vertebral cancellous bone. In conclusion, the customized bone surrogates provide a new opportunity to design and test pedicle screws and further study the relationship between biomechanical properties and apparent density of artificial spongy bone.