A research study was carried out to examine the effects of auxin and rooting hormone on the biochemical composition of the mini clonal mulberry leaves raised using mini-clonal technique of (Morus indica L.) variety 'V1'.Two hormones, such as indole-3-butyric acid (IBA) and napthalene acetic acid (NAA), were used in the experiment. The hormones were replicated four times in each of the following concentration ranges: 1000, 2000, 3000, 4000, and 5000 ppm. Different hormone concentrations were applied to apical shoot cuttings before they were planted in the appropriate rooting medium in a greenhouse condition. The biochemical parameters analysed during the research study include leaf moisturecontent, nitrogen, phosphorous, potassium, chlorophyll-a, chlorophyll-b, carotenoid, total chlorophyll, crude protein, total carbohydrates, crude fat, total protein content present in mulberry leaves. The nutritive value of mulberry varieties depends on its biochemical constituents present in it. The results indicated that V1 mulberry mini clones have recorded better biochemical constituents and suitable for rearing of mulberry silkworms to get successful cocoon crop.