Apatite Surface Research Articles

Adsorption behavior of methacryloyloxydecyl dihydrogen phosphate on an apatite surface at neutral pH.

This study aimed to quantify the adsorption affinity of neutralized 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP-N) toward hydroxyapatite (HA) and dicalcium phosphate dihydrate (DCPD) at pH 7.0 by employing the Langmuir isotherm model. Furthermore, the effects of inorganic phosphate (Pi) and fluoride (F(-) ) ions on the adsorption of 10-MDP-N onto HA and DCPD were examined. Fixed amounts of HA and DCPD powders were suspended in different concentrations of 10-MDP-N solutions and were incubated for 18 h. Equilibrated concentrations of 10-MDP-N were measured by spectrophotometry and the adsorption affinity was estimated using the Langmuir model. Moreover, the adsorption was examined by zeta-potential analysis. The results indicated that significant Langmuir correlation was noted in both substrates, along with an increasing negative zeta-potential; however, in DCPD the correlation was less strong. The addition of 1.0 mM Pi slightly delayed the adsorption of 10-MDP-N onto both substrates, whereas 3.0 mM Pi drastically delayed adsorption onto HA but completely inhibited adsorption onto DCPD. Up to 50 ppm, F(-) enhanced the adsorption onto HA, and the adsorption plateaued at higher concentrations of F(-) , whereas no obvious influence of F(-) on the adsorption onto DCPD was noted.

Interactions of zoledronate and alendronate with hydroxyapatite and octacalciumphosphate

Event Abstract Back to Event Interactions of zoledronate and alendronate with hydroxyapatite and octacalciumphosphate Massimo Gazzano1, Adriana Bigi2, Elisa Boanini2, Katia Rubini2 and Lucia Forte2 1 CNR, ISOF, Italy 2 University of Bologna, Chemistry Dept. "G. Ciamician", Italy Introduction: Bisphosphonates (BPs) are recognized as potent inhibitors of osteoclast activity and widely used clinically for the treatment of diseases associated with conditions of bone loss. It is known that BPs act directly on osteoclasts and interfere with specific intracellular biochemical processes such as isoprenoid biosynthesis and subsequent protein prenylation to inhibit cell activity [1]. The ability to bind to bone mineral is the key of their pharmacological action as well as of the efficacy and action duration. The mineral affinity and activity of individual BPs depend on the two covalently bound sidechains, R1 and R2, attached to the central carbon atom (Scheme 1). It has been reported that zoledronate (Zol) displays a greater binding affinity than alendronate (Al) towards apatite (HA) surface [2]. Due to its important role as precursor of biological HA, octacalciumphosphate (OCP) is a good candidate for the preparation of composites for bone tissue repair. In this work we modified the classical procedure of OCP synthesis in order to prepare OCP-Al and OCP-Zol crystals at different concentration of the BPs and we demonstrate the higher affinity of Al for OCP. Materials and Methods: The synthesis of OCP, in the presence of Al and Zol, was carried out by dropwise addition of a Ca(CH3COO)2 solution into a phosphate solution. The reaction was carried out at 70°C under mechanical stirring. The precipitate was stored in contact with the mother solution for 30 min before dropwise addition of the bisphosphonate solution. Finally the precipitate was filtered, repeatedly washed with distilled water and dried. Zol and Al concentrations were in the range from 0.1 to 1.2 mM. The powders were characterized by X-Ray and SEM analysis, whereas BPs content was determined spectrophotometrically via complex formation with Fe(III) ions. Results and Discussion: By using both BPs we obtained OCP as the unique crystalline phase, as confirmed by the results of XRD analysis. Al and Zol content in the solid products increased on increasing their concentration in solution up to about 5.5% and 3.5%, respectively. Al and Zol presence during the OCP synthesis caused a reduction of the mean length of the coherently scattering domains and a reduction in the dimensions of the composite crystals compared to those of pure OCP. The data suggest different mechanisms of interaction of Zol and Al with OCP structure, which can be justified by the results of the study of calcium coordination environment. Figure 1: possible interaction of Al with the hydrated layer of OCP structure Conclusions: The different behavior of Zol and Al towards incorporation into OCP, with respect to that previously observed for HA [3],[4] suggests different mechanisms of interaction between the BPs and the calcium phosphates in the composites, which provide materials with tunable antiresorptive properties and promising potential orthopedic applications.

A mechanistic study of the interaction of water-soluble borate glass with apatite-bound heterocyclic nitrogen-containing bisphosphonates

Long-term oral and intravenous use of nitrogen-containing bisphosphonates (N-BPs) is associated with osteonecrosis of the jaw. Although N-BPs bind strongly to bone surfaces via non-covalent bonds, it is possible for extrinsic ions to dissociate bound N-BPs from mineralized bone by competitive desorption. Here, we investigate the effects and mechanism of using an ionic cocktail derived from borate bioactive glass for sequestration of heterocyclic N-BPs bound to apatite. By employing solid-state and solution-state analytical techniques, we confirmed that sequestration of N-BPs from bisphosphonate-bound apatite occurs in the presence of the borate-containing ionic cocktail. Simulations by density functional theory computations indicate that magnesium cation and borate anion are well within the extent of the risedronate or zoledronate anion to form precipitate complexes. The sequestration mechanism is due to the borate anion competing with bisphosphonates for similar electron-deficient sites on the apatite surface for binding. Thus, application of the borate-containing ionic cocktail represents a new topical lavage approach for removing apatite-bound heterocyclic N-BPs from exposed necrotic bone in bisphosphonate-related osteonecrosis of the jaw. Statement of significanceLong-term oral consumption and injections of nitrogen-containing bisphosphonates (N-BPs) may result in death of the jaw bone when there is traumatic injury to the bone tissues. To date, there is no effective treatment for such a condition. This work reported the use of an ionic cocktail derived from water-soluble borate glass microfibers to displace the most potent type of N-BPs that are bound strongly to the mineral component on bone surfaces. The mechanism responsible for such an effect has been identified to be cation-mediated complexation of borate anions with negatively-charged N-BPs, allowing them to be released from the mineral surface. This borate-containing cocktail may be developed into a novel topical rinse for removing mineral-bound N-BPs from exposed dead bone.

Active Mn species well dispersed on Ca2+ enriched apatite for total oxidation of toluene

Hydroxyapatite (Hap)-supported manganese oxide catalysts (10wt% Mn based on MnO2) were prepared by two different routes, from Mn nitrate (Nit) and Mn acetate (Ac) precursors. The catalyst precursors were calcined at 400°C for 4h to get the final MnNit-Hap and MnAc-Hap catalysts. Elemental analysis, XRD, SEM, HRTEM, N2 adsorption experiments, FT-IR, temperature programmed reduction (H2-TPR), X-ray photoelectron spectroscopy (XPS) and ToF-SIMS characterization methods were used to identify the Mn species and their environment. The overall characterization studies indicated that the nature of the counter-ion played a significant role in the speciation and dispersion of manganese species in Hap although the Mn AOS in both cases is around 3. The nitrate Mn precursor allowed to get after calcination at 400°C well dispersed and easily reduced active Mnn+ (n+≥3) oxidized species on a Ca2+ enriched hydroxyapatite surface, likely in a polymeric form or/and in small MnxOy crystallites, while part of Mn in the form of Mn2+ ions incorporated into the Hap substituting Ca2+ at the apatite surface. By opposition the acetate Mn precursor primarily results in large Mn3O4 oxide nanocrystals along with an amorphous Mn5O8 phase and Hap incorporated Mn2+ ions.The total oxidation of toluene was investigated on these new catalysts and the catalytic performances were compared to those of a reference 10wt%Mn on alumina (Mn–Al). It is found that the nature of the support plays a significant role. Indeed the different AOS of Mn (about 3 for supported Hap; ≈4 for supported alumina catalyst) showed that the Mn active phases are different. The best performances were achieved on the MnNit-Hap catalyst with the complete conversion of toluene at 220°C. The Mn oxidized species well dispersed on Ca2+ enriched apatite using a strong oxidizing counter-ion as NO3− in the Mn precursor were responsible for optimal toluene conversion. In that case it was found that the incorporation of part of NO3− in the hydroxyapatite promoted the surface Ca2+ enrichment of Hap by the incorporation of Mn2+ into the Hap support.

Adsorption of nucleotides on biomimetic apatite: The case of adenosine 5′ monophosphate (AMP)

This work investigates the interaction between the nucleotide adenosine 5′ monophosphate molecule (AMP) and a biomimetic nanocrystalline carbonated apatite as a model for bone mineral. The analogy of the apatite phase used in this work with biological apatite was first pointed out by complementary techniques. AMP adsorption isotherms were then investigated. Obtained data were fitted to a Sips isotherm with an exponent greater than one suggesting positive cooperativity among adsorbed molecules. The data were compared to a previous study relative to the adsorption of another nucleotide, cytidine monophosphate (CMP) onto a similar substrate, evidencing some effect of the chemical nature of the nucleic base. An enhanced adsorption was observed under acidic (pH 6) conditions as opposed to pH 7.4, which parallels the case of DNA adsorption on biomimetic apatite. An estimated standard Gibbs free energy associated to the adsorption process (ΔG°ads≅−22kJ/mol) intermediate between “physisorption” and “chemisorption” was found. The analysis of the solids after adsorption pointed to the preservation of the main characteristics of the apatite substrate but shifts or enhancements of Raman bands attributed to AMP showed the existence of chemical interactions involving both the phosphate and adenine parts of AMP. This contribution adds to the works conducted in view of better understanding the interaction of DNA/RNA and their constitutive nucleotides and the surface of biomimetic apatites. It could prove helpful in disciplines such as bone diagenesis (DNA/apatite interface in aged bones) or nanomedicine (setup of DNA- or RNA-loaded apatite systems). Also, the adsorption of nucleic acids on minerals like apatites could have played a role in the preservation of such biomolecules in the varying conditions known to exist at the origin of life on Earth, underlining the importance of dedicated adsorption studies.

Spectroscopic characterisation of hydroxyapatite and nanocrystalline apatite with grafted aminopropyltriethoxysilane: nature of silane–surface interaction

Heterogenised homogeneous catalysis is commonly performed with molecular catalysts grafted on solids via adsorption or via a covalent molecular link. Covalent grafting of organic groups on solid supports is usually carried out by silylation, using functionalised trialkoxysilanes. Among these solids supports, very few studies have been published on apatites. In the present work, aminopropyltriethoxysilane (APTES) grafting was performed in toluene on different apatitic supports: crystallised stoichiometric hydroxyapatites differing by the drying method, freeze-dried (HAP) and dried at 100 °C (HAPD), and a nanocrystalline apatite. All materials were fully characterised, before and after grafting, for better understanding of the nature of the alkoxysilane/surface interaction. The data show a clear competition between the covalent grafting of APTES and its polycondensation reaction, depending on the nature of the solid support surface. Silylation is accompanied by APTES covalent grafting to oxygen atom of the hydroxyl groups of the apatitic structure and/or of the OH− species that are present on the surface hydrated layer. This work clarifies the nature of silane grafting onto selected apatitic surfaces and especially the influence of the composition and properties of the apatitic surfaces on the process of silylation.

A mixed collector system for phosphate flotation

Flotation has been used in industry for more than a half century as the primary technique for upgrading phosphate. While the flotation of phosphate was inefficient when oleic acid was used alone as a collector, therefore a mixed collector of oleic acid (HOl), linoleic acid (LA) and linolenic acid (LNA) was employed to improve the recovery of phosphate flotation. The batch flotation results showed that the optimal composition of the mixed collector was 54wt.% HOl, 36wt.% LA and 10wt.% LNA. Additionally, the effect of pH on the mixed collector application was studied while considering the surface tension, contact angle and micro-flotation. The results showed that the mixed collector should be used at a pH of 9.5. Above a pH of 9.5, the adsorption of fatty acids dimers on the apatite surface hindered phosphate flotation. The influence of the mixed collector assembly on apatite flotation was also investigated. It was demonstrated that due to its low critical micelle concentration, a sufficiently hydrophobic apatite surface could be generated at a collector concentration of 60mg/L. In addition, zeta potential experiments suggested that collector adsorption was governed by chemisorption. FTIR and XPS spectra studies further indicated that the chemical reaction involved the carboxyl groups of fatty acids and Ca species at the apatite surface for each fatty acid in the mixed collector.

Sol–gel synthesis and in vitro bioactivity of copper and zinc-doped silicate bioactive glasses and glass-ceramics

Metal doping of bioactive glasses based on ternary 60SiO2–36CaO–4P2O5 (58S) and quaternary 60SiO2–25CaO–11Na2O–4P2O5 (NaBG) mol% compositions synthesized using a sol–gel process was analyzed. In particular, the effect of incorporating 1, 5 and 10 mol% of CuO and ZnO (replacing equivalent quantities of CaO) on the texture, in vitro bioactivity, and cytocompatibility of these materials was evaluated. Our results showed that the addition of metal ions can modulate the textural property of the matrix and its crystal structure. Regarding the bioactivity, after soaking in simulated body fluid (SBF) undoped 58S and NaBG glasses developed an apatite surface layer that was reduced in the doped glasses depending on the type of metal and its concentration with Zn displaying the largest inhibitions. Both the ion release from samples and the ion adsorption from the medium depended on the type of matrix with 58S glasses showing the highest values. Pure NaBG glass was more cytocompatible to osteoblast-like cells (SaOS-2) than pure 58S glass as tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The incorporation of metal ions decreased the cytocompatibility of the glasses depending on their concentration and on the glass matrix doped. Our results show that by changing the glass composition and by adding Cu or Zn, bioactive materials with different textures, bioactivity and cytocompatibility can be synthesized.

Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms.

Biofilms are highly structured microbial communities that are enmeshed in a self-produced extracellular matrix. Within the complex oral microbiome, Streptococcus mutans is a major producer of extracellular polymeric substances including exopolysaccharides (EPS), eDNA, and lipoteichoic acid (LTA). EPS produced by S. mutans-derived exoenzymes promote local accumulation of microbes on the teeth, while forming a spatially heterogeneous and diffusion-limiting matrix that protects embedded bacteria. The EPS-rich matrix provides mechanical stability/cohesiveness and facilitates the creation of highly acidic microenvironments, which are critical for the pathogenesis of dental caries. In parallel, S. mutans also releases eDNA and LTA, which can contribute with matrix development. eDNA enhances EPS (glucan) synthesis locally, increasing the adhesion of S. mutans to saliva-coated apatitic surfaces and the assembly of highly cohesive biofilms. eDNA and other extracellular substances, acting in concert with EPS, may impact the functional properties of the matrix and the virulence of cariogenic biofilms. Enhanced understanding about the assembly principles of the matrix may lead to efficacious approaches to control biofilm-related diseases.

Tetracycline-loaded biomimetic apatite: an adsorption study.

Biomimetic apatites are appealing compounds for the elaboration of bioactive bone-repair scaffolds due to their intrinsic similarity to bone mineral. Bone surgeries are however often heavy procedures, and the infiltration of pathogens may not be totally avoided. To prevent their development, systemic antibiotic prophylaxis is widespread but does not specifically target surgical sites and involves doses not always optimized. A relevant alternative is a preliminary functionalization by an infection-fighting agent. In this work, we investigated from a physicochemical viewpoint the association of a wide-spectrum antibiotic, tetracycline (TC), and a biomimetic nanocrystalline apatite previously characterized. TC adsorption kinetics and isotherm were thoroughly explored. Kinetic data were fitted to various models (pseudo-first-order, pseudo-second-order, general kinetic model of order n, Elovich, double-exponential, and purely diffusive models). The best fit was found for a double-exponential kinetic model or with a decimal reaction order of 1.4, highlighting a complex process with such TC molecules which do not expose high-affinity end groups for the surface of apatite. The adsorption isotherm was perfectly fitted to the Sips (Langmuir-Freundlich) model, while other models failed to describe it, and the Sips exponent greater than unity (1.08) suggested a joint impact of surface heterogeneity and positive cooperativity between adsorbed molecules. Finally, preliminary insights on TC release from pelletized nanocrystalline apatite, in aqueous medium and neutral pH, were obtained using a recirculation cell, indicating a release profile mainly following a Higuchi-like diffusion-limited rate. This work is intended to shed more light on the interaction between polar molecules not exhibiting high-affinity end groups and biomimetic apatites and is a starting point in view of the elaboration of biomimetic apatite-based bone scaffolds functionalized with polar organic drugs for a local delivery.

Visualization of structural organization of ventral membranes of sheared-open resorbing osteoclasts attached to apatite pellets

Osteoclasts are highly polarized cells from both morphological and functional points of view. Using quick-freeze, rotary-replication methods combined with cell-shearing, we clarified the variability of cytoplasmic surface of the polarized membranes of osteoclasts seeded on apatite. As to the organization of actin filaments and clathrin sheets, we confirmed almost the same ventral membrane specializations of osteoclasts on apatite as seen on glass plates. The organized actin filaments and membrane-associated particles supported the ruffled border membranes. Inside the actin sealing zone, membrane specializations were not always occupied with the ruffled border but also with other types of membranes. Some osteoclasts formed an actin ring but lacked the ruffled border projections. We report a unique and distinctive membrane modification of apatite-attached osteoclasts, i.e., the presence of dense aggregates of membrane-associated particles and related structures not found in the osteoclasts seeded on glass plates. Actin filament polarity in the podosomes was determined by decoration with myosin S1. The actin filament polarity within podosome appears to be oriented predominantly with its barbed ends toward the core, whereas the interconnecting F-actin appears to be mixed oriented. Two different types of clathrin plaques displayed different distributions: clathrin-dependent endocytosis was observed in the ruffled border regions, whereas flat clathrin sheets were found in the leading edge of lamellipodia and near podosomes. The clathrin sheets adhered to the apatite surface tightly on the ventral membranes overlaying the resorption lacunae. All these membrane specializations as mentioned above may indicate the functional variability of osteoclasts seeded on apatite.

Influence of dissolution medium pH on ion release and apatite formation of Bioglass® 45S5

Bioactive glasses, particularly Bioglass® 45S5, have been used to clinically regenerate human bone since the mid-1980׳s, owing to their ability to degrade in physiological solutions, release ions and form an apatite surface layer, which cells adhere to and proliferate on. Although low pH conditions do occur in the human body, e.g. during bacterial infections, in vitro dissolution experiments are usually performed at a physiological pH of 7.3 exclusively. Here, we investigated the dissolution behaviour of 45S5 at low pH (5) and high pH (9) in addition to pH 7.3. The results show that ion release occurs significantly faster at low pH, resulting in significantly faster apatite formation (3h vs. 6h at pH 7.3). By contrast, at pH 9 low ion exchange rates were observed, resulting in no significant apatite formation during the time period studied. Results suggest that low pH caused by bacterial infection is unlikely to inhibit apatite formation and, thus, bioactive glass clinical performance.

Dual-functioning phage-derived peptides encourage human bone marrow cell-specific attachment to mineralized biomaterials

Cell instructive mineralized biomaterials are a promising alternative to conventional auto-, allo-, and xenograft therapies for the reconstruction of critical sized defects. Extracellular matrix proteins, peptide domains, and functional motifs demonstrating cell and mineral binding activity have been used to improve cell attachment. However, these strategies vary in their tissue regeneration outcomes due to lack of specificity to both regenerative cell populations and the material substrates. In order to mediate cell-specific interactions on apatite surfaces, we identified peptide sequences with high affinity towards apatite (VTKHLNQISQSY, VTK) and clonally derived human bone marrow stromal cells (DPIYALSWSGMA, DPI) using phage display. The primary aims of this study were to measure apatite binding affinity, human bone marrow stromal cell (hBMSC) adhesion strength, and peptide specificity to hBMSCs when the apatite and cell-specific peptides are combined into a dual-functioning peptide. To assess binding affinity to hydroxyapatite (HA), binding isotherms were constructed and peptide binding affinity (K1) determined. HBMSC, MC3T3 and mouse dermal fibroblast (MDF) adhesion strength on biomimetic apatite functionalized with single- and dual-functioning peptide sequences were evaluated using a centrifugation assay. DPI-VTK had the highest binding strength towards hBMSCs (p < 0.01). DPI-VTK, while promoting strong initial attachment to hBMSCs, did not encourage strong adhesions to MC3T3s or fibroblasts (p < 0.01). Taken together, phage display is a promising strategy to identify preferential cell and material binding peptide sequences that can tether specific cell populations onto specific biomaterial chemistries.

Up-to-date approaches to studying adsorption of fatty-acid collecting agents at apatite and shtaffelite ore minerals

The morphology and spread of micro- and nano-formations on the surface of apatite and shtaffelite ore minerals (apatite, shtaffelite and calcite) due to adsorption of fatty-acid collecting agents have been studied using the methods of optical, laser confocal scanning, analytical electron and atomic force microscopy. The Fourier-transform IR spectroscopy has shown that fatty-acid collecting agents are adsorbed at the surface of apatite, shtaffelite and calcite in a mixed ion-molecule form; the ion form of attachment prevails for calcite.

Bioactivity Assessment of Niobate Apatite

Hydroxyapatite is a bioceramic material of great interest for use as bone substitute because of its similarity with the composition of biological apatite. Cationic and anionic substitutions in the apatite structure have been made in order to optimize the synthesis and accelerate the process of bone repair. In the present study, niobate apatite was synthesized by a patented aqueous precipitation method. The bioactivity of the samples was assessed by X-ray diffraction analyses (XRD), energy dispersive X-ray spectroscopy (EDS) and scanning electron microscopy with field emission gun (FEG-SEM; FEI Quanta FEG 250) in the samples before and after an incubation period in simulated body fluid. The results showed that after 3 days a bone-like apatite coating was formed onto the niobate apatite surface. A peculiar morphology comprised by nanosized wires was also observed on the niobate apatite surface.

The role of chemistry and pH of solid surfaces for specific adsorption of biomolecules in solution—accurate computational models and experiment

Adsorption of biomolecules and polymers to inorganic nanostructures plays a major role in the design of novel materials and therapeutics. The behavior of flexible molecules on solid surfaces at a scale of 1–1000 nm remains difficult and expensive to monitor using current laboratory techniques, while playing a critical role in energy conversion and composite materials as well as in understanding the origin of diseases. Approaches to implement key surface features and pH in molecular models of solids are explained, and distinct mechanisms of peptide recognition on metal nanostructures, silica and apatite surfaces in solution are described as illustrative examples. The influence of surface energies, specific surface features and protonation states on the structure of aqueous interfaces and selective biomolecular adsorption is found to be critical, comparable to the well-known influence of the charge state and pH of proteins and surfactants on their conformations and assembly. The representation of such details in molecular models according to experimental data and available chemical knowledge enables accurate simulations of unknown complex interfaces in atomic resolution in quantitative agreement with independent experimental measurements. In this context, the benefits of a uniform force field for all material classes and of a mineral surface structure database are discussed.

Adsorption on apatitic calcium phosphates for drug delivery: interaction with bisphosphonate molecules.

Bisphosphonates (BPs) are well established as an important class of drugs for the treatment and prevention of several bone disorders including osteoporosis. This work investigated the interaction of two bisphosphonates, risedronate and tiludronate, with several apatitic supports, a well-crystallised hydroxyapatite (HA) and nanocrystalline apatites with varying maturation times, chemical composition and surface characteristics. The purpose was to fully understand the adsorption mechanism and desorption process, by the evaluation of the effect of several physicochemical parameters (temperature, pH and concentration of calcium and phosphate ions). Whatever the nature of the BP and the structure and composition of the apatite, the adsorption of such anti-resorptive agents can be well described as an ion exchange-reaction between phosphates species on the apatitic surface and BP molecules in solution. However, the parameters of adsorption can vary depending on the physicochemical conditions of the adsorption reaction. In addition, the structure and composition of the apatitic surface also influence the adsorption properties. Finally, BPs molecules are slowly released from apatitic supports, because most of the adsorbed molecules are irreversibly bound and not spontaneously released by dilution or simple washing. Moreover, similar to their adsorption, the release of bisphosphonates is strongly affected not only by the chemical properties of the molecule, but also by the chemical and structural characteristics of the apatitic substrates. The understanding of the adsorption and release processes provides fundamental tools for the development of drug delivery systems using apatite materials.

Infrared, Raman and NMR investigations of risedronate adsorption on nanocrystalline apatites

The aim of the current work was to study the physico-chemical interactions of a bisphosphonate molecule, risedronate, with a well-characterised synthetic nanocrystalline apatite (NCA) as a model bone mineral. We adopted a global approach, using complementary physico-chemical techniques such as FTIR, RAMAN and NMR spectroscopies in order to learn more about the interaction process of risedronate with the apatitic surface. The results obtained suggest that risedronate adsorption corresponds to an ion substitution reaction with phosphate ions occurring at the crystal surface. This mechanism explains the greater amount adsorbed (N) for NCA, compared to well crystallised stoichiometric hydroxyapatite, attributable to the well-developed hydrated layer at the surface of the nanocrystals. However, most calcium ions remain attached to the solid phase and the formation of insoluble risedronate calcium salts must also be considered as a competitive reaction to the adsorption. Thus a calcium risedronate salt was synthesised and fully characterised for comparison to the solids after adsorption. Following spectroscopic results, it can be concluded that a strong interaction was established between risedronate ions and calcium ions at the apatitic surface. However, under these experimental conditions there is no nucleation of a distinct calcium risedronate salt and the apatite crystals retain their integrity.

Investigating in vitro bioactivity and magnetic properties of the ferrimagnetic bioactive glass–ceramic fabricated using soda-lime–silica waste glass

The main purpose of the current research is the production and characterization of a ferrimagnetic bioactive glass–ceramic prepared through the solid-state reaction method using soda-lime–silica waste glass as the main raw material. In comparison with the conventional route, that is, the melt-quenching and subsequent heat treatment, the present work is an economical technique. Structural, thermal and magnetic properties of the samples were examined by X-ray diffraction (XRD), differential thermal analysis (DTA) and vibrating sample magnetometer (VSM). The in vitro test was utilized to assess the bioactivity level of the samples by Hanks' solution as simulated body fluid (SBF). The apatite surface layer formation was examined by the scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The calcium ion concentration in the solutions was measured by atomic absorption spectroscopy (AAS). VSM results revealed that with the addition of 5–20wt% strontium hexaferrite to bioactive glass–ceramics, the ferrimagnetic bioactive glass–ceramics with hysteresis losses between 7024 and 75,852erg/g were obtained. The in vitro test showed that the onset formation time of hydroxyapatite layer on the surface of the samples was 14 days and after 30 days, this layer was completed.

Comparative Study of Surface Functionalization of Hydroxyapatite by One‐pot Grafting of Organophosphonates Species

AbstractThe ability to graft apatite surface by specific ligand fields creates powerful new technology applications. Thus, grafted organophosphonate can provide an effective route to modify the surface properties of apatite materials. The ability to decorate the apatite surface with the desired functionality requires covalent attachment to solid interface; most commonly achieved using organophosphonate self‐assembly. The current study shows that various phosphonates can react with calcium ions and control the formation of the porous apatite with high surface areas as well as the porosity. The structural and surface properties are given some importance in this review.