The traditional sinoaortic denervation (SAD) technique in rats includes aortic and carotid baroreceptors denervation, but also leads to concomitant denervation of the carotid chemoreceptors. Here we report a new technique to denervate the carotid and aortic baroreceptors, selectively, preserving the carotid chemoreceptors intact. Wistar rats were subjected to selective aortic and carotid baroreceptor denervation sparing the carotid chemoreceptors intact (BAROS-X), or sham surgery (SHAM). The animals were also implanted with femoral arterial and venous catheter for arterial pressure recording and drug administration. In unanesthetized, freely moving rats, baroreflex or peripheral chemoreflex activation was elicited by intravenous injection of phenylephrine or potassium cyanide (KCN), respectively. Phenylephrine caused a significant hypertensive response in SHAM (Δ = 46 ± 3 mmHg, P<0.001) and BAROS-X (Δ = 38 ± 3 mmHg, P<0.001) combined with a reflex bradycardia in SHAM (Δ = -54 ± 11 bpm, P<0.001) but no change in heart rate in BAROS-X subjects (Δ = -5 ± 2 bpm, ns); confirming the absence of aortic and carotid baroreceptors in BAROS-X rats. KCN elicited significant hypertensive response in SHAM (44 ± 6 mmHg, P<0.001) and BAROS-X (39 ± 5 mmHg, P<0.001) subjects followed by bradycardic response in SHAM (-88 ± 17 bpm, P<0.001) and BAROX-S (-103 ± 23 bpm, P<0.01) rats; confirming that the carotid chemoreceptors were intact in both groups. These data provide support to a new surgical technique that allows working with conscious rats exhibiting selective inactivation of the aortic and carotid baroreceptors, combined with intact carotid chemoreceptors.