Objectives: This study aimed to evaluate the levels of stress, anxiety, and depression in patients with chronic kidney disease (CKD) and examine their impact on quality of life (QOL). Material and Methods: The study conducted at KIMS-Saveera in Anantapur focused on 400 individuals diagnosed with CKD. To gather information, an online survey using Google Forms was employed. This survey included questions about health, as well as assessments using the Depression, Anxiety, and Stress Scale-21 to understand mental well-being and the kidney-related QOL scale to evaluate the QOL related to kidney disease. Results: The survey revealed that 300 males and 100 females participated in the study, and most of the participants had moderate levels of depression (41.3), anxiety (37.5), and stress (31.7). Notably, a significant negative correlation was observed between age and psychological health (PHC) (r = −0.198, n = 400, P = 0.004). Conclusion: This study revealed that stress, anxiety, and depression moderately impact the QOL for CKD patients. The findings underscore the importance of considering mental health factors in the overall care of individuals and emphasizing a holistic approach to address both physical and emotional well-being.