Antiviral Coatings Research Articles

Science-Based Strategies of Antiviral Coatings with Viricidal Properties for the COVID-19 Like Pandemics.

The worldwide, extraordinary outbreak of coronavirus pandemic (i.e., COVID-19) and other emerging viral expansions have drawn particular interest to the design and development of novel antiviral, and viricidal, agents, with a broad-spectrum of antiviral activity. The current indispensable challenge lies in the development of universal virus repudiation systems that are reusable, and capable of inactivating pathogens, thus reducing risk of infection and transmission. In this review, science-based methods, mechanisms, and procedures, which are implemented in obtaining resultant antiviral coated substrates, used in the destruction of the strains of the different viruses, are reviewed. The constituent antiviral members are classified into a few broad groups, such as polymeric materials, metal ions/metal oxides, and functional nanomaterials, based on the type of materials used at the virus contamination sites. The action mode against enveloped viruses was depicted to vindicate the antiviral mechanism. We also disclose hypothesized strategies for development of a universal and reusable virus deactivation system against the emerging COVID-19. In the surge of the current, alarming scenario of SARS-CoV-2 infections, there is a great necessity for developing highly-innovative antiviral agents to work against the viruses. We hypothesize that some of the antiviral coatings discussed here could exert an inhibitive effect on COVID-19, indicated by the results that the coatings succeeded in obtaining against other enveloped viruses. Consequently, the coatings need to be tested and authenticated, to fabricate a wide range of coated antiviral products such as masks, gowns, surgical drapes, textiles, high-touch surfaces, and other personal protective equipment, aimed at extrication from the COVID-19 pandemic.

Can Nanotechnology and Materials Science Help the Fight against SARS-CoV-2?

Since 2004, we have been developing nanomaterials with antimicrobial properties, the so-called nanoantimicrobials. When the coronavirus disease 2019 (COVID-19) emerged, we started investigating new and challenging routes to nanoantivirals. The two fields have some important points of contact. We would like to share with the readership our vision of the role a (nano)materials scientist can play in the fight against the COVID-19 pandemic. As researchers specifically working on surfaces and nanomaterials, in this letter we underline the importance of nanomaterial-based technological solutions in several aspects of the fight against the virus. While great resources are understandably being dedicated to treatment and diagnosis, more efforts could be dedicated to limit the virus spread. Increasing the efficacy of personal protection equipment, developing synergistic antiviral coatings, are only two of the cases discussed. This is not the first nor the last pandemic: our nanomaterials community may offer several technological solutions to challenge the ongoing and future global health emergencies. Readers’ feedback and suggestions are warmly encouraged.

Membrane Scaffolds Silver Nanoparticles As a New Platform of Antiviral Personal Protective Equipment for COVID-19

The coronavirus disease 2019 (COVID-19) emerged as a worldwide challenge to researchers and scientists. All research communities are working relentlessly in their labs hoping to find cure for the current COVID-19 pandemic. Developing a new vaccine for COVID-19 is not down on the road, it is a long process that needs numerous clinical trials. Moving the battle out of the body requires personal protective equipment (PPE), particularly masks and gloves. Together, they will limit spread of the virus, whether by reinforcing the PPE and developing synergistic antiviral coatings or finding a cure and vaccine to COVID-19.Virus infection is one of the major global health challenges. In the meantime, several antiviral agents have been under testing to assess their efficacy as potential therapies for COVID-19. The viral resistance and the side effects associated with antiviral agents are adding more challenges, which resulted in lagging of their effectiveness as antiviral treatment. This opens the door for the development of safe and potent alternative antiviral drugs. In the current study, silver nanoparticles loaded fiber mat have emerged as a new antiviral platform in terms of their capability to deactivate a wide array of viruses. Silver nanoparticles have been studied exhaustively for their antimicrobial activity against wide-ranging of bacteria. Besides they have also shown promising activity versus several types of viruses including hepatitis B, human immunodeficiency virus (HIV), herpes simplex, monkey pox, and respiratory syncytial virus. Silver nanoparticles provide an alternative route as a new antiviral therapy. Interestingly, silver metals have the power to attack multiple targets in the virus, which results in diminishing its possibility to develop resistance as compared to imitative antivirals.Considering the ongoing challenge, our lab has been preparing silver nanoparticles loaded on filter papers, cellulose membrane, polymer dressings, and cotton fabrics. These new generation of fiber mats will be fully characterized and tested against several viruses. Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS) will be used to determine the morphology of our fibers and measure the particle size of the embedded nanoparticles.

Poly-γ-glutamate-based Materials for Multiple Infection Prophylaxis Possessing Versatile Coating Performance.

Poly-γ-glutamate (PGA) possesses a nylon-like backbone and polyacrylate-like carboxyl groups, and shows an extraordinary solubility in water. In this study, the effective synthesis and structural analysis of some water-insoluble PGA ion-complexes (PGAICs) using cationic surfactants, hexadecylpyridinium (HDP), dodecylpyridinium, benzalkonium and benzetonium, were examined. We demonstrated their spontaneous coating performance to the surfaces of different materials (i.e., plastics, metals, and ceramics) as potent anti-staphylococcal and anti-Candida agents. The tests against Staphylococcus aureus revealed that, regardless of a variety of materials, PGAICs maintained surface antimicrobial activity, even after the water-soaking treatment, whereas those against Candida albicans indicated that, among PGAICs, PGA/HDP complex is most useful as an anti-fungal agent because of its coating stability. Moreover, the log reduction values against Influenza A and B viruses of PGA/HDP-coated surfaces were estimated to be 5.4 and 3.2, respectively, suggesting that it can be dramatically suppressed the infection of influenza. This is to our knowledge the first observation of PGA-based antiviral coatings.

Preparation, application and testing of permanent antibacterial and antiviral coatings

Protocols for the synthesis of the microbicidal polycation N,N-dodecyl,methyl-polyethylenimine and coating (painting) of glass slides with this polycation's butanol solution are described. Subsequently detailed are the procedures for validating that the resultant coated slides are essentially 100% lethal to the human bacterial pathogens, Staphylococcus aureus and Escherichia coli, as well as to two common strains of influenza virus. The time required to prepare and apply the cationic polymer and to test its microbicidal efficiency is conservatively estimated to be <4 weeks.

Biology of adenovirus and its use as a vector for gene therapy.

ADENOVIRUSES WERE FIRST DISCOVERED half a century ago by Rowe and colleagues, who were trying to culture adenoid tissue in the laboratory (Rowe et al., 1953). Since that time, nonhuman adenoviruses have been isolated from a number of species including chimpanzees, pig, mouse, and dog, as well as other mammalian and avian species (Shenk, 1996). Although human adenoviruses cause significant levels of respiratory, ocular, and gastrointestinal disease, they have been the object of intense study over the years mainly as a model system for basic eukaryotic cellular processes such as transcription, RNA processing, DNA replication, translation, and oncogenesis. Early on, it was shown that the virus could recombine during growth in culture (Lewis et al., 1966a,b; Pierce et al., 1968; Lewis and Rowe, 1970), ultimately setting the stage for the use of the virus as a vector for gene delivery into cells, animals, and humans. Many features of adenovirus make it well suited for gene delivery, including the ability to grow recombinant viruses to high titers, a relatively high capacity for transgene insertion, and efficient transduction of both quiescent and actively dividing cells, usually without incorporation of viral DNA into the host cell genome. These characteristics, as well as the development of many methods for manipulating the viral genome, have made adenovirus a popular choice as a gene delivery vehicle. This is evidenced by the fact that adenovirus is currently being used in roughly one-quarter of all gene therapy clinical trials, making it second only to the use of retroviral vectors (Journal of Genetic Medicine Website, www. wiley.co.uk/genmed/clinical). In this review, we summarize our current understanding of the life cycle of the human adenoviruses and then discuss how the structure and function of viral genes impact the use of the virus as a gene delivery vehicle. Readers desiring a more comprehensive coverage of the biology of the virus are directed to the volumes edited by Doerfler and Bohm (2003, 2004). VIRUS STRUCTURE