Antiseptic Preparation Research Articles

FORMULASI DAN UJI MUTU FISIK SEDIAAN GEL EKSTRAK BUNGA TELANG (Clitoria ternatea L.) SEBAGAI ANTISEPTIK TANGAN

Hands are one of the media for microbial growth, dirty hands become a breeding ground for microbes such as Staphylococcus aureus and Escherichia coli or other bacteria. How to maintain hand hygiene is to wash your hands with soap or use an antiseptic (hand sanitizer). Antiseptic preparations are substances that have been widely used to inhibit the growth and kill microorganisms that exist around the body surface, for example on the palms of the hands that often come into contact with the surface of an object. One of the natural ingredients that has the potential to be developed as a hand antiseptic is telang flower (Clitoria ternatea). This research will formulate antiseptic gel preparations from telang flower extract, and how to test the physical quality of antiseptic gel preparations from telang flower extract. The method used for the extraction process here uses the maceration method, to maintain the antimicrobial compounds contained in the telang flower, followed by testing the physical characteristics and quality of the telang flower gel preparation, including, Organoleptic Test, pH test, Viscosity test, Homogeneity test, Spreadability test, adhesion test. After testing the characteristics and physical quality of the gel preparations of telang flower extract, the results were quite optimal, namely with a pH value in the range of 4.26-4.41 according to skin pH, and for viscosity at a value of 64% - 33%, in accordance with the characteristics of a good gel. Telang flower extract can be made and formulated as an antiseptic hand gel preparation. Variations in the concentration of the extract used greatly affect the characteristics of the gel preparation, starting from pH, viscosity, adhesion, and dispersion. The higher the concentration of the extract used, the lower the viscosity value, and the lower the pH value. The pH value and viscosity value for all formulas were in accordance with the skin parameter criteria, namely 3.5-6.5. Based on research conducted, Formula 3 using a concentration of 0.5 has. the most optimal gel preparation

Pilot clinical trial: propidium monoazide PCR quantifies reduction of the viable bacterial load after antiseptic preparation of canine oral mucosa.

A pilot clinical study to evaluate the use of propidium monoazide PCR (PMA-PCR) in quantifying a reduction of bacterial load after antiseptic use on the canine oral mucosa and skin, comparison of quantitative PCR (qPCR) to PMA-PCR, and comparison of patterns seen between PCR methods and bacterial culture. Client-owned dogs (n = 10) undergoing general anesthesia and intravenous catheter placement. The oral mucosa and antebrachial skin of each dog underwent swabs for culture, qPCR, and PMA-PCR before and after antiseptic preparation of each site. Reduction in bacterial load between sampling times was evaluated for each quantification method. All testing methods found a significant decrease in bacterial load from oral mucosa after antiseptic preparation (culture P = .0020, qPCR P = .0039, PMA-PCR P = .0039). PMA-PCR had a significantly greater reduction of bacterial load after preparation than qPCR (P = .0494). Only culture detected a significant reduction after preparation of the skin (culture P = .0039, qPCR P = .3125, PMA-PCR P = .0703). PMA-PCR was able to quantify a reduction of bacterial load after antiseptic preparation of the high-bacterial load environment, with a pattern similar to that of culture, and was more specific than qPCR for detecting viable bacterial load. The results of this study support the use of PMA-PCR for antiseptic effectiveness studies performed on a high-bacterial load environment, such as canine oral mucosa.

The dermal toxicity of an antiseptic preparation as determined by the different research methods

The article presents the results of the study of acute and subacute skin toxicity of an antiseptic ointment based on ichthyol. The research was conducted using the classic method and according to the methodology of the Organization for Economic Cooperation and Development (OECD No. 410). The study of the dermal toxicity of the product under study by the classic method showed that long-term use of the drug in animals of the experimental groups did not cause significant changes in the concentration of hemoglobin, the number of erythrocytes, leukocytes, the value of hematocrit, the average concentration of hemoglobin in the erythrocyte (MCHC), the average volume of the erythrocyte (MCV), the average content of hemoglobin in the erythrocyte (MCH) and the number of platelets, the content of total protein, the level of creatinine and the activity of AST. The study of skin toxicity of the product under study according to the OECD method No. 410 showed that the number of erythrocytes increased in the animals of the I, II, and III research groups, respectively, by – 10.7 (Р < 0.01), 6.0 (Р < 0.05), and 7.5 % (Р < 0.05). At the same time, the concentration of hemoglobin did not change. In addition, in the animals of the I and II experimental groups, a tendency towards an increase in hematocrit was established. In the animals of the I, II, and III experimental groups, changes in the erythrocyte index were noted; in particular, a probable decrease in the average content of hemoglobin in the erythrocyte (MCH) was established, respectively by – 5.2 (P < 0.05), 5.2 (P < 0.01) and 7.1 % (P < 0.001) of the average erythrocyte volume (MCV), respectively by – 6.9 (P < 0.01), 5.5 % (P < 0.01) and 7.2 % (P < 0.001). The use of the drug in a therapeutic dose caused a slight decrease in the level of total protein, urea, and creatinine against a slight increase in the activity of AST and ALT. At the same time, the animals of the II and III research groups, which received the drug under investigation at five times and ten times the therapeutic dose, noted an increase in the level of urea, respectively, by – 3.3 and 8.5 %, and creatinine, respectively, by – 1.9 and 8.0 % (P < 0.05), the activity of AST, respectively, by – 2.3 and 3.2 %, and ALT, respectively, by – 10.4 (P < 0.05) and 13.4 % (Р < 0.05) compared to the values of animals of the control group.

SG-APSIC1092: Bundled preoperative preparation reduced surgical-site infections

Objectives: We aimed to reduce the overall surgical-site infection (SSI) rate to 0.2%. Methods: A new checklist protocol was developed based on the APSIC guidelines. The bundle for preoperative preparation was implemented: adequate preoperative bathing, proper time of hand-forearm washing, and sufficient contact time of antiseptic application. The compliance rate was monitored with a weekly control chart from December 2019 to November 2020. Results: In total, 9,995 cases were operated at Narasuan University Hospital (NUH) in 2020, classified by surgical wound type as follows: clean wound, 62.6%; clean-contaminated wound, 32.1%; contaminated wound, 0.8%; and dirty wound, 4.5%. According to surgical wound type, the mean compliance with preoperative bathing was 68.22% for clean wounds, 68.33% for clean-contaminated wounds, and 34.82% for contaminated wounds. Hand hygiene preparation compliance was higher for clean wound surgeries (mean, 94.01%) and clean-contaminated wound surgeries (mean, 95.05%) than for contaminated wound surgeries (mean, 88.30%). A high percentage was achieved by the 3 groups. The rate of skin antiseptic preparation compliance was higher in the clean wound group (mean, 89.05%) and the clean-contaminated wound group (mean, 90.70%) than the contaminated wound group (mean, 68.12%). The lower rate might be due to time constraints in contaminated wound operations. Only 0.18% of clean-wound operations had SSIs, and the clean-contaminated wound group had 0.19% SSIs, whereas no SSIs occurred in the contaminated and dirty wound groups. The overall SSI rate was 0.17%; thus, we achieved our goal. Conclusions: A bundle of preoperative infection-prevention preparations reduced the rate of SSI. Furthermore, the bundle had a highly tangible positive impact for both internal and external stakeholders, and it was effective in ensuring good practice regarding preoperative preparation.

Utilization of Secang Wood Ethanol Extract (Caesalpinia sappan L.) as an Additional Ingredient Antiseptic Hand Cream Preparations Instead of Hand Sanitizer

Secang wood is a plant used in medicine. This study aims to find out that ethanol extract of secang wood can be formulated into a hand cream preparation and to determine the antibacterial activity against Staphylococcus aureus as an antiseptic. This study used the maceration method to produce ethanol extract, then made a hand cream dosage formula using 2, 4, and 6% ethanol extract of secang wood. Then a physical quality evaluation is carried out including organoleptic testing, homogeneity testing, pH measurement, viscosity testing, and dispersion power. After that, an antiseptic test of Staphylococcus aureus is carried out. Based on research, the results show that hand cream preparations meet the standards. The anti-bacterial activity test of the hand cream extract of wood ethanol extract can inhibit the growth of Staphylococcus aureus with a concentration of 2, 4, and 6% respectively by 13.1; 13.9 and 14.8 mm fall into the category of inhibitory solid zones.

Irritant contact dermatitis due to povidone-iodine following a surgical intervention: An unusual case report

The most common day-to-day antiseptic preparation used before any surgery usually involves povidone-iodine. Any irritant reaction to it can be devastating for the patient’s aspect and would raise the need for a preliminary investigation prior to going through any antiseptic preparation. In literature, very few cases were reported on irritant dermatitis with povidone-iodine in Indian setting. We present a case of an 18-year-old female presented with irritant contact dermatitis due to povidone-iodine following a surgical intervention.

Antimicrobial activity of aqueous dispersions of silver nanoparticles against pathogens of purulent-inflammatory diseases

Currently, metal nanostructures are widely used in medical, microbiological, and veterinary practice. Silver nanoparticles are especially promising as antimicrobial agents, becauseno published data regarding antimicrobial resistance are available. Whiledeveloping preparations based on metal nanoparticles, an important remainingissue is the choice of a stabilizer, introduction of which during the synthesis ensures the preservation of structures at the nanoscale range, and, consequently, relevant main characteristics, including biocidal properties. The object of the study was to investigate silver nanoparticle aqueous dispersions stabilized by natural and synthetic polymeric compounds. Routine strains of Gram-positive and Gram-negative bacteria were used as experimental models: S. aureus 209 P, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 3177 (O-form), Klebsiella pneumoniae ATCC 31488, obtained from the Scientific Centre for Expert Evaluation of Medicinal Products. The antimicrobial activity of diverse variants of silver nanoparticle aqueous dispersions was assessed by serial dilution platingon dense nutrient medium. In this work, we examined no effect of silver nanoparticles without stabilizers, because their absence led to rapid agglomeration of nanostructures and loss of nanoscale characteristics. The highest sensitivity of Gram-positive and Gram-negative bacteria was foundto the action of ansilver nanoparticle aqueous dispersions stabilized by polyazolidinammoniumand modified with iodine hydrate ions. Drug working concentrations ranging from 0.5 to 3% had a bactericidal effect against pathogens of purulent-inflammatory diseases, and the minimum working concentration of 0.125% led to decreased colony-forming units by 2057% for diverse bacterial strains. Silver nanoparticles stabilized with sodium dodecyl sulfate showed high efficiency against the studied test strainsprobably due to the high toxicity of the stabilizer used as was previously established during a comprehensive safety assessment using biotest objects and cell cultures. In this regard, its use as a component of antimicrobial preparations is not preferred. The results of the studies showed that among the variants of silver nanoparticle aqueous dispersions, preparations stabilized with polyvinyl alcohol and polyazolidinammonium modified with iodine hydrate ions are the most promising for use in biomedical practice, because they demonstrate a high level of antibacterial activity against both Gram-positive and Gram-negative bacteria as causative agents of purulent-inflammatory diseases and a low toxicity level. This allows us to recommend them as safe and effective antimicrobial components indisinfectants, as well as antiseptic preparations for prevention and treatment of skin and soft tissue infectious diseases.

Green Synthesis of Silver Nanoparticles Using Bellevalia Flexuosa Leaves Extract

Silver nanoparticles (AgNPs) have broad biocidal activities, and are widely employed as an active ingredient in antiseptic, anti-viral, and anti-inflammatory preparations. Green-synthesizing AgNPs would be a rapid, cheap, and environmentally friendly method of synthesis. The methanolic extract of the leaves of Bellevalia flexuosa Boiss. (Asparagaceae) was used for the green synthesis of the AgNPs. The effects of the pH and the concentration of silver nitrate (AgNO3) on the synthesis of the AgNPs were investigated. The AgNPs produced above pH 10, and 1 mM of AgNO3 resulted in lower hydrodynamic diameters. Ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction proved the formation of the AgNPs, with a face-centered, cubed geometry. Scanning electron microscopy images showed colloidal and well-dispersed nanoparticles. In addition, the antibacterial activities of the prepared AgNPs were assessed by optical densities (ODs) against Gram-positive bacteria (Enterococcus faecalis and Staphylococcus epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Salmonella enterica). The broths of Gram-negative and Gram-positive bacteria that contained AgNPs, showed lower OD values compared to the controls. In conclusion, AgNPs were prepared using B. flexuosa methanolic extract, and showed antibacterial activity against the tested bacterial strains.

A Randomized Clinical Trial Comparing the Efficacy and Safety of 2% Versus 4% Chlorohexidine Gluconate for Surgical Preparation of the Vagina

(Abstracted from Female Pelvic Med Reconstr Surg 2022;28:400–407) Surgical site infection monitoring has the goal of reducing postoperative complications and is a common metric for evaluating hospital safety. Because proper antiseptic preparation at a surgical site can significantly reduce infections, the Centers for Disease Control and Prevention recommends after an alcohol-based prep of skin incisions with antisepsis.

Selective biomarkers for inflammation and infection are associated with post-operative complications following transperineal template prostate biopsy (TTPB): a single-centre observational clinical pilot-study

BackgroundProstate cancer (PCa) and benign prostatic hyperplasia (BPH) are the most common prostate disorders in the UK, which cause considerable ill health in older men. Transperineal template prostate biopsy (TTPB) has emerged as a reliable procedure for the histopathological diagnosis of PCa and BPH due to its higher cancer detection rates. Although antiseptic preparation and antibiotic prophylaxis are used to ensure safety in patients undergoing surgical intervention, post-operative complications, such as infection and bleeding are still unavoidable, resulting in re-admissions, with resource implications. Currently, there is no biomarker profile to predict outcomes or monitor patients during the post-operative course. The main aim of this single-centre observational clinical pilot-study was to investigate the role of inflammatory and infection biomarkers following TTPB and their association with post-operative complications.MethodsForty-five patients scheduled for elective TTPB were recruited after informed consent at the Wrexham Maelor and Glan Clwyd Hospitals, North Wales, UK (n = 45). Prior to surgery, venous blood samples were collected at baseline and subsequently at 30, 120, and 240 min post-operatively. Urine samples were collected before and 120 min after the procedure. Serum procalcitonin (PCT), serum ferritin, and urine B2MG analysis were done using enzyme-linked fluorescent assay (ELFA) and the magnetic Luminex® multiplex performance assay was used to analyse IL-6, IL-8, IL-10 and TNF-α plasma concentrations. Data on clinical outcomes were collected from patients’ medical records.ResultsFollowing TTPB, significant (p ≤ 0.05) increases were observed in uB2MG, IL-6, IL-8, IL-10 and TNF-α. Significant decreases were observed in ferritin (p ≤ 0.05). No significant change was observed in PCT concentration (p ≥ 0.05). One patient developed an infection and severe haematuria post-operatively following TTPB.ConclusionAlthough not confirmative, changes seen in biomarkers such as uB2MG, IL-10 and TNF-α in our observational clinical pilot-study may warrant further investigation, involving larger cohorts, to fully understand the role of these biomarkers and their potential association with post-operative complications such as infection and bleeding which can develop following TTPB for the diagnosis of PCa and BPH.

Antimicrobial persistence of two alcoholic preoperative skin preparation solutions

Normal skin flora and suboptimal skin antisepsis are the primary drivers of healthcare-associated infections (HAIs). Antimicrobial persistence of preoperative skin preparation is necessary to limit microorganisms on the skin and help minimize their entry into an incision or device-insertion site after application. To assess the antimicrobial persistence of two preoperative skin preparation solutions. A randomized, single-centre, partially blinded, clinical study was conducted in 103 healthy volunteers to evaluate the persistent antimicrobial properties of BD ChloraPrep™ (2% w/v chlorhexidine gluconate [CHG]+ 70% v/v isopropyl alcohol [IPA]) and BD PurPrep™ (8.3% w/w povidone-iodine [PVPI]+ 72.5% w/w IPA) skin preparations out to 7 days and 96h, respectively, on abdomen and groin testing sites. An additional 32 healthy volunteers participated in a neutralization procedure to ensure that the study recovery solution was non-toxic to microorganisms, and a spore-recovery procedure to demonstrate that microorganisms could be successfully recovered from the PVP-I+IPA film-forming product. Both CHG+IPA and PVP-I+IPA produced a mean bacterial log10 reduction >2 and >3 on the abdomen and groin, respectively, 10min after application. CHG+IPA maintained antimicrobial persistence out to 7 days post application, whereas PVP-I+IPA maintained antimicrobial persistence out to 96h post application, the longest time-point selected for this product. CHG+IPA and PVP-I+IPA were both found to be effective, persistent antiseptic skin preparations. Overall, skin irritation was uncommon, and only one adverse event occurred following product application, which was not considered product-related but was considered procedure-related.

Formulasi dan uji aktivitas antibakteri sediaan gel antiseptik tangan ekstrak daun puring (Codiaeum variegatum)

The high incidence of infection occurs due to a lack of public awareness in getting used to a clean and healthy life. Hands are one of the media for the spread of bacteria, so an antibacterial preparation is needed. Puring leaf (Codiaeum variegatum) is a plant that contains many components of secondary metabolites, one of which is tannin compounds. Based on previous research, it has been proven that the tannin group has antibacterial activity. The use of puring leaves directly on the hands is considered less practical so it needs to be formulated in the form of hand antiseptic gel preparations. This study aims to design a hand antiseptic gel formula with variations in the concentration of puring leaf extract and to determine the antibacterial activity of hand antiseptic gel preparations from puring leaf extract against Staphylococcus aureus bacteria. This type of research was experimental laboratory. Puring leaves were first extracted using 96% ethanol as solvent and three hand antiseptic gel formulations were made with varying concentrations of extract, namely FI (1.5%), FII (4.5%), and FIII (6%) with a base gel using carbopol 940. The physical tests included organoleptic and homogeneity tests, pH tests, dispersion tests, and viscosity tests. The data obtained were analyzed using ANOVA and Kruskal Wallis. The antibacterial activity test was measured using the paper disk diffusion method. The results showed that the three formulations of hand antiseptic gel preparations puring leaf extract met the physical characteristics of good gel preparations and FIII had the highest antibacterial activity against Staphylococcus aureus with an inhibitory diameter of 3.16±0.28. Antibacterial activity in hand antiseptic gel preparation of puring leaf extract is in the weak category. Keywords: Antibacterial; puring leaf; paper disk difusi; antiseptic gel

THE ANTIBACTERIAL ACTIVITY OF BETEL (Piper battle L.) AND BASIL (Ocimum sanctum L.) LEAVES INFUSION AS ANTISEPTIC PREPARATIONS AGAINST SOME BACTERIA IN VITRO

Betel leaves (Piper battle L.) and basil leaves (Ocimum sanctum L.) are parts of the plant that can be made as antiseptic preparations because they contain antibacterial compounds such as flavonoids, tannins, and saponins. Antiseptic activity can be observed based on its effect in inhibiting the colonization of standard test bacteria of the laboratory, namely Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi as well as the magnitude of the phenol coefficient value. The study aimed to analyze the infusion activity of betel and basil leaves as antiseptic preparations against some test bacteria. This study in vitro used a posttest-only control group design. The infusion treatment of betel and basil leaves is tested in the form of a single preparation and a combination with concentrations of 50%, 75%, and 100% (w/v). The results showed there was a difference in the infusion activity of betel leaves and basil leaves in a single stock and a combination of the number of bacterial colonies tested; the effect of the treatment produces a small number of bacterial colonies in Gram-positive bacteria; 100% combination treatment (b/v) provides an inhibitory effect that is not significantly different from 70% alcohol control against all test bacteria except S.typhi: and obtained the phenol coefficient value from the combination treatment of betel and basil leaves infusion higher against Gram-positive bacteria. The conclusion of this study, the combination of betel and basil leaves infusion has antibacterial activity as an antiseptic preparation that can inhibit the growth of some test bacteria.
 
 Keywords: antiseptic preparations, infusion leaves, Piper battle L, Ocimum sanctum L, number of colonies, phenol coefficient

Description of non-brachycephalic canine conjunctival microbiome before and after application of an antiseptic preparation.

To evaluate canine conjunctival microbiome before and after an antiseptic preparation using aerobic culture and DNA sequencing. Six healthy non-brachycephalic dogs. Dogs randomly received povidone-iodine 1:50 dilution solution in one eye with the second eye serving as a control. Standardized volumes of solution, number of sterile cotton tipped applicators, and preparation times of 5 min were used. Aerobic culture and DNA sequencing samples were collected from inferior conjunctival fornices at baseline, immediately following, 24 h, and 4 weeks following antiseptic preparation. Aerobic culture data were evaluated using paired t-test and linear regression. Illumina platform was used to sequence bacterial DNA using primers to target the V4 region of bacterial 16S rRNA. Quantitative Insights Into Molecular Ecology (QIIME 2.0) was used to analyze data. Baseline aerobic cultures consisted of Bacillus (35%), Staphylococcus (30%), Streptococcus (20%), Moraxella (5%), Micrococcus (5%), and Simonsiella spp. (5%). No significant difference was detected in mean number of species cultured at baseline and following treatment (p=.465). Treatment, sample time, or interaction was not significant (p > .393). The most abundant phyla at baseline via DNA sequencing were Proteobacteria (57.04%), Actinobacteria (19.89%), Firmicutes (3.25%), and Bacteroidetes (5.5%). Alpha and beta diversity matrices at baseline and over time revealed no significant change in species richness or bacterial composition. Relative abundance of bacterial taxa did not significantly differ in treated or control eyes over time. Conjunctival bacterial flora identified differed between culture dependent and independent methods. The bacterial community remained stable over time after application of the antiseptic preparation.

PD41-03 ANTIBIOTIC-FREE TRANSPERINEAL PROSTATE BIOPSY: UROLOGY’S CONTRIBUTION TO RESPONSIBLE ANTIMICROBIAL STEWARDSHIP

PD41-03 ANTIBIOTIC-FREE TRANSPERINEAL PROSTATE BIOPSY: UROLOGY’S CONTRIBUTION TO RESPONSIBLE ANTIMICROBIAL STEWARDSHIP

Formulasi dan Evaluasi Sediaan Sabun Padat Antiseptik Ekstrak Etanol Daun Kunyit

The plant of findings turmeric (Curcuma longa) are often used as a traditional medicinal. The part of the turmeric plant that is often used is the rhizome. In addition to turmeric rhizome, turmeric leaves are also used as a flavoring dish. Among the benefits of turmeric leaves is as an antimicrobial. Soap is a surfactant used for washing and cleaning. Antiseptics are inhibitors for the growth and development of microorganisms that live on the surface of the body. The study was to determine which ethanol extract of turmeric leaves could be formulated into antiseptic solid soap preparations. This study used an experimental method, turmeric leaves were extracted with 70% ethanol. The formula made are 2.5%, 5%, 7.5%. The quality of the soap preparation was carried out by organoleptic tests, acidity, high foam and irritation. The results test showed that the solid soap did not have the aroma of turmeric leaves, the pH of the soap was 9.9-10.5; foam height with a concentration of 7.5% which is 7.3 cm; and solid soap preparations ethanol extract of turmeric leaves did not cause irritation. The extract of turmeric leaves can be formulated into solid soap preparations. It is recommended to add perfume to the formulation of solid soap preparations of turmeric leaf extract as an antiseptic.

Microbiological Evaluation of Novel Bis-Quaternary Ammonium Compounds: Clinical Strains, Biofilms, and Resistance Study.

This work is devoted to the investigation of biocidal properties of quaternary ammonium compounds (QACs) based on pyridine structures with aromatic spacers, and their widely known analogs, against clinically significant microorganisms. This study is focused on investigating their antimicrobial activity (minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs)), antibiofilm properties (minimum biofilm inhibitory concentrations (MBICs) and minimum biofilm eradication concentrations (MBECs)), synergetic effect with different alcohols in antiseptic formulations, and bacterial resistance development. It was shown that all combined analogue preparations had a higher level of antibacterial activity against the tested bacterial strains, with a 16- to 32-fold reduction in MICs and MBCs compared to previously used antiseptic preparations. Moreover, hit-QACs demonstrated a stable effect against Gram-negative E. coli, K. pneumoniae, and A. baumannii within a month of incubation. Overall results indicated a high level of antibacterial activity of pyridine-based QACs.

The effectiveness of prophylactic antibiotics and betadine skin preparation on cranial cutaneous Cutibacterium acnes – A prospective study

BackgroundCutibacterium acnes, formerly known as Propionibacterium acnes, is increasingly recognized as a cause of surgical site infection and implant failure despite the use of prophylactic antibiotics and antiseptic surgical preparations. The aim of this study was to investigate whether C. acnes persists in the dermal layer of the skin after standard perioperative antibiotics and skin prepping with alcoholic betadine solution in consecutive patients undergoing a craniotomy. MethodsA single centre prospective observational study was performed at Flinders Medical Centre. Adult patients undergoing a cranial neurosurgical intervention between October 2019 to March 2021 were eligible for inclusion. After administration of standard preoperative antibiotics (Cefazolin), three swabs were taken for each patient: one before prepping the skin with alcoholic betadine, one after prepping the skin and a dermal swab once the skin was incised. Results73 patients were included. Cutibacterium acnes cultures were positive in 61 patients of the “pre-prep” group (83.6%), 12 (16.4%) in the “post-prep” group, and 53 (72.6%) were from dermal swabs There was a significant reduction of positive cultures of the skin after surgical preparation was applied (p < 0.00001). There was a non-significant reduction of positive cultures in the dermal swabs after skin preparation (p = 0.068) ConclusionsCutibacterium acnes persists within the dermis of the scalp despite standard prophylactic measures using alcoholic betadine solution and cefazolin.

The potential of citronella grass to inhibit growth of Escherichia coli and Staphylococcus aureus bacteria

Background: Citronella grass (Cymbopogon nardus (L.) Rendle) has a distinctive aroma that comes from its essential oils. Essential oil components, especially citronella and geraniol compounds, are known to have antifungal and antibacterial activities. Therefore, citronella grass extract can be used as an active ingredient for antiseptic hand gel products. However, it is necessary to optimise the gelling agent to get the optimal formulation. Aim: This study aimed to determine the concentration mixture from three gelling agents that were optimal, stable, and able to inhibit bacterial growth: Sodium carboxymethyl cellulose (CMC-Na), gum arabic, and gelatin. Method: Citronella grass was macerated using 70% ethanol. Gelling agents’ variation were evaluated based on the viscosity, spreadability, and adhesion. The most optional variation was obtained using analysis with the Simplex Lattice Design method. The antimicrobial activity of the extracts and the gel were tested using the disc diffusion method against Escherichia coli and Staphylococcus aureus. Result: The results showed that the optimal gelling agents for the specified parameters was a mixture of CMC-Na and gelatin (1:1). This formulation can inhibit the growth of bacteria. Conclusion: Several different types of gelling agents’ mixture for hand antiseptic gel preparation were made from the results of this study. It is necessary to develop a gel that can increase the antimicrobial activity of the active substance of citronella grass extract.

Synthesis of Silver Nanoparticles Balaccida Leaf Extract and Its Anti-Bacterial Activity of E. Coli

The Covid-19 pandemic has become a major problem for public health around the world. One of the ways to prevent Coronavirus is by maintaining hand hygiene using hand sanitizer. The main ingredient of most antiseptics circulating in Indonesia is alcohol which can cause skin irritation. Alcohol substitutes as antiseptics in hand sanitizers are widely available in nature, one of which is the balakacida plant (Chromolaena odorata). The availability of balakacida plants in Indonesia is very abundant as wild plants are often referred to as nuisance plants (weeds). The phytochemical content of balakacida leaves includes saponins, flavonoids, tannins, and alkaloids that have the potential as antibacterial. Balakacida leaf extract has the potential to be used as a source of active ingredients in antiseptic preparations as an alternative to maintain hand hygiene from various parasitic microorganisms. AgNPs are one of the antibacterial and antifungal agents that are effective in inhibiting and killing microorganisms. The optimal variation of CO-SNPs was found at 3 ml of CO-SNPs with an absorption peak of 409 nm and a particle size of 230.1 nm. CO-SNPs produced the best inhibitory value of 12.26 mm on E.coli bacteria.