In this paper, the physicochemical properties, antioxidant and anti-aging abilities of three new Tremella hydrocolloids were studied. The physicochemical properties were characterized by Fourier transform infrared spectroscopy, differential scanning calorimeter, X-ray diffractometry etc. The antioxidant activities of Tremella hydrocolloids were determined by ABTS radical, DPPH radical scavenging activity. The anti-aging ability of Tremella hydrocolloids was also investigated by using the organism model of Caenorhabditis elegans (C. elegans). The results showed that the ES-THD displayed the highest radical scavenging capacity and the best anti-aging abilities. The ability of ES-THD to scavenge ABTS radicals reached 100 % at 2 mg/mL, the ability of ES-THD to scavenge DPPH radicals reached 45.55 %. Compared with the control group, the average lifespan was 29.17 % longer fed with ES-THD. These results provide the evidence basis for the use of Tremella hydrocolloids as food texture modifiers, antioxidants, and anti-aging agents in the food industries.