Antioxidant Status In Saliva Research Articles

Indicators of chemiluminescent and bioluminescent tests of biological liquids in the assessment of physical health.

The study includes anthropometry of 172 young male, obtained data on the length and body mass, measured the transverse diameters of the shoulders and pelvis, various body types was identified by the J.M. Tanner sexual dimorphism index (andromorphic, mesomorphic, gynecomorphic). The chemiluminescent and bioluminescent study of saliva and blood was conducted in the examined young male. We studied the indicators of the antioxidant defense system under the influence of stress. The antioxidant status of saliva was determined using the H2O2-luminol-dependent chemiluminescence method. Data on the activity of NAD (P) -dependent dehydrogenases in blood lymphocytes was obtained from a bioluminescent method of research. Young male of andromorphic body type had large overall and transverse body sizes. Indicators of antioxidant protection of saliva and blood in men of adolescence, the body type of the sexual dimorphism index J.M. Tanner was different. The persons of the andromorphic body type differed in terms of chemiluminescence in comparison with the young male of gynecomorphic body type. The results of bioluminescent blood tests suggest a violation of the catabolic and anabolic processes of carbohydrate and fat metabolism in young men of mesomorphic and gynecomorphic body types. Indicators of the system of antioxidant protection of saliva and blood reflect the sexual characteristics of the body of young male and can be used as additional criteria for diagnosing sex inversion and assessing the risk of developing socially attributed diseases.

ANTIOXIDANT STATUS AS THE MARKER OF THE HEALTH IN STUDENTS IN THE TIME OF THEIR INTENSIVE INTELLECTUAL ACTIVITIES

Currently, the implementation of innovative non-invasive methods for controlling the state of the human organism under stress conditions is highly actual. Long-time intellectual burdens lead to the chronic stress and may result in the exhaustion of antioxidant system. The aim of the research is to study the characteristics of the antioxidant status in saliva under the conditions of intensive intellectual activities. In order to carry out the experiment, we collected the cohort of 123students aged from 20 to 22 years of the second and third years of the V.F.Voino-Yasenetsky Krasnoyarsk State Medical University. The examined materials, namely saliva, had been taken by direct spitting into test-tubes. Saliva was collected twice: firstly background sampling had been taken during the course of regular classes when the students were supposed to be in the state of emotional balance; secondly, the test sampling had been made in the time of examination session characterized by considerable intellectual burdens. We carried out questionnaire survey for the students using Spielberger-Hanin and Nemchin-Taylor tests. With the help of these tests, we determined the situational and personal anxiety as well as the predisposition to stress development. Antioxidant status was evaluated by Н2О2-luminol-dependent chemiluminescence technique. Chemiluminescent tests were performed by tablet luminometer TriStar LB 941, (made by Berthold). We had proved the existence of the association between the antioxidant status of saliva and the state of intellectual tension. We have noted that during a session in a group with the high level of uneasiness indices have grown, in a group with the low level of uneasiness they have decreased. We can suppose that in the time of examination sessions there is an increase in the common level of anxiety under the lowering of antioxidant activity. In intellectual stress, the antioxidant system performs the interception of radicals less intensively, because the speed of active forms of oxygen (AFO) neutralization is decreased. So, under the conditions of intensive intellectual activities, the degradation of antioxidant protection is observed. It is caused, supposedly, by the lowering of the activity of the enzymes of peroxidase protection. The pro-oxidant system also works less efficiently. This is confirmed by the decline of such markers of CL-fluorescence as maximum intensity, amplitude and light sum, which showed AFO number.

Estimation of Antioxidant Levels in Saliva and Serum of Chronic Periodontitis Patients with and without Ischemic Heart Disease.

Objective To investigate whether there is a relationship between periodontitis and ischemic heart disease by estimation of total antioxidant status in saliva and serum. Materials and Methods A total of 80 samples were collected and divided equally into 4 groups of healthy controls, chronic periodontitis patients, ischemic heart disease patients with periodontitis, and ischemic heart disease patients without periodontitis. Saliva and venous blood samples were collected and analyzed for levels of total antioxidant capacity, superoxide dismutase, glutathione peroxidase, and catalase. Results There were significant (p < 0.05) differences in the mean serum levels of total antioxidant capacity (p < 0.001), superoxide dismutase (p < 0.001), glutathione peroxidase (p < 0.006), and catalase (p < 0.001) within the 4 groups, whereas the mean salivary levels were significant only for glutathione peroxidase (p = 0.001). Both of these serum and salivary antioxidant levels were lower in disease groups of IHD + CP, IHD + H, and CP as compared to healthy controls, with different patterns. Conclusion Antioxidant capacity is significantly hampered in chronic periodontitis and ischemic heart disease patients with or without periodontitis as compared to healthy controls. The salivary and serum antioxidants may not follow the same increase or decrease as a result of increased oxidant stress due to disease.

Nonenzymatic antioxidants in saliva of patients with systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoantibody-directed self-antigens, immune complex formation and immune deregulation, resulting in damage to essentially all the organs. SLE is associated with the increased production of free radicals. Increase in free radicals or impaired antioxidant defense system in SLE causes oxidative stress. Considering that saliva could be a reflection of the state of health, the purpose of this study was to evaluate some antioxidants in the saliva and serum of patients with SLE and compare these with healthy individuals. This could help us in obtaining a possible marker in saliva in the future. During the course of the practical part of the project, 30 patients with SLE and 30 healthy controls were investigated. After centrifugation of un-stimulated saliva and blood samples, they were examined using spectrophotometric methods and the results were analyzed by statistical software. According to the results, concentrations of malondialdehyde, uric acid and total antioxidants were significantly increased but the level of reduced glutathion was reduced significantly in the saliva and serum of SLE patients as compared to controls. It is therefore suggested that antioxidant power is impaired in saliva and serum of SLE patients. As there was a positive correlation between the antioxidant level of saliva and blood serum, the antioxidant status of saliva could be an indicator of serum antioxidants.

Alternations of salivary antioxidant enzymes in systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is an autoimmune disease with chronic systemic inflammation. Oxidative stress may play a role in the pathogenesis of SLE. An increase in free radicals or an impaired antioxidant defense system in SLE causes oxidative stress. Therefore, oxidative damage plays an important role in the pathogenesis of SLE. Variations in antioxidant activity have been previously studied in serum of patients with this disease. However, salivary factors have not been evaluated. Considering that saliva, the noninvasive biological fluid, could be a reflection of the state of health, the purpose of this study was evaluation of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) activity in the saliva of patients with SLE. During the course of the practical part of the project, 30 patients with SLE and 30 healthy controls were selected to donate their saliva samples. After centrifugation of un-stimulated saliva, biological activity of POD, CAT and SOD were evaluated on their appropriate substrates using spectrophotometric methods and the results were statistically analyzed. The results showed that activities of antioxidant enzymes SOD and CAT were significantly reduced in saliva of SLE patients as compared to controls. The results suggest that antioxidant status was impaired in the saliva of SLE patients, and antioxidant status of saliva could be one of the non-invasive markers for SLE.

Tai Chi exercise increases SOD activity and total antioxidant status in saliva and is linked to an improvement of periodontal disease in the elderly.

The aim of this study was to determine the effect of Tai Chi on biological markers of oxidative stress in saliva and its relationship with periodontal disease (PD) in older adults. We carried out a quasi-experimental study with a sample of 71 sedentary volunteers with PD who were divided into a control group of 34 subjects and an experimental group of 37 subjects who performed Tai Chi 5 days a week for a period of 6 months. PD status was characterized using the Periodontal Disease Index (PDI). Superoxide dismutase (SOD), total antioxidant status (TAS), and TBARS levels of both groups were measured by spectrophotometric methods. In addition, inflammation markers (TNF-α, IL-1β, IL-6, IL-8, and IL-10) were measured by flow cytometry. We found a statistically significant increase in SOD activity (P < 0.001) and TAS concentration (P < 0.05), whereas levels of IL-1β were significantly lower (P < 0.01). Likewise, a statistically significant decrease in the PDI (P < 0.05) was observed in subjects who performed Tai Chi during a period of 6 months. Our findings suggest that the practice of Tai Chi has both antioxidant and anti-inflammatory effects that are linked to the improvement of PD in older adults.

Salivary Markers of Oxidative Stress and Their Relation to Periodontal and Dental Status in Children

Background: Previous studies have shown that salivary thiobarbituric acid reactive substances are related to the periodontal status in adults. Such an analysis has not been done on children yet. The aim of our study was to analyze salivary markers of oxidative stress in relation to periodontal and dental status in children.Methods: The periodontal and dental status of 82 consecutive pediatric dental patients was assessed. The oral hygiene index (OHI), the papillary bleeding index (PBI) and the caries index (CI) were assessed as clinical parameters. Markers of oxidative stress and antioxidant status were measured in whole saliva samples.Results: Multivariate analysis of covariance showed that the variability of PBI explains 10.9% of the variance of salivary thiobarbituric acid reacting substances (TBARS). Advanced oxidation protein products (AOPP) were related to CI (eta 8.6%). Measures of antioxidant status (total antioxidant capacity and ferric reducing ability of saliva) were partially determined by OHI (13.6% and 7.2%) and PBI (16.9% and 7.9%).Conclusions: Antioxidant status in saliva is related to oral hygiene and periodontal status. Salivary TBARS are a potential sensitive marker of periodontitis in children, similarly to adults, at least on a population level. Salivary AOPP are related to caries. Potential diagnostic value of the analyzed markers should be analyzed in further interventional studies.

Salivary markers of oxidative stress and their relation to periodontal and dental status in children.

Background: Previous studies have shown that salivary thiobarbituric acid reactive substances are related to the periodontal status in adults. Such an analysis has not been done on children yet. The aim of our study was to analyze salivary markers of oxidative stress in relation to periodontal and dental status in children. Methods: The periodontal and dental status of 82 consecutive pediatric dental patients was assessed. The oral hygiene index (OHI), the papillary bleeding index (PBI) and the caries index (CI) were assessed as clinical parameters. Markers of oxidative stress and antioxidant status were measured in whole saliva samples. Results: Multivariate analysis of covariance showed that the variability of PBI explains 10.9% of the variance of salivary thiobarbituric acid reacting substances (TBARS). Advanced oxidation protein products (AOPP) were related to CI (eta 8.6%). Measures of antioxidant status (total antioxidant capacity and ferric reducing ability of saliva) were partially determined by OHI (13.6% and 7.2%) and PBI (16.9% and 7.9%). Conclusions: Antioxidant status in saliva is related to oral hygiene and periodontal status. Salivary TBARS are a potential sensitive marker of periodontitis in children, similarly to adults, at least on a population level. Salivary AOPP are related to caries. Potential diagnostic value of the analyzed markers should be analyzed in further interventional studies.

Comparison of antioxidant enzymes activity and the concentration of uric acid in the saliva of patients with oral cavity cancer, odontogenic cysts and healthy subjects

Chronic inflammation is related to oxidative stress and is still believed to be the cause of carcinogenesis. Patients with oral cavity cancer (OCC) exhibited lower total antioxidant capacity, uric acid (UA) concentration, salivary peroxidise (SPO) and superoxide dismutase (SOD) activity in their saliva than did healthy subjects. This could be a risk factor for tumour induction. Odontogenic cysts also arise in response to locally acting proinflammatory factors, for example, a gangrenous tooth. Furthermore, cyst development is accompanied by chronic inflammation. There are some reports in the literature concerning primary tumours such as squamous cell carcinomas arising from odontogenic cysts. The reason for this transformation is still unknown. The aim of this study was to compare the status of the antioxidant defence system in the saliva of the group with odontogenic cysts and OCC with that of the healthy control. Saliva samples were collected in the morning. SOD, SPO activity and UA concentration were determined using standard methods. Patients with odontogenic cysts and OCC exhibited lower activity of major antioxidants in their saliva (SPO, UA) than did healthy people. SOD activity and age are the main factors that distinguish these diseases. Discriminant function analysis showed that once data such as antioxidant status of saliva, age and smoking status are known 80% cases can be correctly classified as healthy, 80% as having odontogenic cysts and 40% as cancerous. To conclude, the decrease in concentrations of major antioxidants in the saliva of patients with cysts may increase the risk of neoplastic transformation especially in advanced age.

The antioxidant status of saliva and blood plasma in depression : A.I. Lukash, V.G. Zaika, N.P. Milutina, A.O. Kucherenko. The Department of Biochemistry, Rostov State University, B. Sadovay, 105, 344006. The Department of Psychiatry, Rostov State Medical University, Nakhichevansci, 31, 344022, Rostov-on-Don, Russia

The antioxidant status of saliva and blood plasma in depression : A.I. Lukash, V.G. Zaika, N.P. Milutina, A.O. Kucherenko. The Department of Biochemistry, Rostov State University, B. Sadovay, 105, 344006. The Department of Psychiatry, Rostov State Medical University, Nakhichevansci, 31, 344022, Rostov-on-Don, Russia