Abstract
Background: Previous studies have shown that salivary thiobarbituric acid reactive substances are related to the periodontal status in adults. Such an analysis has not been done on children yet. The aim of our study was to analyze salivary markers of oxidative stress in relation to periodontal and dental status in children.Methods: The periodontal and dental status of 82 consecutive pediatric dental patients was assessed. The oral hygiene index (OHI), the papillary bleeding index (PBI) and the caries index (CI) were assessed as clinical parameters. Markers of oxidative stress and antioxidant status were measured in whole saliva samples.Results: Multivariate analysis of covariance showed that the variability of PBI explains 10.9% of the variance of salivary thiobarbituric acid reacting substances (TBARS). Advanced oxidation protein products (AOPP) were related to CI (eta 8.6%). Measures of antioxidant status (total antioxidant capacity and ferric reducing ability of saliva) were partially determined by OHI (13.6% and 7.2%) and PBI (16.9% and 7.9%).Conclusions: Antioxidant status in saliva is related to oral hygiene and periodontal status. Salivary TBARS are a potential sensitive marker of periodontitis in children, similarly to adults, at least on a population level. Salivary AOPP are related to caries. Potential diagnostic value of the analyzed markers should be analyzed in further interventional studies.
Highlights
Oxidative stress is a dysbalance between the production of free radicals and antioxidant status leading to oxidative damage of macromolecules including lipids and proteins
The oral health status of subjects was assessed using the modified oral hygiene index (OHI: 0 – no plaque present, 1 – plaque covers less than one third of tooth surface, 2 – plaque covers more than one third of tooth surface), papillary bleeding index (PBI: 0 – no bleeding on probing, 1 – subtle bleeding on probing, 2 – moderate to severe bleeding on probing) and caries index (CI: 0 – no caries, 1 – superficial lesion, 2 – lesion affecting the dentin)
The obtained data revealed that the model composed of age, gender and the analyzed clinical parameters describes 35.9% of the variance of thiobarbituric acid reacting substances (TBARS), 13% for Advanced oxidation protein products (AOPP), 8.6% for advanced glycation end products (AGEs), 20% for total antioxidant capacity (TAC) and 14.5% for ferric reducing activity of saliva (FRAS)
Summary
Oxidative stress is a dysbalance between the production of free radicals and antioxidant status leading to oxidative damage of macromolecules including lipids and proteins. The palette of oxidative stress markers is wide, but only few of them were studied in saliva and in relation to the status of oral tissues. Previous studies have shown that salivary thiobarbituric acid reactive substances are related to the periodontal status in adults. Such an analysis has not been done on children yet. The aim of our study was to analyze salivary markers of oxidative stress in relation to periodontal and dental status in children. Results: Multivariate analysis of covariance showed that the variability of PBI explains 10.9% of the variance of salivary thiobarbituric acid reacting substances (TBARS). Salivary TBARS are a potential sensitive marker of periodontitis in children, to adults, at least on a population level. Potential diagnostic value of the analyzed markers should be analyzed in further interventional studies
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