Antioxidant Potential Research Articles

Ultrasonic-assisted DES extraction optimization, characterization, antioxidant and in silico study of polysaccharides from Angelica dahurica Radix

In this study, polysaccharides from Angelica dahurica Radix (ADR) were extracted by using ultrasonic-assisted deep eutectic solvent (DES) extraction method. The DES consist of choline chloride (HBA, hydrogen bonding acceptor) and 1, 4-butanediol (HBD, hydrogen bonding donor) with the molar ratio of 1:3 was proved as the best system for polysaccharides extraction. A Box-Behnken design with three factors and three levels was utilized to optimize ultrasonic power, extraction time and temperature for increased polysaccharides yield. The optimal extraction parameters were determined as follows: DES water content of 20 %, liquid–solid ratio of 10:1 mL/g, ultrasonic time of 21 min, temperature at 60 °C, and ultrasonic power of 435 W. These conditions yielded an extraction rate nearly matching the theoretical predicted value of the response surface model, surpassing the traditional hot water extraction method by 1.33 times and requiring less time. The antioxidant polysaccharides was assessed using DPPH,ABTS and Hydroxyl methods, which indicated that ADRP extracted by ultrasound assisted DES had excellent antioxidant capacity and showed a dose–response relationship with drug concentration. Structural analysis identified ADR polysaccharides as a heteropolysaccharide rich in sugar aldonic acids—namely, fucose, rhamnose, arabinose, galactose and glucose—with molar ratios of 0.47:1.99:17.61:64.76:15.17. The polysaccharides’ number-average molecular weight was determined to be 97.422 kDa, with a weight-average molecular weight of 245.678 kDa. Network pharmacology and molecular docking analysis suggest that the monosaccharides Rha and Ara could potentially interact with the proteins GSR and GSTA1, respectively, indicating that ADRP had good antioxidant potential and could be applied in multiple fields with promising development prospects in the future.

The effects of varying ingredients combination and boiling time on total phenolic content, antioxidant activity, and antimicrobial properties of lemongrass-ginger tea

This study was aimed at exploring the effect of varying lemongrass-ginger combinations, and boiling time on total phenolic contents (TPC), antioxidant activity, and antimicrobial efficacy of lemongrass-ginger tea. Lemongrass-ginger tea was produced by varying the percentage of lemongrass (25 %, 50 %, and 75 %) and boiling times (5, 10, and 15 min). The antioxidant activity of the lemongrass-ginger tea samples was investigated using the DPPH and ABTS assays whereas the TPC was determined using the Folin-Ciocalteau method. The antimicrobial activities were investigated by measuring the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of the tea against selected microorganisms, and its combinatory effects with antimicrobial drugs. The lemongrass-ginger combination and the boiling time significantly affected antioxidant potential, TPC, and antimicrobial activities. TPC measured ranged between 966.7 ± 90.20 to 1761.3 ± 81.70 μgGAE/g whereas DPPH antioxidant activities varied from 43.97 ± 14.99 % to 75.20 ± 8.55 %. The highest values of TPC and DPPH were 1761.3 ± 81.70 μgGAE/g and 75.20 ± 8.55 % and were recorded by 75 % lemongrass-ginger combination boiled for 15 min. Furthermore, differences in lemongrass-ginger combination and boiling times resulted in varying antimicrobial activities against the test microorganisms. The lowest MBC was recorded for 50 % lemongrass boiled for 10 min against C. albicans, 75 % lemongrass boiled for 15 min against K. pneumoniae and S. typhi, and 25 % lemongrass against E. coli. Additionally, varying ingredient proportions and boiling times affected the combinatory effect of the tea with antimicrobial drugs. However, the exact effect depends on the proportion of ingredients used and the boiling times.

Postharvest melatonin application attenuates browning, delays softening, and maintains the antioxidant potential of jackfruit bulbs

Although the impact of postharvest melatonin (MLT) application in extending the storage life of horticultural produce has been widely studied, its effect in jackfruit remains unknown. This investigation evaluates the influence of MLT dip application (0, 0.05, 0.1, and 0.2 mM) on browning, softening and oxidative stress in cold-stored jackfruit bulbs over a period of 20d. All MLT treatments reduced browning index and oxidative stress, while activities of browning-related enzymes were comparatively lower in the 0.1 mM MLT treatment. Treatment with 0.2 mM MLT maintained elevated levels of antioxidants as compared to control. Higher bulb firmness, total pectin and cellulose content with considerably lower activities of cell wall degrading enzymes were observed in all MLT treatments. In conclusion, MLT (0.1 and 0.2 mM) is an effective treatment for mitigating postharvest browning and oxidative stress in addition to delaying fruit softening and maintaining the antioxidant potential of cold-stored jackfruit bulbs.

Synthesis, evaluation of antioxidant and antidiabetic potential of 3-ethoxy carbonyl coumarin-8-propionamides

A series of 3- ethoxycarbonyl coumarin-8-propionamide analogues were designed, synthesised and in vitro antioxidant potential was screened by DPPH and ABTS assays and examined their anti-diabetic activity against α-amylase enzyme. The 3-fluoro-2-trifluoromethyl phenyl substituted coumarin-8-propionamide (8k) showed excellent scavenging potential (IC50 - 43.1 μM) for both DPPH and ABTS free radicals compared with the standards trolox (IC50 - 43.3 μM) and ascorbic acid (IC50 - 42.1 μM). The compound dicyanophenyl substituted propionamide analogue (8m) displayed highest inhibition with IC50 value of 5.1 μM against the α-amylase enzyme which is well comparable with Metformin (IC50 4.6 μM). The docking studies of the compounds with the receptor protein α-amylase (PDB ID 4x9y) shown lower binding energy when compared with Metformin.

Extract from Psidium guineense Sw leaves: An alternative against resistant strains of Staphylococcus aureus

Psidium guineense SW., commonly known as sour guava, is used in traditional medicine to address diverse health issues, such as inflammation, infections, and gastrointestinal disorders. Although previous research has highlighted the antimicrobial properties of the root extract and the antioxidant potential of its fruits, information regarding the activities associated with the leaf extract is lacking. The aim of the study was to use the traditional knowledge of the biological properties of P. guineense and investigate its antimicrobial activity, mechanism of action, synergistic effects, and antivirulence activity against Staphylococcus aureus. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined for S. aureus, including antibiotic-resistant isolates. The synergy between the extract and antibiotics was evaluated. A growth curve was constructed for S. aureus treated with the extract. The anti-hemolytic activity was assessed by inhibiting hemolysis in human blood, and the inhibition of staphyloxanthin (STX) was examined. The extract effectively inhibited all strains with MIC and MBC values ranging from 256 to 512 µg/mL and 512 to 1024 µg/mL, respectively. Antimicrobial synergy experiments demonstrated the enhanced effectiveness of antibiotics, especially ciprofloxacin, when combined with the P. guineense extract, especially with ciprofloxacin, resulting in complete synergy against all S. aureus strains. In growth curve tests, the extract inhibited growth at MIC of 256 µg/mL within 8 h and significantly delayed it at MIC/2 (128 µg/mL) within 12 h. In antivirulence tests, the extract effectively reduced hemolysis and STX production by S. aureus at MIC and MIC/2, indicating its potential as an antimicrobial agent with antivirulence properties. The extract effectively inhibited S. aureus, including drug-resistant strains with low MIC and MBC values, exhibiting strong antimicrobial and synergistic properties with antibiotics. also In addition, the extract accelerated recovery by limiting bacterial growth and exhibiting antivirulence capabilities, reducing the severe symptoms of S. aureus infections.

Experimental and computational investigations of new organotin(IV) complexes derived from hydrazone ligands: Synthesis, structural analysis and biological assessment

In this article, we have presented the synthesis, structural analysis, and biological assessments of twelve new diorganotin(IV) complexes (derived from heterocyclic hydrazone ligands through condensation reaction between 9-formyl-8-hydroxyjulolidine and aromatic hydrazide derivatives) to discover potent biological agents. The synthesized compounds have been thoroughly characterized using numerous spectroscopic and physicochemical methods, including (1H, 13C and 119Sn) NMR, FT-IR, mass spectrometry, powder XRD and SEM. These scrutinizes indicate that complexes exhibited a pentacoordinated geometry, coordinating through azomethine nitrogen atom, enolic oxygen atom, phenolic oxygen atom and carbon atoms of alkyl/aryl groups of organotin derivatives. DFT (Density functional theory) studies were conducted at the B3LYP/LanL2DZ/6-311G level of hypothesis to investigate the quantum chemical aspects of specified compounds. These properties were facilitated through analyses of charge distribution and molecular orbitals. The antioxidant effectiveness of the prepared compounds was examined employing the DPPH assay and the results revealed that complex 7 exhibited strong antioxidant potential, having an IC50 value of 2.3 µM. Moreover, the prepared compounds were assessed for their effectiveness against four bacterial (B. cereus, B. subtilis, P. aeruginosa, E. coli) as well as two fungal strains (C. albicans and A. niger) using serial dilution approach. Complexes 7, 11, 14 and 15 having MIC values ranging from 0.0051-0.0048 µmol/mL have highest antimicrobial potency equivalent to reference drugs, Fluconazole (MIC = 0.0051 µmol/mL) and Ciprofloxacin (MIC = 0.0048 µmol/mL).

Isolation, Purification, and Antioxidant Activity of Polyphenols from Cynanchum auriculatum Royle ex Wight

Cynanchum auriculatum Royle ex Wight (CA) is a traditional medicinal and edible plant in China. This study aimed to isolate and characterize the phenolic compounds of C. auriculatum to identify its main antioxidant constituents. Polyphenols were extracted using an ultrasound-assisted ethanol extraction method, followed by partitioning with ethyl acetate. The ethyl acetate extract was then purified through thin-layer chromatography, silica gel column chromatography, and reverse-phase silica gel column chromatography. Three monomeric compounds—cynandione A (I), 2,5-dihydroxyacetophenone (II), and radix piperacanthone (III)—were identified through their physical and chemical properties, UV and IR spectra, and liquid chromatography–mass spectrometry (LC-MS/MS). Vitamin C (VC) and 2,4-dihydroxyacetophenone were used as controls to evaluate the antioxidant potential of the two most abundant monomers. Antioxidant assays demonstrated that 2,5-dihydroxyacetophenone and cynandione A exhibited strong antioxidant activity at lower concentrations, whereas 2,4-dihydroxyacetophenone showed significantly weaker activity. Furthermore, cynandione A displayed superior cellular antioxidant activity compared to 2,5-dihydroxyacetophenone, indicating its potential as a promising bioactive compound. In conclusion, this study provides valuable insights into the phenolic composition of C. auriculatum and highlights cynandione A as a key antioxidant, paving the way for future research on its therapeutic applications.

Plantago boissieri : Phytochemical Assessment, Antioxidant, Anti-inflammatoryand Wound Healing Potential.

Plantago is a large genus under family Plantaginaceae. Plantago species were noted for potent antioxidant, anti-inflammatory and wound healing activities. The current research investigated the potential bioactivities as the metabolic content of Plantagoboisserei extract. Results highlighted the rich content of phenolics (450.93 ± 7.4 mg GAE/g extract) and flavonoids (144.2 ± 3.6 mg RE/g extract). HPLC analysis enabled the detection of 17 phenolic constituents among which ellagic acid was found in highest concentration followed by rutin. P. boisserei exhibited a potent antioxidant activity evidenced by the IC50 values in ABTS and H2O2 assays (10.95 and 10.87 µg/mL, respectively) as well as in TAC assay (67.94 mg AAE/g). The anti-microbial activities revealed a moderate activity against Staphylococcus aureus and Proteus vulgaris. In vitro anti-inflammatory potential indicated a characteristic inhibition against COX-1 and COX-2 enzymes with IC50 62.8 and 14.23 µg/mL, respectively, compared to ibuprofen (IC50 8.07 and 6.58, respectively). Additionally, P. boisserei extract achieved a potent wound healing activity using in vivo rat model, this might be attributed to its high content of flavonoids together with other polyphenolic compounds that have a great free radical scavenging potential. P. boisserei is a promising candidate for more extensive phytochemical and biological exploration.

Aframomum Melegueta: Evaluation of Chronic Toxicity, HPLC Profiling, and In Vitro/In Vivo Antioxidant Assessment of Seeds Extracts.

Aframomum melegueta, commonly known as grains of paradise, is a medicinal plant celebrated for its rich phytochemical content and therapeutic properties. This study evaluated the antioxidant and cytotoxic potentials of its ethanolic and methanolic extracts, both in vitro and in vivo, while also analyzing their chemical profiles. HPLC analysis identified key compounds, including gallic acid, caffeic acid, caffeine, coumarin, rutin, catechin, ferulic acid, and quercetin. Chronic toxicity assessments confirmed the safety of the extracts, with no adverse effects on animal health, particularly in liver histopathology. Cytotoxicity results indicated reduced splenocyte viability at the highest concentrations. The extracts exhibited significant antioxidant activity in DPPH•, FRP, and phosphomolybdate assays, demonstrating their effectiveness as antiradical agents. In vivo antioxidant results showed a reduction in lipid peroxidation levels in serum and liver, highlighting the extracts' ability to mitigate oxidative stress. Additionally, the extracts provided protection against H2O2-induced erythrocyte hemolysis and modulated NO production in peritoneal macrophages. These findings underscore the therapeutic potential of A. melegueta extracts, suggesting their promise in developing preventive strategies for oxidative stress-related chronic diseases.

Synergistic Phytochemical and Nanotechnological Exploration of Melia azedarach With Silver Nitrate: Elucidating Multifaceted Antimicrobial, Antioxidant, Antidiabetic, and Insecticidal Potentials.

Plants are a rich source of bioactive compounds with significant pharmaceutical and health applications. This study explores the phytochemical, therapeutic, and phytotoxic properties of Melia azedarach by analyzing extracts from its bark, flowers, leaves, and fruits using six solvents: ethanol, methanol, acetone, hexane, chloroform, and distilled water. Twenty-one phytochemical tests were conducted, revealing significantly positive results for various tests. However, the ethanolic and methanolic flower extracts yielded no significant results in other tests. The highest total phenolic content was found in the chloroform extract of the leaves (96 ± 0.01 mg/100 g), and the highest antioxidant activity was observed in the ethanolic and hexane leaf extracts, with a 98% DPPH scavenging rate. Antibacterial testing showed significant efficacy against Serratia marcescens, Bacillus subtilis, Kluyvera spp., and Pseudomonas spp., with p values < 0.0001. The fruit chloroform extract demonstrated the highest alpha-amylase inhibition (93 ± 0.05), while the ethanolic leaf extract had the greatest tumor inhibition (85.6 ± 0.5). Insecticidal assays revealed that the acetone bark extract had the highest control values (56% and 57%). Due to their higher reducing potential, the leaves were used to biosynthesize silver nanoparticles (AgNPs), characterized by UV-Vis spectroscopy, EDX, and SEM, revealing an average particle size of 20-30 nm and spherical morphology. The AgNPs exhibited excellent antibacterial, antioxidant, antidiabetic, and insecticidal activities. These findings highlight the potential of M. azedarach and its AgNPs for developing novel therapeutic agents.

Antioxidative and Antimicrobial Activity of 2-Amino Substituted Halochalcone N-Glycoside Derivative Compounds

Introduction: The aim of this study was to specify the antioxidant and antimicrobial activity synthesized 2-amino substituted halochalcone N-glycoside derivative compounds. Methods: The antioxidant capacity of synthesized compounds (1-4) was determined by cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP). Antimicrobial activity was determined on 9 microorganism by agar well diffusion method.Results: According to the study results, compound 3 showed the highest antioxidant activity. The compound 3 showed antimicrobial activity against Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Candida albicans while compound 4 showed antimicrobial activity against Escherichia coli, Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Enterococcus faecalis, Bacillus cereus, Staphylococcus aureus.Conclusion: 2-amino substituted halochalcone N-glycoside derivative compounds could be evaluated in the pharmaceutical and cosmetic fields due to their antioxidant and antimicrobial potential.

Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi Artemisia absinthium L.

Background/Objectives: This study aimed to analyze the chemical composition and biological activities of Artemisia absinthium L. essential oil, focusing on its antioxidant and enzyme inhibition (α-amylase and urease) properties. Additionally, in vitro pharmacokinetic and pharmacodynamic evaluations were conducted through in silico molecular docking and BOILED-Egg models to assess its therapeutic potential and its potency in treating oxidative-stress-related diseases. Methods: The essential oil was isolated by the hydrodistillation (HD) of fresh plant material, and volatiles released from dried plant material were sampled via headspace solid-phase microextraction (HS-SPME), followed by a phytochemical profiling analysis through the GC-MS tool. Antioxidant capacity was assessed using DPPH, ABTS, FRAP, and nitric oxide scavenging assays, while enzyme inhibition activities were tested against α-amylase and urease. Molecular docking and BOILED-Egg models were used to evaluate compound interactions with NADPH oxidase and predict pharmacokinetic behavior, respectively. Results: HS-SPME and HD yielded 46 and 25 compounds, respectively, primarily terpenoids represented by camphor (26.4%) and cis-davanone (18.0%) in HS-SPME, while in the HD essential oil, cis-davanone (60.2%) and chamazulene (10.8%) were most prevalent. The antioxidant assays showed a strong activity, with a total antioxidant capacity of 821.8 mg ascorbic acid Eq/gm. The essential oil inhibited urease by 86.7% and α-amylase by 81.8%. Molecular docking showed strong binding affinities with NADPH oxidase, supporting the antioxidant results. Conclusions:A. absinthium EO demonstrated potent antioxidant and enzyme inhibitory activities, suggesting its therapeutic potential for treating enzyme-related disorders like diabetes mellitus and its possible use as a cure for many oxidative-stress-related diseases, thus validating the folkloric use of this plant.

The effect of Coix lachrymal L. seed extract on the expression of inflammation and fibrogenesis genes in human retinal pigment epithelial cells.

Proliferative vitreoretinopathy (PVR) is a vision-threatening condition associated with retinal-detachment (RD), primarily caused by fibrocellular scar membrane formation. This study investigates the therapeutic potential of adlay seed extract fractions in mitigating PVR-associated pathways, focusing on oxidative stress, proliferation, inflammation, and fibrogenesis in retinal pigment epithelial (RPE) cells. Adlay seed extract fractions (methanolic: MeOH and residual: Res) were obtained through solvent extraction and characterized for carbohydrate, protein, flavonoid content, and antioxidant activity. RPE cells were cultured, and their viability in response to adlay fractions was assessed using the MTT assay. Gene expression analysis of IL-1β, IL-6, LIF, TGF-β, Snail and α-SMA genes was conducted via real-time PCR after treatment with adlay fractions. The Res fraction exhibited higher levels of protein, carbohydrate, flavonoids, and phenols compared to the MeOH fraction, along with significantly enhanced antioxidant activity. Both fractions showed inhibitory effects on RPE cell viability, with the Res fraction demonstrating a more pronounced impact. Gene expression analysis revealed a significant decrease in IL-6 and TGF-β expression with the MeOH fraction treatment, while the Res fraction led to decreased expression of IL-6, LIF, TGF-β, Snail and α-SMA, indicating a more comprehensive modulation of PVR-associated pathways. This study highlights the potential therapeutic benefits of adlay seed extract fractions in mitigating PVR-associated pathways in RPE cells. The Res fraction, particularly rich in bioactive compounds and exhibiting potent antioxidant activity, shows promise in attenuating oxidative stress, proliferation, inflammation, and fibrogenesis, critical processes in PVR development. These findings underscore the potential of adlay seed extracts as a novel therapeutic strategy for PVR warranting further investigation and clinical validation.

The Effects of Enriching Shortbread Cookies with Dried Sea Buckthorn Fruit on the Physicochemical and Sensory Properties

The present study evaluated the physicochemical parameters, polyphenolics content, levels of heat-induced compounds (acrylamide, furfural, 5-hydroxymethylfurfural), antioxidant properties, as well as sensory quality of shortbread cookies enriched with dried sea buckthorn fruit (SBF). The SBF-enriched cookies were prepared by replacing 1, 2, 3, or 5% of the flour with dried sea buckthorn fruit. Our results showed the presence of 12 phenolic acids and 5 flavonoids in the SBF, while two phenolic acids and two flavonoids were detected in the cookies. Most of the compounds were identified in the cookies enriched with 5% SBF. Among the phenolic acids, benzoic acid was the most abundant, while among the flavonoids, quercetin was the most abundant. 5-hydroxymethylofurfural was not detected in any cookies, and the addition of SBF contributed to the presence of furfural in baked products and increased the amount of acrylamide, and their content increased with the amount of SBF addition. The addition of sea buckthorn fruit at 5% distinctly exceeded the benchmark level of acrylamide in the cookies and worsened their sensory quality. It should also be noted that SBF significantly (p &lt; 0.05) improved the antioxidant potential determined by two tests, ABTS and DPPH. The SBF-enriched cookies were characterized by significantly higher values of crispness and browning index compared to the control cookies. The results of the study indicate that SBF can be successfully used as a component of bakery products. In conclusion, the cookies with SBF can show improved technological and functional properties and constitute an added value bakery product that could provide health benefits.

Mitigating effects of Nigeria seaweed phytonutrients on biochemical and physiological conditions of Lycopersicon esculentum under heavy metal-induced abiotic stress

Abiotic stress has become a significant issue in recent years, affecting various physiological and biochemical aspects of crops and threatening food security. This study aimed to investigate the effects of seaweed on the biochemical and physiological conditions of Lycopersicon esculentum under heavy metal-induced abiotic stress. Lycopersicon esculentum plants were grown in greenhouse bags for four weeks and then exposed to 0.10M Pb Nitrate. They were divided into six groups: one control group sprayed with tap water and five experimental groups sprayed with different seaweed concentrations (0%, 20%, 40%, 60%, and 80%). Results showed that abiotic stress led to notable decreases in root length, shoot length, chlorophyll a, chlorophyll b, stomatal conductance, photosynthetic rate, Rubisco activity, and carotenoids by 48%, 47% 38%, 65%, 61%, 77%, 75%, and 68%, respectively. Seaweed treatment improved these indices, with the 80% seaweed group showing increases of 27%, 21%, 44%, 36%, 56%, and 48%, 54% after 21 days. Additionally, seaweed application enhanced antioxidant activities in the roots (SOD by 71%, CAT by 45%, POD by 51%, and APX by 92%) and in the leaves (SOD by 18%, CAT by 26%, POD by 34%, APX by 58%) compared to the control. The study concluded that seaweeds significantly mitigated oxidative stress in plants, boosting growth, antioxidant potential, mineral absorption, and osmotic protection. Nigeria seaweeds could be a promising tool to enhance food production by reducing oxidative stress in crops.

Rewiring the nexus between urban traffic pollution-derived polycyclic aromatic hydrocarbon exposure and DNA injury via urinary metabolomics

Urban road traffic environmental stress impacts outdoor population health, with oxidative damage serving as an early indicator of xenobiotic exposure. Polycyclic aromatic hydrocarbons (PAHs) as priority carcinogens pose significant public health burden, yet knowledge remains limited regarding the endogenous metabolic alternations associated with oxidative DNA injury. This cross-sectional study focused on the cohort consisting of 109 sanitation workers (“traffic exposure group”) and 112 demographics-matched common residents (“controls”) in South China. The goal was to elucidate the occurrence of internal exposure to nine hydroxyl PAHs, and the interrelations with oxidative DNA damage (indicated by 8-hydroxy-2′-deoxyguanosine, 8-OHdG) by linear mixed-effect regression model. T-test and orthogonal partial least squares discriminant analysis were used to determine differential metabolites in non-targeted metabolomics. Results revealed outdoor workers suffered from the heavier PAH exposure burden and exhibited a stronger dose-dependent correlation with 8-OHdG, evidenced by the higher regression coefficient (0.244, 95% CI: 0.154–0.334) than controls (0.203, 95% CI: 0.079–0.328). In total 42 differential endogenous metabolites witnessed significant expression under traffic emission scenario, mainly implicated in phenylalanine, tyrosine and tryptophan biosynthesis. The down-expressed uric acid was the unique metabolite that inversely correlated with the increased intake of ∑8PAH especially in cases. Partially attributed to the traffic-derived PAHs, the dysregulated amino acid, nicotinamide, purine, and steroid hormones metabolic pathways encompassing 11 metabolites were determined as underlying biomarkers in mediating DNA damage. Notably, our findings proposed uric acid may act as a potential antioxidant, as evidenced by the negative correlation with 8-OHdG. The study illustrates outcomes of metabolomics can collaboratively indicate DNA oxidative damage caused by PAHs linked to urban traffic exposure, which holds significant implications for future toxicological research.

Antidiabetic potential of Lavandula stoechas aqueous extract: insights into pancreatic lipase inhibition, antioxidant activity, antiglycation at multiple stages and anti-inflammatory effects

BackgroundWith the increasing global prevalence of type 2 diabetes (T2D) and obesity, there is a pressing need for novel therapeutic interventions. Lavandula stoechas, a medicinal plant traditionally used for various ailments, holds promise as a potential agent for T2D management, particularly in Morocco, where it is commonly used to treat diabetes. This study aims to evaluate the pharmacological potential of L. stoechas aqueous extract (AqLs) by assessing its lipase inhibition antioxidant and anti-inflammatory activities, identifying phenolic compounds, and examining its efficacy in reducing diabetic complications.MethodsThe pharmacological potential of L. stoechas aqueous extract was investigated using in vitro assays. The inhibitory effect on pancreatic lipase, antioxidant power (FRAP), and anti-inflammatory activity (albumin denaturation method) was assessed. High-performance liquid chromatography (HPLC) analysis identified phenolic compounds. Additionally, albumin glycation was evaluated by estimating fructosamine, carbonyl groups, and amyloid β-structures to assess efficacy in mitigating diabetic complications.ResultsThe extract demonstrated concentration-dependent inhibition of pancreatic lipase (IC50 = 0.132 ± 0.006 mg/mL), potent antioxidant activity (IC50 = 604.99 ± 1.01 μg/mL), and dose-dependent anti-inflammatory effects (IC50 = 207.01 ± 34.94 mg/mL). HPLC analysis revealed phenolic compounds: naringin (38.28%), syringic acid (25.72%), and cinnamic acid (15.88%) were the most abundant, with 4-hydroxybenzoic acid, hydrated catechin, and catechin ranging from 9.60% to 5.24%, and p-coumaric acid (1.73%). Furthermore, the extract inhibited albumin glycation and fructosamine production, suggesting efficacy in mitigating diabetic complications.ConclusionThese findings highlight the multifaceted pharmacological potential of L. stoechas aqueous extract in T2D management, suggesting that this plant can be highly beneficial for diabetic individuals.

Field and biochemical evaluation of glyphosate tolerant chickpea (Cicer arietinum L.) mutants developed through induced mutagenesis

Weed control in chickpea (Cicer arietinum L.) is challenging due to narrow genetic base of available germplasm and limited herbicide options. In this view, present research was focused on induced mutagenesis in chickpea for development of herbicide (glyphosate) tolerant mutants and subsequent screening under field conditions. Further, objective was to analyze the defence response and biochemical adjustments in selected glyphosate tolerant chickpea mutants. Initially, 376 chickpea mutants (M6 populations developed through EMS and gamma rays) were screened for glyphosate tolerance under filed conditions and scored on a 1 to 5 scale based on plant injury related traits. Among tested mutants, 40 were found highly tolerant (score = 5), 32 as tolerant (score = 4) and 20 as highly sensitive (score = 1) to glyphosate. Chickpea mutants with variable glyphosate tolerance also differed significantly (Tukey test, p < 0.05) in leaf biochemical profiles. For instant, lowest total oxidant status (4175.µM/g f. wt.) was detected in glyphosate tolerant mutant developed from desi chickpea genotype “D3009” using 0.3% EMS and in highly tolerant mutant (1775. µM/g f. wt.) developed from kabuli genotype “K709” using 0.2% EMS. In general, highly tolerant chickpea mutants exhibited highest antioxidant potential (SOD, POD, CAT, TAC) that contributed in glyphosate tolerance. Desi i.e. D1M1HT-2 and Kabuli i.e. KM3HT-2 type mutants with highest seed yield had maximum catalase activity (4200 Units/g f. wt and 540 Units/g f. wt.). Mutants developed from desi type genotypes were comparably superior to mutants derive from Kabuli in terms of herbicide tolerance.

The assessment of antioxidant potential of Olea europaea and Ocimum basilicum and their comparative and combine study

Many chronic diseases, such as cancer and diabetes, are connected to the negative consequences of reactive oxygen species (ROS). Ocimum basilicum or basil is a plant that is high in eugenol and vitamin K and is recognized for its antioxidant qualities. Research has indicated that basil possesses strong anticancer properties, impacting multiple biological processes like autophagy, cell cycle regulation, inflammation management, apoptosis, and metastasis suppression. Analogously, Olea europaea (olive) is acknowledged for its hypotensive, anti-inflammatory, and antioxidant characteristics. Because of their anticancer properties, the polyphenols included in olive leaves have demonstrated therapeutic efficacy against specific forms of cancer. At doses of 100, 500, and 1000 µg/mL, respectively, the Olea europaea extract demonstrated dose-dependent antioxidant activity with scavenging rates of 45%, 66%, and 83%. At the same concentrations, the Ocimum basilicum methanolic extract demonstrated antioxidant action as well, with scavenging rates of 11%, 43%, and 49%. Notably, both plants’ methanolic extracts showed increased antioxidant activity when combined. At doses of 100, 500, and 1000 µg/mL, the combined extracts demonstrated scavenging activities of 42%, 49%, and 71% using the DPPH free radical scavenging assay. This study's main goal is to assess the combined antioxidant capacity of methanolic extracts made from the leaves of Ocimum basilicum and Olea europaea. The results indicate that Ocimum basilicum and Olea europaea combination therapy considerably suppresses DPPH free radicals. With potential therapeutic effects, this combined therapy may be instrumental in scavenging free radicals that lead to the development of cancer.

In-vitro evaluation of antioxidant properties of aqueous extracts of Murraya koenigii, Aegle marmelos and Laurus nobilis

This study investigates the antioxidant properties of aqueous extracts of Murraya koenigii (curry leaf), Aegle marmelos (bael) and Laurus nobilis (bay leaf) using in vitro assays. The plants have been traditionally used in Indian medicinal systems for the treatment of various metabolic disorders. Aqueous extracts from the leaves of Murraya koenigii, fruit pulp of Aegle marmelos and leaves of Laurus nobilis were prepared. The total phenolic content and total flavanoid content was also determined in these aqueous extracts. They were tested for their ability to scavange free radicals by DPPH assay, ABTS assay and FRAP assay which are elevated during various metabolic disorders. The results suggest that all the three plants exhibited significant antioxidant activities. But strongest antioxidant properties were exhibited by Murraya koenigii leaf extract followed by the pulp of Aegle marmelos and lowest antioxidant activity was exhibited by Laurus nobilis extract, supporting their use in traditional medicine and potential development into therapeutic agents. So, this research suggested that these medicinal plants possess a significant antioxidant potential and are important source of natural antioxidants and can be effectively used in treating oxidative stress disorders. As a result, the study's significant findings demonstrate that it is a step towards evidence-based phytomedicine.