Antioxidant Potential Of Oil Research Articles

A Nano-Based Approach to Deliver Satureja thymbra Essential Oil to the Skin: Formulation and Characterization.

Essential oils are well known for their biological properties, making them useful for the treatment of various diseases. However, because of their poor stability and high volatility, their potential cannot be fully exploited. The use of nanoformulations to deliver essential oils can solve these critical issues and amplify their biological activities. We characterized an essential oil from Satureja thymbra via GC-MS and HPLC-DAD to provide qualitative and quantitative data. The essential oil was formulated in phospholipid vesicles which were characterized for size, surface charge, and storage stability. The entrapment efficiency was evaluated as the quantification of the major monoterpenoid phenols via HPLC-DAD. The morphological characterization of the vesicles was carried out via cryo-TEM and SAXS analyses. The essential oil's antioxidant potential was assayed via two colorimetric tests (DPPH• and FRAP) and its cytocompatibility was evaluated in HaCaT skin cell cultures. The results showed that the nanoformulations developed for the loading of S. thymbra essential oil were below 100 nm in size, predominantly unilamellar, stable in storage, and had high entrapment efficiencies. The vesicles also displayed antioxidant properties and high cytocompatibility. These promising findings pave the way for further investigation of the therapeutic potential of S. thymbra nanoformulations upon skin application.

Melissa officinalis essential oil as an antimicrobial agent against Listeria monocytogenes in watermelon juice

The burden of foodborne illness has a negative effect on public health, but also in countries' economy. Melissa officinalis is an aromatic plant known for its biological properties, including antioxidant and antimicrobial effects. This work highlighted M. officinalis essential oil's antioxidant potential and antimicrobial activity against L. monocytogenes, presenting a bactericidal action and being able to inhibit some virulence attributes, such as biofilm formation. The pre-exposure of the bacterium to subinhibitory levels of essential oil (0.125 μL/mL) did not induce high tolerance to stresses (such as high temperature, low pH, osmotic stress and desiccation) or cross-resistance with antibiotics, while not modifying the invasion ability to Caco-2 cells. When applied in food model media (lettuce, chicken and milk) and watermelon juice, the essential oil showed to have antimicrobial activity in a lettuce leaf model medium, further diminishing L. monocytogenes contamination and inhibiting the natural microbiota present in watermelon juice. M. officinalis essential oil shows potential to be used as control of L. monocytogenes in watermelon juice, while increasing the food's microbial shelf life.

Characteristics and Composition of Grape Seed Oil صفات ومکونات زیت بذور العنب

Grape seed oil is rich in phenolic compounds, fatty acids and vitamins which have high economic, and importance. It is used as an edible oil due to its beneficial properties for health, that are detected by many studies, such as anticancer, cardio protective and protective effect against cellular toxicity, induced by the environmental pollutants such as carbon tetrachloride (CCL4) . This pollutants generates free radical may cause a damage of the liver which is responsible for the metabolism of drugs and chemical toxic. The aim of this study was to detect the chemical composition of oil extracted from grape seeds (medium solvent) and antioxidant potential of oil by mixing with milk by product namely skim milk. Include the ameliorative effect against oxidative damage in cells induced by CCL4. The results of revealed that the extracted grape seed oil is rich in unsaturated fatty acids and low concentrations of saturated ones. Also, the resulting oil has a high antioxidant capacity incorporate with the product namely skim milk. In addition the incorporate of oil with skim milk has a prophylactic effect against the toxicity induced in liver by CCL4. On the other hand, enhance the activity of antioxidant enzymes namely Glutathione and Glutathione-S-Transferees. And antioxidant prosperity is claimed by a food model to be one of the mechanism of natural and hepato protective antioxidant.

GC/MS Profiling, In-Vitro Cytotoxic and Antioxidant Potential of the Essential Oil of Pulicaria Crispa (Forssk) Growing in Egypt

The essential oil EO obtained by hydrodistillation from the aerial parts of Pulicaria crispa was profiled using GS/MS analysis. The oil was found to contain carvotanacetone as a major component (81.9%). Biological investigation revealed that the oil possessed EC100 value of12.8 µl/ml in the in-vitro cytotoxicity assay against peripheral blood mononuclear cells (PBMCS). The oil showed no immune-stimulant activity. The antioxidant potential of the oil was assayed using DPPH radical inhibition resulting in inhibition percentage of 66.19% and trolox equivalent antioxidant capacity (TEAC) of 9.22µg/ml. The results indicate powerful antioxidant capacity of the oil, which makes it a good candidate to be used as a natural preservative or as a source of natural antioxidants to be incorporated in pharmaceutical applications. The cytotoxicity assay of the oil against colorectal“Caco-2” and hepatocellular “HepG-2” carcinoma cell lines showed IC50 values of 4.73 and 20.11 µl/ml, respectively. The obtained results spot the light on the colorectal anticancer potential of the oil, which should be subjected to future animal experimental study in form of enema.

Chemical Composition, Antioxidant and Anti-bacterial Potential of Essential Oil of Medicinal plant Isodon rugosus

Isodon rugosus is a medicinally valuable herb containing a variety of important secondary metabolites and essential oil with important chemical constituents of pharmaceutical precursors. The active components of volatile oil of I. rugosus were analyzed using GC-MS. Anti-oxidant potential of the oil was assessed with 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Anti-bacterial power of the essential oil accessed disc diffused method. A total of 19 constituents were quantified and identified with different concentrations in essential oil. Among which Caryophyllene oxide was 28.99%. Limonene oxide cis was 26.52%, (Z, E)-Farnesol was 17.34%, followed by 1-erpinen-4-ol 3.33%, o-Cymene 2.04 while the concentrations of rest of constituents were between 1.11 to 0.2%. The investigation showed that the essential oil of I. rugosus possessed significant antibacterial and antioxidant potential

Trehalose‐Induced Changes in Seed Oil Composition and Antioxidant Potential of Maize Grown Under Drought Stress

AbstractThe present investigation was conducted to determine if foliar‐applied trehalose (an osmoprotectant) could ameliorate the adverse effects of water shortage on maize seed oil composition and oil antioxidant potential. Drought stress significantly reduced the seed oil but increased oleic acid and linolenic acid contents of the oil with a concomitant decrease in linoleic acid content, which resulted in an increased oil oleic/linoleic ratio in both maize cultivars. Water stress also increased the seed oil α‐ γ‐ δ‐ and total tocopherols and flavonoids of both maize cultivars, however, oil phenolic content and oil antioxidant activity decreased. Exogenously applied trehalose positively influenced seed composition of both maize cultivars under non‐stress and water stress conditions. Exogenous application of trehalose further increased the oil oleic and linolenic acid contents with a subsequent decrease in linoleic acid. Furthermore, exogenous application of trehalose increased the oil antioxidant activity in terms of oil DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) free radical scavenging activity with an increase in oil tocopherols, total flavonoids and total phenolics contents.

Exogenously applied glycinebetaine enhances seed and seed oil quality of maize (Zea mays L.) under water deficit conditions

A field trial was carried out to appraise up to what extent exogenous application of a potential osmoprotectant, glycinebetaine (GB), could ameliorate the inhibitory effects of shortage of water on maize seed and seed oil composition and oil antioxidant potential. Two maize cultivars, Agaiti-2002 (drought tolerant) and EV-1098 (drought sensitive), were exposed to drought treatments at the vegetative growth stage. Both the maize cultivars used in the present study are being widely cultivated in Pakistan and have been an important source of developing different maize hybrids. Two levels of glycinebetaine (0 or 30mM) were foliar-applied at the vegetative stage. Water stress reduced the kernel sugar, oil, protein, moisture contents and most of the seed micro- and macro-nutrients analyzed of both maize cultivars, but it increased the contents of seed fiber and ash contents. Among different seed oil un-saturated fatty acids, water stress increased the oil oleic acid contents with a decrease in linoleic acid contents, which resulted in increased oil oleic/linoleic ratio of both maize cultivars. However, no variation was observed in oil stearic and palmitic acid contents due to water stress. A considerable increase in seed oil α-, γ-, δ- and total tocopherols and flavonoids was observed in both maize cultivars. However, oil phenolic content and 1,1′-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity decreased. Foliar-applied GB significantly increased the contents of seed sugar, oil, protein, moisture, fiber, ash, GB contents and micro- and macro-nutrients of both maize cultivars under well irrigated and water deficit conditions. Furthermore, exogenous application of GB increased the oil oleic and linoleinic acid contents. All different lipophilic compounds estimated in the seed oil increased due to foliar applied GB. Furthermore, GB also increased seed oil antioxidant activity appraised in terms of oil DPPH free radical scavenging activity. By summarizing the results, it seemed that exogenously applied GB remained in intact form until later stages of growth and counteracted the inhibitory effects of water deficit on seed and seed oil composition similarly of both maize cultivars.