Antioxidant Potential Of Hydroalcoholic Extract Research Articles

Pharmacognostical, phytochemical and antioxidant potential of hydroalcoholic extract of lam leaves

Medicinal plants have bioactive compounds which are used for curing of various human diseases and also play an important role in healing. Secondary constituents contain alkaloids, flavonoids, phenol, saponin, steroids and tannins. Medicinal plants have anticancer, antimicrobial, antidiabetic, antidiuretic and anti- inflammation activities ect. The increasing interest in powerful biological activity of secondary metabolites outlined the necessity of determining their contents in medicinal plants. Lam () is a fast-growing and drought-resistant tree of the family. The tree is known with some common names: miracle, ben oil, drumstick, horseradish or simply moringa. Phytochemical studies of plant organs showed the plant as a rich source of primary and secondary metabolites belonging to different classes of organic compounds. Pharmacological studies confirmed the use of the plant to cure several diseases and to possess nutraceutical properties. The objective of this study was to investigate pharmacognostical, phytochemical features and antioxidant activity of hydroalcoholic extracts of leaves by using DPPH assay method. The different pharmacognostical parameters were evaluated as per standard protocols with some modifications. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The antioxidant activity of hydroalcoholic extract of the leaves was assessed against DPPH method using standard protocols.Phytochemical analysis revealed the presence of alkaloids, glycosides, flavonoids, tannins, amino acid and carbohydrate. The total phenolic and flavonoids content of leaves of hydroalcoholic extract was 0.864and 1.014 mg/100mg respectively. The activities of hydroalcoholic leaves extract against DPPH assay method were concentration dependent with IC values of ascorbic acid and extracts 17.68 and 79.10µg/ml respectively. The present study concluded that the crude extract of is a rich source of secondary phytoconstituents which impart significant antioxidant potential. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.

Antipyretic and Anti-oxidant potential of Hydroalcoholic extract of Gendarussa vulgeris

G. vulgaris Nees of the family Apocynaceae is a medium sized tree grown in semishade or no shade and is common in the Ernad and Nilambur taluks of Kerala. Various parts of this plant have been used in the treatment of ulcers, sores, inflammation, dyspepsia, healing of wounds, etc. The present study aimed at the evaluation of anti-pyretic activity of the hydroalcoholic extracts of the leaves by in vivo methods. Phytochemical screening reveals the presences of Alkaloids, Saponins, Carbohydrates, Flavonoids and Phenols. The total phenolic content was found 0.691mg/100mg of dry weight of extract, expressed as gallic acid equivalents and the total flavonoid content was found 0.847mg/100mg, expressed as Quercetin equivalents. Antioxidant activity was performed using DPPH method. The IC50 value of Hydroalcoholic extract of Gendarussa vulgaris was found to be 55.79µg/ml. Yeast induced pyrxia and anti-pyretic property of hydroalcoholic extract of leaves of Gendarussa vulgaris. The effect of hydroalcoholic extract of leaves of Gendarussa vulgaris were determined after administration at two dose levels (100 and 200 mg/kg b.w.) in yeast induced pyrxia rats. From the results, it may be concluded that hydroalcoholic extract of leaves of Gendarussa vulgaris possess significant anti-pyretic effect may be due to the effect of antioxidants and constituent present in the leaves.

Extraction, Phytochemical Screening and Antioxidant Potential of Hydroalcoholic Extract of Viola odorata Linn

Bioactive chemicals found in medicinal plants are used to treat a variety of human ailments and serve a vital part in healing. Alkaloids, flavonoids, phenol, saponin, steroids, and tannins are examples of secondary components. Anticancer, antibacterial, antidiabetic, anti-diuretic, and anti-inflammation properties are all found in medicinal plants. The growing interest in secondary metabolites' significant biological action has highlighted the need of assessing their presence in therapeutic plants. Viola odorata (Viola odorata, Violaceae) is a Viola species native to Europe and Asia that has recently been introduced to North America and Australasia. In India, the plant is known as Banafsa, Banafsha, or Banaksa, and it's often used to treat sore throats and tonsillitis. It was known to be used to treat cancer-related pain. It has traditionally been used to treat anxiety, sleeplessness, and high blood pressure. Alkaloids, glycosides, saponins, methyl slicylate, mucilage, and vitamin C are all found in it. Antioxidant, analgesic, antihypertensive, and diuretic properties have been documented for the plant. The goal of this research is to identify phytochemicals, assess phenolic and flavonoid content, and measure the antioxidant potential of V. odorata flowers. The well-known test methodology was used to determine qualitative analysis of various phytochemical elements as well as quantitative analysis of total phenol and flavonoids. The antioxidant activity of a hydroalcoholic extract of V. odorata flowers was tested in vitro using the 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay and the hydrogen peroxide radical scavenging technique. Phenols, flavonoids, tannins, saponins, alkaloids, and other phytochemicals were discovered by phytochemical investigation. The total phenolic and flavonoids content of the hydroalcoholic extract of V. odorata flower was 0.521 and 0.637 mg/100 mg, respectively. In the investigated models, the extract showed dose-dependent free radical scavenging properties. For the DPPH technique, the IC50 value of V. odorata floral extract was 109.78g/ml, which was close to that of ascorbic acid (IC50=27.82g/ml). The IC50 value for hydrogen peroxide was discovered to be 85.85g/ml, which compares well to ascorbic acid (IC50=16.48g/ml). The present study describes the phytochemical profile and antioxidant activity of V. odorata flower which will further used for medicinal applications.

Extraction, Phytochemical Screening and Antioxidant Potential of Hydroalcoholic Extract of Aerial Parts of Bauhinia variegata

The goals of this research are to screen phytochemicals, perform thin layer chromatography, assess the quantity of phenolic and flavonoid compounds, and measure the antioxidant potential of Bauhinia variegata. The well-known test methodology available in the literature was used to determine the qualitative analysis of various phytochemical elements as well as the quantitative analysis of total phenol and flavonoids. The hydro alcoholic extract of Bauhinia variegata aerial parts was investigated for qualitative and quantitative analyses, as well as antioxidant activity using an in vitro model, namely the 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay. The presence of phenols and flavonoids was discovered by phytochemical research. The total phenolic and flavonoid content of Bauhinia variegata hydroalcoholic extract was 1.454 and 1.112mg/100mg, respectively. Ascorbic acid, which was employed as a control, was also assessed for comparison. In the investigated models, the extract demonstrated dose-dependent free radical scavenging activity. Bauhinia variegata aerial parts For the DPPH technique, hydroalcoholic extract had an IC50 value of 60.22g/ml, which was close to that of ascorbic acid (IC50=17.68g/ml). The IC50 value for the hydrogen peroxide technique was determined to be 76.97g/ml, which compares favourably to ascorbic acid (IC50=18.69g/ml). The current study discusses the phytochemical profile, antioxidant activity, and TLC of Bauhinia variegata, which will be employed for medical purposes in the future.

Evaluation of Phytochemical constituents and Antioxidant potential of hydro-alcoholic extract of seeds of Petroselinum crispum

Evaluation of Phytochemical constituents and Antioxidant potential of hydro-alcoholic extract of seeds of Petroselinum crispum

Botanical standardization, phytochemical analysis, and antioxidant studies of various fractions of Atibala [Abutilon indicum (L.) Sweet] leaves

BACKGROUND: Abutilon indicum (L.) Sweet (family: Malvaceae) is a perennial herb with golden yellow flowers called as Atibala in Ayurveda. The roots of this plant were widely used in traditional system of medicine as aphrodisiac, uterine tonic, and leaves are used in bronchitis, gonorrhea, fever, and urethritis. AIM: In this study, histological, powder microscopical studies of A. indicum (Malvaceae) leaves, followed by total phenolic, flavonoid content, antioxidant potential of hydroalcoholic extract, and its fractions, were determined. MATERIALS AND METHODS: Anatomical, powder microscopical studies were carried out according to the Ayurvedic Pharmacopoeia of India. Hydroalcoholic extract was subjected to fractionation with different solvents, performed phytochemical studies for all fractions, and screened for DPPH (diphenyl picrylhydrazyl) radical scavenging activity and reducing power capacity. RESULTS: The phytochemical screening revealed the presence of phenolic and flavonoid compounds in all the fractions. The total phenolic content of hydroalcoholic/mother extract and all fractions was ranged from 20 to 40 mg GAE/g. The flavonoid content of mother extract and all fractions was measured; values ranged from 16 to 30 mg RE/g. The highest DPPH radical scavenging activity was demonstrated by ethyl acetate fraction (IC50-60 µg/mL), followed by butanol fraction (IC50-95 µg/mL). The reducing powers of all the extracts were comparable with those of positive control butylated hydroxyl toluene (BHT). CONCLUSION: The high content of phenolic compounds indicated that these compounds are responsible for antioxidant activity. Therefore, ethyl acetate fraction of A. indicum leaves can be considered as a promising candidate for natural plant sources of antioxidants.

Assessment of In-Vivo Antioxidant Potential of Hydro-Alcoholic Extract and Ethyl Acetate Fraction of Aerva Javanica Linn. Flowering Tops

Aerva javanica (Amaranthaceae) is a grey coloured woolly perennial tomentose shrub. Its traditional and folklore usage motivates further investigation on its pharmacognostic parameters and pharmacological potential. Hydro-alcoholic extract (AJCE) was prepared from flowering tops of A. javanica. In order to work further on activity guided fractions, ethyl acetate (AJEAF) fraction was prepared. Therefore, in order to establish its antioxidant potential, in-vivo effect on LPO, GSH, SOD and catalase activity was determined. For comparison, silymarin and Centella asiatica extract (CAE) were used as standard antioxidant compound/extract. Lipid peroxidation in term of MDA content expressed as nM/mg, which was 82.18 and 67.39 for AJCE with increasing doses of complete hydro-alcoholic extract (AJCE represented as AJCE-1 and AJCE-2) and 51.65 for AJEAF in contrast to 40.64 nM/mg for standard silymarin and 46.81 nM/mg for standard CAE. GSH content was determined as 3.12, 3.82 and 4.56 μg/mg wet tissue in contrast to 5.59 for standard silymarin and 4.42 for standard CAE. Superoxide scavenging was expressed as SOD U/mg wet tissue, determined as 7.26, 9.16 and 9.91 U/mg wet tissue for AJCE-1 (250 mg/kg i.p. b.w), AJCE-2 (500 mg/kg i.p. b.w), and AJEAF respectively in comparison to silymarin (10.11) and CAE (46.81 U/mg wet tissue). Catalase activity expressed as μM of H2O2 decomposed / min / mg wet tissue was determined as 0.61, 0.72 and 0.78 repectively for AJCE-1 (250 mg/kg i.p. b.w), AJCE-2 (500 mg/kg i.p. b.w), and AJEAF. Results indicated the SOD values and total antioxidant power of DEE and EAF fractions even better than standard ascorbic acid which expressed the prospective potential of fractions (DEE and EAF) against metabolic disorders.

Isolation and Characterization of Volatile Oil by Gc-Ms Analysis and Screening of In Vitro Antioxidant Potential of Hydroalcoholic Extracts of Some Indigenous Plants of Northern India in Rodents

The main aim and objective of my research work is the isolation of volatile oil from the leaves of plants by GC-MS analysis and screening of in vitro antioxidant potential of hydroalcoholic extracts of some Indigenous plants of Northern India in rodents. To perform this research work the fresh leaves of Prunus persica, Calotropis procera & Canscora decussata were taken. The isolation of volatile oil was done by using Clavenger apparatus. The samples were further analyzed by Gas Chromatography-Mass Spectrometry. In vitro antioxidant potential was carried out by DPPH, Hydrogen peroxide, Hydroxyl radical and Nitric oxide scavenging activity. Ascorbic acid was used as standard drug in this study. The percent inhibition values for antioxidant potential were obtained and tabulated. The present experimental data clearly displayed that the hydroalcoholic extract of above mentioned plant’s leaves exhibited antioxidant potential.

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities.
 Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

Phytochemical screening and in vitro antioxidant potential of hydroalcoholic extract from the aerial parts of bryonopsis laciniosa

Phytochemical screening and in vitro antioxidant potential of hydroalcoholic extract from the aerial parts of bryonopsis laciniosa

Flavonoids and antioxidant potential of nine Argentinian species of Croton (Euphorbiaceae)

Croton is a large genus of Euphorbiaceae and many of its species are used medicinally. This study was performed to analyze the flavonoid composition and the antioxidant potential of hydroalcoholic extracts of nine Argentinian species of Croton: C. andinus Mull. Arg.; C. argentinus Mull. Arg.; C. catamarcensis Ahumada; C. cordobensis Ahumada; C. curiosus Croizat; C. lachnostachyus Baill.; C. lanatus Lam. var. lorentzii (Mull. Arg.) P.E. Berry; C. saltensis Griseb.; and C. serratifolius Baill. Few investigations studying the analyses of flavonoid glycosides in Croton have been carried out. The hydroalcoholic extracts (80 % methanol) from leaves were analyzed by high-performance liquid chromatography–mass spectrometry. The antioxidant activity was analyzed by the 1,1-diphenyl-2-picryl-hidrasil, Sigma (DPPH), ferric reducing antioxidant potential (FRAP), and Folin–Ciocalteu methods. O-Glycosides of flavonols (kaempferol, quercetin, and isorhamnetin) and flavones (apigenin) were ubiquitous in the analyzed samples. Rutin was frequent and predominant, especially in Adenophylli section. Acylglycosides of either quercetin or kaempferol (tiliroside) were detected in most samples. C. argentinus and C. serratifolius were the species with the highest antioxidant capacity and the highest total content in proanthocyanidins and total phenols together. Significant degree (P < 0.05) of correlation was found between relative abundances of total phenols, proanthocyanidins, and quercetin derivatives, with FRAP and DPPH. Principal Component analysis showed a close affinity among Adenophylli species.

Antioxidant potential and its relationship with polyphenol content and degree of polymerization in Opuntia elatior Mill. fruits.

Background:Opuntia elatior Mill. (Nagaphani) fruits are traditionally recommended as an expectorant, remedy for whooping cough, asthma, gonorrhea, ulcers, tumors, in the treatment of diarrhea and syphilis. Many of these diseases are allied with oxidative stress caused by free radicals. Thus, current research is directed towards finding naturally-occurring antioxidants of plant origin.Aim:To evaluate antioxidant potential of hydro-alcoholic extract of the O. elatior fruits (HAOE) and its fractions.Materials and Methods:Using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay, total polyphenolic, flavonoid (FA), flavanone (FO) contents and degree of polymerization in relation with its antioxidant activity were examined.Results:The experimental data indicated that the HAOE, ethyl acetate (EAOE) and butanol (BFOE) soluble fractions have shown significant antioxidant activity. The highest polyphenolic, FA, FO contents and degree of polymerization were found in EAOE. The scavenging potential was in the order of Ascorbic Acid > EAOE > BFOE > HAOE > BIOE, where ascorbic acid was used as a positive control. The increased antioxidant potential of EAOE and BFOE fractions over HAOE extract may be attributed to the purification achieved by fractionation of the extract which in turn resulted in an increase in the degree of polymerization and segregation of secondary metabolites.Conclusion:The fruit of O. elatior can be used as the best alternative for synthetic antioxidants.