Antioxidant Potential Of Fractions Research Articles

Antioxidant, Toxicity and Secondary Metabolites Contents of Ethylacetate Fraction from Soft Coral Lobophytum Sp. Growing in South East Sulawesi

Soft coral Lobophytum sp is widely distributed in the waters of South East Sulawesi, Indonesia. However, no research data on the chemical and medicinal aspects of this genus from this region have been published. Therefore, this article aims to describe the findings of those aspects of Lobophytum sp. from this region. Ethyl acetate was used to extract the sample, and Vacuum liquid chromatography (VLC) was used to fractionate the ethyl acetate extract. The DPPH radicals and ABTS methods were used to assess antioxidant potency, the BSLT method was used to evaluate toxicity, and the Phytochemical test and LCMSMS method were used to determine the chemical composition. The results showed that the ethyl acetate extract was produced in seven fractions namely Fraction A-G. The weight of each extract was A (12.8% w/w), B (9.7%), C (10.1%), D (2.0%), E (7.0%), F (25, 3%) and G (11.5%). Further studies were carried out on the fraction F which was the fraction with the highest weight. Based on the level of antioxidant power proposed by Blois, the antioxidant potential of fraction F is a strong category with an IC50 value (μg/mL) 99.13 (DPPH) and 79.30 (ABTS). Fraction F is also not toxic with LC50 198.21 μg/mL. Those activities were supported by the qualitative phytochemical screening that exhibited the extract contains phenolic compounds. LCMSMS data indicated that fraction F of ethyl acetate extract contains fragransol D, lirioresinol A, neociwujiaphenol, phillygenin, pluviatelol, saurufuran B, β-hydroxyisovalerylshikonin and some unidentified compounds with molecular formulas C21H26O8, C23H26O8, C19H24O6.

Comparative Phytochemical, Antioxidant, and Hemostatic Studies of Extract and Four Fractions from Paulownia Clone in Vitro 112 Leaves in Human Plasma

Background: The Paulownia Clone in Vitro 112, known as oxytree or oxygen tree, is a hybrid clone of the species Paulownia elongata and Paulownia fortunei (Paulowniaceae). The oxytree is a fast-growing hybrid cultivar that can adapt to wide variations in edaphic and climate conditions. In this work, Paulownia Clone in Vitro 112 leaves were separated into an extract and four fractions (A–D) differing in chemical content in order to investigate their chemical content using LC-MS analysis. The extract and fractions were also evaluated for their anticoagulant and antioxidant properties in a human plasma in vitro. Results: The Paulownia leaf extract contained mainly phenolic compounds (e.g., verbascoside), small amounts of iridoids (e.g., aucubin or 7-hydroxytometoside) and triterpenoids (e.g., maslinic acid) were also detected. Our results indicate that the extract and fractions have different effects on oxidative stress in human plasma treated with H2O2/Fe in vitro, which could be attributed to differences in their chemical content. For example, the extract and all the fractions, at the two highest concentrations of 10 and 50 µg/mL, significantly inhibited the plasma lipid peroxidation induced by H2O2/Fe. Fractions C and D, at all tested concentrations (1–50 µg/mL) were also found to protect plasma proteins against H2O2/Fe-induced carbonylation. The positive effects of fraction C and D were dependent on the dose. Conclusions: The extract and all four fractions, but particularly fractions C and D, which are rich in phenolic compounds, are novel sources of antioxidants, with an inhibitory effect on oxidative stress in human plasma in vitro. Additionally, the antioxidant potential of fraction D may be associated with triterpenoids.

Antileishmanial and antioxidant potential of fractions and isolated compounds from Nectandra cuspidata

The effects on the viability of promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and citotoxicity in J774.A1 macrophages of ethanolic extract, fractions and isolated compounds that were obtained from the leaves of Nectandra cuspidata were determined. The total phenolics content (TP) and antioxidant activity were assessed using different assays. The ethyl acetate fraction (EAF) had the best antiproliferative activity (amastigote IC50: 4.42 ± 0.60 μg/mL, promastigote IC50: 33.33 ± 0.78) and presented no cytotoxicity at the highest concentration tested (1000 μg/mL). The EAF also had the greatest antioxidant capacity, which was comparable to butylated hydroxytoluene and quercetin (DPPH IC50 6.54 ± 0.10 μg/mL, ABTS 4.75 ± 0.11 mmol ET/g; FRAP 2.37 ± 0.03 mmol ET/g; ORAC = 33.52 ± 1.99 mmol ET/g; TP 387.11 ± 0.25 mg GAE/g). Vitexin, epicatechin and isovitexin isolated from EAF contribute to both activities.

IN VITRO EVALUATION OF ANTIOXIDANT ACTIVITY OF SKIMMIA ANQUETILIA LEAVES EXTRACTS

The purpose of the present study is to evaluate in vi tro antioxidant and free radical scavenging potential of different organic and aqueous fraction s of Skimmia anquetilia Tayl . Initially the phytochemical constituent s of the plant were extracted in methanol and then methanol ic extract was dissolved in dist. water and fractionated with n - hexane , dichloromethane , ethyl acetate and n - butanol respectively . E ight method s were use d for the determination of antioxidant potential of the fractio n s such as “ 2,2 ' - azinobis(3 - ethylbenzothiazoline - 6 - sulpohonic acid) (ABTS) radical cation scavenging ” , the “ ferric reducing antioxidant power (FRAP) ” , “ 2,2´ - diphenyl - 1 - picrylhydrazil (DPPH) radical scavenging ” , “ total phenolic content s (TPC) ” , “ total flavo noid content s (TFC) ” , “ total a ntioxidant activity by phosp homolybedenum method ” , “ su peroxide anion radical scavenging activity ” and “ metal chelating activity ” . The result s show ed that in ABTS assay the t rolox equivalent antioxidant capacity (TEAC) values f or different fractions ranged from 8.87 - 0 .95 µg/ml. EtOAc fraction showed highest activity as compared to other fractions, having EC 50 value 2.87 µg/ml. It also exhibited highest FRAP value ( 146.28 mM / m L FeSO 4 equivalents) as well as highest to tal phenolic contents (3.04 mg/ml GAE) and highest DPPH radical scavenging activity. The remaining aqueous fraction exhibited highest superoxide anion radical scavenging a ctivity ( 99.5 %) . Dichloromethane fraction showed highest total antioxidant activity i.e. 0.55 ± 0.25. Crude methanolic extract ex hibited highest total flavonoid contents (1698 mg/g quercetin equivalents ) as well as highest metal chelating activity 73.5 % . The amass results of different assay s show ed that S. anquetilia is affluent source of antioxidan t s .