Antioxidant, Toxicity and Secondary Metabolites Contents of Ethylacetate Fraction from Soft Coral Lobophytum Sp. Growing in South East Sulawesi

Abstract

Soft coral Lobophytum sp is widely distributed in the waters of South East Sulawesi, Indonesia. However, no research data on the chemical and medicinal aspects of this genus from this region have been published. Therefore, this article aims to describe the findings of those aspects of Lobophytum sp. from this region. Ethyl acetate was used to extract the sample, and Vacuum liquid chromatography (VLC) was used to fractionate the ethyl acetate extract. The DPPH radicals and ABTS methods were used to assess antioxidant potency, the BSLT method was used to evaluate toxicity, and the Phytochemical test and LCMSMS method were used to determine the chemical composition. The results showed that the ethyl acetate extract was produced in seven fractions namely Fraction A-G. The weight of each extract was A (12.8% w/w), B (9.7%), C (10.1%), D (2.0%), E (7.0%), F (25, 3%) and G (11.5%). Further studies were carried out on the fraction F which was the fraction with the highest weight. Based on the level of antioxidant power proposed by Blois, the antioxidant potential of fraction F is a strong category with an IC50 value (μg/mL) 99.13 (DPPH) and 79.30 (ABTS). Fraction F is also not toxic with LC50 198.21 μg/mL. Those activities were supported by the qualitative phytochemical screening that exhibited the extract contains phenolic compounds. LCMSMS data indicated that fraction F of ethyl acetate extract contains fragransol D, lirioresinol A, neociwujiaphenol, phillygenin, pluviatelol, saurufuran B, β-hydroxyisovalerylshikonin and some unidentified compounds with molecular formulas C21H26O8, C23H26O8, C19H24O6.