Antioxidant Of Extracts Research Articles

Assessment of antioxidant potential, cytotoxicity, and anticancer activity of methanolic extracts from selected wild medicinal plants

IntroductionMedicinal plants are considered an important source of human health because of their therapeutic capabilities in treating various diseases. This study aimed to evaluate the phytochemical profile, antioxidant, and cytotoxic activities of the methanolic extracts of three wild medicinal plants (Ruta graveolens, Peganum harmala and Citrullus colocynthis). MethodsThe total phenolic content was determined according to the Folin–Ciocalteu method. The total flavonoid contents were measured by the aluminum chloride colorimetric method. The antioxidant activity was estimated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays. The phytochemical analysis was performed using liquid chromatography-mass spectrometry method. The cytotoxicity and anticancer activity of each plant extract was investigated against fibroblast and MDA-MB-231 breast cancer cell lines, respectively, using Giemsa staining assay. ResultsR. graveolens extract exhibited the highest concentrations of total phenols (25.5 ± 0.5 mg GA/g dry extract) and flavonoids (9.8 ± 0.5 mg Rutin/g dry extract). Additionally, it demonstrated the most robust antioxidant capacity, as demonstrated by both DPPH (100.8 ± 0.56 mg GA/g dry extract) and FRAP (91.2 ± 1.33 mg GA/g dry extract) assays. Within this plant, Rutin constituted 13.7 % of the total compounds. Furthermore, R. graveolens displayed the most significant anticancer activity, with statistical significance at p< 0.01. Notably, it exhibited the lowest cytotoxicity against the fibroblast cell line, even at the highest tested concentration of 100 μg/ml. In contrast, both P. harmala and C. colocynthis demonstrated substantial anticancer potential (p< 0.01); however, they both exhibited significant cytotoxicity against the fibroblast cell line (p<0.01). ConclusionR. graveolens extract holds promise as a potential reservoir of safe and effective antioxidant and anticancer compounds for human use.

Antioxidant, Immunomodulatory and Fungicidal Potential of Different Extracts of Raphanus Raphanistrum L. Var. Caudatus

Background: Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases, emphasizing the importance of antioxidants in maintaining cellular homeostasis. Raphanus raphanistrum, commonly known as wild radish, has been traditionally used for its medicinal properties, including antioxidant, immunomodulatory, and antifungal effects. The plant is rich in bioactive compounds such as flavonoids, isothiocyanates, and vitamins, which contribute to its therapeutic potential. Objective: This study aimed to evaluate the antioxidant, immunomodulatory, and antifungal activities of Raphanus raphanistrum extracts and to compare these activities with standard controls. Methods: Fresh Raphanus raphanistrum plants were collected, and extracts were prepared using methanol, chloroform, and water. The antioxidant activity was assessed using the DPPH radical scavenging assay. Immunomodulatory activity was evaluated through the luminol-enhanced chemiluminescence assay in whole blood and polymorphonuclear leukocytes (PMNS), measuring the oxidative burst. The antifungal activity was determined using the agar disk diffusion method against six pathogenic fungi. The statistical analysis was performed using SPSS version 25, with results presented as mean ± standard deviation for antioxidant and antifungal assays, and mean ± standard error for immunomodulatory activity. Results: The methanol extract exhibited significant antioxidant activity with DPPH scavenging percentages ranging from 56.55% at 150 µg/ml to 87.08% at 850 µg/ml. Immunomodulatory activity showed IC50 values of 37.8 µg/ml in whole blood and 18.9 µg/ml in PMNS for the methanol extract. The antifungal activity against Candida albicans showed zones of inhibition increasing from 15 mm at 150 µg/ml to 22 mm at 850 µg/ml. These activities were compared to standard controls, including ascorbic acid for antioxidant, ibuprofen for immunomodulatory, and miconazole for antifungal assays, demonstrating the extracts' competitive potential. Conclusion: Raphanus raphanistrum extracts showed promising antioxidant, immunomodulatory, and antifungal activities, indicating their potential as natural alternatives for the prevention and treatment of related diseases. Further in vivo studies and isolation of active compounds are recommended to fully understand the mechanisms and therapeutic applications.

HPLC-MS/MS Profiles, Antioxidant, Neuroprotective, Antidiabetic and Skin Protective Effects of Different Extracts of Vicia peregrina L. Collected from the Eastern Region of Turkey.

Many Vicia species (Fabaceae) were proven to possess bioactive compounds with potential health beneficial properties. The present study was designed to determine the phenolic constituents, antioxidant and enzyme inhibition activities of aerial parts and seed of V. peregrina. Hexane, ethyl acetate and methanol extracts were prepared by maceration and aqueous extract by infusion. The chemical compositions of the extracts were determined using HPLC-MS/MS technology. The antioxidant activities were examined using various assays including free radical scavenging (ABTS and DPPH), reducing ability (CUPRAC and FRAP), metal chelation, and phosphomolybdenum. The enzyme inhibitory effects were investigated against cholinesterase, tyrosinase, amylase and glucosidase. The highest total phenolics and flavonoids contents were recorded in the methanol extracts of the seed (45.42 mg GAE/g) and aerial parts (40.33 mg RE/g) respectively. The aerial parts were characterized by higher accumulation of chlorogenic acid (9893.86 μg g-1 ), isoquercitrin (9400.33 μg g-1 ), delphindin 3,5 diglucoside (9113.28 μg g-1 ), hyperoside (6337.09 μg g-1 ), rutin (3489.83 μg g-1 ) and kaempferol-3-glucoside (2872.84 μg g-1 ). Generally, the methanol and aqueous extracts of the two studied parts exerted the best antioxidant activity with highest anti-DPPH (61.99 mg TE/g), anti-ABTS (101.80 mg TE/g) and Cu++ (16169 mg TE/g) and Fe+++ (172,36 mg TE/g) reducing capacity were recorded from the seed methanol extract. Methanol extract of the seed showed the best anti-tyrosinase activity (75.86 mg KAE/g). These results indicated that V. peregrina is rich with bioactive phenolics suggesting their use in different health promoting applications.

Rheological properties influence on the electrospinning of caseinate for loading with antioxidant rosemary extract

This study is focused on obtaining bioactive electrospun fibers from aqueous based solutions with sodium caseinate as major component, polyvinyl alcohol (PVA) as minor co-spinning component and rosemary extract as antioxidant principle.The rheological behaviour of precursor solutions was tuned by changing the caseinate concentration, composition of the solvent system, ultrasonication, high pressure treatment or addition of a second polymer in the system. All samples showed non-Newtonian shear-thinning behavior. Electrospinning was performed for concentrated solutions of 15 and 20 wt% caseinate in various water/ethanol/surfactant/polyvinyl alcohol (PVA) formulations, ultrasonicated for short times. Low amounts of 8 wt% PVA related to Cas were enough to convert electrospraying to electrospinning. The aspect of obtained fibers depends on the composition of precursor solutions. Increased amounts of PVA ensure transformation from fibers with large beads to fibers with lateral fringes and spots then to smooth and uniform fibers. Aqueous extract from rosemary was added into precursor solution to impart bioactive properties to the final fibers. This drastically affected the electrospinning behavior, leading to large beads and fringes. Higher amounts of PVA are required to compensate this, but concentration as high as 5 wt% rosemary extracts could be embedded into smooth and uniform fibers. These manifested significant scavenging activity against DPPH radicals and retarded the oxidation of apple pulp, despite the very low amount of the antioxidant bioactive principle. This offers the possibility to use the caseinate electrospun fiber loaded with rosemary extract in applications where antioxidative action are required, such as food packaging.

Antioxidant and Anticancer Activity of Pericarp Water Extracts of Mediterranean Ancient Chestnut Accessions

The residue of chestnut processing generates a large amount of waste material, a resource not adequately exploited. The antioxidant and antitumoral properties of cold and hot water extracts from discarded pericarp of four chestnut Sardinian accessions and one marron variety were studied. The antioxidant capacity of the extracts was determined by spectrophotometric and electrochemical tests. The 1,1-diphenyl-2-pic-rylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) results were highly correlated with each other; likewise, a good correlation was found between Ferric Reducing Antioxidant Power (FRAP) and cyclic voltammetry (CV) values, both based on the direct transfer of electrons. The antiproliferative effect on normal cells (fibroblasts), and on colon (RKO and SW48) and breast (MCF7) cancer cells was evaluated. Additionally, this paper marks the first application of chestnut extracts to investigate their effects on melanoma (B16F10) cells. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test demonstrated that temperature and different extraction times significantly influenced the growth of cells, both normal and tumor. The fibroblast growth was significantly inhibited by moderate doses of cold extracts, while the GI50 values calculated for hot extracts were high, regardless of the accession or cultivar. An even more marked inhibitory action of the cold extracts was observed both on the growth of RKO and SW48 cells and on B16F10 melanoma cells. Otherwise, an extract concentration, both cold and hot, of no less than 243 µg mL−1 is required to achieve a 50% inhibition of MCF7 cell growth.

Antioxidant, phytochemical and fatty acid profile of extracts from bark of Pterocarpus erinaceus

Pterocarpus erinaceus is reported locally to replenish the body nutrient, support the production of red blood cells and possesses anti-inflammatory activity. Standard methods were used to investigate the chemical constituents of the field and market bark samples of P. erinaceus. Field sample of P. erinaceus had significantly higher protein, (14.15%; 13.90%), than the market sample. The bark extracts had iron (4.04mg/100g-5.19mg/100g) and other minerals. Phytochemicals in P. erinaceus bark extracts include; flavonoid, saponin, phytate and tannin. The highest antioxidant activity was obtained from field bark water extract (FBW) with an IC50­­ of 12.53µg/ml. Fatty acid profile of P. erinaceus bark revealed that it contains linoleic acid (72.35%), palmitic acid (18.73%), D-limonene (44.93%) and D-carvone (11.99%). This study gathered that bark extracts of P. erinaceus contain essential nutrients and fatty acid which may support growth in the body and may be employed in the production of functional food.

ULTRASOUND-ENZYME ASSISTED EXTRACTION OF POLYPHENOL AND AMYLASE ENZYME FROM VIETNAMESE RAMIE LEAVES: PREDICTIVE MODELING DEVELOPMENT AND OPTIMIZATION STUDY

Ramie leaves are rich in antioxidant polyphenols and amylase enzyme, which could be a good source for food applications. This study investigated the impact of combined ultrasound-enzyme treatment (U-EAT), including ultrasound intensity (10-30%), content of Viscozyme L. enzyme addition (0.5-1%) and extraction time (5-15 min) on polyphenol recovery, antioxidant activity, and amylase activity. The result revealed that U-EAT could enhance the efficiency of extraction, with a significantly higher yield of bioactive compounds and antioxidant capacity, and amylase enzyme activity compared to an untreated sample. Multiple regression analysis was applied to predict the efficacy of extraction through the values of total phenolic content, antioxidant activity and enzyme activity. The established models shown high accuracy in predicting the effect of the extracted conditions on the yield of the extraction of antioxidant and enzyme amylase. It was found that the maximum yield of extraction could be obtained when the operation conditions were ultrasound intensity of 26.5%, enzyme concentration of 0.87% and extraction time of 10.88 min. Under these optimal conditions, the highest values were found (TPC of 99.047 mgGAE/100 g, antioxidant activity of 65.02% and amylase activity of 45.36 U/mL). In summary, the findings indicate that the utilization of ultrasound combined with the enzymatic extraction process of polyphenol and amylase enzyme from ramie leaves holds potential for enhancing both the extraction yield and bioactivity of the extract. Further study should be concerned with the process of producing food ingredients or products from this extract.

Exploring the Impacts of Sorghum (Sorghum bicolor L. Moench) Germination on the Flour’s Nutritional, Chemical, Bioactive, and Technological Properties

Germination is a natural, simple, and economical process used to improve the quality of nutritional and technological grains. In this study, native and sprouted sorghum flours were characterized regarding their technological properties (particle size distribution, water, and oil absorption capacity, swelling power and solubility, microscopy of starch granules, and pasting and thermal properties). Nutritional and phytochemical characterization profiles, including free sugars, fatty acids, organic acids, tocopherols, and phenolic compounds, were explored through chromatographic methods. The antioxidant, anti-inflammatory, and cytotoxic activities of the respective hydroethanolic extracts were also evaluated. The results showed that the germination process caused significant changes in the flour composition and properties, causing reduced gelatinization temperature and retarded starch retrogradation; an increased content of free sugars and total organic acids; and a decreased content of tocopherols and phenolic compounds. In terms of bioactivity, the sprouted sorghum flour extract showed better lipid-peroxidation-inhibition capacity and none of the extracts revealed hepatotoxicity or nephrotoxicity, which are important results for the validation of the use of the flours for food purposes. Germination is an efficient and alternative method for grain modification that gives improved technological properties without chemical modification or genetic engineering.

Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells

The anti-lung cancer properties of the plant Medicago orbicularis have not been explored yet. Therefore, we identified its phytochemical composition and investigated the antioxidant, anti-hemolytic, and anti-cancerous properties of extracts of this plant in A549 human lung adenocarcinoma cells. The results show that all parts of M. orbicularis (stems, leaves, and fruits) exhibit remarkable hemolytic activities and modest antioxidant capacity. In addition, all extracts showed a dose-dependent anti-cancerous cytotoxic activity against A549 cells, with fruit extracts being the most potent. This cytotoxic effect could be related, at least partly, to the induction of apoptosis, where M. orbicularis fruit extracts reduced the ratio of anti-apoptotic BCL-2/pro-apoptotic BAX, thereby promoting cellular death. Furthermore, the use of M. orbicularis, in combination with a conventional chemotherapeutic agent, cisplatin, was assessed. Indeed, the combination of cisplatin and M. orbicularis fruit extracts was more cytotoxic and induced more aggregation of A549 cells than either treatment alone. GC-MS analysis and total polyphenol and flavonoid content determination indicated that M. orbicularis is rich in compounds that have anti-cancerous effects. We propose M. orbicularis as a potential source of anti-cancerous agents to manage the progression of lung cancer and its resistance to therapy.

Effect of Pleurotus eryngii mycelial fermentation on the composition and antioxidant properties of tartary buckwheat

In this study, we investigated the effect of solid-state fermentation of Pleurotus eryngii on the composition and antioxidant activity of Tartary buckwheat (TB). Firstly, the solid-state fermentation of P. eryngii mycelium with buckwheat was carried out, and the fermentation process was explored. The results of the extraction process and method selection experiments showed that the percolation extraction method was superior to the other two methods. The results of extraction rate, active components and antioxidant activity measurements before and after fermentation of TB extract showed that the extraction rate increased about 1.7 times after fermentation. Total flavonoids, rutin and triterpene contents were increased after fermentation compared to control. Meanwhile, LC-MS results showed an increase in the content of the most important substances in the fermented TB extract and the incorporation of new components, such as oleanolic acid, ursolic acid, amino acids, and D-chiral inositol. The fermented TB extract showed stronger antioxidant activity, while the protein and amino acid contents increased by 1.93-fold and 1.94-fold, respectively. This research was the first to use P. eryngii to ferment TB and prepared a lyophilized powder that could be used directly using vacuum freeze-drying technology. Not only the use of solid-state fermentation technology advantages of edible fungi to achieve value-added buckwheat, but also to broaden the scope of TB applications. This study will provide ideas and directions for the development and application of edible mushroom fermentation technology and TB.

Bioactivity of Myrtus communis from the Montenegro coastline

Although numerous studies have demonstrated the biomedical potential of Myrtus communisL., (Myrtaceae) data on myrtle from Montenegro are scarce. To evaluate antioxidant, antimutagenic and antibacterial activity of myrtle methanolic extracts. Antioxidant activity was evaluated by measuring free radicals scavenging activity, reducing power and enzyme inhibition. The strongest scavenging activity was towards DPPH radical (2,2-diphenyl-1-picrylhydrazyl) (IC501.69-2.25 mg/mL) and superoxide anion (IC500.56 to 0.88 mg/mL), followed by high reducing power (428-472 mgAA/g.DE) and inhibition of XOD (IC500.308-0.6261mg/mL). Antimutagenic activity was evaluated in reverse mutation assays with Escherichia coliWP2 oxyR mutant IC202 and deficient in the induction of antioxidant enzymes. The myrtle extracts strongly inhibited mutagenesis induced by t-BOOH, reaching 70% at the highest concentration applied. Antimicrobial activity was examined on eight different bacterial strains. Gram-positive bacteria, S. epidermis, S. aureusand M. flavus demonstrated the highest sensitivity towards extracts (MICs 4.5-9 mg/mL), but significantly lower towards essential oil (MIC 0.42-3.32 mg/mL).

Enhancing the Antioxidant Potential of Weissella confusa PP29 Probiotic Media through Incorporation of Hibiscus sabdariffa L. Anthocyanin Extract.

Lactic acid bacteria (LAB) produce important metabolites during fermentation processes, such as exopolysaccharides (EPS), which represent powerful natural antioxidants. On the other hand, H. sabdariffa L. anthocyanin extracts protect LAB and support their development. This study uncovers for the first time, the antioxidant profile of Weissella confusa PP29 probiotic media and focuses on elevating its impressive antioxidant attributes by synergistically integrating H. sabdariffa L. anthocyanin extract. The multifaceted potential of this innovative approach is explored and the results are remarkable, allowing us to understand the protective capacity of the fermented product on the intestinal mucosa. The total phenolic content was much lower at the end of the fermentation process compared to the initial amount, confirming their LAB processing. The DPPH radical scavenging and FRAP of the fermented products were higher compared to ascorbic acid and antioxidant extracts, while superoxide anion radical scavenging and lipid peroxidation inhibitory activity were comparable to that of ascorbic acid. The antioxidant properties of the fermented products were correlated with the initial inoculum and anthocyanin concentrations. All these properties were preserved for 6 months, demonstrating the promising efficacy of this enriched medium, underlining its potential as a complex functional food with enhanced health benefits.

Phytochemical Analysis, Antioxidant, Analgesic, Anti-Inflammatory, Hemagglutinin and Hemolytic Activities of Chemically Characterized Extracts from Origanum grosii (L.) and Thymus pallidus (L.)

Origanum grosii (L.) and Thymus pallidus (L.) are medicinal plants recognized for their uses in traditional medicine. In this context, the aim of this article is to highlight the results of a phytochemical analysis (HPLC), with particular emphasis on the antioxidant (DPPH, TAC, and FRAP), analgesic, anti-inflammatory, haemagglutinin-test-related, and hemolytic activities of the total extracts of these plants. Phytochemical analysis via HPLC revealed that licoflavone C (30%) is the main compound in Origanum grosii, while hesperidin (43%) is found in T. pallidus. Evaluation of the antioxidant capacity of Origanum grosii and Thymus pallidus using the DPPH, TAC, and FRAP methods revealed an IC50 of the order of 0.085 mg/mL and 0.146 mg/mL, an EC50 of the order of 0.167 mg/mL and 0.185 mg/mL, and a total antioxidant capacity of between 750 mg EQ/g and 900 mg EQ/g, respectively. Analgesic evaluations revealed writhes inhibition of the order of 97.83% for O. grosii and 90% for T. pallidus. In addition, both plant extracts showed limited hemolytic activity, not exceeding 30% at a concentration of 100 mg/mL. Evaluation of the anti-inflammatory potential showed edema inhibition of the order of 94% (800 mg/kg) for O. grosii and 86% (800 mg/kg) for T. pallidus. These results highlight the potential applications of these extracts in pharmacological research.

The effect of lactic acid fermentation on the phytochemical content of fig leaf extracts compared to single solvent and sequential solvents extraction

The aim of this study was to compare the antioxidant, natural products, and polyphenolic profiles of Ficus carica L. leaf extracts obtained with a sequential solvent extraction (n-hexane, dichloromethane, ethyl acetate, and methanol), a single solvent extraction with one of three different solvents (methanol, ethanol and ethyl acetate), and a fermentation assisted extraction. Extracts were assessed by high-performance thin-layer chromatography (HPTLC) fingerprinting pattern (before and after derivatization with DPPH free radical, anisaldehyde-sulfuric acid reagent and ferric chloride) and with ATR-FTIR phytochemical profiling. The results show that sequential solvents extraction may maximise the extraction of natural products, especially polyphenolics. However, the highest antioxidant activity was achieved with a single solvent extraction with ethyl acetate. Furthermore, fermentation assisted extraction further improves extraction of antioxidants when compared to single extraction from nonfermented material. In conclusion, brining and spontaneous lactic acid fermentation of plant material is a highly desirable method for improving the functional properties of extracts due to its low cost and low energy requirements.

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from Fruit of Sungkai (Peronema canescens)

Peronema canescens, often known as sungkai, is widely used and can be found all around Indonesia. The public believes that the leaves may reduce fever and strengthen the immune system. However, the effectiveness of sungkai fruit has not been thoroughly investigated. In this research, we looked at endophytic fungus extracts from sungkai fruit’s that have antioxidant and antibacterial properties. The study’s results will serve as the foundation for further investigation into the development of potential natural chemicals with antioxidant and antibacterial properties. Morphologically, the endophytic fungi isolated from sungkai fruit were identified. The antioxidant and antibacterial properties of endophytic fungal extracts were studied using the DPPH technique and the paper disk diffusion method. By employing molecular identification and column chromatography to separate the active compounds, the most likely endophytic fungal isolates were found based on the results of the bioactivity tests. Using 1D NMR spectroscopic methods, the chemical’s structure was determined, and the results were compared to NMR data for the same compound published in the literature. Fruit of sungkai had 8 strains of endophytic fungus (RBH1-RBH8). Strong antibacterial and very strong antioxidant activity were shown by the RBH5 isolate (IC50 &lt; 20 μg/mL). Pythium periplocum was determined to be the RBH5 isolate based on molecular testing. Pure chemical compound extracted from RBH5 isolates shown highly potent and potent antibacterial and antioxidant effects. The chemical compound was identified by spectroscopy as 3-hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-γ-butyrolactone. The results of this study serve as the foundation for developing compounds as pharmaceutical raw materials via further research phases.

Chemical properties, antioxidant, and antimicrobial activities of fruit extracts of Crataegus monogyna var. odemisii

Fruit of the Crataegus species (Rosaceae), known as the “Hawthorn” wild plant, has important benefits for human health. The purpose of this study was to determine the chemical properties of one of the local endemic taxa, Crataegus monogyna var. odemisii in west Turkey. Based on the chemical analysis results, antioxidant and antimicrobial activities of fruit extracts, the fruit lipid contents, total phenol, and total flavonoid contents were studied. Five different methods were used to measure antioxidant capacity, namely the CUPRAC, β-Carotene-linoleic acid, DPPH•, ABTS•+, and CUPRAC analyses were used for the measurement of antioxidant capacity. The results showed that the highest antimicrobial activity was against C. albicans, and it was more effective against Gr+ bacteria than Gr- bacteria. The qualitative, as well as quantitative, analyses of lipid contents of the fruits of C. monogyna var. odemisii were made by gas chromatographic analysis. Folin-Ciocalteu reagent was used for determining the total phenolic content, and aluminum chloride was used for determining the flavonoid content. Results revealed that there was a statistically significant relationship between the antioxidant activities and the phenolic and flavonoid contents in the extracts of Hawthorn fruits.

Investigation of antioxidant, enzyme inhibitory effects and total secondary metabolite quantification of Ornithogalum nutans L. methanol

This study was designed to determine the antioxidant and enzyme inhibitory activities of methanol extracts of different parts (aerial and bulb) of Ornithogalum nutans as well as to investigate the total phenolic and flavonoid amounts. While the total phenolic content of the extracts varied between 1.43±0.01/3.05±0.04 mg GAE/g of extract equivalent, the total flavonoid amount varied between 0.33±0.01/1.79±0.02 mg QE/g. Antioxidant activities were determined by DPPH, ABTS, ß-Carotene/Linoleic acid, CUPRAC, and FRAP methods. The antioxidant activity studies determined that the aerial methanol extract exhibited a higher activity than the bulb. Acetylcholinesterase and tyrosinase enzyme inhibitory activities of the extracts were determined, and the extracts showed lower activity than galantamine (89.41±0.05 %) and kojic acid (89.41±0.05 %) used as standard. The results reveal the potential of O. nutans extracts to be used in pharmacological studies in the pharmaceutical and food industries.

Investigation of Phytochemical, Antioxidant and Antidiabetic Potentials of Scabiosa L. (Caprifoliaceae) Species with Chemometric Methods.

In this research, the total phenolic and flavonoid amounts, phenolic compositions, in vitro antioxidant, antibacterial and antidiabetic properties of the methanol extracts obtained from Scabiosa L. (Caprifoliaceae) species distributed in the flora of Türkiye were investigated using chemometric methods. For this purpose, principal component (PCA) and agglomerative hierarchical clustering analysis were performed as chemometric methods. Chlorogenic acid, quinic acid and cyranoside were determined in the extracts. According to chemometric analysis, S. columbaria subsp. ochroleuca var. ochroleuca and S. triniifolia species were found to be valuable in terms of methanol extract yields, total phenolic and flavonoid contents, antioxidant and antidiabetic activities while S. columbaria subsp. ochroleuca var. webbiana species were found to be valuable in terms of phenolic composition. The methanol extracts of Scabiosa species showed high antioxidant activity, with high phenolic and flavonoid contents. Among the tested 13 bacteria, Scabiosa extracts showed only low activity against Klebsiella pneumoniae, Streptococcus pneumoniae and Pseudomonas aeruginosa. The extracts showed high α-amylase and α-glucosidase inhibitory activity. The results show that Scabiosa methanol extracts may be a source of alternative antioxidants that may be beneficial in slowing or preventing the progression of various oxidative stress-related diseases.

Emerging Therapeutic Potential of Polyphenols from Geranium sanguineum L. in Viral Infections, Including SARS-CoV-2.

The existing literature supports the anti-inflammatory, antioxidant, and antiviral capacities of the polyphenol extracts derived from Geranium sanguineum L. These extracts exhibit potential in hindering viral replication by inhibiting enzymes like DNA polymerase and reverse transcriptase. The antiviral properties of G. sanguineum L. seem to complement its immunomodulatory effects, contributing to infection resolution. While preclinical studies on G. sanguineum L. suggest its potential effectiveness against COVID-19, there is still a lack of clinical evidence. Therefore, the polyphenols extracted from this herb warrant further investigation as a potential alternative for preventing and treating COVID-19 infections.

Antioxidant and cytotoxic properties of Combretum paniculatum and Fadogia agrestis extracts on prostate and cervical cancers cell lines

The medicinal plants used in Burkina Faso are essential source of research for bioactive compounds against prostate and cervical cancer, which are major health problems in Burkina Faso and worldwide. This was done by hydro-acetic maceration of the powder of the leafy branches of Combretum paniculatum (C. paniculatum) and Fadogia agrestis (F. agrestis). These extracts were then fractionated using petroleum ether, dichloromethane, ethyl acetate, and n-butanol. A phytochemical screening of these extracts was performed using High-Performance Thin Layer Chromatography (HPTLC). Antioxidant activity was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH∎) and 2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS∎+). The extracts cytotoxicity was assessed by the MTT (Mitochondrial Tetrazolium Test) method on the LNCaP cell lines of prostate cancer and HeLa of cervical cancer. Phytochemical analysis of extracts and fractions revealed the presence of tannins, flavonoids, and steroids in these plants. For antioxidant activity, the ethyl acetate fraction of F. agrestis and the n-butanol fraction of C. paniculatum gave the best inhibition of DPPH∎ with IC50 values of 0.246 ± 0.045 and 0.097 ± 0.008 μg extract/mL, respectively. Using the ABTS∎+ method, the ethyl acetate fractions of C. paniculatum (1.1009 ± 0.0181 μM ET/g) and F. agrestis (0.0316 ± 0.0007 μM ET/g) showed the best activity. In addition, the fractions of dichloromethane (DCM) of F. agrestis (IC50 = 24.69 ± 9.51 μg/ml) and ethyl acetate (AE) of F. agrestis (IC50 = 129.9 ± 6.49 μg/ml) had better activity, respectively on the prostate and cervical cancer LNCaP lines. These properties could justify the use of these plants in traditional medicine.