Antioxidant Enzyme Activities Research Articles

Redox balance and immunity of piglets pre- and post-E. coli challenge after treatment with hemp or fish oil, and vitamin E

This study investigated the influence of polyunsaturated fatty acid composition and vitamin E supplementation on oxidative status and immune responses in weanling piglets pre- and post-E. coli challenge. Suckling piglets (n = 24) were randomly selected from two litters for an oral supplementation (1 mL/day) with fish oil or hemp oil and vitamin E supplementation (60 mg natural vitamin E/mL oil) from day 10 to 28 of age. At day 29 and 30 of age, each piglet was orally inoculated with 6.7 × 108 and 3.96 × 108 CFU of F4 and F18 E. coli, respectively. Blood was sampled from all piglets on day 28 before E. coli challenge and on day 35 of age to investigate immunological and oxidative stress markers in plasma. One week after weaning and exposure to E. coli, a general reduction in the α-tocopherol concentration and activity of GPX1 was obtained. Vitamin E supplementation lowered the extent of lipid peroxidation and improved the antioxidative status and immune responses after E. coli challenge. Hemp oil had the greatest effect on antioxidant enzyme activity. Provision of hemp oil and vitamin E to suckling piglets may reduce the incidence of post-weaning diarrhea.

Dose-dependent reduction of rat colon antioxidant enzyme activities and increased thiobarbituric acid reactive substances (TBARS) by ethidium bromide

The effect of exposure to varying doses of ethidium bromide on the activities of rat colon antioxidant enzymes has been examined. The rats were divided into eight (08) experimental groups of 5 rats each. Group I rats served as the control, not exposed to ethidium bromide (EthBr). Group II rats were administered 5 mg ethidium bromide kg-1body weight (bd wt),Group III 10 mg ethidium bromide kg-1 body weight, Group IV 20 mg ethidium bromide kg-1 body weight, Group V 40 mg ethidium bromide kg-1body weight, Group VI 60 mg ethidium bromide kg-1body weight, Group VII 80 mg ethidium bromide kg-1 body weight, while rats in Group VIII were administered 100 mg ethidium bromide kg-1 body weight. Treatment was done once weekly via gavage for 24 weeks. At the end of the exposure period, each rat was anaesthetized by halothane inhalation. Colon sections were collected, homogenized and the antioxidant enzyme activities in the homogenate supernatants were determined. Relative to the control, colon catalase, superoxide dismutase and glutathione peroxidase showed evidence of significant (p ≤ 0.05) decrease in activity in rats exposed to ≥ 60, ≥ 20 and ≥ 60 mgethidium bromide kg-1body weight respectively. Compared to the control, thiobarbituric acid reactive substance (TBARS), as measured by malondialdehyde (MDA) levels were significantly (p ≤ 0.05) increased in rats exposed to ≥ 20 mg ethidium bromide kg-1 bd wt.There was a strong and significant negative correlation between ethidium bromide dose and colon catalase (r =-0.9823; p˂ 0.001), superoxide dismutase (r = -0.9107; p˂0.001) and glutathione peroxidase (r = -0.9772; p˂ 0.001) activities. There was a strong and positive correlation between ethidium bromide dose and colon MDA levels (r = + 0.9808; p˂ 0.001) but a strong negative correlation between colon catalase (r = - 0.9455; p˂ 0.001), superoxide dismutase (r = - 0.8707; p˂ 0.001) and glutathione peroxidase (r = - 0.9623; p˂ 0.001) and MDA level. Chronic oral exposure of albino rats to ethidium bromide at a dose above the nontoxic threshold of 10 mg kg-1 body weight significantly impaired the activities of colon antioxidant enzymes.

The light-responsive transcription factor SlBBX20 improves saline-alkali resistance of Solanum lycopersicum by affecting photosynthetic capacity, antioxidant capacity, and osmotic adjustment

The transcription factor BBX family is associated with plant responses to abiotic stress. Among the 31 SlBBXs genes in tomato, SlBBX20 is the most strongly induced by saline-alkali stress. However, the response mechanism of the SlBBX20 transcription factor under saline-alkali stress remains unclear. In this study, we investigated the effect of the SlBBX20 transcription factor on saline-alkali tolerance in tomato using wild-type (WT), SlBBX20 overexpression lines, SlBBX20-silenced materials (TRV2-SlBBX20) and their controls (TRV2-empty). Under normal conditions, overexpression of SlBBX20 increased tomato photosynthetic capacity (contents of chlorophyll a, chlorophyll b and carotenoids, net photosynthetic rate), leaf palisade and spongy tissue thickness, and total antioxidant capacity. In contrast, the photosynthetic capacity and total antioxidant capacity of SlBBX20 silenced material did not change significantly relative to the control. Under saline-alkali stress, overexpression of SlBBX20 significantly reduced the relative conductivity, malondialdehyde content, reactive oxygen species levels (O2•−, H2O2), and increased the relative water content, photosynthetic capacity, total antioxidant capacity, antioxidant enzyme activities (peroxidase, superoxide dismutase, and catalase) and proline content in tomato leaves. And silencing SlBBX20 showed the opposite trend. Furthermore, through transcriptome sequencing of WT and SlBBX20-OE lines grown in saline-alkali stress condition at 0 h and 12 h, three differentially expressed genes related to saline-alkali stress (SlP5CR, SlHsp21.6A, SlHsp26.2) were identified. Yeast one-hybrid and dual-luciferase assays confirmed that SlBBX20 might bind to the G-box cis-acting elements in the promoter regions of SlP5CR, SlHsp21.6A, and SlHsp26.2 genes, exerting transcriptional regulatory functions. In conclusion, overexpression of SlBBX20 enhances saline-alkali tolerance in tomatoes by sustaining leaf photosynthetic capacity, reducing ROS accumulation, and elevating antioxidant enzyme activity and proline content.

Combined application of zinc oxide and iron nanoparticles enhanced Red Sails lettuce growth and antioxidants enzymes activities while reducing the chromium uptake by plants grown in a Cr-contaminated soil

Soil contamination with chromium (Cr) is becoming a primary ecological and health concern, specifically in the Kasur and Sialkot regions of Pakistan. The main objective of the current study was to evaluate the impact of foliar application of zinc oxide nanoparticles (ZnO NPs) (0, 25, 50, 100 mg L−1) and Fe NPs (0, 5, 10, 20 mg L−1) in red sails lettuce plants grown in Cr-contaminated soil. Our results showed that both ZnO and Fe NPs improved plant growth, and photosynthetic attributes by minimizing oxidative stress in lettuce plants through the stimulation of antioxidant enzyme activities. At ZnO NPs (100 mgL−1), dry weights of shoots and roots and fresh weights of shoots and roots were improved by 53%, 58%, 34%, and 45%, respectively, as compared to the respective control plants. The Fe NPs treatment (20 mgL−1) increased the dry weight of shoots and the roots and fresh weights of shoots and roots by 53%, 76%, 42%, and 70%, respectively. Application of both NPs reduced the oxidative stress caused by Cr, as evident by the findings of the current study, i.e., at the ZnO NPs (100 mgL−1) and Fe NPs (20 mgL−1), the EL declined by 32% and 44%, respectively, in comparison with respective control plants. Moreover, Fe and ZnO NPs enhanced the Fe and Zn contents in red sails lettuce plants. Application of ZnO NPs at 100 mg L−1 and Fe NPs at 20 mg L−1, improved the Zn and Fe contents in plant leaves by 86%, and 68%, respectively, as compared to the control plants. This showed that the exogenous application of these NPs helped in Zn and Fe fortification in plants. At similar of concenteration ZnO NPs, CAT and APX activities were improved by 52% and 53%, respectively. Similarly, the POD contents were improved by 17% and 45% at 5 and 10 mg/L of Fe NPs. Furthermore, ZnO and Fe NPs limited the Cr uptake by plants, and the concentration of Cr in the leaves of lettuce was under the threshold limit. The exogenous application of ZnO NPs (100 mg L−1) and Fe NPs (20 mg L−1) reduced the Cr uptake in the leaves of red sails lettuce by 57% and 51%, respectively. In conclusion, ZnO and Fe NPs could be used for the improvement of plant growth and biomass as well as nutrient fortification in stressed environments. These findings not only underscore the efficacy of nanoparticle-assisted phytoremediation but also highlight its broader implications for sustainable agriculture and environmental health. However, future studies on other crops with molecular-level investigations are recommended for the validation of the results.

Green-synthesized lignin nanoparticles enhance Zea mays resilience to salt stress by improving antioxidant metabolism and mitigating ultrastructural damage

Soil salinity poses a substantial threat to agricultural productivity, resulting in far-reaching consequences. Green-synthesized lignin nanoparticles (LNPs) have emerged as significant biopolymers which effectively promote sustainable crop production and enhance abiotic stress tolerance. However, the defensive role and underlying mechanisms of LNPs against salt stress in Zea mays remain unexplored. The present study aims to elucidate two aspects: firstly, the synthesis of lignin nanoparticles from alkali lignin, which were characterized using Field Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM), Fourier Infrared Spectroscopy (FT-IR) and Energy Dispersive X-Ray Spectroscopy (EDX). The results confirmed the purity and morphology of LNPs. Secondly, the utilization of LNPs (200 mg/L) in nano priming to alleviate the adverse effects of NaCl (150 mM) on Zea mays seedlings. LNPs significantly reduced the accumulation of Na+ (17/21%) and MDA levels (21/28%) in shoots/roots while increased lignin absorption (30/31%), resulting in improved photosynthetic performance and plant growth. Moreover, LNPs substantially improved plant biomass, antioxidant enzymatic activities and upregulated the expression of salt-tolerant genes (ZmNHX3 (1.52 & 2.81 FC), CBL (2.83 & 3.28 FC), ZmHKT1 (2.09 & 4.87 FC) and MAPK1 (3.50 & 2.39 FC) in both shoot and root tissues. Additionally, SEM and TEM observations of plant tissues confirmed the pivotal role of LNPs in mitigating NaCl-induced stress by reducing damages to guard cells, stomata and ultra-cellular structures. Overall, our findings highlight the efficacy of LNPs as a practical and cost-effective approach to alleviate NaCl-induced stress in Zea mays plants. These results offer a sustainable agri-environmental strategy for mitigating salt toxicity and enhancing crop production in saline environments.

Effects of the Combined Treatment of Trans-2-Hexenal, Ascorbic Acid, and Dimethyl Dicarbonate on the Quality in Fresh-Cut Potatoes (Solanum tuberosum L.) during Storage.

Fresh-cut potatoes (Solanum tuberosum L.) are susceptible to browning and microbial contamination during storage. In this study, the effects of trans-2-hexenal (E2H), ascorbic acid (VC), dimethyl dicarbonate (DMDC), and the combined treatment of E2H, VC, and DMDC on quality deterioration in fresh-cut potatoes were investigated. The response surface methodology (RSM) demonstrated that E2H, VC, and DMDC concentrations of 0.010%, 0.65%, and 240 mg/L, respectively, were the optimum conditions for fresh-cut potato preservation. Further analysis showed that the combined treatment of E2H, VC, and DMDC was the most effective method of reducing quality deterioration in potatoes compared to the control and individual treatments. Furthermore, the combined treatment of E2H, VC, and DMDC could decrease the accumulation of reactive oxygen species (ROS) via improving antioxidant enzyme activities. Meanwhile, energy-metabolism-related enzyme activities and glutamate decarboxylase (GAD) activity were enhanced, while γ-aminobutyric acid transaminase (GABA-T) activity was reduced via the combined treatment of E2H, VC, and DMDC, which contributed to maintaining high energy levels and GABA content in potatoes. These findings suggested that the combined treatment of E2H, VC, and DMDC could protect membrane integrity through enhancing antioxidant capacity, energy levels, and GABA content to maintain quality in fresh-cut potatoes.

Dietary Oryzanol (Ory) Improved the Survival and Growth of Large Yellow Croaker (Larimichthys crocea) Larvae via Promoting Activities of Digestive Enzymes, Antioxidant Capacity, and Lipid Metabolism

Dietary Oryzanol (Ory) Improved the Survival and Growth of Large Yellow Croaker (Larimichthys crocea) Larvae via Promoting Activities of Digestive Enzymes, Antioxidant Capacity, and Lipid Metabolism

GABA attenuates neurotoxicity of zinc oxide nanoparticles due to oxidative stress via DAF-16/FoxO and SKN-1/Nrf2 pathways

Zinc oxide nanoparticles (ZnO-NPs) are one of the most widely used metal oxide nanomaterials. The increased use of ZnO-NPs has exacerbated environmental pollution and raised the risk of neurological disorders in organisms through food chains, and it is urgent to look for detoxification strategies. γ-Aminobutyric acid (GABA) is an inhibitory neurotransmitter that has been shown to have anxiolytic, anti-aging and inhibitory effects on nervous system excitability. However, there are few reports on the prevention and control of the toxicity of nano-metal ions by GABA. In zebrafish, ZnO-NPs exposure led to increased mortality and behavioral abnormalities of larva, which could be moderated by GABA intervention. Similar results were investigated in Caenorhabditis elegans, showing lifespan extension, abnormal locomotor frequency and behavior recovery when worms fed with GABA under ZnO-NPs exposure. Moreover, GABA enhanced antioxidant enzyme activities by upregulating the expression of antioxidant-related genes and thus scavenged excessive O2−. In the case of ZnO-NPs exposure, inhibition of nuclear translocation of DAF-16 and SKN-1 was restored by GABA. Meanwhile, the protective effect of GABA was blocked in daf-16 (−) and skn-1 (−) mutant, suggesting that DAF-16/FoxO and SKN-1/Nrf2 pathways is the key targets of GABA. This study provides a new solution for the application of GABA and mitigation of metal nanoparticle neurotoxicity.

A novel multitrophic biofloc technology for duckweed and Megalobrama amblycephala integrated culture: Improving nutrient utilization and animal welfare

Biofloc technology (BFT) is an eco-friendly aquaculture model that utilizes zero-exchange water. In this study, we investigated the integration of duckweed into BFT in an effort to enhance nitrogen, phosphorus, and carbon utilization and to improve animal welfare for cultivating Megalobrama amblycephala. The experiment spanned 75 days, comparing a group of M. amblycephala supplemented with duckweed (DM) to a control group (CG) with no supplementation, where duckweed consumption relied solely on the feeding behavior of the fish. The concentrations of nitrate, total nitrogen, and phosphorus accumulation were lower in the DM than in the CG from day 45 onwards, with differences of 16.19, 26.90, and 1.45 mg/L, respectively, at the end of the experiment. The DM showed simultaneous increases of 5.77, 11.20, and 5.07 % in the absolute utilization of nitrogen, phosphorus, and carbon, respectively. The abundance of TM7a (10.27 %), linked to nitrate absorption, became the dominant genus in the water of the DM. Additionally, the abundance of Cetobacterium, associated with carbohydrate digestion, was significantly higher in gut of the DM (23.83 %) than in the gut of CG (1.24 %, P < 0.05). Supplementing the diet of M. amblycephala with duckweed improved digestion and antioxidant enzyme activity. Transcriptome data showed that duckweed supplementation resulted in an increase in the expression of genes related to protein digestion and absorption and carbohydrate metabolism in M. amblycephala, and analysis of the significantly enriched pathways further supported improved antioxidant capacity. Based on the above results, we concluded that as M. amblycephala consumes more duckweed, the differences in nitrogen and phosphorus levels between the DM and CG would continue to increase, along with a simultaneous increase in fixed carbon. Thus, this study achieved the goal of recycling BFT resources and improving animal welfare by integrating duckweed.

Promoting effect of biogenic AgNPs on Echinops macrochaetus for improving growth parameters and mitigating the toxic effect caused by salinity stress

Nanoparticles may serve as novel plant growth stimulators to improve plant growth as well as protect plants from abiotic stresses. In this study, biogenic silver nanoparticles (AgNPs) were synthesized using the aqueous extract of Fagonia bruguieri and characterized using UV-visible spectroscopy, Zeta potential, transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). The synthesized AgNPs were employed on Echinops macrochaetus as seeds priming, and plants grown in pots were exposed to salinity stress to assess the ameliorating effects based on various parameters. The growth parameters (shoot length) and biomass (shoot, root and leaves weight) decreased in E. macrochaetus exposed to salinity stress alone (40, 80 and 120 mmol/L NaCl) for 90 days. However, seed priming with a low concentration of AgNPs (40 µmol/L) improved the growth of the plant in terms of biomass, shoot length and chlorophyll content alone as well as ameliorated the toxic effect caused by salinity stress. The mitotic index measured by flowcytometry (FCM) indicated genotoxic effects in plants grown at high concentrations of NaCl (80 and 120 mmol/L) as well as plants obtained with 80 µmol/L AgNPs seed priming and exposed to 120 mmol/L NaCl. Thus, an optimum concentration of AgNPs could be used in E. macrochaetus for production of more biomass by modulating the chlorophyll content, proline and antioxidant enzyme activity.

Ciprofloxacin affects nutrient removal in manganese ore-based constructed wetlands: Adaptive responses of macrophytes and microbes

Ciprofloxacin (CIP) has received considerable attention in recent decades due to its high ecological risk. However, little is known about the potential response of macrophytes and microbes to varying levels of CIP exposure in constructed wetlands. Therefore, lab-scale manganese ore-based tidal flow constructed wetlands (MO-TFCWs) were operated to evaluate the responses of macrophytes and microbes to CIP over the long term. The results indicated that total nitrogen removal improved from 79.93% to 87.06% as CIP rose from 0 to 4 mg L−1. The chlorophyll content and antioxidant enzyme activities in macrophytes were enhanced under CIP exposure, but plant growth was not inhibited. Importantly, CIP exposure caused a marked evolution of the substrate microbial community, with increased microbial diversity, expanded niche breadth and enhanced cooperation among the top 50 genera, compared to the control (no CIP). Co-occurrence network also indicated that microorganisms may be more inclined to co-operate than compete. The abundance of the keystone bacterium (involved in nitrogen transformation) norank_f__A0839 increased from 0.746% to 3.405%. The null model revealed drift processes (83.33%) dominated the community assembly with no CIP and 4 mg L−1 CIP. Functional predictions indicated that microbial carbon metabolism, electron transfer and ATP metabolism activities were enhanced under prolonged CIP exposure, which may contribute to nitrogen removal. This study provides valuable insights that will help achieve stable nitrogen removal from wastewater containing antibiotic in MO-TFCWs.

SiO2 nanoparticle attenuates phytotoxicity of graphene quantum dots in Zea mays (L.) plants

AbstractThe tremendous potential of graphene quantum dots (gqds) in biomedical applications has raised increasing concerns about their risks to ecosystem and human beings. silicon dioxide nanoparticles (sio2 nps) serve as promising nanofertilizers in enhancing plant tolerance against abiotic stresses, but the knowledge of their role in regulating crop responses to gqd stress is far from sufficient in depth and width. The present work offers insight into the effects of sio2 nps on the root uptake and phytotoxicity of gqds in maize (zea mays L.) seedlings. the addition of sio2 nps significantly decreased the accumulation of gqds in the roots and leaves by 33.3% and 58.8%, respectively. physiologically, the presence of sio2 nps led to substantial enhancement in photosynthesis and antioxidant enzyme activities relative to the plants under the gqds stress. responsive differentially expressed genes were mainly associated with crucial pathways regarding photosynthesis, the mapk signaling pathway in the maize plants, and glutathione metabolism. sio2 nps alleviated the gqds‐induced oxidative stress and helped to re‐establish redox homeostasis by up‐regulating the expression of genes related to antioxidant enzyme activities. these results showed that the root application of sio2 nps alleviated the gqd‐induced inhibition to the photosynthesis and growth of crop plants, which are of great significance for advancing the sustainable utilization of nanofertilizers in agriculture.

Cultivar-specific regulation of antioxidant enzyme activity and stomatal closure confer tolerance of wheat to elevated ozone: A two-year open-field study with five cultivars

Elevated concentrations of tropospheric ozone (O3) pose a significant threat to food production in many regions of the world. We conducted a two-year experiment with five winter wheat (Triticum aestivum L.) cultivars, Yangmai16 (Y16), Yangmai23 (Y23), Zhenmai12 (Z12), Yangmai29 (Y29), and Yangmai30 (Y30), to assess photosynthetic traits, lipid peroxidation, and antioxidant systems under ambient (A-O3) or elevated (E-O3; 1.5 times A-O3) O3 in a Free-Air O3Concentration Enrichment system (FACE). E-O3 accelerated leaf senescence, manifested by marked reductions in photosynthetic rate, stomatal conductance, total antioxidant capacity, and photosynthetic pigment content, accompanied by altered antioxidant enzyme activity and a significant increase in malondialdehyde content (an indicator of lipid peroxidation). Most parameters showed significant inter-cultivar differences, as well as interactions between O3 and cultivar. The response of the antioxidant system in wheat flag leaves was influenced by O3 exposure levels. The response of antioxidant enzymes at the grain filling stage and the timely closure of stomata conferred tolerance to O3. This tolerance was associated with higher antioxidant enzyme activity at the anthesis stage but was not related to the size of stomata. This study provides a crucial theoretical foundation for further breeding O3-tolerant cultivars.

Physiological response and molecular mechanism of Quercus variabilis under cadmium stress

Heavy metal pollution is a global environmental problem, and Quercus variabilis has a stronger tolerance to Cd stress than do other species. We aimed to explore the physiological response and molecular mechanisms of Q. variabilis to Cd stress. In this study, the antioxidant enzyme activities of leaves were determined, while the photosynthetic parameters of leaves were measured using Handy PEA, and ion fluxes and DEGs in the roots were investigated using noninvasive microtest technology (NMT) and RNA sequencing techniques, respectively. Cd stress at different concentrations and for different durations affected the uptake patterns of Cd2+ and H+ by Q. variabilis and affected the photosynthetic efficiency of leaves. Moreover, there was a positive relationship between antioxidant enzyme (CAT and POD) activity and Cd concentration. Transcriptome analysis revealed that many genes, including genes related to the cell wall, glutathione metabolism, ion uptake and transport, were significantly upregulated in response to cadmium stress in Q. variabilis roots. WGCNA showed that these DEGs could be divided into eight modules. The turquoise and blue modules exhibited the strongest correlations, and the most significantly enriched pathways were the phytohormone signaling pathway and the phenylpropanoid biosynthesis pathway, respectively. These findings suggest that Q. variabilis can bolster plant tolerance by modulating signal transduction and increasing the synthesis of compounds, such as lignin, under Cd stress. In summary, Q. variabilis can adapt to Cd stress by increasing the activity of antioxidant enzymes, and regulating the fluxes of Cd2+ and H+ ions and the expression of Cd stress-related genes.

Effectiveness of lycopene dietary enrichment for growth performance, digestive enzymes, blood health, immunity, and antioxidant status of common carp (Cyprinus carpio) against chronic glyphosate toxicity

For food safety challenges, sustainable aquaculture emerges as a significant source in recent years; however, despite its potential, the industry still facing challenges, notably the exposure of cultured animals to pesticidal pollution. This pollution originating from agricultural practices that can enter aquaculture system directly: to integrated-agriculture aquaculture practices, or indirectly via soil leakage. Current research based on glyphosate (GLY) toxicity and its amelioration by lycopene (LYC). Four fish groups used for six-weeks experiment in which four groups were used. Control group (CL) was fed with basal commercial diet only without any LYC and GLY exposure; 2) LYC group: exposed to LYC supplemented diet (15 mg/kg per fish diet); 3) GLY group: exposed to glyphosate only (1/5th of 96 h LC50: 0.0892 mg/L) with basal commercial diet, and; 4) GLY + LYC group: exposed to both lycopene supplemented diet (15 mg/kg per fish diet) and glyphosate (1/5th of 96 h LC50: 0.0892 mg/L). GLY observed to decrease growth parameters and feed utilization whereas, lycopene ameliorated growth rate (WG, SGR, HSI, CF) and feeding utilization (FCR) as compared to the control group. Also, GLY induced toxicity within hematobiochemical parameters with alleviation by LYC supplementation. GLY induced cytotoxicity was observed within RBCs as lobbing, notching, vacuolation, blebbing, micronuclei, and condensation. Increase in reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) were observed by GLY exposure. Also, there is observed reduction in antioxidant enzyme activities (CAT, SOD, POD, TPC and GSH) upon GLY exposure. Lipid peroxidation (malondialdehyde: MDA), 8-OHdG (8-hydroxy-2′-deoxyguanosine) and DIY (dityrosine) observed to increase by GLY toxicity. There was improvement in immune responses; increased AChE (acetylcholinesterase) activity, lysozyme content, ACP (acid phosphatase), NBT (nitro blue tetrazolium), NO (nitric oxide) and IgM levels (immunoglobulin M) and digestive enzyme activities (protease, lipase and amylase) observed by LYC supplemented diet. Taken together, LYC supplementation observed to alleviate GLY induced oxidative stress and cytotoxicity with improved immunity, digestive actions and blood health within C. carpio. Therefore, dietary supplementation with lycopene can protect common carp from the harmful effects by glyphosate within agri-integrated aquaculture practices, so suggesting it as potential feed additive.

Streptomycin generates oxidative stress in melanin-producing cells: In vitro study with EPR spectroscopy evidence

Streptomycin (STR) is an aminoglycoside antibiotic with a broad-spectrum of activity and ototoxic potential. The mechanism of STR-induced inner ear damage has not been fully elucidated. It was previously found that STR binds to melanin, which may result in the accumulation of the drug in melanin-containing tissues. Melanin pigment is present in various parts of the inner ear, including the cochlea and vestibular organ.The present study aimed to assess if streptomycin generates oxidative stress and affects melanogenesis in normal human melanocytes. Moreover the variation of free radical concentration in STR-treated melanocytes was examined by electron paramagnetic resonance spectroscopy (EPR). We found that STR decreases cell metabolic activity and reduces melanin content. The observed changes in the activity of antioxidant enzymes activity in HEMn-DPs treated with streptomycin may suggest that the drug affects redox homeostasis in melanocytes. In this work EPR study expanded knowledge about free radicals in interactions of STR and melanin in melanocytes. The results may help elucidate the mechanisms of STR toxicity on pigment cells, including melanin-producing cells in the inner ear. This is important because understanding the mechanism of STR-induced ototoxicity would be helpful in developing new therapeutic strategies to protect patients' hearing.

Silybin prevented avermectin-induced cardiotoxicity in carp by modulating oxidative stress, inflammation, endoplasmic reticulum stress, mitochondrial apoptosis, and autophagy

Avermectin is one of the widely used anthelmintics in aquaculture and exhibits substantial toxicity to aquatic organisms. Silybin is extensively used for its anti-inflammatory, antioxidant and anti-apoptotic biological properties. Heart is essential for the survival of fish and plays a vital role in pumping blood oxygen and nutrients. Residual avermectin in water poses harm to carp. However, there is still insufficient research on whether silybin can mitigate the toxicity of avermectin to carp heart tissues. In this research, we established a model involving carp subjected to acute avermectin exposure and administered diets containing silybin to explore the potential protective effects of silybin against avermectin-induced cardiotoxicity. The results revealed that avermectin induced oxidative stress, inflammation, endoplasmic reticulum (ER) stress, mitochondrial pathway apoptosis and autophagy in the cardiac tissues of carp. Compared with the avermectin group, silybin significantly reduced ROS accumulation in cardiac tissues, restored antioxidant enzyme activity, inhibited mRNA transcript levels of pro-inflammatory-related factors, and attenuated ER stress, mitochondrial pathway apoptosis and autophagy. Protein-protein interaction (PPI) analysis demonstrated that silybin mitigated avermectin-induced cardiac oxidative stress, inflammation, ER stress, mitochondrial pathway apoptosis and autophagy. Silybin exerted anti-inflammatory effects through the Nuclear Factor kappa B (NF-κB) pathway, antioxidant effects through the Nuclear factor erythroid 2-related factor 2 (Nrf2) - Kelch-like ECH-associated protein 1 (Keap1) pathway, alleviated cardiac ER stress through the Glucose-regulated protein 78 (GRP78)/Activating Transcription Factor 6 (ATF6)/C/EBP homologous protein (CHOP) axis, suppressed apoptosis through the mitochondrial pathway, and inhibited excessive autophagy initiation through the PTEN-induced putative kinase 1 (PINK1)/Parkin RBR E3 ubiquitin protein ligase (PARKIN) signaling pathway. This study provided evidence supporting the protective effect of silybin against avermectin-induced cardiotoxicity in carp, highlighting its potential as a dietary additive to protect fish from adverse effects caused by avermectin exposure.

Investigating the effect of parasites (toxoplasma gondii RH strain, Leishmania major (MRHO/IR/75/ER), and hydatid cyst) antigens on Alzheimer's disease: An in vivo evaluation

This study aimed to investigate the effects of parasite antigens on Alzheimer's symptoms in animal model. Alzheimer's model was induced in Wistar rats using Amyloid-beta peptide, and treated with parasite crude antigens from T. gondii RH strain, L. major (MRHO/IR/75/ER), and HC. Spectrophotometry and real-time PCR were used to evaluate the oxidative stress levels, antioxidant enzyme activity, and gene expression of NLRP3, IL-8, IL-1β, and Caspase-1. Histological assays were performed to investigate structural changes in the hippocampus. Apoptosis was analyzed using an Annexin V Apoptosis by Flow cytometer. The levels of total oxidant, antioxidant, and SOD increased in the Alzheimer's group compared with the control group, but these factors were lower in the L. major group. The apoptosis in the treated groups was lower compared to the Alzheimer's group. IL-8 expression was significantly higher in all Alzheimer's groups, but decreased in the HC and L. major treated group compared to Alzheimer's. IL-1β and Caspase-1 expression were similarly increased in all groups compared with the control group, but decreased in the antigen-treated groups compared with Alzheimer's. NLRP3 expression was increased in all groups compared with the control group, with lower expression in HC group, but significantly decreased in L. major group compared with Alzheimer's. In histological results, only L. major group could play a therapeutic role in pathological damage of the hippocampus. The results showed that parasite antigens, specifically L. major antigens, may have neuroprotective effects that reduce oxidative stress, apoptosis, and histopathological changes in response to AD in animal model.

Bergenin attenuates triptolide-caused premature ovarian failure in mice based on the antioxidant activity

Tripterygium wilfordii (TW) preparations have been utilized in China for treating rheumatoid arthritis and autoimmune diseases. However, their clinical use is limited due to reproductive toxicity, notably premature ovarian failure (POF). Our study aimed to investigate the effect and mechanism of bergenin in attenuating POF induced by triptolide in mice. POF was induced in female ICR mice via oral triptolide administration (50 μg/kg) for 60 days. Mice received bergenin (25, 50, 100 mg/kg, i.g.) or estradiol valerate (EV) (0.1 mg/kg, i.g.) daily, 1 h before triptolide treatment. In vitro, ovarian granulosa cells (OGCs) were exposed to triptolide (100 nM) and bergenin (1, 3, 10 μM). Antioxidant enzyme activity, protein expression, apoptosis rate, and reactive oxygen species (ROS) levels were assessed. The results showed that triptolide-treated mice exhibited evident atrophy, along with an increase in atretic follicles. Bergenin (50, 100 mg/kg) and EV (0.1 mg/kg), orally administered, exerted significant anti-POF effect. Bergenin and EV also decreased apoptosis in mouse ovaries. In vitro, bergenin (1, 3, 10 μM) attenuated triptolide-induced OGCs apoptosis by reducing levels of apoptosis-related proteins. Additionally, bergenin reduced oxidative stress through downregulation of antioxidant enzymes activity and overall ROS levels. Moreover, the combined use with Sh-Nrf2 resulted in a reduced protection of bergenin against triptolide-induced apoptosis of OGCs. Together, bergenin counteracts triptolide-caused POF in mice by inhibiting Nrf2-mediated oxidative stress and preventing OGC apoptosis. Combining bergenin with TW preparations may effectively reduce the risk of POF.

Phytic acid-mediated enhancement of Meyerozyma caribbica biocontrol of Aspergillus carbonarius infection in grape berries through regulation of ROS metabolism

Sour rot, an infection caused by Aspergillus carbonarius is one of the detrimental diseases that damage grape vines after harvest. To investigate the biocontrol of this mycotoxigenic fungus, the current study explored the efficiency of Meyerozyma caribbica pretreated with phytic acid (PA) in reducing sour rot in table grapes through the regulation of reactive oxygen species (ROS) production. The content of hydrogen peroxide (H2O2,), superoxide (O2.−) production rate, and the degree of lipid peroxidation were measured. Furthermore, antioxidant enzymes activities, superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and enzymes of the ascorbate–glutathione (AsA-GSH) and glutathione peroxidase (GPX) cycles were investigated. Enzymatic activity results of select genes were confirmed using real-time quantitative polymerase chain reaction (RT-qPCR). Meyerozyma caribbica pretreated with 10 μmol/mL PA (YE) was most efficient in reducing sour decay in grape berries as indicated by an 81.1 % reduction in disease incidence and 1.5 mm lesion diameter by the third day. Phytic acid treated M. caribbica showed a significant reduction in the contents of malondialdehyde (MDA), H2O2, and production rate of O2.− which was complemented by a marked rise in ROS scavenging enzymes’ activities. The H2O2 content in YE was the lowest (24.09 nmol/g FW) by the third day and O2.− production rate was about half that of CK; control (4.19 mmol/min.g FW) on the same day. The SOD, POD, and CAT activities were significantly higher in YE treated fruits (6.63, 18, and 6.64 U/mg FW, respectively) compared to the CK by the fifth day. Subsequently, AsA-GSH and GPX cycles also showed higher activity in grapes inoculated with YE compared to other treatments. Genes encoding SOD, POD, CAT, DHAR, GR, and APX were significantly overexpressed in grapes inoculated with YE compared to the CK, yeast alone, and PA treatments. These findings indicate that PA likely enhances the biocontrol efficacy of M. caribbica, leading to the induction of ROS scavenging mechanisms and subsequent mortality of A. carbonarius in table grapes. Consequently, M. caribbica pre-treated with PA holds promise as a potential biological control agent for A. carbonarius in future applications