Blood Antioxidant Activity Research Articles

Perspectives of treatment of influenza and influenza-like illnesses with Enisamium yodide – Antiviral with anti-inflammatory effect

Background: Enisamium iodide (EI) is a Russian drug whose molecule was developed in the Institute of Pharmacology and Toxicology of Ukraine as an anti-inflammatory and antipyretic agent. Later, other properties of EI were found: inhibition of the influenza virus hemagglutinin, increasing the production of interferon-γ, level of Th1, blood antioxidant activity, inhibition TNF-α and other pro-inflammatory cytokines, activation of macrophages and lysozyme activity. The aim of study was to evaluate the efficacy of antiviral therapy with EI in out-patient patients with influenza and influenza-like illnesses (ILI) without risk factors for severe course of disease. Methods and materials: 124 patients aged 18–55 years with influenza and ILI within 6–48 h of symptoms onset were randomized into 2 groups. 1st group (n = 66) was treated with EI 500 mg tid 5 days, 2nd group (n = 63) received placebo according to the same scheme. In the 1nd group the proportion between influenza and other respiratory viruses was 32.2%: 67.8%, in the 2nd group 31.7%: 68.3%, accordingly. Duration of disease and main clinical symptoms, frequency of complications and speed of the virus elimination from the nasopharynx were estimated. Results: The number of cases with complete recovery within 96 h was 56 (84.8% ± 4.2%) in 1st group and 44 (69.8% ± 5.8%) in 2nd group (p = 0.047). The duration of fever was 68.0 ± 2.8 h in 1th group and 77.1 ± 2.9 h in 2nd group (p = 0.044), muscle pain – 52.3 ± 2.3 and 60.6 ± 2.6 h (p = 0.041), headache-52.1 ± 2.6 and 65.1 ± 2.5 h (p = 0.032), weakness – 75.5 ± 2.3 and 90.4 ± 2.5 h (p = 0.001), accordingly. On the 4th day of treatment the viruses were isolated in 37.8 ± 5.9% of cases in the 1st group and 57.1 ± 6.2% in 2nd group; p = 0.048. Bacterial complications were observed in 3.0 ± 2.1% and 12.6 ± 4.2% accordingly, p = 0.048. Conclusion: The study is demonstrated the effectiveness of EI in the treatment of influenza and ILI in adult outpatient patients. The antiviral and anti-inflammatory effects of the drug, which was administered within 48 h of the symptoms onset were demonstrated by a more rapid reduction of clinical symptoms, frequency of bacterial complications and reduction in the time of virus elimination. Continuation of EI clinical trials are necessary for more full evaluation of its effectiveness.

Prospects of using biotechnology to improve morphofunctional state of liver

The study examines the effect of hepatoprotectors of plant origin, obtained using biotechnology, on the liver morphofunctional state in 465 patients with chronic diffuse diseases. The patients undergo a complex conservative therapy, including hepatoprotectors of plant origin, which helps to correct homeostasis disorders, microcirculatory disorders and blood rheology, increase blood antioxidant activity, and improve the cell membranes structure and function. It is revealed that with intrahepatic cholestasis, regardless of the nosological form, against the background of dysproteinemia, bilirubinemia, azotemia, moderate transaminasemia, we observe a 2.2–4.9 times increase in the of alkaline phosphatase level, moderate cholesterolemia, pronounced phospholipidemia and β-lipoproteinemia. The conducted complex conservative therapy significantly improves the condition of patients and normalizes hemostasis and homeostasis. The inclusion of hepatoprotectors of plant origin in complex conservative therapy is a promising direction in the development of biotechnologies in hepatology.

Characterization of compounds in Cornelian cherry (Cornus mas L.) and its effect on interior milieu in ZDF

The risk of type 2 Diabetes mellitus is constantly increasing and therefore it is necessary to seek effective and new therapies. Cornelian cherry (Cornus mas L.) presents an opportunity in an alternative for treatment of type 2 Diabetes mellitus due its high antioxidant effects. The objective of the study was to assess the effect of Cornelian cherry pulp and stone on ZDF rats, which are suitable biological model for type 2 diabetes. ZDF rats received Cornelian cherry in three doses (1500, 2000 mg kg-1 body weight of the pulp and 250 mg kg-1 body weight of the stone) using the sterile oral rodent gavage for 4 months. Blood glucose, insulin, antioxidant activity and compounds in Cornelian cherry were determined to investigate the effects. Cornelian cherry stone significantly decreased affected blood glucose levels in ZDF rats when compared to the untreated control group. Total polyphenols and phenolic acids had significantly higher values in Cornelian cherry stone against pulp. Also, high concentrations of the anthocyanins were determined in the pulp. The results suggest that Cornelian cherry stone would potentially provide promising source of natural antioxidants and use in the management of Diabetes mellitus.

Effect of dietary supplementation of Lactobacillus acidophilus on blood biochemical profile, antioxidant activity and plasma immunoglobulin level in neonatal Murrah buffalo calves

An experiment was designed to evaluate the effect of dietary supplementation of Lactobacillus acidophilus on blood biochemical profile, antioxidant activity and plasma immunoglobulin level in neonatal Murrah buffalo calves. The 90 day trial was conducted on 24 neonatal Murrah buffalo calves randomly divided into 4 dietary treatments, viz. CON (basal diet alone), T1 (basal diet + L. acidophilus as a fermented milk @ 100 mL/calf/day having 108 CFU/mL), T2 (basal diet + L. acidophilus as a fermented milk @ 200 mL/calf/day having 108 CFU/ml) and T3 (basal diet + L. acidophilus as a fermented milk @ 300 ml/calf/day having 108 CFU/mL). Supplementation of probiotics improved the plasma glucose level in T2 and T3 as compared to CON. Total protein (TP), plasma albumin (A), plasma globulin (G) and A:G ratio did not change with the supplementation of probiotic in calves. Total cholesterol and HDL cholesterol levels in plasma remained same in all the 4 groups. Total antioxidant (TA) activity was higher in T2 and T3 as compared to CON, whereas it was intermediate in T1. Super oxide dismutase (SOD) activity was significantly higher in T1, T2 and T3 groups as compared to CON whereas catalase and glutathione peroxidase (GPx) activity remained same in all groups throughout experimental period. The total plasma immunoglobulin and plasma IgG remained uninfluenced in all the groups. In conclusion, supplementation of L. acidophilus improved energy metabolism and antioxidant capacity in neonatal Murrah buffalo calves.

Can Agro-Industrial By-Products Rich in Polyphenols be Advantageously Used in the Feeding and Nutrition of Dairy Small Ruminants?

Simple SummaryIn the Mediterranean area, where dairy sheep and goats are widespread, the use of by-products in the diet of small ruminants is an ancient practice. Today the great availability of industrial by-products produced at the local level (e.g., grape, olive, tomato and myrtle residues), appears to be a promising strategy for reducing competition with human edible foods and the cost of off-farm produced feeds since they are imported worldwide. Moreover, these co-feeds can contribute to reducing the ecological and water footprint associated with crop cultivation. The presence of bioactive compounds, such as polyphenols, confers added value to these materials. Several positive aspects are apparent when such by-products are included in the diets of small dairy ruminants, in particular on ruminal metabolism, animal health, and the quality of derived products.Recently, the interest in industrial by-products produced at the local level in Mediterranean areas, resulting from fruit and vegetable processes, has increased because of their considerable amounts of bioactive compounds, including polyphenols. In this review, we analyze the most recent scientific results concerning the use of agro-industrial by-products, naturally rich in polyphenols (BPRP), in the diets of small dairy ruminants. Effects on milk production, milk and rumen liquor fatty acid profile, metabolic parameters, and methane production are reviewed. The feed intake and digestibility coefficients were generally depressed by BPRP, even though they were not always reflected in the milk yield. The main observed positive effects of BPRP were on quality of the milk’s FA profile, antioxidant activity in milk and blood, a reduction of rumen ammonia, and, consequently, a reduction of milk and blood urea. The expected beneficial effects of dietary polyphenols in small ruminants were not always observed because of their complex and variable matrices. However, owing to the large quantities of these products available at low prices, the use of BPRB in small ruminant nutrition offers a convenient solution to the valorization of residues arising from agricultural activities, reducing feed costs for farmers and conferring added value to dairy products at the local level, in a sustainable way.

Амперометрическое детектирование водорастворимых антиоксидантов в кормовых добавках на основе хитозана и в сыворотке крови овец

The effects of chitosan on various metabolic and digestive processes in the animal body are an important to study because of the production quality for animal nutrition, healthcare, etc. The major known changes in the biochemical parameters of the animal liquids (by chitosan usage as feed additive) are the following: ratio of volatile and fatty acids, content of fat, mineral composition, etc. The aim of this study was to evaluate the total amount of water-soluble antioxidants (TAWSA) of feed additives based on chitosan and high protein concentrate of microbiological synthesis and to assess the effect of these feed additives on TAWSA values of sheep blood serum by amperometric method. Firstly, the antioxidant activity of feed components based on chitosan or/and a high-protein microbiological synthesis concentrate was studied in this work. Second, the experiments are carried out on 6 fistula animals to confirm the results of laboratory studies. Third, the determination of the total antioxidant activity of sheep blood by using chitosan additives (as the feed components) by amperometric method was carried out. A particular difference in antioxidant activity of the control and experimental samples of the sheep blood was established for the first time.

The influence of harmful physical factors and industrial dust on changes in some biochemical and functional indicators of the cardiovascular system and respiratory system in workers engaged in underground mining of ore

Introduction. The health of workers is determined by both social and individual, as well as production factors, including noise, vibration and dust, characteristic of technological processes in mining enterprises. Industrial noise above 90 dBA and dust in the form of suspended and fine particles causes dysfunction of the cardiovascular system and respiratory organs.The aim of the study is to assess the impact of harmful physical factors and industrial dust on changes in some biochemical and functional indicators of the cardiovascular system and respiratory system of employees of the enterprise for underground mining of ore.Materials and methods. The assessment of working conditions of workers engaged in underground mining and processing of chrome ores, the analysis of industrial dust on the dimension and quantitative content of fine particles PM10 and PM2. 5, the study of biochemical parameters of lipid metabolism, oxidant and antioxidant systems, indicators of respiratory function. Results. Harmful working conditions (class 3.1–3.4) in terms of noise and vibration were revealed at the workplaces of employees of the main specialties observation group. In the air of the working zone, the highest content of fine particles PM2.5 (2.68±0.54 mg/m3) and PM10 (4.64±0.93 mg/m3) was established at the site of drilling operations and cleaning of the bottom-hole space. Deviations of biochemical parameters characterizing intensification of free radical processes and antioxidant protection, imbalance of lipid spectrum parameters, and violation of functional parameters of external respiration were revealed. A high degree of connection with the working conditions of the frequency of increased levels of lipid hydroperoxide and antioxidant activity (EF=60.71–65.84%) was established.Conclusions. In high noise level (more than 94 dBA), general and local vibration (more than 116 and 127 dB respectively) and the content of fine particles PM2. 5 (more of 2.14 mg/m3) and PM10 (over of 3.71 mg/m3) at underground mining of chrome ore workers have identified abnormalities in the form of increase 1.6 times the level of lipid hydroperoxides and total antioxidant activity, reducing to 1.2 times the level of high density lipoprotein, improving 1.2–1.3 times of low-density lipoproteins, triglycerides, and atherogenic index in blood serum, peak expiratory flow. A high degree of professional conditioning have the frequency of increasing the level of lipid hydroperoxide in blood serum and antioxidant activity of blood plasma.

CHEMICAL, TECHNOLOGICAL AND BIOLOGICAL STUDIES ON SOME PLANTS EXTRACTS

The aim of the recent research was to study the chemical, technological and Biological of some plant extracts. Aqueous (hot and cold) water extracts of three plants (Moringa leaves , basil leaves and grape seeds ) were evaluated for their contents of bioactive compounds (phenolic and flavonoids contents) and used individually or as mixture orally (at100mg/kg and 200mg/kg) for treating blood sugar and hypercholesterolemia in rats. No significant difference (p < 0.05) in total phenolic and total flavonoid was detected between cold and hot water extracts of Moringa leaves , basil leaves and grape seeds. The major phenolic compounds in cold water extracts of Moringa leaves was catechien 571.15 ppm and it was cinnamic acid 130.88 ppm in basil leaves meanwhile, grape seeds had Catechein 19.84ppm as the major phenolic fraction as detected by HPLC. On the other side, Hesperidin 4438.53 ppm and1423.06ppm, respectively was the major flavonoid compounds in cold water extracts of Moringa leave and basil leaves Meanwhile,Rutin 6.66 ppm was the abundant in grape seeds. The antioxidant activity (by DPPH) showed no differences (p < 0.05) between cold and hot water extracts of the studied plant material. Catechein was the abundant Phenolic compound of( Mix A) 206.44ppm, (Mix B) 420.09ppm and (Mix A) 158.79ppm and Mixture( B .Gs )57.97ppm cold water extract meanwhile, Cinnamic 186.54ppm was the major phenolic compound detected in Mix D. On the other side , Narinigin was the major Flavonoid compound in ( Mix A) 2165.36ppm, (Mix B) 9201.27 and (Mix C) 2978.84ppm, While Hesperidin was the abundant in (Mix D) 910.17ppm . Mixing cold water extracts of moringa leaves, basil leaves and grape ( Mix A) as well as the mixture of basil leaves and grape Seeds (Mix D). showed the highest improve in blood glucose lipid profile, liver function, kidney function and antioxidant activity marker . The jam formulated from (Mix A) 93.7 flowed by (Mix D) 88.13 showed a higher of sensory properties of functional jam with measured. Mean while the physical analysis of jam formulated from ( Mix C) 183.85 flowed by (Mix B) 182.49. On other hand the highest total phenolic was (Mix B) 0.058 mgGAF-1dw flowed by (Mix A)0.043 mgGAF-1DW. Meanwhile a higher total flavonoid was ( Mix C) 0.013 mgQEg-1dw flowed by (Mix B) 0.009 mgQEg-1dw .

Effect of supplementation of pelleted hazel (Corylus avellana) leaves on blood antioxidant activity, cellular immune response, and heart beat parameters in sheep1.

Hazel leaves (Corylus avellana) fed to sheep resulted in decreased methane emissions without negatively affecting feed intake and were found to have antioxidant properties in vitro. The objective of this study was to evaluate effects of hazel leaves, rich in tannins, on blood antioxidant activity, cellular immune response, and heart beat parameters in sheep. Four experimental pellets were produced by mixing alfalfa and hazel leaves in different proportions, including alfalfa alone as a control, 30% and 60% of hazel leaves, the latter also with 3.8% polyethylene glycol (PEG). Six adult, nonpregnant, nonlactating female sheep (71 ± 5.7 kg of body weight) were allocated to 4 treatments in a 6 × 4 crossover design with four 18-d periods. The diet consisted of experimental pellets and ryegrass-dominated hay (ratio 80% to 20% in dry matter), resulting in hazel leaf proportions of approximately 0%, 25%, and 50% in the total diet. Blood samples were collected at the end of each period to determine plasma total phenol concentration and markers of oxidative status as well as peripheral blood mononuclear cells (PBMC) activation and proliferation response in vitro. Heart rate (HR) and HR variability parameters were measured for 2 consecutive days in each period, during different activities (i.e., eating pellets or hay, or lying). Treatments were compared with multiple comparisons and contrast analysis was used to test for linear and quadratic relations. Compared with control, feeding a high dosage of hazel leaves enhanced (P = 0.006) the plasma total antioxidant capacity, which linearly (P = 0.016) increased with increasing level of hazel leaves in the diet. The total phenol concentration and activities of the antioxidant enzymes superoxide dismutase, catalase, and glutathione reductase in the plasma were not different (P ≥ 0.23) among the treatments; however, the latter slightly increased linearly (P = 0.047) with increasing hazel leaves proportion. No differences were observed in the activation and proliferation of PBMC among treatments. The HR decreased linearly (P ≤ 0.009) during pellet eating and lying and the root mean square of successive differences of interbeat intervals (RMSSD) increased linearly (P = 0.037) when lying with increasing level of hazel leaves in the diet. In conclusion, our findings indicate that hazel leaves are a promising supplement to improve oxidative status with no effect on cellular immune response and cardiac stress level of sheep.

Performance, Biochemical Profile and Antioxidant Activity of Hens Supplemented with Addition of Milk Thistle (Silybum marianum) Seed Cakes in Diet

The aim of this study was to evaluate the effect of milk thistle seed cakes addition in laying hens diet to performance, blood biochemical parameters and antioxidant activity. A total of 30 Bovans Brown hens were included to the experiment. The trial was performed from the age of 69 weeks to 80 weeks of hens age. The experimental group received feed mixture containing 7% milk thistle seed cakes. Control group received feed mixture without milk thistle seed cakes. After the 69th week of age, the laying hens in the experimental group reached a higher number of eggs and produced more egg mass compared to the control group. In the evaluation of the egg quality parameters, higher Haugh units, a higher millimeter height of the egg and thinner eggshell in the group receiving 7% of the seed cakes were found. When evaluating health indicators, higher antioxidant activity was found in the experimental group. Blood biochemical parameters was without any differences.

Cover Image, Volume 43, Issue 6

The cover image is based on the Full Article In vitro simulated digestion and in vivo metabolism of chlorogenic acid dimer from Gynura procumbens (Lour.) Merr.: Enhanced antioxidant activity and different metabolites of blood and urine by Jingfang Li et al., DOI 10.1111/jfbc.12654.

Effect of dietary vanadium supplementation on growth performance, mineral balance and antioxidant activity in male Sahiwal calves

The study was aimed to examine the effect of supplementation of sodium metavandate (NaVO3) as source of vanadium on DMI intake, growth performance, antioxidant activity, level of mineral in plasma and their balance in male Sahiwal calves. The vanadium content in maize (Zea mays) and bajra (Pennisetum glaucum) grains was 58 ppb and 55 ppb while in berseem (Trifolium alexandrinum) and mustard (Brassica campestris) fodder it was 8.37 and 7.24 ppm, respectively. Male Sahiwal calves (20) of comparable age (6±0.82 months) and body weight (71±8.06 kg) were randomly allotted to 4 different treatments with replication of 5 animals in each. Supplementation was done with 0, 2, 4 and 8 ppm of vanadium in groups T1, T2, T3 and T4, respectively, for 120 days. Blood samples were collected at monthly intervals to examine antioxidant activity in blood, plasma and mineral levels. Feed consumption (DM intake, DM intake% BW) and growth rate did not show any significant effect of vanadium supplementation. Glutathione peroxidase activity was higher in groups T3 and T4 as compared to T1 and T2 whereas, SOD and catalase activity was similar in all the groups. Excretion and absorption patterns of Ca, P, Cu and Fe and their plasma levels were similar in different groups. However, vanadium and Zn balance and their plasma levels increased due to vanadium supplementation. The present study revealed that in growing calves, vanadium supplementation showed enhanced glutathione peroxidise activity, plasma Zn and vanadium levels.

Red Grape Pomace Rich in Polyphenols Diet Increases the Antioxidant Status in Key Organs-Kidneys, Liver, and Spleen of Piglets.

Simple SummaryGrape pomace (GP) is a residue generated by the wine industry that is produced in large quantities. This work aims to describe the beneficial effects replacing 5% of the corn in the diet of piglets with grape pomace. GP, besides other components, contains some important bioactive compounds called polyphenols. The study shows that the polyphenols from a 5% grape pomace diet are absorbed into the blood of piglets that receive it. These bioactive molecules have a beneficial action on the health status of the animals and also increase the antioxidant activity in the liver, spleen, and kidneys, which are key organs in the metabolism of the nutrients. The aim of this work was to evaluate the influence of a diet containing 5% dried GP on the antioxidant status (total antioxidant status (TAS), antioxidant enzyme activity (catalase-CAT, superoxide dismutase-SOD, and gluthatione peroxidase-GPx), and lipid peroxidation) on the key organs of the liver, kidneys, and spleen in relation to health status as indicated by blood biochemical parameters and total polyphenol content in the blood, organs (liver, spleen, kidney, mesenteric lymph nodes, heart, and brain) and Longissimus dorsi muscle in piglets. The GP diet results in a significant increase of TAS in the liver, spleen, and kidneys, with increased CAT activity in the spleen and kidneys, increased SOD activity in the liver, kidneys, and spleen, and increased GPx activity in the kidneys, as well as a decrease in lipid peroxidation in the liver and kidneys. The GP included in the piglets’ feed contained polyphenols that showed antioxidant activity and were absorbed in the plasma, contributing to maintaining the good health of the animals. The inclusion of 5% GP inclusion in the diets of piglets is beneficial for overall normal blood constituent metabolism and helps to maintain piglet health by increasing the polyphenol content in blood plasma and antioxidant activity in the liver, spleen, and kidneys.

Melatonin Aggravated Oxaliplatin-Mimicking Sinusoidal Obstruction Syndrome: Role of Platelet Aggregation and Oxidative Stress

(1) Background: Sinusoidal obstruction syndrome is a liver injury induced either by accidental ingestion of pyrrolizidine alkaloid monocrotaline or by chemotherapeutic drug oxaliplatin used for the treatment of colorectal cancer. Sinusoidal obstruction syndrome is characterized by rounding and swelling of the sinusoidal endothelial cell, which leads to obstruction of blood vessels, leukocyte infiltration and oxidative stress. Melatonin, a powerful antioxidant, prevents acute liver injury. We investigated the effect of melatonin on monocrotaline-induced sinusoidal obstruction syndrome in mice. (2) Methods: Male C57BL/6JNarl mice were injected with a single dose (500 mg/kg) of monocrotaline to induce sinusoidal obstruction syndrome. Melatonin (1-30 mg/kg) was injected 1 h before monocrotaline treatment. After 24 h of monocrotaline treatment, mice were sacrificed. Hepatic collagen, oxidative stress, antioxidant activity, and the expression of apoptosis protein were measured. (3) Results: Melatonin aggravated monocrotaline-induced sinusoidal obstruction syndrome. In addition, it not only increased serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, neutrophil count, histological score, hepatic platelet aggregation, oxidative stress, and activated apoptosis signaling pathway, but also decreased red blood cell count, hematocrit ratio, platelet count, lymphocyte count, hepatic collagen, and antioxidant activity. (4) Conclusions: To conclude, melatonin aggravated monocrotaline-induced sinusoidal obstruction syndrome via hepatic platelet aggregation and oxidative stress, ultimately leading to activation of apoptosis.

Dietary supplementation of 11 different plant extracts on the antioxidant capacity of blood and selected tissues in lightweight lambs.

Due to the growing public concern regarding the addition of chemical antioxidants to foods, focus has shifted towards natural alternatives. Because of their antioxidant potential, culinary herbs and spices have long been used to extend the shelf-life of foods. However, a better understanding of the fate of these products following intake is required to assess their use in lamb diets. Two hundred and eighty-eight Rasa Aragonesa male lambs (70 days old) were supplemented (5.0 g kg-1 compound feed) with bay, marjoram, oregano, rosemary, thyme, turmeric, cumin, caraway, dill, cinnamon and nutmeg extracts for 14 days before slaughter. Dietary supplementation with plant extracts had no effect on intake, growth performance or antioxidant activity in blood (TEAC values). In muscle, nutmeg supplementation increased (P < 0.05) the radical-scavenging capacity (TEAC), whereas a decrease in the radical-scavenging capacity was found for lambs supplemented with oregano, dill, cinnamon and nutmeg (ORAC values). In liver, nutmeg supplementation increased (P < 0.05) the antioxidant capacity (TEAC), whereas bay (ORAC), turmeric, cinnamon and nutmeg (DPPH• values) decreased (P < 0.05) the radical-scavenging capacity of the tissue. In kidney, a lower (P < 0.05) radical-scavenging capacity (TEAC values) was found in lambs supplemented with oregano, cumin and caraway, whereas, turmeric, cumin, caraway, cinnamon and nutmeg increased (P < 0.05) the antioxidant capacity (ORAC values) in kidney. Supplementation of lamb diets with plant extracts affected radical-scavenging activity in muscle, liver and kidney. However, due to the divergent results of the different assays for the same tissue, it is not advisable to discriminate plant extracts using this approach. © 2019 Society of Chemical Industry.

INFLUENCE OF LASER TREATMENT ON INDICATORS OF FREE-RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN SECONDARY PYELONEPHRITIS

Relevance. Optimization of the conservative tactics of acute inflammation of the kidney in nephrolithiasis makes it advisable to use non-traditional methods of detoxification low-intensity laser radiation (LILR) in particular. Aim. To evaluate the effectiveness of laser therapy (LT) in the complex correction of acute secondary pyelonephritis (ASP) in patients with single kidney stones according to the basic adaptation criteria of oxidative-antioxidant clinical medicine КЛИНИЧЕСКАЯ МЕДИЦИНА homeostasis of the organism. Materials and methods. Indices of endogenous intoxication (EI), characterizing the oxidative-antioxidant homeostasis of the body in ASP, were studied in 96 patients with nephrolithiasis aged 19 to 40 years. Patients of the comparison group (48 people) underwent basic (according to the standards) treatment aimed at controlling of calculous pyelonephritis. Patients of the main group (48 people) in the traditional conservative therapy from the second day of hospitalization daily received additional LT sessions with apparatus LTA “Uzor-3KS” with a frequency of 0.6 kHz, a power of 1.5 watts within 4 minutes (total for treatment course is eight procedures). The effectiveness of therapy was assessed on the 5th and 9th day of the inpatient stay. Results. It was found that endotoxicosis in non-destructive forms of ASP is caused by the increase in the concentration of malonic dialdehyde of plasma (by 20.3%) and erythrocytes (by 35.5%), decrease in catalase activity (by 14.2%) and the amount of total (by 11.8%) and free (21.1%) thiol groups with a decrease in the detoxification index (DI) by 24,1%. Compared with the baseline therapy (BT), the effectiveness of LT in the complex treatment of ASP in the analyzed parameters of metabolic homeostasis against the background of the increase in catalase capacity from 6.9 to 10.2%, the detoxification index from 5 to 12.5% was 7.5% (on the 5th day) and 17.5% (on the 9th day of treatment). Conclusions. ASP leads to activation of ROS, suppression of antioxidant and catalase activity of blood, reduction of DI, indicating a subcompensated syndrome of EI. Standard therapy contributes to the normalization of ROS and SLA values of body protection and reduces the level of endotoxicosis caused by ASP. Additional inclusion in the complex treatment of LT sessions more significantly normalizes redox metabolic homeostasis and EI parameters, improving the effectiveness of basic therapy.

Pharmacological Signatures of the Exenatide Nanoparticles Against Hepatic Ischemia/Reperfusion-induced Pancreatic Injury

BackgroundHepatic ischemia/reperfusion-induced pancreatic injury (HI/RPI) is an important pathophysiological phenomenon in clinics. Exenatide is found to have hepatopancreatic protection; however, the half-life of exenatide is extremely short, which limits its clinical application. In the present study, we described an exenatide nanocarrier based on poly(L-lysine)-poly(ethylene glycol)-poly(L-lysine) (PLL-PEG-PLL) and aimed to investigate the protective effects of exenatide/PLL-PEG-PLL on HI/RPI. MethodsPLL-PEG-PLL was synthesized and estimated by being applied as a nanocarrier for lengthening delivery of exenatide. Exenatide was loaded into PLL-PEG-PLL by electrostatic interactions at pH 7.4. The loading and release of exenatide from PLL-PEG-PLL were characterized in vitro. The pancreatic protection of exenatide/PLL-PEG-PLL was assessed using the animal model, histopathological examination, blood biochemical indices detection, antioxidant activity, and anti-inflammatory evaluation in vivo. ResultsExenatide/PLL-PEG-PLL displayed efficient loading and sustained release. Exenatide/PLL-PEG-PLL complex moderated HI/RPI and enlarged islet functionality compared to free exenatide. ConclusionWe propose that the nanocarrier PLL-PEG-PLL may function as a potent exenatide nanocarrier for augmenting anti-HI/RPI pharmacotherapy with unprecedented clinical benefits.

Pro- and Antioxidant Activity in the Blood of Adolescents of Different Ethnic Groups

Background. Processes of antioxidant protection in different ethnicities, living in the same geographical conditions, are of undoubted scientific interest. The ratio of activity of oxidative processes and antioxidant components not only reflects, but also largely determines the metabolism and adaptive capabilities of the organism. The key issue in understanding the regulatory and adaptive role of lipid peroxidation reactions in the body is the idea of the existence of a certain balance with the antioxidant defense system.Aims: to study the features of the system of lipid peroxidation and antioxidant protection in adolescents of different ethnicities, living in Eastern Siberia.Materials and methods. Parameters of lipid peroxidation and antioxidant protection were determined in the blood serum by spectrophotometric methods.Results. We found that adolescent Buryat boys had statistically significant decrease in primary and secondary products of lipid peroxidation: ketodienes and conjugated trienes, malondialdehyde, and components of antioxidant protection: retinol, α-tocopherol, the level of total antioxidant activity of blood and reduced glutathione, in comparison with Caucasian adolescents. In Caucasian adolescents with endocrine disorders, there was a statistically significant increase in the level of ketodienes and conjugated trienes, as well as the activity of superoxide dismutase against the background of a decrease of reduced glutathione, tocopherol, and retinol. We revealed a significant increase in the concentration of ketodienes and conjugated trienes and a decrease of the oxidized glutathione in adolescents with endocrine disorders in comparison with healthy adolescents of Buryat ethnicity.Conclusions. Studying of the processes of lipid peroxidation and antioxidant system of the organism can be used as an additional criterion for complex examination of practically healthy adolescents, which significantly enhance the representation about the adaptation possibilities of the organism to external conditions and can become a basis for effective examination of reproductive health in the future.

Isolated Obesity Is Not Enough to Impair Cardiac Autonomic Modulation in Metabolically Healthy Men

ABSTRACTPurpose: To evaluate whether excess body mass influences the heart rate variability (HRV) indexes at rest, and to correlate adiposity indicators and the aerobic fitness with cardiac autonomic variables in metabolically healthy young adults. Method: In all, 41 untrained males (Mage = 21.80, SD = 2.14 years), 14 normal weight (MBMI = 22.28, SD = 1.86 kg∙m−2), 11 overweight (MBMI = 26.95, SD = 1.43 kg∙m−2), and 16 obese (MBMI = 33.58, SD = 3.06 kg∙m−2) metabolically healthy (normal values of blood pressure, fasting blood glucose, triglycerides, and total cholesterol), underwent evaluations of the HRV at rest and of the peak oxygen consumption (VO2 peak) during maximal exercise on a cycle ergometer. Results: Blood pressure, heart rate, HRV indexes, casual blood glucose, oxidative stress, and antioxidant activity did not differ among the groups. The VO2 peak (mL∙kg−1∙min−1) was lower in the obese group compared with the normal weight and overweight groups. The body mass (r = −.40 to −.45) and abdominal circumference (r = −.39 to −.52) were slightly to moderately correlated with SD1, SD2, RMSSD, SDNN, pNN50, LF, and HF indexes and total power. The VO2 peak (mL∙kg−1∙min−1) was slightly to moderately correlated (r = .48 to .51) with SD2, SDNN, and LF indexes in the individuals with excess body mass. Conclusion: Cardiac autonomic modulation at rest was preserved in metabolically healthy obese young men. However, the indicators of adiposity, as well as the aerobic fitness were correlated with cardiac autonomic modulation in the individuals with excess body mass.

Impact of space allowance on performance traits, brain neurotransmitters and blood antioxidant activity of New Zealand White rabbits

The objective of this trial was to investigate the effect of space allowance on performance, welfare-related parameters and the levels of brain neurotransmitters in growing male rabbits. In a cage housing system, a total of 96 weaned rabbits were accommodated on three space allowance conditions (S1 = 1425 cm2/rabbit; S2 = 850 cm2/rabbit; S3 = 625 cm2/rabbit), with 8 replicate cages per each group. Rabbits in the S1 and S2 groups showed better daily feed intake and feed conversion ratio compared with the high stocking density group (p = 0.004 and 0.018, respectively). Compared to the highest stocking density group (S3), rabbits in the S1 and S2 groups showed significantly lower serum cortisol, MDA and GSSH level (p = 0.026, 0.018 and 0.009, respectively). The concentration of dopamine in brain tissues was significantly decreased in the S3 group compared with other experimental groups (P = 0.001). However, there was no significant effect of space allowance on the brain AChE level (P = 0.277). Brain serotonin and GABA levels showed a significant decrease in rabbits reared with a limited space allowance (S3) compared with S1 and S2 groups (P = 0.001 and 0.038, respectively). The level of brain MDA was significantly increased in the S3 group compared with the S1 group (P = 0.006). However, there were no detectable differences in the brain ATP level in rabbits reared with different space allowance (P = 0.693). In conclusion, the current study indicates that the 850 cm2/rabbit stocking density has resulted in a better feed intake, and welfare-related conditions compared with the 625 cm2/rabbit. Furthermore, the limited space allowance may impair the most important brain neurotransmitters in male rabbits.