Antioxidant Activity Research Articles

Chemical Composition of Extracts from Various Parts of Feverfew (Tanacetum parthenium L.) and Their Antioxidant, Protective, and Antimicrobial Activities

Tanacetum parthenium is a medicinal plant from the Asteraceae family that can be applied externally in the case of various skin diseases. The aim of the study was to perform a phytochemical analysis of hydroethanolic extracts from the aerial parts (herb), flower heads, and leaves of feverfew and to assess their biological properties. Hydrodistilled oils were analyzed using GC-MS. The chemical composition of the extracts was estimated using spectrophotometry and the HPLC method. Moreover, the extracts were evaluated to determine their antioxidant potential using DPPH and FRAP and measuring the intracellular level of ROS. The cytotoxicity of extracts toward keratinocytes and fibroblasts was also analyzed, as well as their antimicrobial properties against 12 microorganisms. The results of the research revealed that chrysanthenone and α-thujone were the dominant volatile compounds in the essential oil from the flowers, while camphor, trans-chrysanthenyl acetate, and camphene were predominant in the essential oil from the leaves and herb. The results of HPLC showed that the major polyphenol compounds present in the hydroethanolic extracts from various parts of T. parthenium were 3,5-dicaffeoyl-quinic acid, chlorogenic acid, and 3,4-dicaffeoyl-quinic acid. The extract from feverfew flowers was shown to have the highest content of total polyphenols, flavonoids, and phenolic acids, as well as the highest antioxidant potential. In turn, the herb extract had the highest content of condensed tannins and terpenoids and exhibited the most effective antimicrobial properties against the 12 bacterial and fungal strains. Moreover, the hydroethanolic extracts from different parts of T. parthenium plants were shown to have a potent protective effect on skin cells. The present study supports the potential applications of Tanacetum parthenium in the cosmetic and pharmaceutical industries.

Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway

Objectives: Isostrictiniin (ITN), a natural polyphenol extracted from Nymphaea candida (snow-white waterlily), has antioxidant and hepatoprotective activities that may be beneficial in treating metabolic dysfunction-associated steatotic liver disease (MASLD). This study aimed to investigate the protective effects of ITN on high-fat, high-sugar diet (HFSD)-induced steatosis in MASLD mice and its mechanisms. Methods: Kunming mice were randomly divided into normal control and HFSD groups. After being fed for 4 weeks, the HFSD group was randomly divided into model, atorvastatin calcium (ATC; 10 mg/kg), and ITN (25, 50, and 100 mg/kg) groups. After continued feeding for 4 weeks, the biochemical indexes in the mice were determined. Results: Compared with the model group, the liver index; FBG; HOMA-IR; serum AST, ALT, TG, TC, and LDL-C; and liver MDA, IL-6, TNF-α, and IL-1β levels in the ITN (25, 50, and 100 mg/kg) and ATC (10 mg/kg) groups were significantly decreased (p < 0.05), while serum HDL-C and liver SOD and GSH-Px levels were increased (p < 0.05). Pathological observation showed that ITN treatment mitigated the lipid liver deposition in the HFSD mice. Moreover, ITN could upregulate liver-tissue p-AMPK/AMPK protein expression in the HFSD-induced MASLD mice and downregulate SREBP-1c and ACC levels (p < 0.05). Conclusions: ITN can significantly improve MASLD mice, and its mechanism may be related to the regulation of the AMPK/SREBP-1c/ACC pathway.

Potential Vitamin E Signaling Mediators in Skeletal Muscle

Vitamin E (Vit E) deficiency studies underline the relevance of this vitamin in skeletal muscle (SkM) homeostasis. The knowledge of the effectors and modulators of Vit E action in SkM cells is limited, especially in aging and chronic diseases characterized by a decline in musculoskeletal health. Vit E comprises eight fat-soluble compounds grouped into tocopherols and tocotrienols, which share the basic chemical structure but show different biological properties and potentials to prevent diseases. Vit E has antioxidant and non-antioxidant activities and both favorable and adverse effects depending on the specific conditions and tissues. In this review, we focus on the actual knowledge of Vit E forms in SkM functions and new potential signaling effectors (i.e., bioactive sphingolipids and myokines). The possible advantages of Vit E supplementation in counteracting SkM dysfunctions in sarcopenia and under microgravity will also be discussed.

Perilla Seed Oil: A Review of Health Effects, Encapsulation Strategies and Applications in Food

Perilla (Perilla frutescens L.) is an annual herbaceous plant whose seed oil is rich in unsaturated fatty acids such as alpha-linolenic acid (ALA). This oil exhibits various health benefits, including antioxidant, anti-inflammatory, lipid-lowering, hypoglycemic, neuroprotective and immunomodulatory activities. In addition, incorporating perilla oil into a diet can effectively increase the abundance of beneficial bacteria in the gut microbiota. However, perilla oil is prone to oxidation, which reduces its nutritional value and lowers its bioavailability. To address these issues, encapsulation technologies such as emulsions, oleogels, liposomes and microcapsules have been employed, showing promising results. Nonetheless, further research is needed to fully elucidate the underlying mechanisms of perilla seed oil’s health effects, validate its benefits through large-scale human clinical trials and optimize encapsulation techniques. Future investigations should also explore the synergistic effects of combining perilla seed oil with other functional components and its role in modulating gut microbiota to achieve comprehensive health benefits.

Mitigating Methylglyoxal-Induced Glycation Stress: The Protective Role of Iron, Copper, and Manganese Coordination Compounds in Saccharomyces cerevisiae.

Glycation-induced stress (G-iS) is a physiological phenomenon that leads to the formation of advanced glycation end-products (AGEs), triggering detrimental effects such as oxidative stress, inflammation, and damage to intracellular structures, tissues, and organs. This process is particularly relevant because it has been associated with various human pathologies, including cancer, neurodegenerative diseases, and diabetes. As therapeutic alternatives, coordination compounds with antioxidant activity show promising potential due to their versatility in attenuating oxidative stress and inflammation. Herein, we investigated the antioxidant-related protective potential of a series of complexes: [Cu(II)(BMPA)Cl2] (1), [Fe(III)(BMPA)Cl3] (2), and [Cl(BMPA)MnII-(μ-Cl)2-MnII(BMPA)-(μ-Cl)- MnII(BMPA)(Cl)2]•5H2O (3), all synthesized with the ligand bis-(2-pyridylmethyl)amine (BMPA) in Saccharomyces cerevisiae exposed to G-iS caused by methylglyoxal (MG). Pre- treatment with complexes 1 - 3 proved highly effective, increasing yeast tolerance to G-iS and attenuating mitochondrial dysfunction. This observed phenotype appears to result from a reduction in intracellular oxidation, lipid peroxidation levels, and glycation. Additionally, an increase in the activity of the antioxidant enzymes superoxide dismutase and catalase was observed following treatment with complexes 1 - 3. Notably, although complexes 1 - 3 provided significant protection against oxidative stress induced by H2O2 and menadione, their protective role was more effective against MG-induced glycation stress. Our results indicate that these complexes possess both antiglycation and antioxidant properties, warranting further investigation as potential interventions for mitigating glycation and oxidative stress-related pathologies.

Oat-Protein-Based Diet Lowers Blood Pressure and Prevents Cardiac Remodeling and Dysfunction in Spontaneously Hypertensive Rats

Background/Objectives: Hypertension and its associated complications, such as cardiac remodeling and dysfunction, continue to impose a significant burden on global healthcare. Nutritional interventions have been recognized as playing a crucial role in addressing this devastating condition termed a ‘silent killer’. Plant-based proteins could potentially be utilized as a non-pharmacological strategy to combat hypertension and its related risk factors. In this study, we investigated the efficacy of an oat protein diet in managing hypertension and cardiac abnormalities. Methods: Four-week-old male spontaneously hypertensive rats (SHRs) and Wistar–Kyoto (WKY) rats were fed a regular diet with casein as a protein source or an oat-protein-based diet for 16 weeks. Twenty-week-old male SHRs showed high blood pressure (BP), cardiac remodeling, cardiac dysfunction, higher levels of markers of oxidative stress [malondialdehyde (MDA)] and inflammation [tumor necrosis factor-α (TNF-α)], as well as lower levels of a marker of vascular function (nitric oxide). Results: The oat protein diet was able to significantly lower high BP, prevent cardiac remodeling and dysfunction, improve the levels of nitric oxide, and reduce the levels of TNF-α. Oat protein, after in vitro gastrointestinal digestion, also exhibited angiotensin-converting enzyme inhibition and significantly higher antioxidant activity than casein when assessed with the 2,2-diphenyl-1-picrylhydrazyl and the iron-chelating assays in vitro. Conclusions: oat protein lowers BP and prevents cardiac remodeling and dysfunction partly via improving the levels of nitric oxide and TNF-αin SHRs. Its high antioxidant potential could contribute to the observed cardiovascular effects.

Drought stress mitigation and improved yield in Glycine max through foliar application of zinc oxide nanoparticles.

The impact of climate change on agricultural production is apparent due to declining irrigation water availability vis-à-vis rising drought stress, particularly affecting summer crops. Growing evidence suggests that zinc (Zn) supplementation may serve as a potential drought stress management strategy in agriculture. Field studies were conducted using soybean (Glycine max var. Saba) as a model crop to test whether foliar application of zinc oxide nanoparticles (ZnO-NPs) or conventional Zn fertilizer (ZnSO4) would mitigate drought-related water stress and improve soybean yield. Each fertilizer was foliar applied twice at a two-week interval during the flowering stage. Experiments were concurrently conducted under non-drought conditions (70% field capacity) for comparison. Results showed drought significantly reduced relative water content, chlorophyll-a, and chlorophyll-b in untreated control plants by 35.7%, 47.7%, and 41.4%, respectively, compared to non-drought conditions (p < 0.05). Under drought conditions, ZnO-NPs (200mg Zn/L) led to 33.1% and 20.7% increase in chlorophyll-a and chlorophyll-b levels, respectively, compared to ZnSO4 at 400mg Zn/L. Likewise, catalase, peroxidase and superoxide dismutase activities increased by 62.6%, 39.5% and 28.5%, respectively, with ZnO-NPs (200mg Zn/L) under drought compared to non-drought conditions. Proline was significantly increased under drought but was remarkably suppressed (~ 54% lower) with ZnO-NPs (200mg Zn/L) treatment. More importantly, the highest seed yield was observed with ZnO-NPs (200mg Zn/L) treatment under drought (39% higher than untreated control) and non-drought (79.4% higher than control) conditions. Overall, the findings suggest that ZnO-NPs could promote seed yield in soybean under drought stress via increased antioxidant activities, increased relative water content, decreased stress-related proline content, and increased photosynthetic pigments. It is recommended that foliar application of 200mg Zn/L as ZnO-NPs could serve as an effective drought stress management strategy to improve soybean yield.

Traditional Use, Chemical Constituents, and Pharmacological Activity of Maytenus elaeodendroides Stem Bark

Plants belonging to the genus Maytenus are members of the Celastraceae family. They have been widely used by different peoples as treatment for curing many diseases. The aim of this study was to explore the anti-inflammatory and antioxidant properties of Maytenus elaeodendroides stem bark extracts, an endemic Cuban plant. The antioxidant activity of four extracts (EtOH, EtOAc, n-BuOH, and diethyl ether/petroleum ether 1:1) was determined using DPPH and FRAP methods. Meanwhile, anti-inflammatory effects by the edema method were induced by croton oil in the mouse ear. The investigated extracts showed radical reduction capacity and prevented ear inflammation at doses of 4 mg/ear. In addition, FIA/ESI/IT/MSn was used to determine the qualitative chemical composition of the EtOAc extract and allowed the identification of five flavan-3-ol monomers, four dimers, and other proanthocyanidin oligomers. From this extract three flavan-3-ol compounds (elaeocyanidin and 4′-O-methylgallocatechin), one of them new (2′-hydroxy-4′-methoxy-epigallocatechin), and a proanthocyanidin dimer (afzelechin-(4β→8)-4′-O-methylepigallocatechin) were isolated and identified by the chromatographic method and spectroscopic techniques, mainly ESI-MS and NMR spectroscopic methods.

Comparative Study of Essential Oils Extracted From Foeniculum vulgare Miller Seeds Using Hydrodistillation, Steam Distillation, and Superheated Steam Distillation

ABSTRACTFoeniculum vulgare Miller is a highly valued aromatic and nutritious plant. The unique compositions of its essential oil make it more valuable in the flavor, fragrance, and medicinal industries. However, the potential of superheated steam distillation for obtaining essential oils from its seeds has not been explored in detail. This study assessed the composition, yield, antimicrobial, and antioxidant activities of essential oils distilled from F. vulgare seeds using traditional hydrodistillation, steam distillation, and superheated steam distillation. Superheated steam distillation resulted in the maximum quantity of essential oil (5.24%) compared to steam (3.47%) and hydrodistillation (2.47%). Trans‐anethole, fenchone, estragole, and limonene were the main identified by GC–MS analysis in the essential oils, and these compounds were abundant in the essential oil produced by superheated steam distillation. Essential oil distilled by superheated steam distillation presented the highest antibacterial activity against Staphylococcus aureus, Pastrulla multocida, Bacillus subtilis, and Escherichia coli. The highest antifungal activity against Aspergillus niger, Fusarium solani, Aspergillus flavus, and Alternaria alternate was also demonstrated by the same essential oil. These findings demonstrated the potential of superheated steam distillation as a highly effective and efficient technique for distilling high‐quality essential oils from Foeniculum vulgare Miller seeds. It is suitable for various applications in the food, cosmetic, and pharmaceutical industries.

Evaluation and character association study for tuber yield, biochemical and antioxidant properties of sweet potato (Ipomoea batatas)

A study was carried out during 2022 and 2023 at ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya to evaluate the yield, biochemical and antioxidant properties of 49 accessions of sweet potato [Ipomoea batatas (L.) Lam.] in mid hill condition of north-eastern region. The experiment was laid out in randomized block design (RBD) with 3 replications. Results indicate that Mynthlu Orange exhibited the highest tuber weight, length, diameter and yield. In terms of biochemical parameters, Meghalaya Local recorded the highest dry matter content; Col-6 recorded the highest starch content; and X-24 had the highest total sugar content. Among antioxidants, X-24 showed the highest total phenolic content, highest FRAP assay value and anthocyanin with lowest IC50 value which signified that X-24 had the highest antioxidant activity. Correlation study revealed significant positive correlation of tuber yield with tuber weight, diameter and total anthocyanin. Based on the mean performance, accessions Mynthlu Orange, X-24 and Col-6 were found promising for yield, biochemical and antioxidant parameters.

Structure and characterization of an extracellular polysaccharide from Paenibacillus polymyxa 88A.

Levan-type polysaccharides, produced by various organisms, are nontoxic, biocompatible, and biodegradable polymers with a wide range of biological activities. They have high potential for use in medicine, cosmetology, and industry. A large amount of levan (41.1 g L-1) was recovered by ethanol precipitation from a liquid nutrient medium of Paenibacillus polymyxa 88A that contained 15 % w/v sucrose as a carbon source. The levan was fractionated by gel-permeation and anion-exchange chromatography and was analyzed by DRIFT and NMR spectroscopy. It was found that levan was represented by slightly branched chains composed of β-(2→6)-Fruf residues. The average molecular mass of the levan was about 1.9 MDa. When shear stress was applied at different temperatures, aqueous levan solutions showed pseudoplastic behavior. As found by SEM, a freeze-dried powdered levan sample had a microporous structure. The levan had excellent emulsifying activity toward sunflower oil, forming an emulsion with long-term stability. Analysis of the antioxidant activity of the levan showed a higher, dose-dependent activity toward ABTS, as compared with that toward DPPH. Finally, the bioactivity of the levan was examined by MTT assay by using human cervical carcinoma (HeLa) cells.

Chemical characterization of wheat-based waste derived from a pharmaceutical process for its potential valorization

IntroductionWe report the analysis and characterization and the preliminary biological evaluation, of both liquid and solid wastes obtained from the processing of wheat (Triticum vulgare) to produce the most iconic phytostimulin-based pharmaceutical products. The study aims to verify whether the waste can be reused in another process and not destined to its simple destructive disposal.MethodsIn this perspective, we first carried out an in-depth chemical-physical analysis of the waste together with a biocompatibility evaluation to plan the feasible final choice of waste destination. The liquid and solid waste derived from the processing of wheat extract were analyzed and characterized through ultra-high-performance liquid chromatography coupled with high-resolution Orbitrap mass spectrometry (UHPLC-Q-Orbitrap HRMS).ResultsResults highlight that ferulic acid represent the most abundant phenolic compound for solid waste with a content of 89.782 mg/kg and dihydroferulic acid is the predominant for liquid waste (6.24 mg/L). These concentrations represent 55.87% and 84.39% of the total concentration of bioactive compounds for liquid and solid waste, respectively. The antioxidant activity registered for the solid extract was 8.598 and 7.262 mmol trolox/kg, respectively for ABTS and FRAP assays. The total phenolic content (TPC) in the liquid extract undergoes a significant percentage reduction compared to the solid waste. As regards toxicity, both liquid and solid wastes were investigated in vitro preclinical models of human skin (HaCaT cells and HDFa) after 24, 48, and 72 h of exposure. No cytotoxic effect was noted even at the highest tested concentration (100 μg/mL) at 72 h.DiscussionOverall, considering its chemo-physical features and active ingredients, we believe that this waste is highly reusable as a starting material for the development of cosmeceutical products. Thus, this study allows us to motivate the destination of the waste of the production in a recyclable raw material for additional industrial processes, thereby promoting an eco-friendly circular economy operation.

Pengaruh Penambahan Ubi Jalar Kuning (Ipomoea Batatas L.) dan Bunga Rosella (Hibiscus Sabdariffa L.) Terhadap Karakteristik Fisikokimia dan Organoleptik Saus Tomat

Abstract. Tomato sauce is a product produced from a mixture of tomato puree or tomato paste or tomato solids obtained from ripe tomatoes, which is processed with spices, with or without the addition of other food ingredients and permitted food additives. The addition of yellow sweet potato was chosen as a natural thickener and rosella flowers to replace the role of synthetic dyes in tomato sauce. The research design used in this study was a randomized factorial block design consisting of 2 factors, namely the addition of yellow sweet potato (0%,15% and 30%) and the addition of rosella flowers (0%, 5% and 10%). Parameters tested are water content, total dissolved solids, pH, total carotene, antioxidant activity, color intensity and organoleptic. The results showed that there was an interaction between the addition of yellow sweet potato and rosella flower on water content, total dissolved solids, pH, antioxidant activity. The addition of yellow sweet potato affected the water content, total dissolved solids, pH, total carotene, antioxidant activity, brightness (L) and redness (a+). The addition of rosella flowers affected the water content, total dissolved solids, pH, total carotene, antioxidant activity and brightness (L). The best treatment was A2B1 with a value of water content (63.32%), total dissolved solids (19.33°Brix), pH (3.89), total carotene (22.32µg), antioxidant activity (76.71%), color intensity (L 39.63; a* 15.6; b* 16.2); aroma 4.24 (rather delicious), color 4.12 (rather bright), taste 4.56 (delicious), texture 4.88 (thick), preference 4.36 (rather like).

Antioxidant and anticancer activities of hesperetin and its novel formulations in KB cells.

This study aimed to formulate the hesperetin nanostructured lipid carriers (NLCs) containing oro-mucosal gel for its activity assessment on the KB cell line. NLCs were prepared with glyceryl monostearate, oleic acid, and lecithin using a modified constant-temperature emulsification technique. The particle size analysis, in vitro drug release studies, etc., of prepared NLCs were evaluated. The formulated gels were analyzed with respect to spreadability, extrudability, swelling index, texture analysis, etc. The particle size, polydispersity index, zeta potential, and drug entrapment of nanocarriers were recorded to be 221.733 ± 61.536nm, 0.381 ± 0.091, - 51.433 ± 4.143mV, and 89.29%, respectively. The optimized NLCs in 24h released 87.14 ± 6.62% of the drug. The round shape of NLCs was noticed with scanning electron microscopy. The pH, spreadability, extrudability, swelling index, content uniformity, and drug release studies of hesperetin NLCs-containing gel (HNG) were found to be 6.81 ± 0.04, 2.49 ± 0.04cm.mg/s, 539.04 ± 32.88g/cm2, 4.27 ± 0.47, 107.98 ± 1.93%, and 90.17 ± 6.67% (in 48h), respectively. The developed formulations showed promising in vitro anticancer and antioxidant activities. HNP results authorize that the formulation may be beneficial for the treatment of oral cancer.

Multi-Indicator Comprehensive Quality Evaluation of Turpinia arguta (Lindl.) Seem Herbs at Different Harvesting Periods

This study aimed to investigate differences in the leaf biomasses, chemical compositions, and pharmacological activities of Turpinia arguta histocultured seedlings and live seedlings at different harvesting periods (S1, S2, S3, and S4) in order to determine the optimal harvesting period. Eleven indexes, including biomass, key active components, secondary metabolites, nutrient content, and antioxidant activity, were evaluated by high-performance liquid chromatography and colorimetric methods during different harvesting periods. The weights of the 11 indexes were calculated by principal component analysis, and then a comprehensive quality evaluation was performed. The results showed significant differences in leaf biomasses, key active components, secondary metabolites, nutrient contents, and antioxidant activities between the different harvesting periods. The highest quality score was obtained for the S4 period, indicating that the quality of T. arguta was the best at this time and that the S4 period was the most suitable harvesting period. At this period (S4), the medicinal component content, antioxidant activity, and comprehensive quality score of the histocultured seedlings of T. arguta were higher than those of the live seedlings, indicating the importance of tissue culture technology in enhancing the quality of T. arguta. This study provides more novel and abundant information and reference for determining the appropriate harvesting period of T. arguta, with the aim of providing newer scientific guidance for the management of herbs.

Temperature Effect of Cocoa (Theobroma cacao L.) Drying on Energy Consumption, Bioactive Composition and Vibrational Changes

Cocoa drying is the post-harvest thermal process used to condition the beans to a moisture content between 6.5 and 7% for storage and further processing. Convective drying is an energy-intensive process where time and temperature are considered critical factors for the degradation of bioactive compounds in edible products. In the present study, the energy parameters, vibrational spectroscopy, and changes in bioactive compounds of cocoa beans were studied during thin-layer hot air drying at 50 °C, 60 °C, and 70 °C. Moisture loss, specific energy consumption (SEC), energy efficiency, total phenolics (TPs), total flavonoids (TFs), and antioxidant activity (DPPH) were determined. Fourier transform infrared (FT-IR) spectroscopy with attenuated total reflectance (ATR) was used to characterize the samples, and a multivariate analysis was applied to find interactions among the components. The obtained SEC was 18,947.30–24,469.51 kJ/kg, and the energy efficiency was 9.73–12.31%. When the temperature was 70 °C, the best values for SEC and energy efficiency were obtained. The results also showed that the convective drying generated changes in the TP levels for the three temperatures, mainly after 300 min, with maximum levels between 360 and 600 min, at 70 °C; however, it does not have a clear relationship with the TFs and the antioxidant activity. The FT-IR and the multivariate analysis revealed changes in several signals in the 1800 to 400 cm−1 range, confirming the variation in the associated signal with phenolic compounds.

Determination of antioxidant activity in milk extracts with phosphomolibdate method

Antioxidants are mainly non-nutrient compounds in both human and animal feed, but have the antioxidant capacity in vitro to provide an artificial power index in preventing the destruction of cells and tissue potential by inhibiting nutrient oxidation. Milk contains lipophilic and hydrophilic antioxidants, which play a key role in maintaining. The efficiency of extraction for determining the antioxidant activity of milk corresponds to the method used for plant extraction. For this purpose, the phosphor molybdenum method based on the reduction of Mo (VI) in Mo (B) in samples of milk. Extracts obtained with methanol and ethanol, Soxlet method. Green complexes formed at acidic pH value, measurements of spectrophotometer (UV), λ = 695 nm. The values of milk samples are compared with respect to the calibration curve of gallic acid range (0.00 to 14.00 μg / ml, y = 0.0344 + 0.0519, R2 = 0.9709). The results of milk extract was concentration/ c = 3, 80; 2.35; 3.78; 4.85 / (μg / ml). The highest value of antioxidant activity in packs milk, which has 3.2% fat, also affects the fat and the presence of vitamin E, which found in fat droplets and has a synergistic effect with vitamin C. It concluded that the highest value of the total antioxidant activity in milk obtained from the first sample due to the use of several types of feed – alfalfa, two types of concentrated and straw, which proves the dependence of antioxidant activity on the impact of nutrients, that is, of their type and quantities.

Assessing the efficacy of pearl millet and finger millet enriched breakfast cereals to combat obesity.

This research explores the development and evaluation of breakfast cereals formulated from pearl millet (P-1) and finger millet (F-1) flours, with a focus on their nutritional composition and potential anti-obesity properties. The techno-functional properties were assessed, revealing that P-1 exhibited a bulk density of 0.40 ± 0.01g/ml and a true density of 0.61 ± 0.03g/ml, while F-1 showed a bulk density of 0.54 ± 0.03g/ml and a true density of 0.65 ± 0.02g/ml. The Carr index and Hausner ratio were significantly higher in P-1 (40.33 ± 0.41 and 1.68 ± 0.03, respectively) compared to F-1 (22.3 ± 0.3 and 1.31 ± 0.02, respectively). Both flours demonstrated comparable water absorption indices, but P-1 had superior foaming capacity and stability. Nutritional analysis indicated that P-1 contained 12.64% moisture, 6.02% fat, 10.29% protein, 63.94% carbohydrates, 2.38% ash, and 2.75% fibre, whereas F-1 had 8.92% moisture, 1.78% fat, 8.65% protein, 73.65% carbohydrates, 1.77% ash, and 4.04% fibre. Composite flours (PF-1, PF-2, PF-3) exhibited varying compositions: PF-1 had 9.86% moisture, 5.83% fat, 11.62% protein, 66.04% carbohydrates, 2.19% ash, and 3.03% fibre; PF-2 had 10.79% moisture, 3.87% fat, 10.01% protein, 68.97% carbohydrates, 2.05% ash, and 3.37% fibre; PF-3 had 9.87% moisture, 2.84% fat, 9.33% protein, 71.12% carbohydrates, 1.92% ash, and 3.74% Fiber. Antioxidant activity was higher in F-1 (80.63%) compared to P-1 (59.58%), with PF-1, PF-2, and PF-3 showing 64.84%, 70.14%, and 75.4%, respectively. Lipase and amylase inhibition capacities were 73.15% and 28.66% for P-1, 28.31% and 30.65% for F-1, and varied for composites: PF-1 (79.12% lipase, 46.41% amylase), PF-2 (50.75% lipase, 53.15% amylase), and PF-3 (39.51% lipase, 41.84% amylase). These findings underscore the distinct nutritional and functional properties of these flours, highlighting their potential in obesity management.

Development of a Novel Gluten-Free Cookie Premix Enriched with Natural Flours Using an Extreme Vertices Design: Physical, Sensory, Rheological, and Antioxidant Characteristics

This work aimed to develop novel alternative gluten-free premixes for use in the production of cookies by optimizing the formulation of three different starches (corn, potato, and tapioca starch) in a rice/corn formula and then enriching the optimized formula with various natural flours (acorn, soy, chestnut, chickpea, millet, and quinoa). An extreme vertices mixture design was used to optimize the mixing sub-part consisting of 30% (w/w) of starches. The enriched gluten-free cookies were characterized by physical, rheological, antioxidant, color, and sensory properties. An optimum was obtained by mixing 18.5084 g of corn starch, 6 g of potato starch, and 5.4916 g of tapioca starch. The enrichment results indicated that soy-enriched gluten-free cookies have a high specific volume (2.428 cm3/g) with the highest spread ratio (12.25) compared to other cookies. The rheological properties of enriched gluten-free doughs indicated a higher value of the consistency coefficient (K) of soy-enriched gluten-free cookies. The antioxidant characteristics were enhanced by replacing corn with several types of flours, with higher TPC for acorn (2.83 mg GAE/g dw) and soy-enriched cookies (2.49 mg GAE/g dw) with better antioxidant activities (DPPH, ABTS, and RED). The tasters gave all the cookies favorable ratings for overall acceptability. With an average rating of 7.09, cookies enhanced with chickpea flour have a high acceptability compared to the other cookies. Considering all the tested parameters, PCA analysis clustered millet, corn, quinoa, and chestnut gluten-free enriched cookies into the same group. However, acorn and soy cookies were placed in a separate group, and chickpea-enriched cookies were classified separately.

Exploring time-killing and biofilm inhibition potential of bioactive proteins extracted from two varieties of Pleurotus ostreatus

IntroductionDental caries, caused by oral microbial pathogens, are a global health concern, further exacerbated by the presence of methicillin-resistant Staphylococcus aureus (MRSA). Bioactive proteins and peptides (BAPs) exhibit potent antimicrobial properties, targeting multiple cellular mechanisms within pathogens, reducing the likelihood of resistance development. Given the antimicrobial potential of BAPs, this study aimed to compare the efficacy of BAPs extracted from cultivated (Pleurotus ostreatus, PoC) and wild (Pleurotus ostreatus, PoW) mushrooms against pathogens responsible for dental caries.MethodsBAPs were extracted from both PoC and PoW using a TCA-acetone method. Antimicrobial activities were tested against seven bacteria and one fungus using agar well diffusion and MIC determination. Antibiofilm activity was assessed via modified CV assay, while DPPH and erythrocyte lysis tests evaluated free radical scavenging.ResultsPoC showed superior antimicrobial efficacy, with lower MIC and MBC values, and disrupted biofilm integrity at increasing concentrations. PoW exhibited better antioxidant activity with higher DPPH scavenging, though its antimicrobial efficacy was slightly lower than PoC.DiscussionBoth PoC and PoW BAPs inhibited dental pathogens, with PoC showing stronger inhibition against MRSA and nystatin-resistant Candida albicans. This suggests BAPs may target additional cellular mechanisms beyond membranes, PBPs, and ergosterols. Despite PoW’s stronger antioxidant properties, both BAPs had comparable antibiofilm activity. These findings suggest complementary actions of BAPs from PoC and PoW both, in treating dental caries, offering broad-spectrum antimicrobial and antioxidant benefits.