Antimicrobial Metal Research Articles

Antibacterial and Antiviral Effects of Ag, Cu and Zn Metals, Respective Nanoparticles and Filter Materials Thereof against Coronavirus SARS-CoV-2 and Influenza A Virus.

Due to the high prevalence of infectious diseases and their concurrent outbreaks, there is a high interest in developing novel materials with antimicrobial properties. Antibacterial and antiviral properties of a range of metal-based nanoparticles (NPs) are a promising means to fight airborne diseases caused by viruses and bacteria. The aim of this study was to test antimicrobial metals and metal-based nanoparticles efficacy against three viruses, namely influenza A virus (H1N1; A/WSN/1933) and coronaviruses TGEV and SARS-CoV-2; and two bacteria, Escherichia coli and Staphylococcus aureus. The efficacy of ZnO, CuO, and Ag NPs and their respective metal salts, i.e., ZnSO4, CuSO4, and AgNO3, was evaluated in suspensions, and the compounds with the highest antiviral efficacy were chosen for incorporation into fibers of cellulose acetate (CA), using electrospinning to produce filter materials for face masks. Among the tested compounds, CuSO4 demonstrated the highest efficacy against influenza A virus and SARS-CoV-2 (1 h IC50 1.395 mg/L and 0.45 mg/L, respectively), followed by Zn salt and Ag salt. Therefore, Cu compounds were selected for incorporation into CA fibers to produce antiviral and antibacterial filter materials for face masks. CA fibers comprising CuSO4 decreased SARS-CoV-2 titer by 0.38 logarithms and influenza A virus titer by 1.08 logarithms after 5 min of contact; after 1 h of contact, SARS-COV-2 virus was completely inactivated. Developed CuO- and CuSO4-based filter materials also efficiently inactivated the bacteria Escherichia coli and Staphylococcus aureus. The metal NPs and respective metal salts were potent antibacterial and antiviral compounds that were successfully incorporated into the filter materials of face masks. New antibacterial and antiviral materials developed and characterized in this study are crucial in the context of the ongoing SARS-CoV-2 pandemic and beyond.

MEGARes and AMR++, v3.0: an updated comprehensive database of antimicrobial resistance determinants and an improved software pipeline for classification using high-throughput sequencing.

Antimicrobial resistance (AMR) is considered a critical threat to public health, and genomic/metagenomic investigations featuring high-throughput analysis of sequence data are increasingly common and important. We previously introduced MEGARes, a comprehensive AMR database with an acyclic hierarchical annotation structure that facilitates high-throughput computational analysis, as well as AMR++, a customized bioinformatic pipeline specifically designed to use MEGARes in high-throughput analysis for characterizing AMR genes (ARGs) in metagenomic sequence data. Here, we present MEGARes v3.0, a comprehensive database of published ARG sequences for antimicrobial drugs, biocides, and metals, and AMR++ v3.0, an update to our customized bioinformatic pipeline for high-throughput analysis of metagenomic data (available at MEGLab.org). Database annotations have been expanded to include information regarding specific genomic locations for single-nucleotide polymorphisms (SNPs) and insertions and/or deletions (indels) when required by specific ARGs for resistance expression, and the updated AMR++ pipeline uses this information to check for presence of resistance-conferring genetic variants in metagenomic sequenced reads. This new information encompasses 337 ARGs, whose resistance-conferring variants could not previously be confirmed in such a manner. In MEGARes 3.0, the nodes of the acyclic hierarchical ontology include 4 antimicrobial compound types, 59 resistance classes, 233 mechanismsand 1448 gene groups that classify the 8733 accessions.

SourceFinder: a Machine-Learning-Based Tool for Identification of Chromosomal, Plasmid, and Bacteriophage Sequences from Assemblies.

High-throughput genome sequencing technologies enable the investigation of complex genetic interactions, including the horizontal gene transfer of plasmids and bacteriophages. However, identifying these elements from assembled reads remains challenging due to genome sequence plasticity and the difficulty in assembling complete sequences. In this study, we developed a classifier, using random forest, to identify whether sequences originated from bacterial chromosomes, plasmids, or bacteriophages. The classifier was trained on a diverse collection of 23,211 chromosomal, plasmid, and bacteriophage sequences from hundreds of bacterial species. In order to adapt the classifier to incomplete sequences, each complete sequence was subsampled into 5,000 nucleotide fragments and further subdivided into k-mers. This three-class classifier succeeded in identifying chromosomes, plasmids, and bacteriophages using k-mer distributions of complete and partial genome sequences, including simulated metagenomic scaffolds with minimum performance of 0.939 area under the receiver operating characteristic curve (AUC). This classifier, implemented as SourceFinder, has been made available as an online web service to help the community with predicting the chromosomal, plasmid, and bacteriophage sources of assembled bacterial sequence data (https://cge.food.dtu.dk/services/SourceFinder/). IMPORTANCE Extra-chromosomal genes encoding antimicrobial resistance, metal resistance, and virulence provide selective advantages for bacterial survival under stress conditions and pose serious threats to human and animal health. These accessory genes can impact the composition of microbiomes by providing selective advantages to their hosts. Accurately identifying extra-chromosomal elements in genome sequence data are critical for understanding gene dissemination trajectories and taking preventative measures. Therefore, in this study, we developed a random forest classifier for identifying the source of bacterial chromosomal, plasmid, and bacteriophage sequences.

Antimicrobial resistance profile of Escherichia coli isolated from poultry litter

Antimicrobial resistance is a threat to animal and human health. As a commensal and zoonotic bacterium, Escherichia coli has the potential to be a pathogenic source of antimicrobial resistance. The purpose of this study aimed to investigate the antimicrobial resistance profile of E. coli isolated from litter collected from pens in a broiler chicken experiment. E. coli was isolated from litter samples (n=68 isolates) of 16 pens housing broilers to d 53 of age. Resistance to 10 antimicrobials was observed by disc diffusion. The presence of 23 antimicrobial and heavy metal resistance genes, O serogroups, and avian pathogenic E. coli (APEC-like) minimal predictor genes were identified through PCR. E. coli isolates presented the greatest resistance to cephalothin (54.4%), tetracycline (27.9%), streptomycin (29.4%), ampicillin (20.6%), colistin (13.2%), sulphonamides (8.8%), and imipenem (1.5%). Multidrug resistance to at least 3 antimicrobials was observed in 22.1% of isolates. The identified O-types of the E. coli isolates were O15, O75, O78, and O91. There was a greater likelihood that the genes groEL, aph(3)IA, silP, sull, aadA, qacEdelta1, iroN, ompTp, and hlyF were present in isolates that exhibited ampicillin resistance (P ≤ 0.05). There was a greater likelihood that the groEL gene was present in isolates resistant to ampicillin, colistin, tetracycline, sulphonamides, or cephalothin (P ≤ 0.05). Further characterizing E. coli antimicrobial resistance is essential and aids in developing effective solutions, thereby furthering the One Health objective.

Isolation of exopolysaccharide, galactan from marine Vibrio sp. BPM 19 to template the synthesis of antimicrobial platinum nanocomposite

Herein, a galactan exopolysaccharide (EPS) producing marine bacteria Vibrio Sp. BPM19 was reported. Phylogenetic analysis based on 16S rRNA gene sequencing showed the proximity of the isolate to Vibrio alginolyticus strain 1306. Gel permeation chromatography revealed the molecular weight of the purified EPS was about 6342.3 Daltons. 1H NMR, 13C NMR, and FTIR analysis indicated that isolated EPS comprises high molecular weight polysaccharides. HPLC analysis suggests that the EPS is composed of a repeating unit of galactose. DSC-TGA studies support that the EPS has very high thermal stability. The potential application of purified EPS in nanotechnology was demonstrated by the synthesis of platinum nanocomposite (PtNC). The PtNC was characterized by energy-dispersive X-ray diffraction, transmission electron microscopy, zeta potential, particle size analysis, and FTIR. The antimicrobial potential of the PtNC was demonstrated against drug-resistance Salmonellae typhi and Staphylococcus aureus. The result suggested the possible commercial scope of the isolated strain to produce galactan, which can synthesize antimicrobial metal nanocomposite.

Copper-doped zeolitic imidazolate frameworks-8/hydroxyapatite composite coating endows magnesium alloy with excellent corrosion resistance, antibacterial ability and biocompatibility

Magnesium (Mg) and its alloys exhibit an excellent prospect for orthopedic clinical application due to their outstanding biodegradability and mechanical adaptability. However, the rapid corrosion rate/latent device-associated infections may lead to a failed internal fixation of Mg-based implants. Herein, a novel composite coating consisted of outer copper-doped zeolitic imidazolate frameworks-8 and inner hydroxyapatite (Cu@ZIF-8/HA) was in situ constructed on AZ31B Mg alloy via a two-step approach of hydrothermal treatment and seeded solvothermal method. The results verified that the electrochemical impedance of the obtained Cu45@ZIF-8/HA composite coating increased by two orders of magnitude to 6.6013 × 104 Ω·cm2 compared to that of bare Mg alloy. This was attributed to the reduced particle size of ZIF-8 nanoparticles due to the doped copper ions, which could be effectively grown in situ on the micro-nano flower-like structure of the HA-coated Mg alloy. Meanwhile, the Cu@ZIF-8/HA coating exhibited excellent antibacterial properties due to the release of copper ions and zinc ions from Cu@ZIF-8 dissolved in bacterial culture solution. The ICP results unraveled that the released concentration of copper and zinc ions could enhance the activity of alkaline phosphatase in the appropriate range during MC3T3-E1 cell culture in vitro for 7 days. This research revealed that the preparation of multifunctional metal-organic frameworks coating doped with antimicrobial metal ions via the seed layer solvothermal method was significant for studying the antimicrobial properties, osteogenic performance and corrosion resistance of Mg-based bioactive coatings.

Multicomponent Polymerization of Azides, Alkynes, and Electrophiles toward 1,4,5-Trisubstituted Polytriazoles

As a model click reaction, alkyne–azide cycloaddition (AAC) has been widely applied in polymer and materials sciences. The corresponding alkyne–azide polymerization is a powerful approach for preparing polytriazoles with versatile functionalities such as antimicrobial activity, heat resistance, and metal coordination. However, it still remains a great synthetic challenge for the structural modification of polytriazoles due to the relative inert functional groups. Herein, inspired by the specific electrophiles in the interrupted AAC reaction to synthesize 5-functionalized triazoles, a multicomponent polymerization (MCP) using diynes, diazides, and electrophiles was developed, affording a library of 1,4,5-trisubstituted polytriazoles (1,4,5-PTAs) with high yields (up to 95%) and molecular weights (up to 280 100 g/mol). As the third component, five electrophiles were screened to optimize the ratio of 1,4,5-PTAs to non-functionalized 1,4-PTAs (up to 95% 1,4,5-PTAs were determined by 1H NMR). To improve the solubility of the obtained products, diynes and diazides containing flexible chains were selected to prepare amphiphilic 1,4,5-PTAs, which could self-assemble into nanoparticles in water using the nanoprecipitation method. As nanocatalysts, such nanoparticles were further applied in the aqueous AAC reaction with excellent catalytic efficiency at a low Cu loading of 50 ppm. The introduction of the third component bearing chelating sites would stabilize Cu(I) under air conditions, exhibiting enhanced catalytic efficiency without additional reducing agents compared to nonfunctionalized PTAs.

Silicon supplementation induces expression of a histidine-rich defensin gene family in Nicotiana tabacum

Silicon (Si) is known to protect plants from a variety of environmental and biological stressors. The mode of action for these Si responses is unclear. In this study, a family of 14 histidine-rich defensin genes (HRDs) in Nicotiana tabacum, previously described as Defensin 19-like were identified and induced following Si treatment. These genes formed the Nicotiana tabacum histidine-rich defensin 1 (NtHRD1) family. All but one of the NtHRD1 genes were induced in roots with Si treatment, with different members showing differential regulation in upper and lower roots. More than 60% of the NtHRD1 genes showed induced expression with Si treatment in leaves from the upper portion of the plant, while only two genes showed induced expression in lower leaves. Histidine-rich defensins are a newly described group of plant cis-defensins that may play roles in both antimicrobial activity and metal binding. Induction of these genes in N. tabacum following Si treatment could explain some of the beneficial properties of this nutrient.

Amalgamation and Scrutinizing of Leucine Derivatives Schiff Bases Complexes as Antimicrobial Agent.

The enhanced applications of Schiff bases metal complexes of amino acid derivatives have captured the attention of researchers for the synthesis of leucine derivatives of Schiff bases metal complexes. Amino acids are considered to be essential part of food supplements as well as derivatives of Schiff bases in coordination chemistry due to their donor ability. The leucine derivatives Schiff bases ligand have been synthesized by condensation reaction between amine of leucine with aldehyde or ketone bearing molecules attached with them. These complexes were characterized by different spectroscopic tools in order to confirm their structural geometries. The structural geometries are considered to be very important in order to improve the antimicrobial potential of leucine derivative metal complexes. By taking into account the antimicrobial potential of titled compounds, a comprehensive review of leucine derivatives of Schiff bases metal complexes has been compiled.

Occurrence and genetic characteristics of multidrug-resistant Escherichia coli isolates co-harboring antimicrobial resistance genes and metal tolerance genes in aquatic ecosystems.

Multidrug-resistant (MDR) Escherichia coli isolates (n=50) were recovered from aquatic ecosystems, which presented high counts of E. coli and metal values within the recommended range. These isolates showed different multidrug resistance profiles, highlighting the resistance to extended-spectrum cephalosporins, polymyxins, and fluoroquinolones. Several antimicrobial resistance genes (ARGs) were found, spotlighting the presence of at least one β-lactamase-encoding gene in each E. coli isolate. Substitutions in the quinolone resistance-determining regions and the two-component systems involving PhoP/PhoQ and PmrA/PmrB were also found. The metal tolerance gene rcnA (nickel and cobalt efflux pump) was the most prevalent. In this regard, 94% of E. coli isolates presented the co-occurrence of at least one ARG and metal tolerance gene. Furthermore, virulence genes and genetic diversity were found among MDR E. coli isolates. The emergence of potentially pathogenic isolates exhibiting multidrug resistance and metal tolerance emerged as a global health problem at the human-animal-environment interface.

Pseudomonas aeruginosa from pet Chinese stripe-necked turtles (Ocadia sinensis) demonstrating antimicrobial and heavy metal resistance

Leading nosocomial pathogen Pseudomonas aeruginosa has increasingly been reported to be an opportunistic pathogen. In this study, a total of twenty P. aeruginosa isolates were isolated from 40 pet Chinese stripe-necked turtles and examined for their antimicrobial and heavy metal resistance properties. All isolates were multidrug resistance by scoring multiple antimicrobial resistance indices ≥0.2. In the disc distribution test, 100% resistance to ampicillin and oxacillin were detected. In addition to that, 14 (70%) isolate demonstrated amoxicillin resistance. Imipenem, fosfomycin, gentamycin, tobramycin and piperacillin resistance were detected in 40%, 15%, 20%, 10% and 5% of the isolates, respectively. The ESBLs gene that predominated in this study was blaSHV (55%), followed by blaTEM (50%), blaCTX (10%) and blaOXA (5%). The most frequent aminoglycoside resistance gene in this study was aac(6´)-Ib (40%). Class1 integron integrase gene intI1 and class 1 integron gene cassette gene aadA1 were detected in 45% and 35% of the isolates, respectively. All P. aeruginosa isolates demonstrated Cu and Cd resistance. CzcA and CopA genes were detected in 65% and 30% of the isolates, respectively. These findings reveal the presence of pet turtle-born P. aeruginosa can be a potential risk to public health and cannot be excluded as a non-nosocomial source of infections.

MIL-101 (Fe) @Ag Rapid Synergistic Antimicrobial and Biosafety Evaluation of Nanomaterials.

Metal-organic frameworks (MOFs), which have become popular in recent years as excellent carriers of drugs and biomimetic materials, have provided new research ideas for fighting pathogenic bacterial infections. Although various antimicrobial metal ions can be added to MOFs with physical methods, such as impregnation, to inhibit bacterial multiplication, this is inefficient and has many problems, such as an uneven distribution of antimicrobial ions in the MOF and the need for the simultaneous addition of large doses of metal ions. Here, we report on the use of MIL-101(Fe)@Ag with efficient metal-ion release and strong antimicrobial efficiency for co-sterilization. Fe-based MIL-101(Fe) was synthesized, and then Ag+ was uniformly introduced into the MOF by the substitution of Ag+ for Fe3+. Scanning electron microscopy, powder X-ray diffraction (PXRD) Fourier transform infrared spectroscopy, and thermogravimetric analysis were used to investigate the synthesized MIL-101(Fe)@Ag. The characteristic peaks of MIL-101(Fe) and silver ions could be clearly seen in the PXRD pattern. Comparing the diffraction peaks of the simulated PXRD patterns clearly showed that MIL-101(Fe) was successfully constructed and silver ions were successfully loaded into MIL-101(Fe) to synthesize an MOF with a bimetallic structure, that is, the target product MIL-101(Fe)@Ag. The antibacterial mechanism of the MOF material was also investigated. MIL-101(Fe)@Ag exhibited low cytotoxicity, so it has potential applications in the biological field. Overall, MIL-101(Fe)@Ag is an easily fabricated structurally engineered nanocomposite with broad-spectrum bactericidal activity.

ICP-MS Determination of Antimicrobial Metals in Microcapsules.

Silver (Ag) and zinc (Zn) are very powerful antimicrobial metals. Therefore, in this research, a high-throughput, sensitive, and rapid method was developed for the determination of Ag and Zn in microcapsules using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple microwave digestion of the microcapsules with 55.55% v/v HNO3 and 44.45% v/v H2O2. The method was applied to determine Ag and Zn in microcapsule samples of different sizes (120 and 450 μm) after their preparation with and without chitosan. Prepared microcapsules, after characterization, were bonded to a polymer carrier by sol-gel procedure and the materials were characterized by FTIR spectroscopy and high-resolution optical microscopy. Significant differences were found in Ag and Zn levels between microcapsules samples prepared with and without chitosan. The results have shown that samples with chitosan had up to 20% higher levels of Zn than Ag: 120 μm microcapsules contained 351.50 μg/g of Ag and 85.51 μg/g of Zn, respectively. In contrast, samples prepared without chitosan showed larger overall variability: In microcapsules with a diameter of 120 μm, the amounts of antimicrobial metals were 98.32 μg/g of Ag and 106.75 μg of Zn, respectively. Moreover, 450 μm microcapsules contained 190.98 μg/g of Ag and 121.35 μg/g of Zn. Those quantities are high enough for efficient antimicrobial activity of newly prepared microcapsules, enabling the application of microcapsules in different antimicrobial coatings.

Advances in Nanoarchitectonics of Antimicrobial Tiles and a Quest for Anti-SARS-CoV-2 Tiles

Design of antimicrobial tiles seems necessary to combat against contagious diseases, especially COVID-19. In addition to personal hygiene, this technology facilitates public hygiene as antimicrobial tiles can be installed at hospitals, schools, banks, offices, lobbies, railway stations, etc. This review is primarily focused on preparing antimicrobial tiles using an antimicrobial layer or coatings that fight against germs. The salient features and working mechanisms of antimicrobial tiles are highlighted. This challenge is a component of the exploratory nature of nanoarchitectonics, that also extends farther than the realm of nanotechnology. This nanoarchitectonics has been successful at the laboratory scale as antimicrobial metal nanoparticles are mainly used as additives in preparing tiles. A detailed description of various materials for developing unique antimicrobial tiles is reported here. Pure metal (Ag, Zn) nanoparticles and a mixture of nanoparticles with other inorganic materials (SiO2,, TiO2, anatase, nepheline) have been predominantly used to combat microbes. The developed antimicrobial tiles have shown excellent activity against a wide range of Gram-positive and Gram-negative bacteria. The last section discussed a hypothetical overview of utilizing the antimicrobial tiles against SARS-CoV-2. Overall, this review gives descriptive knowledge about the importance of antimicrobial tiles to create a clean and sustainable environment.Graphical

Evaluation of Water Quality of Buritis Lake

The implementation of natural parks in cities is a current and controversial theme. Therefore, in Lake Buritis, which is a leisure area for the population of the city of Goiatuba, Goiás, Brazil, the quality of the water was analyzed, carrying out quantitative tests of the water samples at six different points between the source and the outlet of the lake that flows into the stream, Chico À Toa. Physical–chemical tests (turbidity, pH, alkalinity, electrical conductivity, color, nitrate and hardness parameters), microbiological tests for species identification, analysis of the antimicrobial susceptibility profile, metals analysis and Allium cepa test, were performed. The total coliforms number in water samples was higher than the maximum value established by Brazilian legislation, demonstrating high fecal contamination during the spring in samples from an artesian well. The bacterial diversity found was large and there were many pathogenic bacteria. The A. cepa test demonstrated a cytotoxic potential for water from the source and outlet of Buritis Lake. Statistical tests were applied to verify existing correlations between parameters. Among the analyzed data, the highest correlation was between the color and turbidity parameters and the grouping between the metals (lead, iron, cadmium and magnesium).

Relationship and distribution of Salmonella enterica serovar I 4,[5],12:i:- strain sequences in the NCBI Pathogen Detection database

BackgroundOf the > 2600 Salmonella serovars, Salmonella enterica serovar I 4,[5],12:i:- (serovar I 4,[5],12:i:-) has emerged as one of the most common causes of human salmonellosis and the most frequent multidrug-resistant (MDR; resistance to ≥3 antimicrobial classes) nontyphoidal Salmonella serovar in the U.S. Serovar I 4,[5],12:i:- isolates have been described globally with resistance to ampicillin, streptomycin, sulfisoxazole, and tetracycline (R-type ASSuT) and an integrative and conjugative element with multi-metal tolerance named Salmonella Genomic Island 4 (SGI-4).ResultsWe analyzed 13,612 serovar I 4,[5],12:i:- strain sequences available in the NCBI Pathogen Detection database to determine global distribution, animal sources, presence of SGI-4, occurrence of R-type ASSuT, frequency of antimicrobial resistance (AMR), and potential transmission clusters. Genome sequences for serovar I 4,[5],12:i:- strains represented 30 countries from 5 continents (North America, Europe, Asia, Oceania, and South America), but sequences from the United States (59%) and the United Kingdom (28%) were dominant. The metal tolerance island SGI-4 and the R-type ASSuT were present in 71 and 55% of serovar I 4,[5],12:i:- strain sequences, respectively. Sixty-five percent of strain sequences were MDR which correlates to serovar I 4,[5],12:i:- being the most frequent MDR serovar. The distribution of serovar I 4,[5],12:i:- strain sequences in the NCBI Pathogen Detection database suggests that swine-associated strain sequences were the most frequent food-animal source and were significantly more likely to contain the metal tolerance island SGI-4 and genes for MDR compared to all other animal-associated isolate sequences.ConclusionsOur study illustrates how analysis of genomic sequences from the NCBI Pathogen Detection database can be utilized to identify the prevalence of genetic features such as antimicrobial resistance, metal tolerance, and virulence genes that may be responsible for the successful emergence of bacterial foodborne pathogens.

Healthcare-associated infections caused by chlorhexidine-tolerant Serratia marcescens carrying a promiscuous IncHI2 multi-drug resistance plasmid in a veterinary hospital.

The bacterium Serratia marcescens can cause opportunistic infections in humans and in animals. In veterinary settings, the diversity, reservoirs and modes of transmission of this pathogen are poorly understood. The phenotypes and genotypes of Serratia spp. isolated from dogs, cats, horses, a bird and a rabbit examined at an Australian veterinary hospital between 2008 and 2019 were characterised. The isolates were identified as S. marcescens (n = 15) or S. ureilytica (n = 3) and were placed into four distinct phylogenetic groups. Nine quasi-clonal isolates associated with post-surgical complications in different patients displayed high levels of resistance to the antimicrobials fluoroquinolones, cephalosporins, aminoglycosides, and to the disinfectant chlorhexidine. A Serratia sp. with a similar resistance profile was also isolated from chlorhexidine solutions used across the Hospital, suggesting that these infections had a nosocomial origin. A genomic island encoding a homolog of the Pseudomonas MexCD-OprJ biocide efflux system was detected in the chlorhexidine-tolerant Serratia. The nine multi-drug resistant Serratia isolates also possessed a Ser-83-Ile mutation in GyrA conferring fluoroquinolone resistance, and carried a large IncHI2 conjugative plasmid encoding antimicrobial and heavy metal resistances. This replicon was highly similar to a plasmid previously detected in a strain of Enterobacter hormaechei recovered from the Hospital environment. IncHI2 plasmids are commonly found in Enterobacteriaceae, but are rarely present in Serratia spp., suggesting that this plasmid was acquired from another organism. A chlorhexidine-tolerant Serratia isolate which lacked the IncHI2 plasmid was used in mating experiments to demonstrate the transfer of multi-drug resistance from a E. hormaechei donor. This study illustrates the importance of environmental surveillance of biocide-resistance in veterinary hospitals.

Synergistic Antimicrobial Metal Oxide-Doped Phosphate Glasses; a Potential Strategy to Reduce Antimicrobial Resistance and Host Cell Toxicity.

The emergence of antimicrobial resistant strains bacteria and a decline in the discovery of new antibiotics has led to the idea of combining various antimicrobials to treat resistant strains and/or polymicrobial infections. Metal oxide-doped glasses have been extensively investigated for their antimicrobial potential; however to date, most experiments have focused on single metal species in isolation. The present study investigates the antimicrobial potential of sodium calcium phosphates (P2O5)50(Na2O)20(CaO)30–X(MO)X, where M is cobalt, copper, or zinc as single species. In addition, this work studied the effect of co-doping glasses containing two different metal ions (Co + Cu, Co + Zn, and Cu + Zn). The antimicrobial efficacy of all glasses was tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains, as well as a fungal strain (Candida albicans). Minimum inhibitory and bactericidal concentrations and time kill/synergy assays were used to assess the antimicrobial activity. An enhanced antimicrobial effect, at 5 mg/mL concentration, was exhibited by cobalt, copper, and zinc oxide glasses alone and in combinations. A synergistic antimicrobial effect was observed by Cu + Co and Cu + Zn against E. coli and Cu + Zn against S. aureus.

Emerging and future strategies in the management of recalcitrant Candida auris.

Candida auris is an emerging, multi drug resistant fungal pathogen that has caused infectious outbreaks in over 45 countries since its first isolation over a decade ago, leading to in-hospital crude mortality rates as high as 72%. The fungus is also acclimated to disinfection procedures and persists for weeks in nosocomial ecosystems. Alarmingly, the outbreaks of C. auris infections in Coronavirus Disease-2019 (COVID-19) patients have also been reported. The pathogenicity, drug resistance and global spread of C. auris have led to an urgent exploration of novel, candidate antifungal agents for C. auris therapeutics. This narrative review codifies the emerging data on the following new/emerging antifungal compounds and strategies: antimicrobial peptides, combinational therapy, immunotherapy, metals and nano particles, natural compounds, and repurposed drugs. Encouragingly, a vast majority of these exhibit excellent anti- C. auris properties, with promising drugs now in the pipeline in various stages of development. Nevertheless, further research on the modes of action, toxicity, and the dosage of the new formulations are warranted. Studies are needed with representation from all five C. auris clades, so as to produce data of grater relevance, and broader significance and validity.Lay SummaryElimination of Candida auris that causes deadly infections to susceptible individuals is extremely challenging due to the lack of effective treatment options. Promising, new antifungal agents and strategies are being developed and further refinement will facilitate their clinical use in the near future.

Antibacterial lignin-based nanoparticles and their use in composite materials.

Lignin, one of the most abundant biopolymers on earth, has been traditionally considered a low-value by-product of the pulp and paper industries. This renewable raw material, besides being a source of valuable molecules for the chemical industry, also has antioxidant, UV-absorbing, and antibacterial properties in its macromolecular form. Moreover, lignin in the form of nanoparticles (LigNPs) presents advantages over bulk lignin, such as higher reactivity due to its larger surface-to-volume ratio. In view of the rapid surge of antimicrobial resistance (AMR), caused by the overuse of antibiotics, continuous development of novel antibacterial agents is needed. The use of LigNPs as antibacterial agents is a suitable alternative to conventional antibiotics for topical application or chemical disinfectants for surfaces and packaging. Besides, their multiple and unspecific targets in the bacterial cell may prevent the emergence of AMR. This review summarizes the latest developments in antibacterial nano-formulated lignin, both in dispersion and embedded in materials. The following roles of lignin in the formulation of antibacterial NPs have been analyzed: (i) an antibacterial active in nanoformulations, (ii) a reducing and capping agent for antimicrobial metals, and (iii) a carrier of other antibacterial agents. Finally, the review covers the inclusion of LigNPs in films, fibers, hydrogels, and foams, for obtaining antibacterial lignin-based nanocomposites for a variety of applications, including food packaging, wound healing, and medical coatings.