Antimicrobial Activity Of Methanol Extracts Research Articles

Phytochemical Screening and Antimicrobial Activity of Stem Bark Extracts of Schinus molle linens

The majority of the world population prepares medicines from plants to cure diseases. Schinus molle linens is a multipurpose plant that has been used for various remedies as constituents of traditional medicine. The aim of this study was to investigate the chemical constituents and antimicrobial activity of methanol extract and solvent fraction extracts of the stem bark of Schinus molle. Powder of Schinus molle was extracted with methanol by using the maceration method and the fractions were obtained by successive fractionation of the methanol extract with n-hexane, chloroform, ethyl acetate, and n-butanol. Preliminary phytochemical analysis of the crude methanol extract revealed the presence of flavonoids, terpenes, anthraquinones, alkaloids, tannins, phenols, and saponins. In addition to this, the antimicrobial activity of crude and solvent fraction extract was tested against S. aureus, E. faecalis, E. coli, and C. albicans at a concentration of 90.0, 180.0, and 900.0 μg/ mL. The crude methanol extract and chloroform fraction exhibited greater activity against gram-positive, gram-negative bacteria and fungi. The n-hexane and n-butanol fractions showed the least activity against most test bacteria and fungi. The study showed that it is possible to inhibit the growth of bacteria and fungi at different concentrations.

In vitro Hypoglycemic Effect and Antimicrobial Activity of Methanol Extract of Underutilized Leafy Vegetable (Ipomoea batatas leaf)

Diabetes mellitus is a chronic condition characterized by major derangements in glucose metabolism and abnormalities in fat and protein metabolism. Diabetes and infection are among the serious health challenges. This study evaluated the in vitro and hypoglycemic effect and antimicrobial properties of methanol extract of Ipomoea batatas leaf. The antimicrobial activity was done using agar disc diffusion methods and the in vitro hypoglycemic activity was done using Alpha-Glucosidase Inhibitory activities assay, Lipase activity assay, Glucose absorption Capacity assay, Glucose diffusion retardation Index. The result of the percentage alpha glucosidase inhibition ranged from 35.70 to 56.33%. The percentage alpha amylase inhibition ranged from 40.96 to 65.15%. The extract showed significant (p<0.05) dose dependent inhibition of alpha glucosidase and alpha amylase. The extract possess inhibition of lipase activity with percentage inhibition ranging from 72.35 to 81.57%. Though there was non-significant (p<0.05) difference in the various concentrations of the extract on inhibition of lipase activity. The result of the glucose absorption capacity (0.30 to 2.25) was observed to be dose dependent. The glucose diffusion retardation index was time dependent. The antimicrobial susceptibility screening of extracts showed a zone of inhibition that ranged from 2.70 to 15.73mm. The study showed that Ipomoea batatas leaf could be used in the treatment and management of diabetes and infections.

Chemical Characterization and Several Bioactivities of Cladanthus mixtus from Morocco

The purpose of this work was to investigate, for the first time to our knowledge, the chemical composition and bioactivity of methanolic extracts (roots, stems, leaves, and flowers) from Cladanthus mixtus (L.) Chevall. that grows wild in northern Morocco (the Tangier-Tetouan-Al Hoceima region). The phenolic and flavonoid contents were determined by spectrophotometer methods, and the composition of derivatized methanolic extracts from C. mixtus using N-O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) was analyzed by gas chromatography-mass spectrometry (GC-MS). The antioxidant activity was carried out by applying the 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and DPPH (2,2-diphenyl-1-picrylhydrazyl) tests. The micro-dilution technique was chosen to investigate the antimicrobial activity of methanolic extracts against two bacterial strains and three fungal species. The results showed that the values of total phenolic and flavonoid contents were found to be higher in flower extracts (30.55 ± 0.85 mg of gallic acid equivalents (GAE)/g of dried weight (DW) and 26.00 ±1.34 mg of quercetin equivalents (QE)/g DW, respectively). Other groups of chemical compounds were revealed by GC-MS, such as carbohydrates (27.25-64.87%), fatty acids (1.58-9.08%), organic acids (11.81-18.82%), and amino acids (1.26-7.10%). Root and flower methanolic extracts showed the highest antioxidant activity using ABTS (39.49 mg of Trolox equivalents (TE)/g DW) and DPPH (36.23 mg TE/g DW), respectively. A positive correlation between antioxidant activity and polyphenol and flavonoid amounts was found. Antibacterial tests showed that the best activity was presented by the leaf extract against Staphylococcus aureus (minimum inhibitory concentration (MIC) = minimum bactericidal concentration (MBC) = 20 mg/mL) and Escherichia coli (MIC of 30 mg/mL and MBC of 35 mg/mL). S. aureus was more sensitive to the extracts compared to E. coli. All extracts showed antifungal activity against Trichophyton rubrum, with the best efficacy reported by the flower and leaf extracts (MIC = 1.25 mg/mL and minimum fungicidal concentration (MFC) = 2.5 mg/mL). In general, extracts of C. mixtus appeared less effective against Candida albicans and Aspergillus fumigatus.

Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Ranunculaceae) – a critically endangered endemic species growing in the Turkish flora

This study was conducted to determine the antioxidant and antimicrobial activities of methanol extract obtained from flower, leaf and root sections of endemic Adonis paryadrica (Boiss.) Kandemir & Aytaç stat. nova. naturally growing in the Turkish flora. The most efficient total phenolic compounds and flavonoid contents were obtained from leaf extract at 21.24 mg GAEs (gallic acid equivalent)/g dw and 54.97 mg REs (rutin equivalent)/g dw, respectively. Among the three different sections of this plant, leaf extracts showed the highest Cupric Reducing Antioxidant Power (CUPRAC) effect with 80.28 µmol TEs (trolox equivalent)/g dw. From the three different sections, the methanol extract of the leaf parts demonstrated strong antibacterial activity against Bacillus subtilis with a 16.1 mm zone diameter. These valuable and current findings from these precious plants, which constitute natural resources in terms of biodiversity, contribute innovative information to the literature on endemic plant species.

Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?

Abstract: The aim of this study is to determine the effect of growing media on antioxidant properties and antimicrobial activities of Pleurotus ostreatus(Jacq. ex Fr.) P. Kumm. In the study, growing substrates were prepared with five tree sawdust (80%) and waste (20% Tea Waste or Wheat Bran) at different ratios (80% Sawdust + 20% TW, 80% Sawdust + 20% GW). The antimicrobial activities of methanol extracts from 19 P. ostreatus against microorganisms were tested by agar well diffusion technique. The total phenolic content was measured using the Folin-Ciocalteau procedure. The antioxidant capacity was determined using ferric reducing antioxidant power (FRAP) and free radical scavenging activity of DPPH. It was observed that the addition of 20% tea waste in the substrate had a positive effect on antimicrobial activity. It was determined that the methanol extract of mushrooms obtained from the 80QS+20TW substrate was the most effective extract against to all of microorganisms investigated in this study. Significant differences (P

Evaluation of DNA Protective and Antimicrobial Properties of some Cladonia Species

The present study evaluated the DNA protective properties and antimicrobial activities of the methanol extracts of nine Cladonia species, namely C. pocillum, C. subulata, C. pyxidata, C. coniocraea, C. foliacea, C. firma, C. furcata, C. fimbriata and C. rangiformis collected in Turkey. DNA protection properties efficiency of Cladonia extracts was evaluated using pBR322 plasmid DNA. In vitro antimicrobial activities of methanol extracts against two Gram-negative bacteria (Escherichia coli and Proteus mirabilis), three Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis) and two fungal strains (Candida glabrata and Candida albicans) were examined using the disc diffusion method and through the determination of minimal inhibitory concentrations (MIC). DNA protective studies, all Cladonia extracts protected pBR322 plasmid DNA against damage caused by the hydrogen peroxide (H2O2) with ultraviolet (UV). The results demonstrated that the inhibition zones in the disc diffusion method ranged from 6.5 to 19.0 mm. MIC results were ranged from 3.12 to 6.25 mg/mL. Cladonia extracts show a better antimicrobial effect against bacterial strains than fungal strains. The highest antimicrobial effect among lichen species was demonstrated by Cladonia pocillum. Our results demonstrated that tested Cladonia extracts had strong antibacterial and DNA protective effects. This is the first comprehensive study to evaluate the DNA protective properties activity of Cladonia extracts.

Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Asteraceae) – a critically endangered endemic species growing in the Turkish flora

This study was conducted to determine the antioxidant and antimicrobial activities of methanol extract obtained from flower, leaf and root sections of endemic Adonis paryadrica (Boiss.) Kandemir & Aytaç stat. nova. naturally growing in the Turkish flora. The most efficient total phenolic compounds and flavonoid contents were obtained from leaf extract at 21.24 mg GAEs (gallic acid equivalent)/g dw and 54.97 mg REs (rutin equivalent)/g dw, respectively. Among the three different sections of this plant, leaf extracts showed the highest Cupric Reducing Antioxidant Power (CUPRAC) effect with 80.28 µmol TEs (trolox equivalent)/g dw. From the three different sections, the methanol extract of the leaf parts demonstrated strong antibacterial activity against Bacillus subtilis with a 16.1 mm zone diameter. These valuable and current findings from these precious plants, which constitute natural resources in terms of biodiversity, contribute innovative information to the literature on endemic plant species.

Investigation of Antimicrobial and Antioxidant Potential of Tinospora cordifolia by In-vitro Methods

Antimicrobial and Antioxidant Potential of Tinospora Cordifolia were performed by In-vitro Methods. For this study stem of plant Tinospora cordifolia where collected and dried under shade. Morphological characteristics and Physio-chemical parameters of Tinospora cordifolia stem were investigated. Plant materials were extracted by Soxhlet extraction method then Pharmacognostical evaluation of extract was subjected to various phytochemical tests for preliminary identification of various phytoconstituents. The free radicals scavenging potential of standard and extract tested by DPPH method are depicted in with a characteristic absorption at 517nm. Effects of methanolic extract of Tinospora cordifolia was evaluated against gram positive (S. aureus) and gram-negative (E. coli) bacteria and antifungal activity was obtained against Candida Albicans and Aspergilus Niger. Antimicrobial activities were estimated by disk diffusion method. Antimicrobial activities of methanolic extract of stem of tinospora were evaluated in the term of zone of inhibition. Methanolic steam extract of Tinospora cordifolia was significantly against both gram positive (Staphylococcus aureus) and gram-negative bacteria (E. coli). it was observed that the extract was not significantly effective against both gram positive and gram negative bacteria, while, Methanolic stem extract of Tinospora cordifolia was more effective against Candida Albicans and A niger.
 Keyword: Tinospora cordifolia, antioxidant, Antimicrobial,

Immunomodulatory and antimicrobial activities of methanol extract of Uvaria chamae leaves in rats

The immunomodulatory and antimicrobial effects of methanol extract air-dried, powdered leaves of methanol extract of Uvaria chamae (MEUL) was evaluated in this study using standard methods. Graded doses (50 mg/kg body weight, 100 mg/kg body weight, 200 mg/kg body weight and 400 mg/kg body weight) of the extract were administered to winster rats. The control group was given dimethyl sulphoxide (DMSO). Immunomodulatory effect was assessed by delayed type hypersensitivity (DTH) and haemagglutination. Antimicrobial activity of the extract against some clinical bacteria isolates was also conducted. The phytochemicals, alkaloids, flavonoids, glycosides, saponins, tannins, carbohydrates, steroids and terpenoids were detected. The extract showed dose related increase in cellular immunomodulatory activity as assesed by inflammed foot pad of the experimental animals with mean diameter ranging from 2.60±0.95 to 4.90±1.04 mm as against the control group which was 1.30±0.14 mm, (p<0.05). There was significant increase in haemagglutinating antibody titre against sheep red blood cells which ranged from 272±21.27 to 1344±160.64 as against the control group which was 80±10.63 (p<0.01). The methanol extract of Uvaria chamae leaf showed inhibitory activity against the test bacteria isolates. This work indicated that methanol extract of Uvaria chamae leaf has potential immunostimulatory and antimicrobial activity.

Antimicrobial Activity of Methanolic Extract from Clove and the Test of Preservative Power of Ras Cheese

Antimicrobial Activity of Methanolic Extract from Clove and the Test of Preservative Power of Ras Cheese

Antimcrobial activity of extract of Bryophyllum pinnatum and Citrus aurantifoliaon some selected pathogens.

The inhibitory properties of Bryophyllum pinnatum and lime juice on some selected microorganisms (Staphylococcus aureus,Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Aspergillus niger and Candida albicans) were investigated. The various concentrations of the extracts were prepared by double dilution method while the ability of the various extracts to inhibit the growth of the pathogenic organisms was determined using the agar well technique. The highest in-vitro antimicrobial activity of methanolic extract of Bryophyllum pinnatum was (13.0+2.83a mm at 200 mg/ml) against Klebsiella pneumoniae while the least antimicrobial activity was (10.5+2.12b and 10.0+0.00e mm at 200 mg/ml) against Streptococcus pyogenes and Candida albicans respectively. The lime juice extracts had the highest in-vitro antimicrobial activity was (11.0+0.00b mm at 200 mg/ml) against Staphylococcus aureus while the least antimicrobial activity was (7.0+ 0.0bmm at 200 mg/ml) against Streptococcus pyogenes. The methanol and aqueous soluble plant extracts were active against all the test isolates but methanol extract of Bryophyllum pinnatum demonstrated greater inhibitory activity (MIC) on Staphylococcus aureus, Escherichia coli, Streptococcus species and Aspergillus niger at the range of 12.5 mg/ml. The lime juice extracts demonstrated greater inhibitory activity (MIC) on Escherichia coli at 12.5 mg/ml. The results in the findings showed that the methanolic and aqueous extracts of Bryophyllum pinnatum as well as lime juice extracts have broad-spectrum antimicrobial activity and can serve as natural therapeutic agent against some enteric pathogens.

A Study of active medicinal plant (Artemisia Judaica) against Staphylococcus aureus

Staphylococcus aureus infection is a rather insidious disease. If tests have shown that Staphylococcus aureus is found on the skin, intestines, ears or nasopharynx, Treatment with pharmaceutical drugs extracted from medicinal plants can be a way to get rid of infection. Plants are considered the greatest source to obtain new antimicrobials. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of Artemisia Judaica plant against Staphylococcus aureus Sp. and others pathogenic bacteria, these bacteria are commonly found in hospital-acquired infections. The methanolic extract of Artemisia Judaica effective against the isolates microorganisms, S. aureus, E. coli, P. aeruginosa, The diameter of zone of inhibition was found to be in the range of 14 – 30 mm against various bacterial strains tested, with maximum diameter against bacteria (S. aureus, 30 mm). The methanolic extract of Artemisia Judaica presented the highest anti-staphylococcus aureus activity and was effective against others bacterial strains tested.

Comparative determination of antimicrobial activity of the Balkan endemic species Stachys thracica Davidov during the process of ex situ conservation

Stachys thracica Davidov – Thracian woundwort is a Balkan endemic plant included in The Red Data Book of Bulgaria with conservational status “rare”. The plants from genus Stachys have a long history of use to treat various diseases, inflammatory conditions, coughs, ulcers, genital tumors, and infected wounds. Due to its limited distribution the information on the biological activity and chemical composition of S. thracica is rather scarce. The aim of the present research is the comparative determination of the antimicrobial activity of methanolic extracts obtained from in situ wild, in vitro cultivated and ex vitro adapted S. thracica plants. The in vitro shoot culture of the Thracian woundwort was maintained in hormone-free MS medium under controlled environmental conditions. The methanolic extracts from in situ, in vitro cultivated and ex vitro adapted S. thracica plants were active mainly against Gram-negative bacteria. All three extracts showed equal activity against Acinetobacter calcoaceticus. The establishment of in vitro shoot culture and its subsequent adaptation in ex vitro conditions was an appropriate alternative approach for the ex situ conservation of S. thracica as well as for the study of its biological activity.

In vitro cytotoxicity and antimicrobial activity of methanolic extracts of Holothuria (Semperothuria) cinerascens (Brandt, 1835) and Stomopneustes variolaris (Lamarck, 1816) from the southwest coast of India

Marine biology,marine ecology,Phytoplankton, marine algae, zooplankton, nekton, benthos, corals and coral reefs, ecology, zoogeography and systematics, primary production and experimental work on ecosystem including energy flow at different trophic levels, population dynamics and theoretical models, recycling of matter, influence of environmental factors on metabolic activity, marine microbiology, mariculture and experimental work on propagation of cultivable species, biology of fishes, marine reptiles, sea birds and mammals, physical, chemical, geological and meteorological features of the environments related to marine biology, marine environmental damage caused by pollution and engineering works, underwater observations, conservation of living resources, modifications in methodology and techniques and development of new apparatus related to marine sciences

Evaluation of bioavailability of three extracts of a less known ethno-medicinal plant, Thottea tomentosa from Assam

Plants of the genus Thottea have been established as wild ethno-medicinal plants. The plant, Thottea tomentosa also has the illness recuperative property. However there are no such reports so far on the solvent polarity base performances of the plant. Hence, the current work deals with the assessment of bioavailabilities like qualitative phyto-chemical screening, antioxidant efficiency, total phenol contents, total flavonoid contents and antimicrobial activity using various solvents of diverse polarities like hexane, chloroform, and methanol. Qualitative phyto-chemical screening exposes the stem part contains more phyto-chemicals than that of the leaf. In addition, phenolic compositions of methanol extract of stem and leaf; 374.99 ± 3.84 and 260.55 ± 4.00 mg GAE /g extract respectively comprises with a correlation between antioxidant activities of methanol extract of the stem and leaf (DPPH; IC50 202.39 ± 0.92 and 254.37 ± 1.47 µg/ml respectively). Thus, probable antimicrobial activities of methanol extract of stem and leaf were also study, than that of other extracts. Methanol stem extract against the microbes; Bacillus subtilis (MTCC 1427), Escherichia coli (MTCC 1195), Pseudomonas aeruginosa (MTCC 1688), Staphylococcus aureus susp.aureus (MTCC 1430), Streptococcus pneumonie (MTCC 2672), Aspergillus niger (Lab isolates) and Candida albicans (MTCC 4748) exhibited highest zone of inhabitation (ZOI); 17.66 ± 1.45, 15.66 ± 0.88, 18.66 ± 0.66, 17.66 ± 1.45 19.33 ± 0.66 mm, 15.00 ± 1.15 and 14.66 ± 1.20 mm, respectively at concentration 20 mg/ml. Therefore, these results indicate that T. tomentosa can be satisfying the dietetic demand for the food and drug production.

Isolation of oleuropein from olive leaf by effective method and investigation of its antimicrobial properties

Natural products play an important role in the production and development of food and medicine. In olive tree cultivation and olive processing industry, vast amounts of by-products are yielded. Olive leaves constitute the major by-product of olive industry. Olive leaf is potential source of phenolic compounds. Plant polyphenols have attracted great interest due to their antimicrobial activities. It was aimed to isolate the oleuropein compound from olive leaf and to identify its antimicrobial effects in this research. In the first stage, methanol extract was obtained from the olive leaf. After then, ethyl acetate extract (including oleuropein with 93.5% purity) procured from methanol extract. Ethyl acetate extract was subjected to chromatographic technique to obtain oleuropein in high purity (97.6%). The structure of oleuropein was determined by spectroscopic method including 1H and 13C NMR besides comparing isolated oleuropein with the standard commercially obtained. Antimicrobial activity of methanol extract, ethyl acetate extract and oleuropein was investigated on Bacillus subtilis, Candida tropicalis, Lactobacillus brevis, Lactobacillus plantarum, Proteus vulgaris, Saccharomyces cerevisiae, Salmonella enteritidis microorganisms by 96-well microtiter plate method. It was determined that MIC (Minimum Inhibitory Concentration) values of olive leaf products ranged between 1:1 (50 mg/mL) and 1:8 (6.25 mg/mL) for tested microorganisms.

Investigation the Antimicrobial Activity of Methanol Extracts of the Leaves of Ficus Nitida

The aim of this study was to prepare crude extracts from the leaves of Ficus nitida and determine their content as well as their antimicrobial activity. Powdered leaf samples were extracted with methanol by using maceration method. The resulting crude extract was suspended in water and successively extracted with petroleum ether, chloroform, ethyl acetate and methanol solvents respectively. Total flavonoids of the above extracts were determined by the aluminum chloride method and antimicrobial activity of the same extracts was assessed by a slightly modified disc diffusion method against different gram positive and gram negative bacterial strains. The highest flavonoids content was found in chloroform crude extract followed by that of petroleum ether, ethyl acetate and methanol extracts respectively. Tested against four bacterial strains, the extracts showed activity in the range of 0-12 mm, using amoxicillin as standard. The present findings could establish this plant as a potential source of antibiotics.

Phenolic Compound Content and the Antioxidant and Antimicrobial Activity of Wild Blueberries (Vaccinium stenophyllum Steud.) Fruits Extracts during Ripening

The genus Vaccinium contains about 400 species distributed worldwide, but only a few species and varieties have had their phenolic composition and biological activity documented. In this study, phenols, flavonoids, anthocyanins, antioxidant and antimicrobial activity of methanolic extracts of Vaccinium stenophyllum Steud. fruits: 1-totally immature, 2-immature, 3-immature/close to maturity and 4-mature, were determined using UV-Visible spectrometry and HPTLC. The totally immature fruit extract showed the highest content of total phenols (19.153 ± 0.175 mg GAE/g DW), chlorogenic acid (20.867 ± 0.240 mg CAE/g DW), and the highest antioxidant activity by ABTS●+ (196.761 ± 0.641 µM TE/g DW) and DPPH● (146.580 ± 6.466 µM TE/g DW). Immature, immature/close to maturity and mature fruits extracts, exhibited the lowest MIC (9.37 mg/mL) and MBC (18.75 mg/mL) against Escherichia coli, Salmonella choleraesuis, and Shigella flexneri. The mature fruits extract exhibited the highest content of total anthocyanins (0.141 ± 0.004 mg CE/g DW) and cyanidin-3-glucoside (19.230 ± 0.309 mg CGE/g DW). The content of phenols, flavonoids and anthocyanins was higher than that reported for other Vaccinium species. These results showed the relevance of Vaccinium stenophyllum Steud. for breeding purposes to enhance the bioactivity of cultivars, or as a source of natural additives for the food industry, among others.

Phytochemical and proximate content of Carapa procera bark and its antimicrobial potential against selected pathogens.

Medicinal plants represent a great source of antimicrobial and phytochemical constituents which are increasingly used to treat microbial infections and other ailments such as tuberculosis, anemia, and trachoma. Despite the use of antibiotics, antimicrobial resistance continues to be a world issue, in as much as nutrition. This study investigated the presence of phytochemicals, proximate compositions, and antimicrobial activity of methanolic extract of Carapa procera bark. The bark of Carapa procera was collected, cleaned and air dried for 72 h. The powder obtained was treated with diethyl ether and soaked in methanol (99%) for 72 h to obtain crude extract. The extract was used to test for the presence of phytochemicals and antimicrobial activities. The raw bark was used for proximate analysis. The result showed presence of steroids, tannins and saponins, but no alkaloids present. The 100 mg/mL extract had the highest inhibition zone on all tested organisms from 24.00 ± 0.94 to 26.67 ± 1.18, and 50 mg/mL showed the least (16.67 ± 1.24) on Candida albicans. Staphylococcus aureus showed the lowest minimum inhibition concentration (MIC) of 3.12 mg/mL, whereas the Gram-negative bacteria exhibited variations in their sensitivity with E. coli having the highest MIC of 25 mg/mL. The extract had high MIC (6.25 mg/mL) on Candida albicans than clotrimazole (50 mg/mL). The proximate compositions of Carapa procera were moisture (6.07 ± 0.07%), ash (12.46 ± 0.46%), crude protein (9.54 ± 0.12%), crude fat (1.42 ± 0.06%) and carbohydrate (70.50 ± 0.35%). The energy value was 1413.17 kj. Thus, Carapa procera possesses both antimicrobial and nutritional potentials worth exploring and domesticating for sustainable management and conservation.

Phytochemical screening and evaluation of antimicrobial activity of Albizia ferruginea (Fabaceae) leaves extract

Phytochemical and antimicrobial activity of methanol extract of Albizia ferruginea (Guill and Perr) which belongs to the family of Fabaceae were studied. Phytochemical studies on the plant leaves showed that they contained alkaloids, tannins, flavonoids, steroids, saponins, cardiac glycoside and carbohydrate. Methanol extract of Albizia ferruginea showed growth inhibitory effects of varying degrees on S. feacalis, S. typhi, S. paratyphi, Shigella dysentriae and Klebsiella pneumonia but was not active on Streptococcus pyogenes and Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) of the extract was determined for the organisms whose growths were inhibited. The extract had MIC of 3.25mg/ml for S. feacalis, S. paratyphi, S. dysentriae and K. pneumoniae respectively. The antimicrobial studies of the extracts really justify the numerous folkloric uses of the plant parts in the treatment of some common ailments such as diarrhea, dysentery, fever, cough, pain and skin infections.