Antihistaminic Properties Research Articles

Cetirizine platinum(IV) complexes with antihistamine properties inhibit tumor metastasis by suppressing angiogenesis and boosting immunity

The histamine (HA) in tumors plays critical roles in promoting metastasis. Herein, a series of cetirizine (CTZ) platinum(IV) complexes with antihistamine properties were developed as antimetastatic agents. Dual CTZ platinum(IV) complex with cisplatin core was screened out as a candidate displaying potent antiproliferative activities. More importantly, it exerted promising antimetastatic properties both in vitro and in vivo. Investigation of the mechanism revealed that serious DNA damage was induced, which further led to the upregulation of histone H2AX (γ-H2AX) and P53. The mitochondria-mediated apoptosis was ignited through the B-cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax)/caspase3 pathway. Moreover, the HA-histamine receptor H1 (HRH1) axis was inhibited, then the key signaling phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) was suppressed. Subsequently, the angiogenesis in tumors was restrained by suppressing the inflammatory and hypoxic microenvironment. Then, the antitumor immunity was reinforced by increasing the CD3+ and CD8+ T cells and promoting the polarization of macrophages from M2- to M1-type, which was associated with the blockade of programmed cell death ligand-1 (PD-L1) expression in tumors.

Antihistamines H1 as Potential Anthelmintic Agents against the Zoonotic Parasite Angiostrongylus cantonensis.

Infections caused by parasitic helminths pose significant health concerns for both humans and animals. The limited efficacy of existing drugs underscores the urgent need for novel anthelmintic agents. Given the reported potential of antihistamines against various parasites, including worms, this study conducted a screening of clinically available antihistamines against Angiostrongylus cantonensis-a nematode with widespread implications for vertebrate hosts, including humans. Twenty-one anti-H1 antihistamines were screened against first-stage larvae (L1) of A. cantonensis obtained from the feces of infected rats. Standard anthelmintic drugs ivermectin and albendazole were employed for comparative analysis. The findings revealed four active compounds (promethazine, cinnarizine, desloratadine, and rupatadine), with promethazine demonstrating the highest potency (EC50 = 31.6 μM). Additionally, morphological analysis showed that antihistamines induced significant changes in larvae. To understand the mechanism of action, antimuscarinic activities were reported based on average pK i values for human muscarinic receptor (mAChR) subtypes of the evaluated compounds. Furthermore, an analysis of the physicochemical and pharmacodynamic properties of antihistamines revealed that their anthelmintic activity does not correlate with their activity at H1 receptors. This study marks the first documentation of antihistamines' activity against A. cantonensis, offering a valuable contribution to the quest for novel agents effective against zoonotic helminths.

Orexinergic Receptor Antagonists as a New Therapeutic Target to Overcome Limitations of Current Pharmacological Treatment of Insomnia Disorder.

Insomnia disorder is a common condition that is considered a risk factor for multiple physical and mental disorders, contributing to reduced quality of life and increased healthcare expenditures. Although cognitive behavioral therapy (CBT) is typically recommended as the primary intervention, its accessibility is hindered by limited resources, prompting the prevalent use of pharmacological interventions as the primary treatment in clinical settings. This study reviews the benefits and risks of current pharmacological treatments for insomnia, with special reference to the orexinergic system as a novel therapeutic target for treatment. The prescription of GABAergic mechanism enhancers (benzodiazepine (BZD) and "Z drugs") has shown efficacy in short-term insomnia treatment (less than 4 weeks), however, concerns arise regarding their long-term effectiveness, unfavorable tolerability and safety profiles, including the potential for dependency. Drugs with antihistamine properties, including certain antidepressants and antipsychotics, exhibit short-term efficacy but have documented tolerability limitations, especially in the elderly. The use of melatonin, available in various formulations, lacks comprehensive long-term data. Dual orexin receptor antagonists (DORAs) such as daridorexant, lemborexant, and suvorexant, represent a novel approach to insomnia treatment by inhibiting wakefulness rather than enhancing sedation. As the only DORA approved for insomnia treatment by the European Medicines Agency (EMA) and Food and Drug Administration (FDA), daridorexant has demonstrated sustained efficacy over a 12-month period, improving nocturnal sleep parameters and daytime functionality, with a favorable safety and tolerability profile.

The investigation of hawthorn (Crataegus orientalis) plant’s inhibition effect on angiotensin converting enzyme and in silico studies

Hawthorn plant is used among people due to its cardiovascular, anti-inflammatory, and antihistamine properties. But no scientific study has been done about Crataegus orientalis (Mill.) M.Bieb. The presented study was planned to determine the effects of ethanol and n-hexane extracts of Crataegus orientalis leaves on human plasma ACE enzyme. In the study, the effect of plant extracts on ACE was studied by the spectrophotometric method. The chemical composition of the plant extracts was determined by HPLC-DAD analyses. In addition, molecular doking and ADME prediction studies were carried out. As a result, the obtained data showed that Crataegus orientalis could have an important place in the pharmaceutical industry and drug discovery studies, as it supports the traditional use of Crataegus orientalis as hypotensive. The results of the molecular docking studies revealed that the interactions of the selected compounds with the human ACE enzyme caused inhibition.

Effect of Clemastine on Neurophysiological Outcomes in an Ovine Model of Neonatal Hypoxic-Ischemic Encephalopathy.

Originally approved by the U.S. Food and Drug Administration (FDA) for its antihistamine properties, clemastine can also promote white matter integrity and has shown promise in the treatment of demyelinating diseases such as multiple sclerosis. Here, we conducted an in-depth analysis of the feasibility, safety, and neuroprotective efficacy of clemastine administration in near-term lambs (n = 25, 141-143 days) following a global ischemic insult induced via an umbilical cord occlusion (UCO) model. Lambs were randomly assigned to receive clemastine or placebo postnatally, and outcomes were assessed over a six-day period. Clemastine administration was well tolerated. While treated lambs demonstrated improvements in inflammatory scores, their neurodevelopmental outcomes were unchanged.

IDENTIFICATION OF ANTI-ASTHMATIC DRUG FROM MEDICINAL PLANTS USING AN IN SILICO APPROACH

Objectives: Considering the limitations and side effects of current synthetic medications, herein, the exploration of the anti-inflammatory and antihistamine properties of medicinal plants is conducted to provide alternative treatment options for asthma and aims to identify potential anti-asthmatic drugs using an in silico approach. Methods: A molecular docking study was performed to assess the binding affinities and interactions between the neuropeptide S receptor (NPSR) protein and 15 medicinal plants and flavonoids chosen from published literature. A Ramachandran Plot analysis was conducted to evaluate the stereochemical properties of the protein. Furthermore, to gain insights into the drug-likeness and pharmacokinetic properties of the identified ligands, ADMESAR analysis was performed to predict molecular properties and bioactivity of small molecules. Results: Among the 15 medicinal plants investigated in this research, Kaempferol exhibited the least binding energy of −5.05, indicating a highly stable interaction with the NPSR protein. This exceptional stability suggests that Kaempferol has the potential to serve as an effective anti-asthmatic drug. Conclusion: Asthma has no permanent cure, and the current synthetic medications raise long-term safety concerns. This study explored the use of medicinal plants and flavonoids, with Kaempferol showing promise as a potential anti-asthmatic drug candidate. This preliminary study could open avenues to further research and the use of medicinal plants in the treatment of asthma, potentially reducing reliance on synthetic drugs.

Antidepressants

Tricyclic antidepressants possess anticholinergic, alpha 1 anti-adrenergic, and H1 antihistaminic properties, and an overdose affects patients' quality of life, which has led to the development of novel antidepressant drugs. Selective serotonin reuptake inhibitors (SSRIs) are non-sedating drugs that selectively reuptake serotonin and are effective against anxiety. Adverse effects of SSRIs include gastrointestinal disturbances, sexual dysfunction, and bleeding tendency. Serotonin noradrenaline reuptake inhibitors (SNRIs) are non-sedating agents that are expected to improve volition. SNRIs are effective against chronic pain, although they are associated with gastrointestinal symptoms, tachycardia, and elevated blood pressure. Mirtazapine is a sedative drug used in patients with anorexia and insomnia. However, drowsiness and weight gain are known adverse effects of this medication. Vortioxetine is a non-sedative drug associated with gastrointestinal symptoms; however, insomnia and sexual dysfunction are less common.

Comparison of the Anti-asthmatic Potential of Himalayan Plants Lonicera obovata Royle and Morina longifolia Wall: An in vivo Study

Pharmacognosy Research,2023,15,2,384-391.DOI:10.5530/pres.15.2.041Published:February 2023Type:Original Article Authors:Pooja Boora, Richa Puri, Sushila Rani, Monika Mehta, and Dechan Angmo Author(s) affiliations:Pooja Boora*, Richa Puri, Sushila Rani, Monika Mehta, Dechan Angmo Department of Botany, Panjab University, Chandigarh, INDIA. Abstract:Background: Asthma is a very common and chronic disease in which there is an inflammation of the lungs. Inflammatory airways and narrowing, spasms, over-secretion of mucus, bronchoconstriction, and eosinophilic airway inflammation are generally seen in the asthmatic condition. Globally, its prevalence is rising constantly. The extracts of Lonicera obovata and Morina longifolia of the Caprifoliaceae family were traditionally used by the Himalayan local people to treat asthmatic and inflammatory conditions. Objectives: The goal of this experimental study was to identify the phytochemicals of the plants through GC-MS. To investigate and compare the anti-asthmatic potential of methanolic whole plant extracts of L. obovata and methanolic root extracts of M. longifolia through in-vivo experiments. Materials and Methods: The anti-asthmatic potential of extracts was determined by the method of leukocytosis and eosinophilia induced by milk in the Swiss albino mice model. Results: Various secondary active phytoconstituents were detected through the GC-MS screening method, some of which have anti-allergic (anti-asthmatic) and antihistaminic properties. The in vivo experiment result showed that in the case of increased leukocyte count, M. longifolia shows better results than L. obovata, and in the case of increased eosinophil count, L. obovata shows better results than M. longifolia to reduce the increased count. Conclusion: The anti-asthmatic potential of plant extracts could be due to the presence of the phytoconstituents like 1-Monolinoleoylglycerol trimethylsilyl ether in the L. obovata extracts and Octadec-9-enoic acid and Cis-vaccenic acid in both L. obovata and M. longifolia extracts. Hence, through the experiments, the unexplored anti-asthmatic properties were revealed and justifies the traditional usage of both plants to treat asthma. Keywords:Anti-asthmatic, Eosinophilia, in vivo, leukocytosis, Lonicera obovata, Morina longifolia.View:PDF (366.66 KB)

Intrahepatic cholestasis of pregnancy: Green-top Guideline No. 43 June 2022.

Intrahepatic cholestasis of pregnancy: Green-top Guideline No. 43 June 2022.

Antihistaminic effects of Azadirachta indica leaves in laboratory animals

Azadirachta indica (Meliaceae) leaves have been traditionally used in the management of asthma and the current study was undertaken to scientifically validate the benefits of plant as an antihistaminic agent using the suitable animal model. The agents with antihistaminic properties are known to be good antiasthmatic agents; hence, in the current research work, the antihistaminic activity of an ethanolic extract of Azadirachta indica leaves (at a dose of 250 mg/kg, i.p.) was evaluated using haloperidol-induced catalepsy and clonidine-induced catalepsy in laboratory rats. The results showed that the ethanolic extract inhibits the catalepsy induced by the clonidine but no remarkable effect was observed on the catalepsy induced by haloperidol. This strongly suggests that, the inhibition is mediated through an antihistaminic action and there is no role of dopamine. Hence, in the present study, it is concluded that, the ethanolic extract has significant antihistaminic activity. The polar constituents in the ethanolic extract of leaves of Azadirachta indica may be responsible for the antihistaminic effects and therefore, the ethanolic leaves extract can be a better remedy as an antihistaminic agent.

Quetiapine Addiction: A Case Report

IntroductionQuetiapine has been the subject of case reports documenting its abuse. In Morocco, no study has been done showing the prevalence of this misuse. The methods of administration are diverse: oral or nasal, injection, inhalation, consumption with cannabis (smoked) or alcohol, combination with other drugs. The abuse is associated in 75% of cases with another product.ObjectivesThe objective of this work is to describe the management of quetiapine dependence, through a clinical vignette.MethodsThrough a clinical vignette, and by reviewing the literature, we will describe the management of quetiapine addiction.ResultsTreatment consists of reducing the consumption of this substance until stopping. When possible, it is recommended to change this antipsychotic to another with low abuse potential and low antihistamine properties such as haloperidol, risperidone or aripiprazole. If, however, this solution was inapplicable, then limit the quantity of tablets by prescribing smaller amounts of antipsychotics and increase the frequency of visits.Cope and relieve:Sometimes other medicines can be used to relieve potential withdrawal symptoms, including benzodiazepines or hypnotics to manage insomnia.Warnings :Ideally, the drug should be reduced gradually with a gradual and planned decrease in the dose taken over the months.There should also be periodic evaluations.Long term treatment:Management must be biopsychosocial.Treating comorbidities is a fundamental step in preventing relapse.ConclusionsIt is a “prescription” use disorder! Each prescription should be carefully weighed and time bound. It seems important to be vigilant with regard to the dosages administered and the treatment regimens offered to the patients.DisclosureNo significant relationships.

Phytochemical analysis and anti-allergic activity of a combined herbal medicine based on bur-marigold, calendula and hawthorn

Using an experimentally selected extractant for balanced extraction of flavonoids, polysaccharides and polyphenols from bur-marigold herb, calendula flowers and hawthorn leaves and flowers, 3 suitable dry extracts and 1 combined extract in the selected ratio of raw materials were obtained. It was concluded that it was reasonable to standardize the combined herbal extract on the content of flavonoids in terms of luteolin-7-glucoside and content of polyphenols in terms of pyrogallol by UV method and the content of terpenoid compounds, in terms of oleanolic acid by densitometric method. The combined extract at a dose of 30 mg/kg in the model of anaphylactic shock has showed antiallergic activity at the level of tesalin and exceeds diazoline; the ability of the combined extract to reduce the permeability of skin capillaries at the level of the tesalin has established on the model of active cutaneous anaphylaxis and it significantly exceeded diazoline; the combined extract did not differ in membrane-stabilizing properties from the effect of desloratadine and was significantly superior to the reference drugs diazolin and tesalin in the test of indirect degranulation of mast cells. As a result, it was concluded that the mechanism of anti-allergic action of the combined extract is probably associated not only with antihistamine properties but also with the ability to stabilize cell membranes.

A Comparative Study of Fluticasone Alone and Fluticasone with Azelastine Nasal Spray in Patients with Allergic Rhinitis

In India, 20-30% of the population suffers from allergic rhinitis. Its prevalence increasing over the past many years and often adversely affects the quality of life. Little importance is given to allergic rhinitis and patients fail to correlate the ill-health to symptoms of allergic rhinitis in India. Studies have shown that patients with allergic rhinitis adversely affect the behavior, work performance, and lifestyle of patients. Hence this study was undertaken to compare the efficacy of fluticasone alone and in combination with azelastine. A total of 60 patients were randomly assigned into two groups (30 patients each), where the first group received fluticasone and the other group received fluticasone + azelastine. Drugs were administered as nasal spray and improvement were assessed using Total Nasal Symptom Score (TNSS) and Quality of life was assessed using Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) at baseline and at 2 weeks. Descriptive statistics and student’s t-test was used to analyze data. Both the groups had statistical improvement in TNSS and RQLQ scores when compared to baseline within the groups (p < 0.0001). Comparison of overall TNSS between the groups was statistically significant (p < 0.001), though total RQLQ scores were insignificant (< 0.02) when compared between two groups. It was observed that both drugs were safe and efficacious. By these results, we conclude that the combination therapy showed better improvement in TNSS when compared to fluticasone alone. Azelastine due to antihistaminic properties and fluticasone anti-inflammatory effect showed synergistic anti-inflammatory effect and improvement in quality of life

Natural Anti-inflammatory and Anti-allergy Agents: Herbs and Botanical Ingredients.

Allergies have been known to be an abnormally vigorous immune response in which the immune system fights off an allergen or antigen, initiating mast cells to release histamine into the blood. Substances that prevent mast cells from releasing histamine are considered antiallergic agents. The drugs utilized to treat allergy are mast cell stabilizers, steroids, anti-histamine, leukotriene receptor antagonists, and decongestants. Anti-histamine drugs have side effects such as drowsiness, confusion, constipation, difficulty urinating, blurred vision, etc. The use of medicinal plants for the effective and safe management of diseases has recently received much attention. Various herbs are utilized for their antiallergic and anti-histaminic properties. Some of the herbs useful in the management of allergic diseases of the respiratory tract, like Piper longum, Ocimum tenuiflorum, Solanum xanthocarpum have been discussed. Ample scientific evidence is available for the anti-histaminic and antiallergic activity of Azadirachta indica, Aloe vera, Tinospora cordifolia, and many other such herbs are safer to use as antiallergic agents have been reported. The review summarizes a wide variety of herbs and botanical ingredients with their common scientific names and distribution for easy identification and usage as safe antiallergic agents and discusses their molecular mechanisms involved in combating allergic reactions.

Molecular Determinants of the Kinetic Binding Properties of Antihistamines at the Histamine H1 Receptors.

The binding affinity of ligands for their receptors is determined by their kinetic and thermodynamic binding properties. Kinetic analyses of the rate constants of association and dissociation (kon and koff, respectively) of antihistamines have suggested that second-generation antihistamines have a long duration of action owing to the long residence time (1/koff) at the H1 receptors. In this study, we examined the relationship between the kinetic and thermodynamic binding properties of antihistamines, followed by an evaluation of the structural determinants responsible for their kinetic binding properties using quantitative structure–activity relationship (QSAR) analyses. We found that whereas the binding enthalpy and entropy might contribute to the increase and decrease, respectively, in the koff values, there was no significant relationship with the kon values. QSAR analyses indicated that kon and koff values could be determined by the descriptors FASA_H (water-accessible surface area of all hydrophobic atoms divided by total water-accessible surface area) and vsurf_CW2 (a 3D molecular field descriptor weighted by capacity factor 2, the ratio of the hydrophilic surface to the total molecular surface), respectively. These findings provide further insight into the mechanisms by which the kinetic binding properties of antihistamines are regulated by their thermodynamic binding forces and physicochemical properties.

Examination the Quality of Oil Obtained from Cornelian Cherry (Cornus mas L.) Seeds as an Additive in the Production of Cosmetic Preparations and Food Supplements

From ancient times the natural plant Cornelian cherry is used for various purposes. The healing properties of Cornelian cherry suit the human body and give it the necessary vitamins, acids, and everything else it needs for the body to function normally and healthily. Due to its antioxidant, antiallergic, antimicrobial, and antihistamine properties, it is increasingly used as a dietary supplement, as well as for medical and pharmaceutical purposes. In addition to the fruit of the Cornelian cherry, in the past, the oil of Cornelian cherry seeds was used, the content of which can be up to 30%. However, the data available in the literature are scanty and do not show true values because the oil content depends on many factors, such as the geographical origin of the Cornelian cherry, the harvest period, varieties, etc., which also affects the oil content in the seeds.
 Therefore, the aim of this study is to determine the average oil content of Cornelian cherry seeds, and to determine the obtained oil physico-chemical parameters that show the quality of the oil, namely oil viscosity, iodine value, peroxide value, acid value, and saponification value. Based on the obtained results, more information is clearly given about the quality of the obtained oil, as well as its use in the production of cosmetic preparations.
 Based on the conducted analyzes, it was shown that the oil obtained from the Cornelian cherry seeds was high quality, and that it was analyzed in its fatty acid composition similar to other vegetable oils such as sunflower oil, pumpkin oil, corn oil. The low of the peroxide value showed that the oil used has good resistance to oxidative spoilage, which is attributed to the composition of fatty acids and the presence of oil components that have a pronounced antioxidant effect, while the iodine value indicates that it is oil rich in saturated fatty acids such as palmitic, stearic and arachid, etc. where genotype plays an important role. The saponification value showed that these are fatty acids present in the triacylglycerols of this oil, which are low molecular weight, ie there are fewer of those with a larger number of C atoms. All obtained values ​​of the analyzed physical and chemical parameters are in accordance with the requirements imposed by the Regulations on edible vegetable oils (Official Gazette of the Federation Bosnia and Herzegovina No.21/11.), and as such can be used for cosmetic purposes.

EFFECT OF $STEVIA$ $REBAUDIANA$ ON MORPHO-FUNCTIONAL INDICATORS OF PERIPHERAL BLOOD

Rich in natural antioxidants, Stevia rebaudiana has immunoregulatory, anti-stress, anti-inflammatory, antiviral, antihistaminic properties, increases the body's nonspecific defense, bioenergetic level, and improves tissue respiration. We have studied the long-term effect of S. rebaudiana on morphofunctional parameters of peripheral blood.

Beneficial effects of polyherbal formulation (Bronco-T) on formaldehyde-induced lung toxicity in male Wistar rats.

Polyherbal compound (Bronco-T) has been extensively used as a traditional medicine for various therapies. However, very few report studies on anti-inflammatory and lung regeneration properties are evidenced. In the present study, we evaluated the beneficial actions and anti-inflammatory properties of polyherbal medicine, Bronco-T, exhibited by treating the lungs of rats exposed to formaldehyde to evaluate the beneficial properties. For this study, we divided into five groups': i.e. Group-I served as a control and the other four groups such as II, III, IV, and V are experimental. All animals maintained by regular feed and water ad libitum during the study. Formaldehyde vapors exposure at a single period of time (1hour) daily (40%formaldehyde at room temperature) for 21days period exposed all groups. The Bronco-T extracts about 50mg/kg BW administered to experimental groups and group IV rats treated with 500μ grams/Kg BW salbutamol. To understand the impact of formaldehyde exposure on the beneficial effects of Bronco-T, we evaluated hematological parameters, bronchoalveolar lavage (BAL), histamine levels, and histological alterations of lung architecture. Formaldehyde-induced adverse effects in lung and increased histamine levels in BAL compared to Bronco-T-treated rats act as a preventive immunological role in blood toxicity and recovery of lung architecture in Bronco-T-treated rats. This study showed the evaluation of antihistamine levels through HPLC analysis. Bronco-T has antioxidant and anti-histamine properties as the widest therapeutic window, and we continue to evaluate the pharmacological evaluations needed in our further studies.

Examination of Physicochemical Parameters of Products Based on Cornelian Cherry (Cornus mas L.) and Honey

The natural plant Cornus mas, sometimes called Cornelian cherry, has been known since ancient times and the fruit is used for various purposes. Cornus mas gives healthy fruits without the use of chemical protection, which are used as healthy, tasty and medicinal food. Cornus mas fruits can be used for eating fresh or in the form of numerous processed products: marmalade, jam, sweet, compote, syrup, juice, fruit yogurt, liqueur, wine and brandy. Due to its antioxidant, antiallergic, antimicrobial and antihistamine properties, it is increasingly used as a dietary supplement, as well as for medical purposes. Taking into account all the above, the aim of this paper is to examine samples of a mixture of honey and fresh wild Cornelian cherry, as well as a sample of honey and Cornelian cherry and determine the physical - chemical parameters: electrical conductivity, pH value, vitamin C content, HMF (hydroxy methyl furfural), as well as individual heavy metals such as iron, cadmium, lead, zinc, copper. Based on the obtained results, appropriate conclusions will be given and the possibility of registering these products as food supplements will be determined. Based on the performed analyzes, it was shown that the sample of honey and Cornelian cherry mixture had the highest pH value and electrical conductivity, and that the honey sample had the lowest value. Also, the highest value of water activity had the sample of a mixture of honey and Cornelian cherry, while the highest content of hydroxymethylfurfural had the content of Cornelian cherry. The analysis showed the absence of manganese (Mn), cadmium (Cd) and lead (Pb) in all samples, while it was shown that the highest content of Cu and Zn has the sample of Cornelian cherry 2.665 mg/kg Cu ​​and 14.41 mg/kg Zn, followed by a mixture of Cornelian cherry and honey Cu 2.778 mg/kg, Zn 14.670 mg/kg, while the honey sample has the highest Fe content of 16.72 mg/kg. This shows that the samples are rich in zinc, iron and copper, and that they are a good source for those minerals that could meet daily needs.

ANTI-ULCER ACTIVITY OF HYDROALCOHOLIC EXTRACT OF PIPER BETLE LEAF ON EXPERIMENTAL ANIMALS

Objective: The main objective of this study is to establish the anti-ulcer activity of hydroalcoholic extract of Piper betle leaf on experimental animals based on previously existing aspects such as antioxidant, antihistaminic, and antimicrobial properties of P. betle leaf.
 Methods: The leaves were collected, shed dried, and extracted by the Soxhlet apparatus using 70% ethanol. The anti-ulcer activity of the extract was evaluated in albino Wistar rats employing pyloric ligation and stress-induced antiulcer models. Ranitidine was served as a standard drug in both the models. The significance of activity was assessed using a one-way analysis of variance followed by Dunnett’s post-parametric test.
 Results: In the pyloric ligation model, the untreated control has shown 4.3 mEq/l of acidity, whereas the ranitidine-treated standard group shown 2 mEq/l and P. betle has shown 2.5 mEq/l acidity, respectively. In the stress-induced antiulcer model, the activity was more prominent, in the untreated control, there was 26 number of sores present, whereas the standard group showed only one number of ulcer sore, and in the P. betle treated group, there was four number of ulcer sores present.
 Conclusion: In the present study, P. betle exhibited potent antiulcer potential while compared with the untreated control and the activity is comparable with standard ranitidine. From the above findings, it can correlate the use of betel leaf as a digesting or gastroprotective agent.