Phytochemical analysis and anti-allergic activity of a combined herbal medicine based on bur-marigold, calendula and hawthorn

Abstract

Using an experimentally selected extractant for balanced extraction of flavonoids, polysaccharides and polyphenols from bur-marigold herb, calendula flowers and hawthorn leaves and flowers, 3 suitable dry extracts and 1 combined extract in the selected ratio of raw materials were obtained. It was concluded that it was reasonable to standardize the combined herbal extract on the content of flavonoids in terms of luteolin-7-glucoside and content of polyphenols in terms of pyrogallol by UV method and the content of terpenoid compounds, in terms of oleanolic acid by densitometric method. The combined extract at a dose of 30 mg/kg in the model of anaphylactic shock has showed antiallergic activity at the level of tesalin and exceeds diazoline; the ability of the combined extract to reduce the permeability of skin capillaries at the level of the tesalin has established on the model of active cutaneous anaphylaxis and it significantly exceeded diazoline; the combined extract did not differ in membrane-stabilizing properties from the effect of desloratadine and was significantly superior to the reference drugs diazolin and tesalin in the test of indirect degranulation of mast cells. As a result, it was concluded that the mechanism of anti-allergic action of the combined extract is probably associated not only with antihistamine properties but also with the ability to stabilize cell membranes.