Antiglobulin Consumption Test Research Articles

The direct antiglobulin consumption test with leukocytes and blood platelets from some patients with idiopathic pancytopenias

RésuméLe test direct de consommation de l'antiglobuline sur leucocytes et sur plaquettes a 6th regulierement trouvk positif dans un cas de pancytopdnie chronique idiopathique.Ces tests ont également été trouvés positifs chez un certain nombre de malades leucopéniques et thrombopéniques.The direct antiglobulin consumption test was found to be regularly positive with the leucocytes and platelets of a case of chronic idio‐pathic pancytopenia.These tests were also positive in a number of patients with leuco‐penia and thrombopenia.ZusammenfassungIn einem Fall von idiopathischer chronischer Pancytopenie war der direkte Antiglobulinkonsumptionstest mit den Leukozyten und Thrombozyten regelmäßig positiv. Bei weiteren Patienten mit Leukopenie und Thrombopenie wurden ebenfalls positive Antiglobulin‐konsumptionsreaktionen beobachtet.

Report of an elution method for tissue auto-antibodies based on human antiglobulin consumption test

SummaryAn elution technique based on the antiglobulin consumption test is described. The optimal conditions for the elution were determined. The elution method is used to corroborate results obtained with the antiglobulin consumption test in the search for tissue autoantibodies. The method can also serve to determine the nature and properties of the eluted globulins.RésuméDescription d'une technique d'élution basée sur le test de consommation d'antiglobuline. Les conditions optimales pour l'élution ont été définies. Cette méthode aide à confirmer les résultats obtenus par le test de consommation d'antiglobuline dans la recherche des autoanticorps antitissulaires. Les éluats obtenus peuvent servir à déterminer la nature et les propriétés des globulines spécifiques qu'ils contiennent.ZusammenfassungIm Anschluß an die AHG‐Konsumptionsmethode wird ein Absprengungsversuch beschrieben. Die Bedingungen für die günstigste Absprengungsmöglichkeit wurden untersucht. Mit Hilfe dieses Absprengungsversuches wird eine Verstärkung der Resultate der AHG‐Konsumptionsrnethode beim Nachweis von Globulinsubstanzen mit Gewebs‐Autoantikörpereigenschaften erzielt. Durch die Absprengung kann Ausgangsmaterial fur Untersuchungen über Natur und Eigenschaften der auftretenden Globuliasubstanzen gewonnen werden.

New Serological Methods for the Detection of the L.E. Factor

In 1955 Miescher succeeded in replacing the morphological method for the detection of the L.E.-factor (L.E.-phenomenon) by a serological one. The antiglobulin-consumption test proved quite useful for this purpose if the “antigen” was applied in the form of isolated cellular nuclei or lyophilized intact leucocytes. This method not only was more sensitive as compared to the L.E.-cell detection method but also made it possible to differentiate serologically the so-called pseudo-L.-E.-phenomena from the true L.E.-phenomenon. Subsequently we were able to demonstrate that human cellular nuclei could be replaced by nuclei from the pig, the sheep, the abbit, the guinea pig, the rat and the trout with the same result. In adsorption tests it was then found that the L.E.-factor was easily fixed by purified nucleoprotein and by desoxyribonucleic acid (DNA). Corresponding tests, using charcoal, cellulose, Kaolin, diatomaceous earth and Di- galit as an adsorbent were negative, which indicates the specific nature of the adsorption by DNA. Proceeding from these findings, we tried to see whether the L.E.-factor could be detected by means of the passive hemagglutination test, when a nucleoprotein or DNA was used as the antigen. In order to investigate the specificity of this reaction, experiments with ribonucleic acid (RNA) from yeast, with desoxyribonucleoprotein and with DNA from calf thymus were carried out. We should like to report briefly the results obtained by this method.