Prevalence Of Human Antibodies Research Articles

Crimean-Congo Hemorrhagic Fever Virus in Humans and Livestock, Pakistan, 2015-2017.

We detected Crimean-Congo hemorrhagic fever virus infections in 4 provinces of Pakistan during 2017–2018. Overall, seroprevalence was 2.7% in humans and 36.2% in domestic livestock. Antibody prevalence in humans was highest in rural areas, where increased contact with animals is likely.

Seroprevalence and Risk Factors for Rickettsia and Leptospira Infection in Four Ecologically Distinct Regions of Peru.

Rickettsia and Leptospira spp. are under-recognized causes of acute febrile disease worldwide. Rickettsia species are often placed into the spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR). We explored the antibody prevalence among humans for these two groups of rickettsiae in four regions of Peru (Lima, Cusco, Puerto Maldonado, and Tumbes) and for Leptospira spp. in Puerto Maldonado and Tumbes. We also assessed risk factors for seropositivity and collected serum samples and ectoparasites from peri-domestic animals from households in sites with high human seroprevalence. In total, we tested 2,165 human sera for antibodies (IgG) against SFGR and TGR by ELISA and for antibodies against Leptospira by a microscopic agglutination test. Overall, human antibody prevalence across the four sites was 10.6% for SFGR (ranging from 6.2% to 14.0%, highest in Tumbes) and 3.3% for TGR (ranging from 2.6% to 6.4%, highest in Puerto Maldonado). Factors associated with seroreactivity against SFGR were male gender, older age, contact with backyard birds, and working in agriculture or with livestock. However, exposure to any kind of animal within the household decreased the odds ratio by half. Age was the only variable associated with higher TGR seroprevalence. The prevalence of Leptospira was 11.3% in Puerto Maldonado and 5.8% in Tumbes, with a borderline association with keeping animals in the household. We tested animal sera for Leptospira and conducted polymerase chain reaction (PCR) to detect Rickettsia species among ectoparasites collected from domestic animals in 63 households of seropositive participants and controls. We did not find any association between animal infection and human serostatus.

Detection of Crimean-Congo hemorrhagic fever virus-specific IgG antibodies in ruminants residing in Central and Western Macedonia, Greece

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus which causes lethal hemorrhagic fever in humans. Although, several reports regarding CCHFV antibody prevalence in humans exist in Greece, information about the current distribution is limited. The aim of the present study is to investigate the prevalence of CCHFV-specific IgG antibodies in cattle and sheep in Macedonia-Greece. The samplings were performed during spring 2013, in 5 regional units of Central Macedonia (Chalkidiki, Imathia, Kilkis, Pella and Thessaloniki) and in the 4 regional units of Western Macedonia (Grevena, Florina, Kastoria and Kozani). Specifically, sera from 538 cattle and 81 sheep underwent testing against CCHFV-specific IgG antibodies. Antiviral immune responses were observed in 31 cattle (6%, 95% CI: 4–8%) and in one sheep (1%, 95% CI: 0–8%). The total seroprevalence in the cattle sampled in Central Macedonia was 7% (28 out of 396, 95% CI: 5–10%). Within Central Macedonia, the highest seroprevalence was detected in Chalkidiki (38%, 95% CI: 23–56%), which was significantly higher (p<0.01) compared to the overall seroprevalence detected in cattle. In Western Macedonia, the total seroprevalence in cattle was 2% (3 out of 142, 95% CI: 1–7%). The 3 seropositive cattle were residing in the regional unit of Grevena. The one IgG-positive sheep serum was obtained from an animal residing in Thessaloniki. In this regional unit, the prevalence in sheep (2%, 95% CI: 0–10%) was much lower compared to the prevalence in cattle (12%, 95% CI: 6–22%), but significance was not achieved (p=0.03). The here presented seroepidemiological study demonstrates high transmission risk to human in specific geographical areas, which should be communicated to national and local public health authorities, so as to intensify preventive measures for public health protection.

Human Immune Proteome in Experimental Colonization with Staphylococcus aureus

More than 20% of adults are persistently colonized with Staphylococcus aureus. When hospitalized, these carriers have increased risks of infection with their own strains. However, a recent study demonstrated a lower incidence of bacteremia-related death among carriers than among noncarriers, raising the question whether the adaptive immune system plays a protective role. In fact, S. aureus carriers mount a highly specific neutralizing antibody response against superantigens of their colonizing strains. We now used 2-dimensional immunoblotting to investigate the profiles of antibodies from healthy individuals against S. aureus extracellular proteins. Moreover, we tested whether symptom-free experimental colonization of these individuals with an S. aureus strain of low virulence, 8325-4, is sufficient to induce an antibody response. Sera obtained before and 4 weeks after colonization were screened for immunoglobulin G (IgG) antibody binding to extracellular staphylococcal proteins. At baseline, most volunteers harbored IgG directed against conserved virulence factors, including alpha-hemolysin (Hla), beta-hemolysin (Hlb), phospholipase C (Plc), staphylococcal serine protease (SspA), and cysteine protease (SspB). However, the variability of spot patterns and intensities was striking and could be important in case of infection. Experimental nasal colonization with S. aureus 8325-4 did not elicit new antibodies or boost the humoral response. Thus, the high antibody prevalence in humans is likely not induced by short-term nasal colonization, and presumably minor infections are required to trigger anti-S. aureus antibody responses.

Foci of tick-borne diseases in Southwest Germany

Presently known tick-borne diseases in Germany include Lyme borreliosis, tick-borne encephalitis (TBE-virus, western subtype), Q-fever, babesiosis and presumably ehrlichiosis. Blood samples of 4,368 forestry workers in the State of Baden-Wuerttemberg (B-W), southwestern Germany, were tested for the presence of antibodies against Borrelia burgdorferi sensu lato, TBE-virus and Ehrlichia spp. (genogroup E. phagocytophila). Furthermore 12,327 ticks ( Ixodes ricinus) collected in various areas of B-W were analysed by PCR and genotyping for the prevalence of pathogen RNA and DNA. The human seroprevalence rates of antibodies to B. burgdorferi sensu lato ranged from 18 % to 52 %, for TBE-virus from 0 % to 43 % and for Ehrlichia spp. from 5 % to 16 % in various counties of the State. The foci of B. burgdorferi and TBE-virus as indicated by antibody prevalence in humans are only partly overlapping with each other. The highest rates of TBE-virus antibodies are in concordance with available clinical data. However antibody prevalence up to 27 % in areas with no reports of clinical cases was found, suggesting that TBE occurs throughout the State of B-W, The prevalence of Ehrlichia spp. antibodies suggests that ehrlichiosis plays a role as a tick-borne disease in Germany, but as long as no clinical data are available, this will remain unclear. Investigations of ticks for TBE-virus (n = 9,189) by nested PCR showed prevalence rates from 0% to 2.3% and for Ehrlichia spp. (n = 1,963) from 2.6% to 3.1%. Examination of ticks (n = 3,138) for the presence of B. burgdorferi sensu lato DNA was performed by PCR and revealed prevalence rates from 13.9% up to 24%. Furthermore 1,106 samples positive for B. burgdorferi sensu lato were used for genotyping. B. afzelii DNA was found in 407 ticks (36.8%), followed by B. garinii (21.9 %), B. valaisiana (13.7%), and B. burgdorferi sensu stricto (9.9 %). Double infection was found in 6.4% and triple infection in 0.8% of the ticks. 10.5% of the positive samples could not be classified. Prevention of tick-borne diseases has to focus on behavioural intervention to reduce individual tick exposure by proper behaviour in the environment, as a large-scale control of the tick population seems impossible and thus reduction of Lyme borreliosis and TBE through tick control is unlikely. Vaccination against TBE-virus should not only be recommended for high endemic areas but also for persons with a high individual risk.

Risk of human infections with Crimean-Congo hemorrhagic fever virus in a South African rural community.

Crimean-Congo hemorrhagic fever (CCHF) virus is widely distributed in wild and domestic mammals, birds, and ticks throughout many regions of Africa, Europe, and Asia. Interviews were conducted with 484 individuals from nine farms in the Republic of South Africa from which recent human CCHF cases had originated and with individuals from 27 farms without recognized cases. Serum samples were obtained from all consenting individuals. Blood was also drawn from 2,212 farm animals. Human infection with CCHF virus was uncommon (point prevalence 12.6/1,000). Antibody prevalence in humans on farms increased with age (P less than 0.001), and was correlated with handling lambs. Overall, a greater number of older animals were antibody positive than animals less than one year of age (P less than 0.001), but 12.7% of young animals on farms with human were antibody positive compared with 5.8% on those farms without human infection (P less than 0.05). Physical contact with ticks or tick bite was also found to be a risk factor, but contact with animal blood or fresh meat was not. The risk of CCHF virus infection in the community increased seven-fold following contact with a recognized CCHF case, even when other risk factors were taken into account (point prevalence rate 4.7%). In contrast, antibody prevalence was less than 1% (1 of 128) in the local hospital staff who had cared for patients with CCHF. Prevention is best achieved by education of the farming community and establishing and maintaining awareness in the hospital staff.

Case-control study of Mastomys natalensis and humans in Lassa virus-infected households in Sierra Leone.

Lassa virus infection and antibodies were studied in households where Lassa fever cases occurred, and compared to those in nearest neighbor houses and "far" houses located across the village from case houses. Seventy-nine percent of all rodents caught in the houses were Mastomys, the natural reservoir of Lassa virus. Rodent infection was not randomly distributed, but rather focal. Thirty-nine percent of the Mastomys in case houses were viremic, compared to 3.7% in control houses. Human antibody prevalence in case houses was 30%, compared to 20% in non-case houses (P less than 0.05, chi-square test, df = 2). Neither seroconversions nor antibody prevalence rates were associated with household size or number of persons per room. Trapping of rodents in half of the case and control houses resulted in a Mastomys reduction ranging from 2.2- to 3.3-fold. This reduction failed to significantly reduce the seroconversion rate to Lassa virus in the people of trapped houses compared to those in untrapped ones. More complete trapping will be needed in order to better evaluate this procedure as a means of interruption of Lassa virus transmission in endemic villages.

Endemicity of toxoplasmosis in Costa Rica.

Toxoplasma infection can be transmitted to humans by ingestion of tissue cysts in meat or by ingestion of oocysts in cat feces or fecally contaminated soil. since meat is traditionally well cooked in Costa Rica and the authors were able to exclude meat and eggs from playing a role in transmission, they postulated that ingestions of oocysts should entirely explain transmission. To test this hypothesis, they studied seven populations in Costa Rica and serologically tested 883 people between 15 and 26 years of age. By interviews and surveys, they determined mode of living, soil contact, cat contact, and cat density. The infection rate in cats was studied by stool and serologic examination, and soil availability for defecation was determined from aerial photographs. Antibody was taken as indicating infection and was found to be acquired most actively in children and young cats. In a retrospective analysis, antibody prevalence in humans correlated highly with individual cat contact (both owned and stray), with cat density, with living in houses with wood floors and a crawl space underneath, and with living with cats on a cement floor. antibody prevalence in humans correlated negatively with soil availability to cats for defecation and with soil contact by humans, suggesting that oocysts may be transmitted optimally near house in areas where most terrain is covered by asphalt and concrete and little soil is available for defecation or for human soil contact. Cats acquire infection by eating intermediate hosts; 16% sparrows, 3.5% of mice and 12.5% of rats have been found infected. Favoring transmission are young non-immune cat populations, shade and moisture, older intermediate hosts, a high percentage of which have become infected, and concentration of houses in city blocks. Transmission is diminished when houses are dispersed or arranged along a road in an open rural setting, permitting dispersal of cat fecal deposits.

Ecologic studies of vesicular stomatitis virus. I. Prevalence of infection among animals and humans living in an area of endemic VSV activity.

Tesh, R. B., P. H. Peralta and K. M. Johnson (Middle America Research Unit, Box 2011, Balboa Heights, Canal Zone). Ecologic studies of vesicular stomatitis virus. I. Prevalence of infection among animals and humans living in an area of endemic VSV activity. Amer. J. Epid., 1969, 90: 255–261.—The prevalence of VSV neutralizing antibodies in various Panamanian human and animal populations was studied. Among wild animals, VSV-lndiana antibodies were found mainly in arboreal and semiarboreal species. VSV-New Jersey infection rates in feral animals were highest in Chiroptera, Carnivora, and certain rodents. Domestic animals and humans had high antibody rates to both VSV-lndiana and New Jersey. The antibody prevalence in humans increased with age, suggesting a direct relation between VSV infection rates and length of residence in the endemic area. Geographic and species differences in antibody rates between VSV-lndiana and New Jersey imply that the two virus serotypes may have different cycles in nature. Available evidence suggests that VSV-lndiana is arthropod-transmitted; the mode of VSV-New Jersey transmission is unknown.