Antibiotic Resistance Genes In Sludge Research Articles

Temperature-phased anaerobic sludge digestion effectively removes antibiotic resistance genes in a full-scale wastewater treatment plant

Sludge is a major by-product and the final reservoir of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs). Temperature-phased anaerobic digestion (TPAD), consisting of thermophilic anaerobic digestion (AD) (55 °C) and mesophilic AD processes (37 °C), has been implemented in WWTPs for sludge reduction while improving the biomethane production. However, the impact of TPAD on the ARGs' fate is still undiscovered in lab-scale experiments and full-scale WWTPs. This study, for the first time, investigated the fate of ARGs during the TPAD process across three seasons in a full-size WWTP. Ten typical ARGs and one integrase gene of class 1 integron (intI1) involving ARGs horizontal gene transfer were examined in sludge before and after each step of the TPAD process. TPAD reduced aac(6′)-Ib-cr, blaTEM, drfA1, sul1, sul2, ermb, mefA, tetA, tetB and tetX by 87.3–100.0 %. TPAD reduced the overall average absolute abundance of targeted ARGs and intI1 by 92.39 % and 92.50 %, respectively. The abundance of targeted ARGs in sludge was higher in winter than in summer and autumn before and after TPAD. During the TPAD processes, thermophilic AD played a major role in the removal of ARGs, contributing to >60 % removal of ARGs, while the subsequent mesophilic AD contributed to a further 31 % removal of ARGs. The microbial community analysis revealed that thermophilic AD reduced the absolute abundance of ARGs hosts, antibiotic resistant bacteria. In addition, thermophilic AD reduced the abundance of the intI1, while the intI1 did not reproduce during the mesophilic AD, also contributing to a decline in the absolute abundance of ARGs in TPAD. This study demonstrates that TPAD can effectively reduce the abundance of ARGs in sludge, which will suppress the transmission of ARGs from sludge into the natural environment and deliver environmental and health benefits to our society.

Effects of antibiotic exposure on bioreactor treatment efficiency and dynamic migration of resistance genes in landfill leachate

Different concentrations of tetracycline (TC) (10 and 30 mg/L) were added to the anaerobic-anoxic-aerobic reactor to investigate the changes in effluent quality, sludge performance and the dynamic migration of antibiotic resistance genes (ARGs) in simulated landfill leachate. The maximum removal rates of COD, TN, and TP occurred at a TC concentration of 10 mg/L, reaching 75.99 %, 50.46 %, and 60.26 %, while the removal efficiency of NH3-N continued to decline. Microorganisms secreted abundant extracellular polymeric substance (EPS) and combined with antibiotic to form a stable and dense EPS antibiotic complex to resist adverse stress. The concentration of heavy metals showed a trend of first increasing and then decreasing, especially Mn and Zn. At a concentration of 10 mg/L TC, aerobic condition weakened ARGs, while anaerobic facilitated the proliferation of ARGs. The tetA and tetM in leachate were positively correlated with TC, TP, TN, NH3-N, and COD (P < 0.05), while the diffusion and transmission of ARGs in sludge were under the superimposed pressure of TC, various heavy metals, and EPS. Moreover, changes in bacterial communities drove the release of ARGs. To analyze the potential risks of ARGs in landfill leachate under antibiotic exposure, providing clues for the management and risk assessment of sewage biological treatment facilities.

Distribution and dynamics of antibiotic resistance genes in sludge under discharge plasma oxidation

Antibiotic resistance genes (ARGs), emerging environmental contaminants, have been largely accumulated in sludge during wastewater treatment. Discharge plasma oxidation, an advanced oxidation technology, has been demonstrated as an effective strategy in sludge pretreatment. However, the effect of plasma oxidation on the distribution and dynamics of ARGs in sludge is unknown. Herein, the variations of four typical ARGs (tetC, tetW, blaTEM-1, aac(3)-Ⅱ) and one integron (intI-1) in sludge during discharge plasma treatment were investigated. The results showed that under low discharge voltage or short treatment time, weak plasma oxidation enriched the contents of ARGs and intI-1 in sludge (with 1.14 log unit maximum increment) via proliferating biomass or promoting horizontal gene transfer. Further increasing discharge voltage or prolonging treatment time, strong plasma oxidation eliminated target genes due to the destroyed bacteria or inhibited horizontal transfer of ARGs. Especially, the homogeneous condition greatly promoted the reduction of ARGs in liquid phase (up to 1.52 log unit reduction). Furthermore, the correlation analysis between intI-1 and ARGs implied that tetC and tetW abundances might be significantly affected by intI-1 through horizontal transfer. At the same time, the microbial communities in sludge were investigated to help understand the effect of plasma oxidation on ARGs variation. Notably, the most predominant phylum Proteobacteria and genus Acinetobacter resisted the oxidative infringement from plasma to maintain high relative abundances (52.05 % in solid phase and 84.10 % in liquid phase for Proteobacteria, 13.82 % in solid phase and 78.00 % in liquid phase for Acinetobacter at 20 kV). Meanwhile, the co-occurrence analysis between genes and dominant genera further showed that Romboutsia sp. might be a potential host bacteria of ARGs and intI-1 in solid phase, and Acidovorax sp., Parachlamydia sp., and Armatimonadetes_gp5 sp. might be the potential hosts of tetW, tetC, and intI-1 in liquid phase.

Insight into the evolution of microbial communities and resistance genes induced by sucralose in partial nitrification system with triclosan pre-exposure

Sucralose (SUC), an artificial sweetener widely used in food, beverages and pharmaceuticals, is frequently detected in various environmental matrices. Triclosan (TCS) is commonly used as a disinfectant and often co-exists with SUC in sewage environments. This study investigated the effects of SUC (0.1–10 mg/L) on the transmission of intracellular and extracellular antibiotic resistance genes (ARGs) in the partial nitrification systems with and without TCS pre-exposure. The reactors operated for 150 days, and SUC did not affect ammonia oxidation performance, while TCS led to the maintenance of partial nitrification. The types and abundances of extracellular ARGs in sludge and free ARGs in water increased significantly after TCS pre-exposure when faced SUC stress, which might be caused by a decrease in α-Helix/(β-Sheet + Random coil). SUC was more easily to enrich ARGs in partial nitrification systems with TCS pre-exposure, exacerbating the risk of ARGs transmission. The microbial community showed stronger relationships to cope with the direct stress of SUC, and the functional bacteria (Thauera and Nitrosomonas) in TCS pre-exposure system might be potential hosts of ARGs. This study might provide insights for better understanding the fates of SUC in partial nitrification systems and the ecological risks in wastewater containing TCS and SUC. Environmental ImplicationSucralose (SUC) is often detected in the environment and considered as an emerging contaminant due to its soaring consumption and environmental persistence. Triclosan (TCS) is an antibacterial agent that often co-exists with SUC in personal care products and sewage environments. During 150 d, two partial nitrification reactors with and without TCS pre-exposure were established to study the effects of SUC on nitrification performance, antibiotic resistance genes (ARGs) and microbial communities. This study showed the refractory nature of SUC, and SUC led to the transmission of extracellular ARGs in partial nitrification system with TCS pre-exposure, exacerbating the risk of ARGs dissemination.

Microplastics into vermi-wetland lower the treatment performance of organic substances and antibiotic resistance genes in excess sludge

This study aimed to investigate the effects of MPs on the decomposition of excess sludge and reduction of antibiotic resistance genes (ARGs) in a vermi-wetland. For this, the polymethyl methacrylate (PMMA) MPs added into excess sludge were treated by a vermi-wetland inoculated by earthworms Eisenia fetida and wetland plant Acorus calamus. The results showed that the growth rates of plants and earthworms were significantly inhibited in the vermi-wetland added with MPs. The average removal rates of chemical oxygen demand and total solids for excess sludge in vermi-wetland added MPs decreased by 15.36% and 38.00%, respectively, compared to the control without adding MPs. Moreover, the abundance of 16 S rDNA and 18 S rDNA inside vermi-wetland with MPs enriched by 53.61% and 75%, respectively. In addition, the median removal rate of selected ARGs in sludge declined by 15.75%−34.27% in the MPs added vermi-wetland. Compared to the control, the MPs enhanced the mobile genetic elements of intI 1 and tnpa-04 abundance inside the vermi-wetland by 44.79% and 53.33%, respectively, which were separately attached on the MPs surface at 25.62 ng/g and 7.26 ng/g. This study suggests that the addition of MPs into vermi-wetland reduced the removal efficiency of organic substances and ARGs in the excess sludge.

Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes.

Surfactants could influence sludge morphology and disinfectants were linked to antibiotic resistance genes (ARGs). Thus, the response of activated sludge and ARGs to long-term quaternary ammonium compounds (QACs) exposure required further investigation, which is a popular surfactant and disinfectant. Here, three sequencing batch reactors were fed with 5mg/L most frequently detected QACs (dodecyl trimethyl ammonium chloride (ATMAC C12), dodecyl benzyl dimethyl ammonium chloride (BAC C12) and didodecyl dimethyl ammonium chloride (DADMAC C12)) for 180 d. The long-term inhibitory effect on denitrification ranked: DADMAC C12>BAC C12>ATMAC C12. Besides, obvious granular sludge promoted by the increase of α-Helix/(β-Sheet + Random coil) appeared in DADMAC C12 system. Moreover, intracellular ARGs increased when denitrification systems encountered QACs acutely but decreased in systems chronically exposed to QACs. Although replication and repair metabolism in ATMAC C12 system was higher, ATMAC C12 significantly promoted proliferation of extracellular ARGs. It was noteworthy that the propagation risk of extracellular ARGs in sludge increased significantly during sludge granulation process, and intracellular sul2 genes in sludge and water both increased with the granular diameter in DADMAC C12 system. The universal utilization of QACs may enhance antibiotic resistance of bacteria in wastewater treatment plants, deserving more attention.

Fate of intracellular, extracellular polymeric substances-associated, and cell-free antibiotic resistance genes in anaerobic digestion of thermally hydrolyzed sludge

Thermal hydrolysis of sludge is a promising approach to mitigate antibiotic resistance genes (ARGs) propagation in anaerobic digestion (AD). Although ARGs in sludge may be fractioned into intracellular, extracellular polymeric substance (EPS)-associated, and cell-free ARGs, the fate of these different fractions in AD has never been investigated. This study presents a detailed characterization of intracellular and extracellular ARGs in AD of sludge thermally hydrolyzed at 90 °C and 140 °C. EPS-associated ARGs represented the major fraction of the total extracellular ARGs in all samples, while its lowest abundance was observed for thermal hydrolysis at 140 °C along with the lowest EPS levels. The results suggested a positive correlation between EPS-associated ARGs with intracellular and cell-free ARGs. Furthermore, various EPS components, such as proteins and e-DNA, were positively correlated with β-lactam resistance genes. sul1 dominated all samples as an EPS-associated resistance gene. These results provide new insights into the significance of different ARGs fractions in their overall dissemination in AD integrated with thermal hydrolysis.

Fate of antibiotic resistance genes (ARGs) in wastewater treatment plant: Preliminary study on identification before and after ultrasonication

This study collected sludge samples from four different sections of a local wastewater treatment plant in Mikkeli, Finland, for antibiotic resistance genes (ARGs) analysis. Here, we examine the seven representative ARGs in sludge, encoding erythromycin (ermB), tetracycline (tetA, tetC, tetQ, tetW) and sulphonamide (sul1) to check abundance before and after ultrasonication. The class 1 integron (intl1) was also observed as an indicator of antibiotic resistance and horizontal gene transmission. The pre-treatment condition included 10 min of ultrasonication (US) for the sludge sample before freeze-drying. The droplet digital PCR system was used to assess the ARGs from the samples, and it was found that ARGs were not effectively eliminated by pre-treatment. After ultrasonication, tetA, tetC and tetQ did not show any variation but tetW showed 20 copies/ng of lower abundance in digested sludge than raw sludge, and a similar abundance was found in dewatered sludge. For MBR sludge, only ermB showed 1000 copies/ng higher abundance compared to the raw sample and surprisingly it did not show the presence of any other types of ARG. This study provides an overview of the appearance of ARGs in regional municipal sludge for further research reflection.

Anaerobic electrochemical membrane bioreactor effectively mitigates antibiotic resistance genes proliferation under high antibiotic selection pressure.

The spread of antibiotics and antibiotic resistance genes (ARGs) in environments has posed potential threats to public health. Unfortunately, conventional biological wastewater treatment technologies generally show insufficient removal of antibiotics and ARGs. Bioelectrochemical systems, which can effectively degrade refractory organic pollutants via enhancing microbial metabolisms through electrochemical redox reaction, may provide an alternative for the control of antibiotics and ARGs. Herein, an anaerobic electrochemical membrane bioreactor (AnEMBR) was conducted by combining bioelectrochemical system and anaerobic membrane bioreactor to treat antibiotic-containing wastewater. The AnEMBR at open circuit showed stable CH4 production and high removal of COD and chlortetracycline (CTC) in treating 2.5-15mg/L CTC. However, increasing CTC to 45mg/L completely inhibited the methanogenesis of AnEMBR at open circuit. After applying external voltage in AnEMBR, the performances of AnEMBR were significantly improved (e.g., increased CH4 production and CTC removal). Moreover, CTC exposure significantly increased the relative abundances of ARGs in sludge, supernatant, and effluent in AnEMBR at open circuit. Applying voltage greatly attenuated the total relative abundances of ARGs in the supernatant and effluent of AnEMBR compared to those at open circuit. This could be attributed to the enrichment of tetracycline degradation gene tetX, which greatly enhanced the removal of CTC by the AnEMBR and thus reduced the selective pressure of CTC on the microorganisms in supernatant and effluent for ARGs proliferation. These results would provide an effective wastewater treatment technology for treating high-level antibiotic-containing wastewater to mitigate the potential risk of ARGs and antibiotics spread in receiving water body.

The removal of antibiotic resistance genes by different biological treatment processes of four wastewater treatment plants in various seasons

The widespread antibiotic resistance genes (ARGs) severely jeopardizing human health have been widely concerned. Wastewater treatment plants (WWTPs) are commonly considered the important sources and sinks of ARGs due to their roles in depositing ARGs and provoking ARGs' proliferation. Current WWTPs often use biological treatment to remove pollutants, whereas the purification efficacy of ARGs is unclear. Thus, this study investigated the seasonal changes of ARGs (tetA, tetC, tetM, tetO, tetQ, tetW, and tetX, as representative) and a class of integron (intI1) in the sludge of different treatment units of four WWTPs to understand the role of WWPTs in ARGs spread. It is found that ARGs abundance varied between summer and winter depending on the types of ARGs and treatment units. The cumulative abundance of ARGs in the same treatment unit showed no obvious difference between seasons. By comparison, ARGs abundance was generally lower in the anoxic unit than in the oxic unit. Moreover, the WWTPs with Cyclic Activated Sludge System (CASS) process reduced the amount of ARGs in sludge, while the WWTPs with Anaerobic-Anoxic-Oxic (A2O) increased ARGs abundance. In conclusion, this study demonstrated that the excess sludge still posed a risk for ARG spread after biological treatment.

Effects of Anaerobic Digestion on Antibiotic Resistance Genes in Sludge

Sludge treatment and reuse in municipal sewage treatment plants is beneficial to improve economic effect and reduce environmental pollution. Sludge is an important source of contamination of antibiotic resistance genes. Anaerobic fermentation is an important way to treat activated sludge. Effective treatment of municipal sludge can avoid the release of pathogenic microorganisms and resistance genes (ARGs) into the environment and cause secondary pollution. The research results will be of certain guiding significance to the resource utilization of sludge.

Distribution of extracellular and intracellular antibiotic resistance genes in sludge fractionated in terms of settleability

The widespread proliferation of antibiotic resistance genes (ARGs) is a serious environmental and human health issue. Wastewater treatment facilities (WWTFs) are potential sources to spread ARGs to natural environment, for which, the presence state of ARGs in the sludge, as extracellular ones (eARGs) or intracellular ones (iARGs), along with the sludge settleability, are very important factors. The sludge settleability is closely associated with its floc size and density, bacterial activity, and the proportion of intact/damaged bacterial cells that aggregate together to form flocs for separation in the sedimentation process. It is reasonable to hypothesize that the distribution of eARGs and iARGs may differ with the sludge fractions of different settleability, a topic of great academic and practical significance requiring clarification. In this study, sludge samples from the aerobic contact tank of six household WWTFs were fractionated into fractions with different settling velocities: sludge of low settleability (LS), medium settleability (MS) and high settleability (HS); and the distribution of eARGs and iARGs in the obtained fractions for the widely detected tet G, tet M and sul 1 in water environment was evaluated based on the PMA-qPCR method, together with the evaluation for the well reported mobile genomic element intl 1 and total bacterial 16S rDNA. For the LS fractions, which contained more damaged bacterial cells, the distribution percentages of eARGs were generally higher than those of iARGs. For the HS fractions, which contained flocs with larger sizes formed by both intact and damaged bacterial cells, the relative abundances of ARGs and intl 1 were found apparently lower even if the presence percentages of eARGs were comparatively higher. It is thus inferable that sludge fractions of LS may possess higher transfer potential for ARGs and enhancing their settleability through optimization of the operation conditions is important for mitigating the proliferation of ARGs.

Insight into effect of high-level cephalexin on fate and driver mechanism of antibiotics resistance genes in antibiotic wastewater treatment system.

In the study, antibiotic resistance genes (ARGs) were examined in wastewater and sludge samples to explore the effect of cephalexin (CFX) on the spreading and removal of ARGs in the Expanded Granular Sludge Bed (EGSB) reactor treating antibiotics wastewater. The result showed that the addition of CFX in the wastewater affected the removal amount of β-lactam ARGs and other types ARGs. Besides, the addition of CFX in the wastewater had no obviously effect on total concentration of targeted ARGs in the sludge, but it was related to the accumulation of some typical ARGs. Based on gene cassette array libraries analysis, the diversity of gene cassettes carried by intI1 gene was increased by the addition of CFX in the wastewater. Furthermore, the co-occurrence patterns between ARGs and bacterial genus were also investigated. The results showed the CFX in the wastewater not only affected the number of potential host bacteria of ARGs, but also changed the types of potential host bacteria of ARGs. The correlation analysis of ARG in influent, effluent and sludge showed that, for blaCTX-M, sul2, qnrS and AmpC genes, their removal amount in EGSB reactor treating antibiotic wastewater system might be enhanced by reducing their concentration in the sludge.

Conditioning with zero-valent iron or Fe2+ activated peroxydisulfate at an acidic initial sludge pH removed intracellular antibiotic resistance genes but increased extracellular antibiotic resistance genes in sewage sludge

Sulfate radical (SO4−)-based conditioning methods, such as zero-valent iron (ZVI, i.e., Fe0) or ferrous iron (Fe2+) activated peroxydisulfate (S2O82-), have recently developed to improve sludge dewaterability, but it remains unclear how they impact the intracellular and extracellular antibiotic resistance genes (ARGs) in sewage sludge. In this study, it was found that conditioning treatments that used ZVI/S2O82- or Fe2+/S2O82- system, at an acidic initial sludge pH, removed the intracellular ARGs and intI1 and the extracellular intI1 from sewage sludge, but led to the accumulation of extracellular ARGs of aadA-01, aadA-02, aadA1, aadA2-03, and strB in conditioned sludge. During sludge conditioning with ZVI/S2O82- or Fe2+/S2O82-, bacterial hosts of ARGs and intI1 were seriously lysed to release the intracellular ARGs and intI1 to the extracellular environment, thus removing intracellular ARGs and intI1 in sludge, while the released ARGs and intI1 were primarily degraded by the produced SO4− to attenuate most extracellular ARGs and intI1. However, the relatively lower degradation ability of SO4− for extracellular ARGs of aadA-01, aadA-02, aadA1, aadA2-03, and strB led to their accumulation in conditioned sludge. Therefore, SO4−-based conditioning methods can be employed to reduce ARGs in sludge, but the subsequent treatment of sludge dewatering filtrate requires more attention.

Importance of sludge conditioning in attenuating antibiotic resistance: Removal of antibiotic resistance genes by bioleaching and chemical conditioning with Fe[III]/CaO

Conditioning can drastically improve the dewaterability of sewage sludge and thus it is widely practiced in most wastewater treatment plants (WWTPs). In WWTPs, various antibiotic resistance genes (ARGs) present in sewage are concentrated in the sewage sludge, but the effect of sludge conditioning on ARGs in sewage sludge remains unclear. Here, we evaluated and compared the effectiveness of four sludge conditioning methods (namely chemical conditioning with polyacrylamide (PAM), chemical conditioning with Fe[III]/CaO, bioleaching conditioning, and chemical acidification conditioning) and an aerobic incubation control in removing 46 target ARGs and intI1 from a municipal sewage sludge. The damage of sludge microbial cells and the change in the sludge bacterial community during the various sludge conditioning treatments were also characterized. The results suggested that the chemical conditioning with PAM and aerobic incubation treatment did not remove ARGs and intI1 from the sewage sludge. The chemical acidification reduced the absolute abundances of most ARGs and intI1, but increased their relative abundances. However, the chemical conditioning with Fe[III]/CaO and bioleaching conditioning reduced both the absolute and relative abundances of most ARGs and removed a majority of extracellular ARGs in the sludge. During sludge conditioning treatments, the sludge microbial cells were severely damaged to decrease the total bacterial biomass in sludge, and accordingly the bacterial hosts carrying ARGs and intI were effectively damaged to reduce the absolute abundances of most ARGs and intI1. In addition, the sludge bacterial community in conditioned sludge determined the relative abundances of residual ARGs. Our findings suggest that sludge conditioning can be an important sludge treatment process in attenuating antibiotic resistance in sewage sludge, and bioleaching and chemical conditioning with Fe[III]/CaO can be employed as effective conditioning ways to reduce ARGs in sewage sludge, potentially limiting their release to the environment.

Deciphering the factors influencing the discrepant fate of antibiotic resistance genes in sludge and water phases during municipal wastewater treatment

The discrepant fate of antibiotic resistance genes (ARGs) in sludge and water phases was investigated in a municipal wastewater treatment plant, and a lab-scale A2O-MBR was operated to provide background value of ARGs. The influencing factors of ARGs including microbial community, co-selection from heavy metals, biomass and horizontal gene transfer were concerned. Results showed that iA2O (inversed A2O) showed better ARGs reduction, and longer SRT (sludge retention time) increased ARGs relative abundance while reduced the gene copies of ARGs in the effluent, but significantly increased the ARGs in sludge phase. Compared to background value, the most enriched ARG was tetX in water phase, while it was intI1 in sludge phase. There existed higher abundance of multi-resistant bacteria in sludge phase, and microbial community determined the fate of ARGs in both water and sludge phase, while the direct effects from horizontal gene transfer should not be overlooked especially in water phase.

Alkaline fermentation of waste sludge causes a significant reduction of antibiotic resistance genes in anaerobic reactors.

Alkaline fermentation has been reported to be an effective method to recover valuable products from waste sludge. However, to date, the potential effect of alkaline pH on the fate of antibiotic resistance genes (ARGs) during anaerobic fermentation of sludge has never been documented. In this study, the target ARGs in sludge was observed to be removed effectively and stably when sludge was anaerobically fermented at pH10. Compared with the control (without pH adjustment), the abundances of target ARGs at pH10 were reduced by 0.87 (sulI), 1.36 (sulII), 0.42 (tet(O)), 1.11 (tet(Q)), 0.79 (tet(C)) and 1.04 (tet(X)) log units. Further investigations revealed that alkaline fermentation shifted the community structures of potential ARGs hosts. Moreover, alkaline fermentation remarkably decreased the quantities and the ARGs-possessing ability of genetic vectors (plasmid DNA, extracellular DNA and phage DNA), which might limit the transfer of ARGs via conjugation, transformation and transduction. These results suggest that the shifted compositions of gene hosts and restricted gene transfer potential might be the critical reasons for the attenuation of ARGs at pH10.

High Levels of Antibiotic Resistance Genes and Their Correlations with Bacterial Community and Mobile Genetic Elements in Pharmaceutical Wastewater Treatment Bioreactors.

To understand the diversity and abundance of antibiotic resistance genes (ARGs) in pharmaceutical wastewater treatment bioreactors, the ARGs in sludge from two full-scale pharmaceutical wastewater treatment plants (PWWTPs) were investigated and compared with sludge samples from three sewage treatment plants (STPs) using metagenomic approach. The results showed that the ARG abundances in PWWTP sludge ranged from 54.7 to 585.0 ppm, which were higher than those in STP sludge (27.2 to 86.4 ppm). Moreover, the diversity of ARGs in PWWTP aerobic sludge (153 subtypes) was higher than that in STP aerobic sludge (118 subtypes). In addition, it was found that the profiles of ARGs in PWWTP aerobic sludge were similar to those in STP aerobic sludge but different from those in PWWTP anaerobic sludge, suggesting that dissolve oxygen (DO) could be one of the important factors affecting the profiles of ARGs. In PWWTP aerobic sludge, aminoglycoside, sulfonamide and multidrug resistance genes were frequently detected. While, tetracycline, macrolide-lincosamide-streptogramin and polypeptide resistance genes were abundantly present in PWWTP anaerobic sludge. Furthermore, we investigated the microbial community and the correlation between microbial community and ARGs in PWWTP sludge. And, significant correlations between ARG types and seven bacterial genera were found. In addition, the mobile genetic elements (MGEs) were also examined and correlations between the ARGs and MGEs in PWWTP sludge were observed. Collectively, our results suggested that the microbial community and MGEs, which could be affected by DO, might be the main factors shaping the profiles of ARGs in PWWTP sludge.