Antibacterial Properties Of Chitosan Research Articles

Chitosan-Based Scaffolds Incorporated with Silver Nanoparticles for the Treatment of Infected Wounds.

Bacterial infections are major problems in wound care due to their impact on the retarded process of wound healing, leading to chronic wounds. Most of the presently utilized wound dressing products exhibit poor antimicrobial properties. Wound dressings formulated from chitosan have been reported to be effective for treating infected wounds, resulting from the antibacterial properties of chitosan. The antibacterial properties of chitosan-based wound dressings can be further enhanced by incorporating metallic nanoparticles into them, such as silver, zinc, titanium, etc. The incorporation of silver nanoparticles into chitosan-based wound dressings has been widely explored in the design of antimicrobial wound dressings. The incorporation of silver nanoparticles into chitosan-based wound dressings promotes accelerated wound-healing processes due to enhanced antimicrobial activity. The accelerated wound healing by these metal-based nanoparticles is via the regulation of re-epithelialization and inflammation without affecting the viability of normal cells. However, there have been few reports that evaluate these wound dressings in infectious animal models to prove their efficacy. The in vivo toxicity of silver nanoparticles still needs to be addressed, revealing the need for further preclinical and clinical trials. The fabrication of wound dressings incorporated with silver nanoparticles has not been fully explored, especially for wounds requiring immediate treatment. The possible interactions between silver nanoparticles and chitosan scaffolds that result in synergistic effects still need to be understood and studied. This review provides a comprehensive report on the preclinical outcomes of chitosan wound dressing materials loaded with silver nanoparticles for managing infected wounds.

Development of anti-bacterial adhesion and antibacterial sulfobetaines modified chitosan/polyvinyl alcohol composite films as packaging materials

Chitosan exhibits a wide source, non-toxic and biodegradable, and is the optimal functional raw material for preparing food packaging materials. However, the pure chitosan film has some disadvantages such as limited antibacterial activity and weak mechanical properties. In this study, sulfobetaines modified chitosan (CS-SBMA) was synthesized by grafting copolymerized betaine methacrylate sulfonate onto the chain of chitosan to improve the anti-bacterial adhesion and antibacterial properties of chitosan, aiming to develop antibacterial and anti-bacterial adhesion films based on CS-SBMA and polyvinyl alcohol (PVA) by the casting method. The structure of CS-SBMA was characterized by 1H NMR and FTIR. The appropriate proportion of CS-SBMA/PVA was determined to be 1/1 and 1/2, by characterizing the composite films with FTIR, XRD, SEM, mechanical, optical, and water resistance behaviors. In addition, CS-SBMA/PVA films showed excellent antibacterial, anti-bacterial adhesion and biofilm control function. The colonies number of E. coli and S. aureus on the surface of CS-SBMA/PVA 1/1 film decreased 94.15 % and 94.27 %, respectively, and 92.93 % of S. aureus and 94.87 % of E. coli colonies were inactivated within 60 min contact. These results indicate that CS-SBMA/PVA film exhibits potential antibacterial and anti-bacterial adhesion properties, which is suitable for food packaging materials.

An antibacterial nanoclay- and chitosan-based quad composite with controlled drug release for infected skin wound healing

Microbial infections of surgical sites and other wounds represent a major impediment for patients. Multifunctional low-cost dressings promoting tissue reparation while preventing infections are of great interest to medical professionals. Here, clay-based laponite nanodiscs (LAP) were loaded with the antibacterial drug kanamycin (KANA) before being embedded into a poly(lactic-co-glycolic acid) (PLGA) membrane and coated with the biopolymer chitosan (CS). Results indicated that these biocompatible materials combined the excellent capacity of LAP for controlled drug release with the mechanical robustness of PLGA and the antibacterial properties of CS as well as its hydrophilicity to form a composite highly suitable as an infection-preventing wound dressing. In vitro, PLGA/LAP/KANA/CS released drugs in a sustainable manner over 30 d, completely inhibited the growth of infectious bacteria, prompted the adhesion fibroblasts, and accelerated their proliferation 1.3 times. In vivo, the composite enabled the fast healing of infected full-thickness skin wounds with a 96.19 % contraction after 14 d. During the healing process, PLGA/LAP/KANA/CS stimulated re-epithelization, reduced inflammation, and promoted both angiogenesis and the formation of dense collagen fibers with an excellent final collagen volume ratio of 89.27 %. Thus, multifunctional PLGA/LAP/KANA/CS made of low-cost components demonstrated its potential for the treatment of infected skin wounds.

Electrochemical, third order nonlinear optical and antibacterial properties of chitosan, a cationic polymer loaded CuS:Co nanoparticles

Pure and 5, 10 and 15 wt % Co-doped CuS (CuS:Co) nanoparticles were prepared by precipitation method. Diffraction peaks related to hexagonal structure was observed. Decreased crystallite size was realized as Co content increases. The inclusion of Co in the doped samples was confirmed by EDS spectra. Increased rate of radiative recombination increased the PL intensities of the doped samples. Co doping improved electrochemical, third order NLO, magnetic, and antimicrobial characteristics. The 10 wt percent co-doped CuS sample, which has superior electrochemical, magnetic, NLO, and antibacterial capabilities, was produced utilising cationic polysaccharide chitosan in a green manner. Chitosan untreated and loaded 10 wt% Co-doped CuS NPs have crystallite sizes of 61 and 55 nm, respectively. The interaction of CuS:Co NPs with chitosan biopolymer leads in improved electrochemical behaviour for the chitosan-loaded sample. The effective attraction between chitosan amino groups and the bacterial cell membrane results in enhanced antibacterial property for the chitosan loaded sample.

Asymmetric wettable polycaprolactone-chitosan/chitosan oligosaccharide nanofibrous membrane as antibacterial dressings

Wound infection and inflammation hinder the process of wound healing and bother human beings chronically. As a naturally degradable macromolecule, chitosan (CS) has been widely used in antibacterial wound dressings. However, the antibacterial property of chitosan is inhibited by its water insolubility. In this study, we prepared a bilayered asymmetric nanofibrous membrane with the hydrophilic CS/chitosan oligosaccharide (COS) nanofibrous membrane as the bottom layer and the hydrophobic polycaprolactone (PCL) nanofibrous membrane as the top layer. Results showed that incorporating COS improved the CS membrane's wettability, and adding 0.5 % COS increased the inhibition zone diameter of Escherichia coli and Staphylococcus aureus by 23 % and 26 %, respectively. Moreover, the PCL layer could prevent the adhesion of water and bacteria. The PCL-CS/COS0.5% membrane showed relatively good mechanical properties, excellent water absorptivity (460 %), and appropriate cytocompatibility. This asymmetric wettable membrane has a massive potential to serve as a new antibacterial dressing for wound healing.

Preparation and characterization of vaccarin, hypaphorine and chitosan nanoparticles and their promoting effects on chronic wounds healing

Chronic wounds have become an important factor hindering human health, affecting tens of millions of people worldwide, especially diabetic wounds. Based on the antibacterial properties of chitosan, the angiogenesis promoting effect of vaccarin (VAC) and the anti-inflammatory effect of hypaphorine (HYP), nanoparticles with high bioavailability were prepared. VAC, HYP and chitosan nanoparticles (VAC + HYP-NPS) were used to the treatment of chronic wounds. Transmission electron microscopy (TEM) images showed the nanoparticles were spherical. ZetaPALS showed the potential of nanoparticles were −12.8 ± 5.53 mV and the size were 166.8 ± 29.95 nm. Methyl thiazolyl tetrazolium (MTT) assay showed that VAC + HYP-NPS had no toxicity and the biocompatibility was satisfactory. In the treatment of chronic wounds in diabetic rats, VAC + HYP-NPS significantly promoted the re-epithelialization of chronic wounds and accelerated the healing of chronic wounds. In the process of chronic wounds healing, VAC + HYP-NPS played the antibacterial effect of chitosan, the angiogenic effect of VAC and the anti-inflammatory effect of HYP, and finally promoted the chronic wounds healing. Overall, the developed VAC + HYP-NPS have potential application in chronic wounds healing. In view of the complexity of the causes of chronic wounds, multi-target drug administration may be an effective way to treat chronic wounds.

Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method. Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method. The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy. The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements. The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS. While the T g value of CS was 81 °C, this value increased by 13–94 °C with the addition of 6 wt% by weight of the ZnO-HSP. The antibacterial effect of materials was determined by the disc diffusion method. The ZnO-HSPs added to the CS caused the nanocomposites to have a remarkable effect against Escherichia coli and Staphylococcus aureus microorganisms. While the inhibition diameter of the CS against E. coli was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP. The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles. Thus, it was determined that the CS/ZnO-HSP nanocomposites did not have any cytotoxicity in healthy human cells. The fact that the produced nanocomposites exhibit antibacterial activity and do not harm human cells shows that they can be a safe product for health. From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.

Evaluation of antimicrobial and antibiofilm properties of chitosan edible coating with plant extracts against Salmonella and E. coli isolated from chicken

This study aims to investigate the effect of chitosan edible coating with medicinal leaf extracts, their antibiofilm and antimicrobial activity against the selected strains of Salmonella spp. and E. coli isolated from the chicken sample. The presence of bioactive compounds in leaf extracts was confirmed by antioxidant assay, polyphenolic content, UV–VIS, FTIR, and GC–MS analysis. Chitosan with M. piperita and P. amboinicus (ECMO) had a greater zone of inhibition against the multidrug-resistant strains (Salmonella spp., 22.10 ± 1.15; E. coli, 24.50 ± 0.500) and biofilm inhibition percentage was ≤96% in both Salmonella spp. and E. coli. In shelf-life analysis, ECMO significantly lowered the growth of pathogens during 15 days of storage period. The results suggest that ECMO was identified as the impeding agent for biofilms and foodborne pathogens. To our knowledge, this is the first study demonstrating the antibiofilm and antibacterial properties of chitosan with M. piperita and P. amboinicus. Novelty impact statement The combination of chitosan with M. piperita and P. amboinicus is the first study on chicken that significantly controlled pathogens during storage period at 4°C. Antibiofilm property was exhibited by chitosan with M. piperita and P. amboinicus against Salmonella spp. and E. coli. Chitosan with M. piperita and P. amboinicus, perchance a new natural preservative for perishable foods.

"Preparation, physico-chemical characterization and antibacterial properties of chitosan and chitosan–nisin membranes "

"Preparation, physico-chemical characterization and antibacterial properties of chitosan and chitosan–nisin membranes "

Two birds with one stone: Interfacial controls and pH response for long-term and high-efficiency Cu2O antibacterial materials

Cu2O is the current mainstream antifouling agent, but it has problems such as explosive release of Cu2+ ions, poor sterilization effect, and environmental pollution. In this work, we used the weakly acidic solution generated during the preparation of Cu2O@Ag to dissolve chitosan (CS) and then prepared Cu2O@Ag@CS core–shell nanocomposites using a one-pot method. The emergence of Cu2O@Ag@CS composites solved the above-mentioned problems of Cu2O. The CS shell can protect the Cu2O well, avoid direct contact with the outside environment, and reduce the oxidation of Cu2O. In addition, the CS shell provided a pH-response performance, and made Cu2O crystal face more active. The acidic environment generated by bacteria and other microorganisms opened the CS shell, causing Cu2O@Ag@CS to release a large amount of Cu2+ ions. In a neutral or alkaline environment, the release of Cu2+ ions were less, greatly improving the utilization efficiency of Cu2+ ions. Ag nanoparticles deposited onto the surface of Cu2O further protected Cu2O from oxidation, significantly improving the photocatalytic efficiency of Cu2O and enhancing its sterilization efficiency. The antibacterial properties of CS itself with more holes also benefited the improvement in the sterilization effect of Cu2O@Ag@CS. A 30-day long-acting antibacterial experiment proved the long-acting and high-efficiency antibacterial properties of Cu2O@Ag@CS composites. After the antifouling coating with Cu2O@Ag@CS as an antifouling agent was hung in a marine environment for 70 days in summer, no microorganisms attached onto the surface, proving the excellent practical application performance of Cu2O@Ag@CS antifouling agent.

Antibacterial property of chitosan against E. faecalis standard strain and clinical isolates.

To investigate applications of chitosan as antibacterial agent for endodontic treatments, we tested its activity against Enterococcus faecalis standard strain (ATCC29212) and clinical isolates. We determined the minimum bactericidal concentration (MBC) of 6 types of chitosan against ATCC29212; the most effective types were selected for further tests. Four clinical isolates were cultured from endodontically treated-teeth and identified by biochemical assays and polymerase chain reactions. Bacterial cultures were exposed to 1,700 and 2,100 kDa chitosan at MBC for 1, 3, 5, 10, and 60 min in time-kill assays and plated on brain-heart-infusion (BHI) agar for colony counts. Both types of chitosan showed significantly lower numbers of remaining bacteria (log colony forming units per millimeter, logCFUs/mL) than negative controls (0.1% acetic acid and BHI) at 10 min, and completely eliminated the bacteria at 60 min for all strains. Thus, chitosan could be developed as alternative biocompatible antimicrobial irrigant/medication for endodontic treatments.

Biocompatible AIE material from natural resources: Chitosan and its multifunctional applications

New sources of AIE materials with good water solubility, biocompatibility, degradability, and mass production are urgently needed. Here, we found that chitosan, a very abundant polysaccharide in nature, has fluorescence emission in both solid and solution states with AIE effect, and explored its multifunctional applications. Chitosan can emit a variety of colors from blue to red at different excitation wavelengths with excellent multicolor imaging capabilities at the cellular level. Utilizing the cationic and antibacterial properties of chitosan, the quantification of bacteria can be achieved through the AIE effect. Concurrently, it can be used as fluorescent probes for multi-channel bacterial imaging via lighting-up bacteria. Furthermore, the chitosan solution exhibits a sensitive quenching response to Fe3+, which can be used as a biosensor for detecting the concentration of Fe3+. These interesting results indicate that chitosan will have broad application prospects as a new class of AIE material.

Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.

To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo). Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films. The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined. The results confirmed all of the films exhibited excellent UV protection with low transparency at 600nm. Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength. SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM. Additionally, the incorporation of GE and CEo to the films enhanced their thermal stability and contact angle, but decreased their crystallinity and wettability. The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%. The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06µg/mL, respectively. These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising. PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable. The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials. CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.

Preparation of Gallic Acid-Grafted Chitosan Using Recombinant Bacterial Laccase and Its Application in Chilled Meat Preservation.

To improve the antibacterial and antioxidant properties of chitosan (CS), CS grafted with gallic acid (GA) using recombinant bacterial laccase from Bacillus vallismortis fmb-103 (fmb-rL103) as a catalyst. The structures of grafted chitosans were identified using Fourier transform infrared spectroscopy (FT-IR) and UV visible spectrum (UV–Vis spectroscopy). After gallic acid grafting, the antibacterial properties of chitosans against Pseudomonas, Acinetobacter, Brochothrix thermosphacta, Escherichia coli, Staphylococcus aureus, Salmonella, and Listeria monocytogenes were significantly improved. Meanwhile, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging results showed that the antioxidant properties of grafted CS increased as well. The preservative effects of the grafted chitosan on chilled meat were then investigated. For this purpose, the quality indexes of the chilled meat during the storage were monitored, including total bacterial count, total basic volatile nitrogen (TVB-N) content, pH value, color and thiobarbituric acid reactive substances (TBARS) and so on. The results showed that coating with the grafted chitosan retarded the growth of spoilage bacteria, and decreased TVB-N and TBARS values of meat. The shelf life of chilled meat coated by CS grafted with GA (GA-g-CS) also extended from 6 days to 18 days at 4°C. These results provided a theoretical basis for the future application of the GA-g-CS in the preservation of chilled meat.Highlights: (1)The temperature and pH-stable bacterial laccase was used to synthesize gallic acid grafted chitosan.(2)Antioxidant and antibacterial properties of chitosan were improved through grafting gallic acid.(3)Storage properties of chilled meat were improved by coating with gallic acid grafted chitosan.

Antibacterial Performance of PCL-Chitosan Core-Shell Scaffolds.

In this study, antibacterial performance of the coaxially electrospun Poly-ε-caprolactone (PCL)-chitosan core-shell scaffolds developed, optimized and identified physically and chemically in our previous study, were evaluated for the suitability in wound healing applications. The aim of utilizing a core-shell fibrous scaffold with PCL as core and chitosan as shell was to combine natural biocompatibility, biodegradability and antibacterial properties of chitosan with mechanical properties and resistance to enzymatic degradation of PCL. The scaffolds were prepared with the optimized parameters, obtained from our previous study. Thickness and contact angle measurements as well as Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analyses confirmed repeated fabrication of PCL-chitosan core-shell scaffolds. In this study, assays specific to wound dressing materials, such as water vapor transmission rate (WVTR), in vitro degradability and antibacterial tests were carried out. WVTR value of PCL-chitosan core-shell scaffolds was higher (2315 ± 3.4 g/m2 · day) compared to single PCL scaffolds (1654 ± 3.2 g/m2 · day) due to the higher inter-fiber pore size. Additionally, in vitro degradability assays showed that the susceptibility of chitosan to enzymatic degradation can be significantly improved by hybridization with more resistant PCL while still keeping the scaffold to be considered as biodegradable. Finally, inhibition ratio and inhibition zone measurements showed that the PCL-chitosan core-shell polymeric scaffolds had significant antibacterial performance (52.860 ± 2.298% and 49.333 ± 0.719% inhibition ratios; 13.975 ± 0.124 mm and 12.117 ± 0.133 mm clear inhibition zones, against E. coli and S. aureus, respectively), close to the native chitosan. Therefore, the developed scaffolds can be considered as suitable candidates for biodegradable wound dressing applications.

Preparation of ferulic acid-grafted chitosan using recombinant bacterial laccase and its application in mango preservation.

To improve the antioxidant and antibacterial properties of chitosan, recombinant bacterial laccase from Bacillus vallismortis fmb-103 (fmb-rL103) was used to catalyze ferulic acid grafting. The grafted chitosan was characterized using UV-vis and FT-IR techniques. DPPH free radical scavenging results indicated that the antioxidant properties of the grafted chitosan (FA-g-CS) were significantly improved. Meanwhile, the antibacterial properties against E. coli, S. aureus, B. subtilis, and M. guilliermondii were also improved. Furthermore, FA-g-CS was applied to mango preservation as a coating, which improved the sensory qualities of mango Mangifera indica L. The disease incidence of mangoes coated with FA-grafted medium and high molecular weight chitosan were 0 and 5%, respectively. The respiratory peak was delayed more than 4 days, and the titratable acidity and ascorbic acid concentration were all enhanced. POD and CAT activities in FA-g-CS coated mangoes were higher than those coated with chitosan samples with a lower H2O2 concentration.

Processing and antibacterial properties of chitosan-coated alginate fibers

The preparation of chitosan-coated alginate fibers by a wet spin process is presented and the characterization of the antibacterial activities of these fibers is discussed. Preformed calcium alginate fibers were passed in chitosan acetate solutions. The coagulation method of the coating consisted in the immersion of fibers in a bath of calcium dihydroxide solution (0.1 M). The antibacterial evaluation was achieved by a CFU (Colony-Forming Units) counting method after 6 h of incubation at 37 °C. The incorporation of chitosan on calcium alginate fibers brings antibacterial activities against Staphylococcus epidermidis, Escherichia coli and various Staphylococcus aureus strains namely MSSA (Methicillin Sensitive Staphylococcus aureus), CA-MRSA (Community Associated Methicillin Resistant Staphylococcus aureus) and HA-MRSA (Healthcare Associated Methicillin Resistant Staphylococcus aureus) which make these chitosan-coated fibers potential candidates for wound dressing materials. Developing a wound dressing with the haemostatic and healing properties of alginate combined with antibacterial properties of chitosan is envisioned for fighting against the infections and more particularly nosocomial diseases.

One-Pot Facile Methodology to Synthesize Chitosan-ZnO-Graphene Oxide Hybrid Composites for Better Dye Adsorption and Antibacterial Activity.

Novel chitosan–ZnO–graphene oxide hybrid composites were prepared using a one-pot chemical strategy, and their dye adsorption characteristics and antibacterial activity were demonstrated. The prepared chitosan and the hybrids such as chitosan–ZnO and chitosan–ZnO–graphene oxide were characterized by UV-Vis absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy. The thermal and mechanical properties indicate a significant improvement over chitosan in the hybrid composites. Dye adsorption experiments were carried out using methylene blue and chromium complex as model pollutants with the function of dye concentration. The antibacterial properties of chitosan and the hybrids were tested against Gram-positive and Gram-negative bacterial species, which revealed minimum inhibitory concentrations (MICs) of 0.1 µg/mL.

Technology for Obtaining and Using Granulated Absorbents Based on Chitosan

A method for obtaining granulated absorbents from chitosan is presented. They are shown to be efficient for purifying wastewater from heavy-metal ions. IR spectra of the obtained absorbents are given. Processing schemes for obtaining granulated absorbents based on chitosan are developed. The antibacterial properties of chitosan are studied.

Tailoring mechanical and antibacterial properties of chitosan/gelatin nanofiber membranes with Fe3O4 nanoparticles for potential wound dressing application

In this work, magnetic Fe3O4 nanoparticles (NPs) were utilized to improve the mechanical and antibacterial properties of chitosan (CS)/gelatin (GE) composite nanofiber membranes. Homogeneous Fe3O4/CS/GE nanofibers were electrospun successfully. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images confirmed the presence of well-dispersed Fe3O4 NPs in the composite nanofibers. Fourier transform infrared spectroscopy (FTIR) spectra revealed the effective interactions of Fe3O4 NPs to the composite matrix through hydrogen bonding. The improvement on the thermal stability of the Fe3O4/CS/GE was observed by differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA), which is tightly correlated to strong filler-matrix adhesion. The incorporation of Fe3O4 NPs resulted in a substantial enhancement of mechanical properties. The optimum mechanical performance was demonstrated on 1wt% Fe3O4/CS/GE nanofiber membranes, achieving 155% augment of Young's modulus, 128% increase of tensile strength, and 100% boost of toughness from CS/GE. The excellent mechanical enhancement can be explained by the effective dispersion of fillers and the filler-matrix interactions, which ensures the efficient load transfer from CS/GE matrix to Fe3O4 nanofillers. Moreover, zones of inhibition for Escherichia coli and Staphylococcus aureus expanded markedly with the supplement of Fe3O4 NPs. In all, nanofiber membranes made of Fe3O4/CS/GE composite with tailored mechanical and antibacterial properties appear a promising wound dressing material.