Lignin, a valuable biomaterial having an array of exciting properties is increasingly favoured as a reinforcement material in the fabrication of green composites. Reinforcement in biopolymers like natural rubber (NR) using lignin nanoparticles (LNP) is considered a hotspot today. In this study, LNP synthesized via the homogenization method was incorporated into natural rubber latex (NRL) using probe sonication, and NR/Lignin nanocomposites (NR/LNP) were fabricated using latex dipping method. The addition of LNP resulted in significant enhancements in mechanical and antibacterial properties, biodegradability, and ultraviolet (UV) blocking capabilities with the addition of 7 parts per hundred rubber (phr) of LNP, due to the uniform dispersion and effective interaction between NR and LNP. This research demonstrates a versatile pathway for integrating LNP into NR through a green method, enabling the production of eco-friendly NR nanocomposites for multifunctional applications. This pathway contributes to a safe disposal of NR based products.