Anti-single Stranded DNA Antibodies Research Articles

Polyclonal activation of lymphocytes induced in the mouse by acebutolol, a beta blocking agent

Acebutolol, a beta blocking agent, was injected at a dose of 1 mg/day, every day for 6 months in C57B1/6 and OF1 mice. No antinuclear antibodies were induced but a transient induction of anti-single stranded DNA antibodies and an increase of IgM, IgA and IgG2a levels were observed during the first 3 weeks in some treated mice as compared to control mice. This effect was more pronounced in C57B1/6 than in OF1 mice and in the C57B1/6 mice, was more frequently observed in female than in male mice. It was further found by cellular studies done in C57B1/6 female mice, that daily i.p. or oral administration of Acebutolol induces a transient polyclonal stimulation of lymphocytes. It is concluded that certain beta blockers might possess some in vivo effects on the immune system.

Evaluation of circulating immune complexes in leprosy in relation to clinical activity, antileprosy agents, bacterial indices and anti-DNA antibodies

Circulating immune complexes (CIC) were detected by Clq solid phase assay in 120 Japanese patients with leprosy and evaluated in relation to clinical activity, antileprosy agents, bacterial indexes and anti DNA antibodies.Following results were obtained ;1) The mean level of CIC in lepromatous, borderline and tuberculoid leprosy was high in comparison with those of control (Fig. 1.).2) In lepromatous leprosy, the positivity and mean level of CIC had the tendency to increase with activity of clinical stage. Statistical analysis revealed the significant difference between p & r1 and r3 & q groups (0.02<P<0.05) but no significant difference between p & r1 and r2 (Fig. 1).3) The incidence of CIC in B. I. (0) was 38.1% and that in B. I. (0.1-1.9) was 30.6%. The incidence in BI (2.0-2.9) and in BI (over 3.0) groups were relatively high, compared with BI (0) group. The mean CIC level of each BI varied almost parallel with the positivity of CIC. Maximum level of CIC was observed in BI (2.0-2.9), which was significantly different from BI (0) (p<0.01) (Fig. 2 and Table 1).4) The effects of antileprosy agents on CIC level in inactive patients with lepromatous leprosy were studied but there were no significant differences in CIC level (Fig. 3).5) Anti DNA antibodies, that is, anti single stranded DNA antibody, anti double-stranded DNA antibody and anti extractable nuclear antigen (ENA) antibody, were determined by radioimmunoassay in lepromatous and tuberculoid leprosy. A significant increase of anti ENA antibodies was found (Fig. 4).6) Correlation study between CIC and appearance of some anti DNA antibodies suggested anti DNA antibodies dib not play the significant role in the pathogenesis of leprosy (Table 2 and Table 3).