Antioxidant Activity Assay Research Articles

Green ultrasonic-assisted enzymatic extraction of polysaccharides from Flammulina velutipes residues by response surface methodology

Flammulina velutipes residues, the by-products of Flammulina velutipes industry, cause environmental pollution. The aim of the current work was to evaluate the extraction efficiency of polysaccharides from Flammulina velutipes residues by an ultrasonic assisted snailase enzymatic extraction (UAEE) method, following the principles of green chemistry, mainly solvent and energy saving. The optimized parameters obtained by Response Surface Methodology (RSM) were determined as follows: ultrasonic power at 150 W, extraction time of 90 min, and extraction temperature of 55 °C. The experimental extraction yield (109.54 ± 5.44 mg/g) was closely matched the predicted yield (110.92 mg/g). Polysaccharides from Flammulina velutipes residues (UE-FVRP) had the molecular weight of 170 kDa and a composition comprising 8 monosaccharides, primarily xylose (Xyl, 24.71%), mannose (Man, 17.29%), arabinose (Ara, 11.48%), and galactose (Gal, 15.63%). FT-IR analysis suggested that UE-FVRP likely contained pyranose with α-glycoside bonds. In vitro antioxidant activity assays revealed that UE-FVRP displayed potent DPPH radical scavenging activity and reducing ability. Additionally, UE-FVRP demonstrated the ability to promote the growth of two Lactobacillus strains, indicating its potential as a novel polysaccharide with high in vitro prebiotic properties. Therefore, these findings proposed a novel, green, and efficient extraction method for extracting polysaccharides from Flammulina velutipes residues, highlighting the potential of utilizing Flammulina velutipes residues as a valuable ingredient in both food and feed applications, contributing to the sustainable reuse of agricultural by-products.

Effects of combined treatment with hydrogen-rich electrolyzed water and tea polyphenols on oxidative stress, intestinal injury and intestinal flora disruption in heat-stressed mice

Heat stress (HS) can cause damage to the organism, especially the intestinal tract. In this paper, we investigated the effects of the combined action of tea polyphenols (TP) and hydrogen-rich electrolyzed water (HRW) on HS in mice. The combination of HRW feeding and TP of intraperitoneal injection was screened by in vitro antioxidant activity assay. The results revealed that the combined treatment was more helpful in alleviating the effects of HS on the behavior, growth performance, oxidative damage, and intestinal tract of mice compared with the respective treatments of TP and HRW (P < 0.05). Additionally, the combined treatment could repair HS-induced intestinal dysbiosis in mice, augmenting the number and abundance of bacteria, increasing the number of beneficial genera (Lachnospiraceae_NK4A136_group and Lactobacillus), and decreasing the number of harmful genera (Desulfovibrio and Enterorhabdus), and the effect was significantly better than that of individual treatment (P < 0.05). In conclusion, the combined treatment of TP and HRW effectively mitigates the adverse effects of HS on mouse behavior, growth performance, oxidative damage, and intestinal dysbiosis, surpassing the efficacy of individual treatments with TP or HRW alone.

Effect of Curcuma xanthorrhiza Extracts on the Phytochemical Content, Antioxidant Activity and Lactobacillus casei Growth

Curcuma xanthorrhiza (CX) has been reported as the most widely cultivated species in Southeast Asia. It is commonly found in the wild and has been traditionally used for medicinal purposes. The rhizome and root of CX contain constituents that offer various health benefits, including the treatment of skin inflammations and acne, as well as having antioxidant properties. Recently, numerous herbal products and traditional medicines made from CX have appeared in the market, available in forms such as capsules and bottled drinks. However, no research has yet validated the potential of growing Lactobacillus casei (L. casei) probiotic bacteria in CX extracts. The aim of this study is to determine the total phytochemical content of CX extracts, the antioxidant activity of CX extracts, and the prebiotic potential of CX. In this article, the chemical contents of CX, including total phenolics, total flavonoids, and antioxidant activity, have been studied using the Folin-Ciocalteu method, the aluminum chloride colorimetric method, and the DPPH free radical scavenging assay for antioxidant activity. The studies were conducted using three different methods of extracting CX: methanol-maceration, fresh juiced, and fresh boiled CX. The prebiotic potential of CX was studied using the L. casei probiotic strain. From the methanol-maceration extraction, the values of TPC and TFC are 111.55 ± 13.64 μg GAE/mg DW and 27.30 ± 7.75 μg QE/mg DW, respectively. Meanwhile, for the fresh juiced extraction, the TPC and TFC values are 30.513 ± 0.917 μg GAE/mg DW and 43.81 ± 5.13 μg QE/mg DW, respectively. Lastly, the boiled extraction method yields a TPC of 10.85 ± 0.21 μg GAE/mg DW and a TFC of 8.00 ± 0.49 μg QE/mg DW. At a concentration of 2000 μg/mL, the scavenging activity of the fresh juiced extraction reached a plateau of 89.33%. Meanwhile, at the same concentration, the scavenging effects of CX fresh methanol maceration and fresh boiled extraction are 61.39% and 49.81%, respectively. The L. casei strain was shown to be actively growing in CX extracts within the first 24 hours; however, after 24 hours, the growth remained almost constant. This may be due to the limited supply of CX extracts for the growth of the probiotic L. casei strain. Altogether, these results demonstrate that CX is a potential source of valuable bioactive compounds that could be used in medical, pharmaceutical, and food industries.

Revealing the Phenolic Composition and the Antioxidant, Antimicrobial and Antiproliferative Activities of Two Euphrasia sp. Extracts.

The species of the genus Euphrasia present important medicinal potential according to their traditional uses. However, few studies aim to sustain this fact by scientific evidence. The present study aimed to explore the phytochemical profile and investigate the antioxidant, antimicrobial and antiproliferative potential of E. officinalis subsp. pratensis Fr. (EO) and E. stricta J.P.Wolff ex J.F.Lehm (ES). The tested samples consisted of ethanolic extracts. The identification and quantification of phenolic compounds were performed using spectrophotometric and LC-MS/MS methods. The antioxidant capacity was evaluated using the DPPH, FRAP and xanthine oxidase methods. Antimicrobial properties were screened using disk diffusion, broth microdilution and anti-biofilm assays, while antiproliferative potential was assessed on a colorectal adenocarcinoma human cancer cell line (DLD-1). The LC-MS/MS analysis showed chlorogenic acid and rutin as the dominant constituents in the tested extracts. The antioxidant activity assays showed important capacity for both samples; in vitro antimicrobial and anti-biofilm properties were exhibited, especially against Gram-positive bacteria, and an important inhibitory potential was observed on the proliferation of the DLD-1 cell line. The findings in the present study contribute to the recommendation of EO and ES for the prevention and treatment of oxidative stress-related pathologies, cancer and microbial infections.

The study on in vitro antioxidant properties, cellular immune activities and mice anti-fatigue effects of Sanghuangporus vanninii mycelium polysaccharides

Sanghuang mycelial polysaccharides (SVMPs) were studied using poplar sanghuang as raw material (sanghuangporus vanninii, SV). The bioactivity of SVMPs was evaluated through in vitro antioxidant and cellular immunity activity assays, and the acute oral toxicity and anti-fatigue ability of SVMPs in mice were determined using mice as an experimental animal model. The results showed that SVMPs have a good scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+) radicals, with a slightly weaker reducing power. SVMPs promote, to some extent, the secretion of cytokines Tumor Necrosis Factor-α (TNF-α), Interleukin-1β (IL-1β), and Interleukin-6 (IL-6), and the release of NO from mouse macrophage RAW264.7. In mouse experiments, the mouse tolerance of SVMPs was more greater than 18.2 g/kg BW, which was 2724 times the daily dosage of humans, and it was a non-toxic substance; the high dose group of SVMPs could significantly prolong the swimming time of weight-bearing mice. Thus, the results of this study clearly demonstrate the biological activity of SVMPs and provide a valuable basis for the potential application of SVMPs as antioxidants, immunomodulators, and functional foods.

Evaluation of the synergistic potential of Camellia sinensis and Badri Cow urine through UV-shielding assay and estimation of antioxidant and antibiofilm activity

Evaluation of the synergistic potential of Camellia sinensis and Badri Cow urine through UV-shielding assay and estimation of antioxidant and antibiofilm activity

Comparison of maceration and infundation towards antioxidant capacity of leaves aqueous extracts of balik angin (Alphitonia incana (Roxb.) Teijsm. &amp; Binn. ex Kurz)

Background: Excessive exposure to free radicals can trigger various degenerative diseases. Therefore, external antioxidants are needed from nature. One plant with the potential as a natural antioxidant is Balik Angin (Alphitonia incana (Roxb.) Teijsm. &amp; Binn. Ex Kurz). Objective: This study aimed to determine the differences in the content of secondary metabolites and the antioxidant capacity of the aqueous extract of balik angin leaves from two different extractions. Method: The extraction methods were maceration and infundation, followed by phytochemical screening to identify secondary metabolites. The antioxidant activity assay was carried out by UV-Vis spectrophotometry by measuring the absorbance of the sample reaction with CUPRAC and FRAP reagents. Result: The phytochemical screening results of balik angin leaves infusion contained phenols, flavonoids, saponins, triterpenoids, and tannins, while the macerated extract obtained triterpenoids and tannins negatively. The quantitative assay showed the balik angin leaves infusion resulted in a powerful antioxidant category with EC50 values of 17.762 µg/mL and highly antioxidant content, which was 726 mg AAE/g extract compared to macerated aqueous extract antioxidant capacity with EC50 values of 44.719 µg/mL and 635 mg AAE/g extract antioxidant content. Conclusion: The infused aqueous extract of balik angin leaves has the potential to be a powerful antioxidant compared to the maceration product.

Alginate – Gamat Gel Hydrogel Films as the Potential Wound Dressing

Alginate is a biopolymer that is biodegradable, non-antigenic, non-toxic, and has high biocompatibility. Gamat gels of sea cucumber Stichopus horrens are used to treat pain, wounds, and other ailments. Victims suffering from burn wounds frequently complain of pain and discomfort while being bandaged. This study aims to analyze the characteristics of alginate as the base biomaterial for hydrogel films and its interaction with gamat gels bioactive components. Antioxidant activity assay was done to the gamat gels using DPPH assay. Hydrogel films were prepared with different alginate concentrations of 1% w/v, 3% w/v, and 5% w/v. Film degradation was analyzed using stereo microscope. Mechanical characteristics were evaluated based on tensile strength, brittleness, elasticity, adhesion capacity, and the ease when handling the films. Hydrolytic stability was evaluated based on swelling capacity and matrix stability. Antibacterial activity assay was carried out using disk diffusion on Staphylococcus aureus. Results show that gamat gels have DPPH inhibition capacity of IC50 = 2937.5 ppm. The alginate concentrations treatment resulted in films with different morphology. Films with 5% w/v alginate concentration exhibit a rough surface, the better tensile strength, capability to conform to various surfaces, good adhesion (average detachment time of 60 seconds), swelling capacity of 11,96 ± 13,80%, and matrix stability with the average weight loss of 33,26%. FTIR spectroscopy confirmed that alginate was able to bond with Ca2+ ions and the gamat gels. Gamat gels show inhibition zone of ≤ 5 mm. Films with 5% w/v alginate concentration were able to release bioactive compounds.

Synthesis, Characterization, and Cytotoxicity Evaluations of Silver–Zeolite Nanocomposite

Zeolites of natural origin are materials exhibiting many positive effects on the human body. Silver-modified zeolites have already been introduced as bactericidal agents, although studies dealing with their toxicity are insufficient. This work describes the synthesis of activated and silver-loaded Bulgarian zeolite using a simple wet impregnation method. Morphological characteristics and compositions of natural zeolite, activated zeolite, and Ag-nanocomposites were studied by the X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) methods. Silver loading is approximately 13 wt. %, with mean Ag particle size around 19 nm. Analyses of the samples included antioxidant activity assays based on ABTS radical scavenging ability and in vitro cytotoxicity tests with human normal fibroblasts and three adenocarcinoma cell lines. The experiments were performed with natural, activated, and Ag-modified zeolite in comparison to two commercial food supplements. Our results indicated moderate antioxidant activity of the tested samples. Silver-modified zeolite demonstrated cytotoxic effects against both tumor cells and normal fibroblasts, but the detected levels of inhibition were stronger against the adenocarcinoma cells, suggesting anti-tumor potential. The present article indicates a new aspect of Bulgarian natural zeolite and Ag-loaded zeolite biological activity. It highlights the need for detailed toxicity evaluations of Ag-nanocomposites prior to healthcare applications.

INVESTIGATION OF ANTIOXIDANT ACTIVITIES OF Cenchrus purpureus EXTRACTS USING In Vitro ASSAY

This in vitro study evaluated Cenchrus purpureus (also called as Napier grass, elephant grass) extract’s antioxidant capacity and Phytoactive compounds. At present various parts of plant materials are becoming more popular for their health advantages as well as natural antioxidants. Ethanolic, methanolic, and aqueous extracts were examined to find out the total phenolic compound (TPC) (138.54±1.66, 77.5±1.73, 53.63±2.05 mg GAE/g), total flavonoids compound (TFC) (34.49±1.57, 28.24±3.35, 16.58±1.41 mg QE/g) of dry extract respectively, as well as antioxidant activity assay including DPPH scavenging capacity using C. purpureus extract. The ethanol extract of the three had an IC50 value of 39.41 µg/mL, similar to conventional ascorbic acid (31.46 µg/mL), suggesting its potential as a natural antioxidant. The study highlights the importance of examining diverse plant species to discover bioactive compounds that can be used to create innovative antioxidant agents for pharmaceuticals and functional food products.

Synthesis, structure of the N-(Alkyl/Arylsulfonyl) substituted 5-(Bromo/Iodo)-3-methylindazoles and bioactivity screening against some of the biochemical targets linked to type 2 diabetes mellitus

The indazole scaffold and sulfonamide moiety have evoked high interest in medicinal chemistry especially in the context of antidiabetic drug development. A series of the C-5 unsubstituted (2a–f), 5‑bromo (2g–l) and 5-iodo (2m–r) substituted 1-(alkyl/arylsulfonyl)-3-methylindazoles were prepared, characterized and then evaluated through enzymatic assay in vitro for anti-α-glucosidase activity and for potential free radical scavenging properties. Compounds 2b, 2d, 2f–h, 2k, 2p and 2q exhibited strong activity against α-glucosidase compared to acarbose (IC50 = 1.30 ± 0.02 µM) with IC50 values in the range of 0.72 ± 0.03 – 1.20 ± 0.01 µM. These compounds exhibited increased or strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and/or nitric oxide (NO) free radical scavenging activities. The compounds were, in turn, evaluated through enzymatic assays in vitro for the potential to inhibit or activate superoxide dismutase (SOD), lipoxygenase-15 (LOX-15) and/or vascular endothelial growth factor receptor-2 (VEGFR-2) activities. An in vitro cell-based antioxidant activity assay involving lipopolysaccharide induced reactive oxygen species (ROS) production in the human breast (MCF-7) cancer cell line revealed that compounds 2d, 2f and 2h exhibit moderate activity (35.5 %, 47 % and 28 %, respectively) compared to staurosporine (24 %). Compounds 2d, 2f and 2h significantly reduced the ROS level in the human embryonic kidney (HEK293-T) cells (65.5 %, 58 % and 48 %, respectively) compared to staurosporine (34 %). The planar benzo-fused heterocyclic scaffold and the distorted tetrahedral geometry of the N-sulfonyl moiety are predicted to facilitate hydrophobic and/or hydrogen bonding interactions to stabilize protein-ligand interaction in the receptors. The ADME analysis predicted that the test compounds have drug-like properties, a safe toxicity profile in silico without any carcinogenic and mutagenicity side effect. Molecular modelling was also used to attempt to rationalize the biological activity of these compounds and to correlate the results of in vitro and in silico studies.

Assessment of the Antioxidant and Hypolipidemic Properties of Salicornia europaea for the Prevention of TAFLD in Rats.

Halophyte species represent valuable reservoirs of natural antioxidants, and, among these, Salicornia europaea stands out as a promising edible plant. In this study, young and old S. europaea leaves were compared for the content of bioactive compounds and antioxidant activity to assess changes in different growth phases; then, the potential protective effects against low-dose CCl4-induced toxicant-associated fatty liver disease (TAFLD) were investigated by administering an aqueous suspension of young leaves to rats daily for two weeks. Quantification of total and individual phenolic compounds and in vitro antioxidant activity assays (DPPH, FRAP, and ORAC) showed the highest values in young leaves compared to mature ones. Salicornia treatment mitigated CCl4-induced hepatic oxidative stress, reducing lipid peroxidation and protein carbonyl levels, and preserving the decrease in glutathione levels. Electronic paramagnetic resonance (EPR) spectroscopy confirmed these results in the liver and evidenced free radicals increase prevention in the brain. Salicornia treatment also attenuated enzymatic disruptions in the liver's drug metabolizing system and Nrf2-dependent antioxidant enzymes. Furthermore, histopathological examination revealed reduced hepatic lipid accumulation and inflammation. Overall, this study highlights Salicornia's potential as a source of bioactive compounds with effective hepatoprotective properties capable to prevent TAFLD.

Compositional, structural, and functional characterization of fucoidan extracted from Sargassum polycystum collected from Saint Martin's Island, Bangladesh

Fucoidan is a sulphated polysaccharide with diversified biological activities commonly found in brown seaweeds. In our present study, fucoidan was extracted from Sargassum polycystum collected from Saint Martin's Island in three different extraction methods. The composition of the fucoidans was determined by measuring the total carbohydrate, sulfate, uronic acid, fucose, and protein contents. Fourier-transform infrared spectroscopy (FT-IR) and Proton nuclear magnetic resonance (1H NMR) were studied to determine the structural groups of extracted fucoidans. Functional activities were analyzed by antioxidant activity and anti-diabetic activity assay. Fucoidan yield was obtained highest in the acid extraction method (0.061 ± 0.007 g/g), followed by the water extraction method (0.058 ± 0.005 g/g) and salt extraction method (0.041 ± 0.005 g/g). Water extracted fucoidan showed the highest carbohydrate content (62.97 %), uronic acid content (6.46 %), and protein content (2.62 %). Sulphate content (28.56 %) was highest in salt extracted fucoidan, whereas fucose content (24.0 %) was highest in water extracted fucoidan. FT-IR and 1H NMR analysis confirm several characteristic picks for the extracted fucoidans. Extracted fucoidans presented comparable antioxidant activity, but salt-extracted fucoidan presented the highest DPPH radical scavenging assay (86.85 ± 0.74 %, IC50 = 0.03 mg/mL), ABTS radical scavenging assay (65.13 ± 0.92 %, IC50 = 1.94 mg/mL), Phosphomolybdenum assay (4.356 ± 0.11) and Nitric Oxide (NO) radical scavenging assay (57.08 ± 0.52 %, IC50 = 3.73 mg/mL) in dose-dependency manner. Further anti-diabetic activity assay resulted in the highest α-amylase inhibition activity (62.82 ± 1.36 %, IC50 = 2.30 mg/mL) and α-glucosidase inhibition activity (55.73 ± 0.84 %, IC50 = 3.71 mg/mL) by salt-extracted fucoidan. These functional potentials of seaweed may add significant value to the development of functional food and pharmaceutical materials.

Phenolic Compounds Profile and Antioxidant Capacity of Plant-Based Protein Supplements.

The study aimed to determine the phenolic content and antioxidant capacity of five protein supplements of plant origin. The content and profile of phenolics were determined using the UHPLC-DAD-MS method, while antioxidant capacity (ABTS and DPPH assays) and total phenolic content (TPC) were evaluated using spectrophotometric tests. In the analyzed proteins, twenty-five polyphenols were detected, including eleven phenolic acids, thirteen flavonoids, and one ellagitannin. Hemp protein revealed the highest individual phenolics content and TPC value (1620 μg/g and 1.79 mg GAE/g, respectively). Also, hemp protein showed the highest antioxidant activity determined via ABTS (9.37 μmol TE/g) and DPPH (9.01 μmol TE/g) assays. The contents of p-coumaric acid, m-coumaric acid, kaempferol, rutin, isorhamnetin-3-O-rutinoside, kaempferol-3-O-rutinoside, and TPC value were significantly correlated with antioxidant activity assays. Our findings indicate that plant-based protein supplements are a valuable source of phenols and can also be used in research related to precision medicine, nutrigenetics, and nutrigenomics. This will benefit future health promotion and personalized nutrition in the prevention of chronic diseases.

NNO donor Schiff base metal complexes: Enzyme mimicking, DNA binding and biological insights

The present work focuses on the microwave-assisted synthesis, characterization and biological applications of a novel tridentate NNO donor Schiff base ligand derived from the condensation of 4-(Diethylamino)salicylaldehyde and 4,5-Dimethyl-1,2-phenylenediamine, along with its transition metal complexes of Cu(II) and Co(II) in 1:1 and 1:2 ratios each respectively. A theoretical basis for comprehending the electronic framework and characteristics of the complexes has been studied through the use of a computational approach. Further, the distorted octahedral geometry of the Schiff base metal complexes is confirmed by using analytical methods such as elemental analysis, molar conductance, ESI-MS, UV–Vis, FTIR, TGA and NMR spectra, which confirmed that NNO donor site of the ligand is coordinated with the metal ions. The complexes have been screened for their potential application as a catalyst for the catalytic conversion of 3,5-di‑tert‑butyl‑catechol (3,5-DTBC) to 3,5-di‑tert-butylquinone (3,5-DTBQ) and the turnover numbers (Kcat) have been found in the range 36.46–43.05 h−1 in methanol. Absorption spectroscopic technique has been employed to examine the possible modalities of interactions with the synthesized complexes using CT-DNA and the related binding constants exhibited in the range 2.58 × 104 M−1 to 1.98 × 105 M−1 thus depicting appreciable binding affinity. To evaluate the bioactivities, in-vitro assays for anti-oxidant and anti-inflammatory activity have also been implemented. The Cu(II) complex (CuL) has significant anti-oxidant activity and the Co(II) complex (CoL) has superior anti-inflammatory activity compared to all other complexes with IC50 values of 238.78 µM and 140.25 µM respectively. To better understand the molecular-level interactions and identify probable binding energies of inhibitors, molecular docking simulations have been performed.

Network pharmacology of Dracaena sp. in Guangxi and its related species leaf secondary metabolites possess antioxidant properties

In this study, metabolite maps were conducted on leaf samples accessions of Dracaena sp. in Guangxi (C6, C13, CZ4, JX2, RM1, and BM3) and six related species including D. angustifolia (CH), D. elliptica (XZ), D. cochinchinensis (CB), D. cambodiana (HN), D. marginata (HB), and Yucca schidigera (SL). We identified 2,971 compounds including carboxylic acids and their derivatives, organooxygen compounds, prenol lipids, benzenes and their substituted derivatives, and flavonoids. Of those, 73 characteristic secondary metabolites were screened out by weighted correlation network analysis (WGCNA). Pterostilbene, 9-cis-retinal, and dracorubin were unique to the Dracaena sp. in Guangxi, and 3-hydroxytridecanoic acid and chrysin were highly abundant in them. An in vitro antioxidant activity assay disclosed that all 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging rates and all fluorescence recovery after photobleaching (FRAP) rates were in the ranges of 75–87 % and 3.10–3.25 μmol/mL, respectively, for the Dracaena sp. in Guangxi. Hence, the latter have relatively high and stable foliar antioxidant activity. A network pharmacological analysis of the top 20 metabolites in Dracaena leaf and their 446 antioxidant-related disease targets revealed ten core action targets, including GAPDH, AKT1, MAPK3, VEGFA, CASP3, TNF, MAPK1, SRC, EGFR, and MAPK8. An antioxidant-related compound-target-pathway network was constructed and verified by molecular docking. The results of this study provide a theoretical basis for mining and exploiting the foliar secondary metabolites of Dracaena.

Changes in physicochemical and digestibility properties of foxtail millet prolamin after cooking, and novel antioxidant peptide identification from cooked foxtail millet prolamin

The presence of antioxidant peptides in cooked foxtail millet prolamin (CFMP) is pivotal for its ability to mitigate oxidative stress and subsequently exert an anti-diabetic effect. However, the alterations in structural characteristics and antioxidant activities of foxtail millet prolamin following cooking remain unclear. Furthermore, the identification of antioxidant peptides in CFMP targeting myeloperoxidase (MPO), a key enzyme implicated in oxidative stress, has yet to be achieved. This study aims to examine the changes in physicochemical, digestibility, and antioxidant properties of foxtail millet prolamin post-cooking, and to identify potential antioxidant peptides from CFMP targeting MPO. The findings revealed significant reductions in water and oil holding capacities, foaming and emulsifying capacities, surface hydrophobicity, and zeta potential of foxtail millet prolamin after cooking, along with a notable increase in average particle size, indicative of protein molecule aggregation and increased resistance to digestion. Three potential antioxidant peptides (IFVFPSGAG, MMFPM, and VLIPMP) were identified in CFMP hydrolysate. Molecular dynamic simulation demonstrated favorable binding interactions between all three peptides and MPO, primarily mediated by hydrogen bonds. In vitro antioxidant activity assays confirmed IFVFPSGAG as the most potent antioxidant peptide. This study sheds light on the properties of CFMP and the antioxidant potential of its derived peptides.

Physicochemical Characterization, Antioxidant and Anticancer Activity Evaluation of an Acidic Polysaccharide from Alpinia officinarum Hance.

AHP-3a, a triple-helix acidic polysaccharide isolated from Alpinia officinarum Hance, was evaluated for its anticancer and antioxidant activities. The physicochemical properties and structure of AHP-3a were investigated through gel permeation chromatography, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The weight-average molecular weight of AHP-3a was 484 kDa, with the molar percentages of GalA, Gal, Ara, Xyl, Rha, Glc, GlcA, and Fuc being 35.4%, 21.4%, 16.9%, 11.8%, 8.9%, 3.1%, 2.0%, and 0.5%, respectively. Based on the results of the monosaccharide composition analysis, methylation analysis, and NMR spectroscopy, the main chain of AHP-3a was presumed to consist of (1→4)-α-D-GalpA and (1→2)-α-L-Rhap residues, which is a pectic polysaccharide with homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) structural domains containing side chains. In addition, the results of the antioxidant activity assay revealed that the ability of AHP-3a to scavenge DPPH, ABTS, and OH free radicals increased with an increase in its concentration. Moreover, according to the results from the EdU, wound healing, and Transwell assays, AHP-3a can control the proliferation, migration, and invasion of HepG2 and Huh7 hepatocellular carcinoma cells without causing any damage to healthy cells. Thus, AHP-3a may be a natural antioxidant and anticancer component.

Antimicrobial secondary metabolites and antioxidant activities of fungal endophytes associated with Ziziphus spina-christi (L.) Desf. (Nabq) leaves

A total of 20 endophytic fungi were isolated (ZSEFL1-ZSEFL20) from Ziziphus spina-christi (L.) Desf. (Nabq) leaves. Four isolates A2/ZSEFL2, Alternaria alternata, D/ZSEFL14, Aspergillus niger, E/ZSEFL15, Epicoccum nigrum, and S/ZSEFL19, Penicillium crustosum were found to show the most promising antimicrobial activities either in plug or disc diffusion screening assays against Gram-positive, Gram-negative bacteria and pathogenic fungi. Antimicrobial activity was tested against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, Serratia marcescens ATCC 14764, Klebsiella pneumoniae ATCC 700603, Candida albicans ATCC 10231, and Fusarium oxysporum ATCC417. In vitro antioxidant activity assay was conducted using the ABTS [2,2′-Azino-bis (3-Ethylbenzthiazoline-6-Sulfonic Acid)] free radical scavenging method. EtOAc extracts of all isolated endophytic fungi showed antioxidant activities. This study would be one of the first reports to measure the antioxidant activity of Z. spina-christi (L.) Desf. endophytic fungi. Therefore, these isolated endophytic fungi can provide additional information for medicinal sources of natural antioxidants and antimicrobial agents.

Exploring the Chemical Profile, In Vitro Antioxidant and Anti-Inflammatory Activities of Santolina rosmarinifolia Extracts.

In this study, the phytochemical composition, in vitro antioxidant, and anti-inflammatory effects of the aqueous and 60% ethanolic (EtOH) extracts of Santolina rosmarinifolia leaf, flower, and root were examined. The antioxidant activity of S. rosmarinifolia extracts was determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The total phenolic content (TPC) of the extracts was measured by the Folin-Ciocalteu assay. The anti-inflammatory effect of the extracts was monitored by the Griess assay. The chemical composition of S. rosmarinifolia extracts was analysed using the LC-MS technique. According to our findings, 60% EtOH leaf extracts showed the highest Trolox equivalent antioxidant capacity (TEAC) values in both ABTS (8.39 ± 0.43 µM) and DPPH (6.71 ± 0.03 µM) antioxidant activity assays. The TPC values of the samples were in good correspondence with the antioxidant activity measurements and showed the highest gallic acid equivalent value (130.17 ± 0.01 µg/mL) in 60% EtOH leaf extracts. In addition, the 60% EtOH extracts of the leaves were revealed to possess the highest anti-inflammatory effect. The LC-MS analysis of S. rosmarinifolia extracts proved the presence of ascorbic acid, catalpol, chrysin, epigallocatechin, geraniol, isoquercitrin, and theanine, among others, for the first time. However, additional studies are needed to investigate the direct relationship between the chemical composition and physiological effects of the herb. The 60% EtOH extracts of S. rosmarinifolia leaves are potential new sources of natural antioxidants and anti-inflammatory molecules in the production of novel nutraceutical products.