Antinutritional Factors Research Articles

Characterizing sorghum genotypes for forage yield, hydrocyanic acid and sugar contents under arid climate conditions

AbstractSorghum (Sorghum bicolor (L.) Moench) is a dual nature crop, which is used for food as well as fodder, depending on plant ideotype. Sorghum forage is important for ruminants, but a major constraint is the anti-nutritional factor dhurrin, a hydrocyanic acid (HCN) producing glucoside. There are several additional effects of dhurrin, which reduce the nutritional value of sorghum fodder for livestock. This two-year study was aimed to evaluate the variation among diverse sorghum varieties, specifically for HCN content, forage yield and stem sugar content (brix value) under arid climate in Pakistan. Nine sorghum varieties were used for this experiment: JS-2002, Chakwal sorghum, Lines CS-17, Super late, PAK SS-2, Johar, JS-263, Sargodha-2011 and YSS-98. Results reveal that Sargodha-2011 had superior morphological traits for fresh forage and dry biomass yield, and stem brix value, compared to other varieties. Higher HCN contents were recorded in Super late compared to other varieties. Significant negative correlation of HCN with yield showed that improvement in yield will reduce the HCN content of sorghum. In conclusion, sorghum variety SGD-11 was shown best performing for higher biomass yield and brix value, and lower HCN content compared to other tested varieties under arid climate of Thal, Pakistan.

Evaluating the impact of organic chromium on hepatic phospholipid fatty acid molecular species, transcription of genes associated with lipid metabolism and oxidative status in broiler chickens fed flaxseed

Flaxseed is a rich source of α-linolenic acid (ALA, 18:3 n-3) and can be used to enrich chicken tissues with n-3 fatty acids (FA). However, antinutritional factors in flaxseed compromise the live performance of birds coupled with increased oxidative stress. Chromium (Cr) is a trace element with antioxidant properties. It is hypothesized that Cr supplementation will affect the hepatic total lipid profile, phospholipid n-3 and n-6 FA molecular species, lipid oxidation products, and transcription of genes associated with lipid metabolism in broiler chickens fed flaxseed. Ninety (n=90), day-old Cornish cross chicks were fed a corn-soybean meal-based diet containing 0% flaxseed (CTR), 10% flaxseed (FLAX), and FLAX+0.05% organic Cr (FLAXCr) for 42 d. The chicks were kept in 18 pens with 5 chicks per pen. For all response variables, the effect of dietary treatments were compared separately using SAS 9.4. P values were considered significant at ≤0.05. Total lipids, saturated FA, long-chain (≥20C) n-6 FA were reduced while total n-3 FA and long-chain n-3 FA were higher in the liver of FLAX and FLAXCr than CTR (P < 0.05). Hepatic phosphatidylcholine (PC) and phosphatidylethnolamine (PE) n-3 species (36:5, 38:6) were higher in FLAX and FLAXCr compared to CTR (P < 0.05). On the contrary, n-6 species in PC (36:4, 38:4) and PE (38:4) were lower in FLAX and FLAXCr compared to CTR (P < 0.05). Addition of Cr to a flaxseed-containing diet led to an increase in PE 36:4 (P < 0.05). A decrease in the transcription of ELOVL6 gene involved in de novo lipid synthesis was observed in FLAXCr (P=0.01). An increase in the transcription of genes involved in FA oxidation (ACAA2, ACOX1) was observed in FLAX compared to FLAXCr (P=0. 05; P=0.02). A trend for a decrease in the transcription of FADS2 and HMGCS1 was observed in FLAXCr than CTR and FLAX (P=0.06; 0.08). Transcription of other genes involved in de novo lipid synthesis (FASN, PPARA), FA oxidation (CPT1A, CPT2, ACAA1), and oxidative stress response (GPX1, NQO11, GSTA2, SLC40A1, NFE2L2) were not affected by the diets (P > 0.05). Lipid peroxidation products measured as thiobarbituric acid reactive substances (TBARS) in liver was reduced in FLAXCr than CTR (P < 0.05) and was not different from FLAX (P > 0.05). Serum cholesterol and aspartic aminotransferase were reduced in FLAX and FLAXCr compared to CTR (P < 0.05). The serum glucose level was decreased in FLAX compared to CTR (P < 0.05) and a trend in decrease was noticed in FLAXCr vs. CTR (P=0.10). Serum TBARS were higher in CTR and FLAXCr compared to FLAX (P < 0.05). In conclusion, flaxseed supplementation enhances total and long-chain n-3 FA while reducing total lipids, saturated, and n-6 FA in the liver. Supplementing Cr along with flaxseed increased n-6 FA species in the hepatic PE and decreased the transcription of genes involved in FA oxidation and lipid synthesis.

Effect of solid-state fermentation with Bacillus pumilus on the nutritional value, anti-nutritional factors and antioxidant activity of faba bean (Vicia faba L.) meal

To improve the utility value of faba bean meal (FBM), the effects of solid-state fermentation (SSF) by a strain of Bacillus pumilus on nutritional value, in vitro protein digestibility, anti-nutritional factors (ANFs), flavonoid, phenols and antioxidant activity were evaluated. The results revealed that the concentrations of ANFs (phytic acid and proanthocyanidin) were significantly decreased (P < 0.05) after fermentation. Fermented FBM showed higher nutritional value. Crude protein, amino acids and trichloroacetic acid-soluble protein contents in fermented FBM were higher than those from unfermented FBM. Meanwhile, the contents of phenols and flavonoid were remarkably (P < 0.05) increased after SSF. Scanning electron microscope showed that the surface of fermented FBM was more irregular and rougher than unfermented FBM. Moreover, SSF treatment significantly (P < 0.05) increased DPPH and superoxide anion radical scavenging capacities. The results indicated that SSF of FBM with B. pumilus is a promising and efficient method to improve nutritional value and antioxidant activity, as well as decrease the concentration of ANFs, which also endow the potential value of FBM on replacing soy bean as primary protein source in the feed ration of different animals.

Proximate composition and anti-nutritional factors in Mucuna pruriens (velvet bean) seed flour as affected by several processing methods

Mucuna pruriens seed being an underutilized legume with antinutrients was processed to reduce antinutrients and improve nutritional quality. The seeds were cleaned, washed and subjected to single treatments of soaking (24 h, 48 h, 72 h), cooking (20 min, 40 min, 60 min, 80 min), roasting (10 min, 15 min, 20 min), germination (24 h, 48 h, 72 h) and fermentation (24 h, 48 h, 72 h). Combined treatments: soaking (72 h) + cooking (60 min); germination (48 h) + roasting (15 min); germination (48 h) + cooking (60 min); fermentation (72 h) + roasting (15 min) were also separately carried out. Proximate composition and antinutrients were evaluated. Crude protein ranged from 25.34 to 29.50%, ash 3.02–3.82% and crude fibre 0.70–4.69. Crude protein was increased by single and double treatments while ash content was increased by only single treatments. All the treatments reduced the crude fibre. For single and double treatments, phenol, L-3,4-dihydroxyphenylalanine and trypsin inhibitor were in the range of 0.69–3.49%, 0.01–6.83% and 0.00–12.58 TIU/mg protein respectively. This research indicated that the use of up to 72 h soaking, 80 min cooking, 20 min roasting, 72 h germination or 72 h fermentation (Rhizopus oligosporus) is not adequate to reduce phenol and L-3,4-dihydroxyphenylalanine in Mucuna pruriens seed flour to below the recommended safe limits of 0.003% and 0.1% respectively. The double treatment of 72 h fermentation +15 m roasting was the only treatment that reduced L-3,4-dihydroxyphenylalanine to safe limit of 0.01%.

Phytoconstituents, GC-MS Characterization of Omega Fatty Acids, and Antioxidant Potential of Less-Known Plant Rivina humilis L.

Rivina humilis L. (Petiveriaceae), commonly known as the pigeon berry, accumulates betalains in berries. The present study was focused on identifying the phytoconstituents, mineral content, fatty acid composition, phenolics, flavonoids, antinutritional factors, and antioxidant activities of different plant parts (leaf, stem, root, and seeds), which are otherwise not well explored. Phytoconstituent analysis revealed seeds as a potential source of carbohydrates (50.15 g/100 g), proteins (10.96 g/100 g), and fats (11.25 g/100 g). Roots showed the highest fat (17.66 g/100 g) and dietary fiber (81.49 g/100 g). Leaves and roots contain more iron (29.59 and 29.39 mg/100 g), whereas seed has high zinc content (12.09 mg/100 g). Leaf oil showed 47.83 g/100 g of omega-3-fatty acid, confirmed by GC-MS analysis. Seed oil showed 22.23 g/100, 44.48 g/100, and 24.04 g/100 g of palmitic, oleic, and linoleic acids, respectively. The leaf extract has the highest TPC (597.55 mg/100 g), followed by the seed (421.68 mg/100 g). The leaf's 80% ethanolic extract had high TFC (2442.19 mg/100 g), followed by 70% methanolic extract (1566.25 mg/100 g). The antinutritional profile indicated significant phytic acid and oxalates in the leaf (9.3 g/100 and 2.07 g/100 g) and stem (6.9 and 1.58 g/100 g) and low tannin content (<0.5 g/100 g). The leaf's 80% ethanolic extract exhibited double the TAA than 70% methanolic extract (1.52 g/100 g). The leaf with an 80% ethanolic extract had the lowest DPPH and ABTS radical scavenging EC50 (2.22 and 0.37 mg/mL). The leaf with an 80% ethanolic extract (479.73 mg/100 g) and seed (391.14 mg/100 g) had the highest FRAP activity. Our study proves that different parts of R. humilis had a good content of phytoconstituents, bioactives, and antioxidant activities. Hence, R. humilis leaves and seeds are a novel source of omega fatty acids and minerals reported for the first time and have potential applications in the food and pharmaceutical industries.

Effects of pre-treatments on nutritional and mineral composition of garden cress seeds (Lepidium sativum)

This study aimed to recognize an effective treatment-time combination for improving nutritional value of Garden cress seeds (GCS). In this study GCS were subjected to different pre-treatments combinations viz., BoR- boiling (10 min) + roasting (2.5, 5 and 7.5 min), BlR- blanching (3 min) + roasting (2.5, 5 and 7.5 min) and SR- soaking (12 hrs) + roasting (2.5, 5 and 7.5 min), respectively. Results revealed for proximate composition showed slight variation in protein content ranged from 21.08 to 22.90% among treatments. Results for treatment SR 2.5 showed significant (p<0.05) increase in ash content from 4.62 to 5.24%. Results reported for treatment BoR showed significant improvement in iron content (10.84 mg/100 g) with increase in roasting time i.e., treatment BoR 7.5. The significant (p < 0.05) decrease in anti-nutritional factors from 458.3 to 414.5 mg/100 g for phytates, and 152.11 to 93.93 mg/100 g for oxalates were observed in treatment SR 7.5 with maximum retention of calcium of 378 mg/100 g. It could be concluded that overall improvement in nutrient content and maximum nutrient retention was observed during soaking and subsequent roasting. Thus, treatment SR 7.5 can be considered as best pre-treatment for enhanced nutritional value and decreased antinutritional profile of GCS.

Effects of Processing Methods on Anti-Nutrients and Proximate Compositions of Aizien B. Senegalensis Seed as Alternative Protein Source for Fish Feed

The study investigated the nutritional suitability Boscia senegalensis (Aizen) as alternative for protein in the formulation of diet of fish diet. The seeds were subjected to different processing methods to reduce levels of the most important antinutritional factors (ANFs) in the seeds. Standard methods of analysis were used for determination of anti-nutrients and proximate compositions of the processed seeds. Analyzed anti-nutritional factors of the differently processed B. senegalensis seeds indicated that soaking was the best in reducing the anti-nutrients with lowest levels of Saponin (2.10 m/g), Phytic acid (0.10 mg/g), Flavonoids (72.8 mg/g), Tanin (0.6 mg/g) and Alkaloids (1.02 mg/g). Proximate analysis showed that crude protein (CP) composition of the differently processed Boscia senegalensis seed ranged from 28.48 – 31.50%. Soaked sample had the highest CP (31.50%) while the boiled sample was the lowest (28.48%). Decreased lipid and fibre contents were observed in the boiled, fermented and the soaked seeds respectively. The study recommends soaking as best treatment for B. senegalensis seeds while proximate analysis revealed that treated B. senegalensis seed could be good alternative source of protein for fish feed formulation.

Faba Bean Processing: Thermal and Non-Thermal Processing on Chemical, Antinutritional Factors, and Pharmacological Properties.

The food industry, academia, food technologists, and consumers have become more interested in using faba bean seeds in the formulation of new products because of their nutritional content, accessibility, low costs, environmental advantages, and beneficial impacts on health. In this review, a systematic and up-to-date report on faba bean seeds' antinutrients and bioactive and processing techniques is comprehensively presented. The chemical composition, including the oil composition and carbohydrate constituents, is discussed. Factors influencing the reduction of antinutrients and improvement of bioactive compounds, including processing techniques, are discussed. Thermal treatments (cooking, autoclaving, extrusion, microwaving, high-pressure processing, irradiation) and non-thermal treatments (soaking, germination, extraction, fermentation, and enzymatic treatment) are identified as methods to reduce the levels of antinutrients in faba bean seeds. Appropriate processing methods can reduce the antinutritional factors and enrich the bioactive components, which is useful for the seeds' efficient utilization in developing functional foods. As a result, this evaluation focuses on the technologies that are employed to reduce the amounts of toxins in faba bean seeds. Additionally, a comparison of these methods is performed in terms of their advantages, disadvantages, viability, pharmacological activity, and potential for improvement using emerging technologies. Future research is expected in this area to fill the knowledge gap in exploiting the nutritional and health benefits of faba bean seeds and increase the utilization of faba bean seeds for different applications.

Strategies for identifying stable lentil cultivars (Lens culinaris Medik) for combating hidden hunger, malnourishment, and climate variability.

Iron and zinc malnutrition is a global humanitarian concern that mostly affects newborns, children, and women in low- and middle-income countries where plant-based diets are regularly consumed. This kind of malnutrition has the potential to result in a number of immediate and long-term implications, including stunted growth, an elevated risk of infectious diseases, and poor development, all of which may ultimately cause children to not develop to the fullest extent possible. A determination of the contributions from genotype, environment, and genotype by environment interactions is necessary for the production of nutrient-dense lentil varieties that offer greater availability of iron and zinc with a high level of trait stability. Understanding the genotype and environmental parameters that affect G x E (Genotype x Environment) interactions is essential for plant breeding. We used GGE(Genotype, Genotype x Environment interactions) and AMMI (Additive Main effects and Multiplicative Interaction) models to study genetic stability and GE(Genotype x Environment interactions) for grain Fe, Zn, Al, and anti-nutritional factors like phytic acid content in sixteen commercially produced lentil cultivars over several different six geographical locations across India. Significant genetic variability was evident in the Fe and Zn levels of different genotypes of lentils. The amounts of grain iron, zinc, and phytic acid varied from 114.10 to 49.90 mg/kg, 74.62 to 21.90 mg/kg, and 0.76 to 2.84 g/100g (dw) respectively. The environment and GE (Genotype x Environment interactions) had an impact on the concentration of grain Fe, Zn, and phytic acid (PA). Heritability estimations ranged from low to high (53.18% to 99.48%). The study indicated strong correlation between the contents of Fe and Zn, a strategy for simultaneously increasing Fe and Zn in lentils may be recommended. In addition, our research revealed that the stable and ideal lentil varieties L4076 (Pusa Shivalik) for Fe concentration and L4717 (Pusa Ageti) for Zn content, which have lower phytic acid contents, will not only play an essential role as stable donors in the lentil bio-fortification but will also enable the expansion of the growing area of bio-fortified crops for the security of health and nutrition.

A chestnut-hemp type-II sourdough to improve technological, nutritional, and sensory properties of gluten-free bread

The nutritional quality of gluten-free (GF) products is usually improved by using flours derived from alternative grains (e.g., pseudocereals and legumes), additives and hydrolysates, leading to long ingredient lists in the labels, that conflict with current customer expectations. In this work, chestnut, carob, and hemp flours were used as mixed ingredients for making a gluten-free type-II sourdough. Three exopolysaccharides-producer lactic acid bacteria, belonging to Leuconostoc mesenteroides, Weissella cibaria, and Leuconostoc pseudomesenteroides, were used, and the fermentation processes (6 log10 cfu/g, 25 °C, 16 h) optimize to maximize the EPS synthesis (15.70 ± 2.1 mg/kg). The chestnut-hemp (70:30) type-II sourdough was included in a rice/corn gluten-free bread recipe also containing psyllium flour as structuring agent. Although the fortification with unfermented flours already led the achievement of 6 g/100 g of fiber (high fiber, Regulation EC n. 1924/2006) and content of magnesium higher than the daily reference intakes, the use of type-II sourdoughs led to a further structural, sensory, and nutritional improvements (e.g., decreasing the main anti-nutritional factor phytic acid). This work demonstrated that the use of ad-hoc selected ingredients and optimized protocol can be used to produce a GF and “clean label” bread with optimal nutritional features and appreciable sensory and structural properties.

Characteristics of Whole Wheat, Red Kidney Bean and Defatted Coconut Flour Blends and Its Application in Bread Production

Aim: To evaluate the functional, proximate and anti-nutrient content of flour blends and sensory properties of bread produced from blends. Methodology: Flour was prepared from whole wheat (WW), red kidney bean (RKB) and coconut fruit (CF). It was than blend as follows: S1 (100% refined wheat), S2 (100% WW), S3 (90% WW, 5% RKB and 5% CF), S4 (85% WW, 10% RKB and 5% CF), S5 (80% WW, 15% RKB and 5% CF), S6 (75% WW, 20% RKB and 5% CF). Blends were subjected to functional, proximate and anti nutrient content analyses. Also the sensory attributes of breads produced from blends were determined. Results: Water absorption, oil absorption, swelling, foaming, foaming stability and emulsification capacity increased respectively from 0.62±0.06g/ml (S1) to 0.92±0.01 g/ml (S6), 0.96±0.02g/ml (S1) to 1.42±0.03 g/ml (S6), 1.05±0.01 (S1) to 1.73±0.01 (S6), 11.47±0.02% (S1) to 13.20±0.05% (S6), 50.93±0.01% (S1) to 53.47±0.01% (S6), 41.43±0.03% (S1) to 43.36±0.01% (S6) while bulk density decreased from 0.84±0.01g/ml (S1) to 0.60±0.02g/ml (S6). Moisture, ash, fat, fiber, and protein content increased respectively from 12.23±0.03% (S1) to 13.44±0.01% (S6), 0.66±0.01% (S1) to 1.66±0.02% (S6), 0.22±0.3% (S1) to 2.57±0.01% (S6), 0.67±0.26% (S1) to 4.34±0.02% (S6), 10.70±0.12% (S1) to 13.06±0.03% (S6) while carbohydrate decreased from 75.52±0.33% (S1) to 64.93±0.04% (S6). Phytate, oxalate and tannins content increased respectively from 0.03±0.00mg/100g (S1) to 1.09±0.01mg/100g (S6), 0.02±0.02mg/100g (S1) to 0.14±0.01mg/100g (S6), 0.01±0.01mg/100g (S1) to 0.09±0.01mg/100g (S6). In term of overall acceptability sample S1 and S3 showed no significantly different (p &gt; 0.05). Conclusion: Addition of red kidney beans and coconut flour into whole wheat had significant (P&lt;0.05) impact on the functional, proximate characteristic of flour. There sensory scores showed S3 to be suitable for bread.

Replacement of dietary fish meal by fermented soybean meal improves growth, energy metabolism, and innate immune response in Penaeus vannamei Boone, 1931 (Decapoda: Dendrobranchiata: Penaeidae)

Abstract Fishmeal (FM) is considered a preferred source of protein for aquatic feed due to its good palatability and digestibility, a balanced amino acid profile, and a low level of anti-nutritional factors (ANFs). Shortage of FM supply and increases in price, however, are major challenges faced by feed producers and aquaculture farmers. Fermented soybean meal (FSBM) has been proposed to partially replace FM because of its high nutritional value and low cost. The optimal FSBM concentration for substituting FM and effects of FSBM substitution on growth performance, energy metabolism, and immune response nevertheless remain unclear. We evaluated the effects of shrimp diets containing FSBM at 0% (FM), 25% (FSBM25), 50% (FSBM50), 75% (FSBM75), and 100% (FSBM100) on the shrimp Penaeus vannameiBoone, 1931. FSBM50 led to a significantly higher weight gain (postlarvae 1927.36 ± 46.64%, young juveniles 237.00 ± 8.25%, and juveniles 153.96 ± 2.18%) than the other experimental and control diets in different stages of the shrimp. The FM group consumed less oxygen than the experimental diet groups. FSBM75 and FSBM100 led to a significantly higher ammonia-N excretion than FM, FSBM25, and FBSM50. All experimental diet groups exhibited a significantly higher O:N ratio (8.3 ± 0.8–9.4 ± 1.7) than the FM group (7.1 ± 0.2). Shrimp fed with FSBM25- and FSBM50-containing diets exhibited an increased total hemocyte count, and FSBM25, FBSM50, and FSBM100 led to a significantly higher superoxide anion production than FSBM75 and FM. FSBM substitution in shrimp diets resulted in a significantly higher phagocytic activity than the FM diet, suggesting that FSBM substitution improves the cell-mediated immune response in shrimps. In challenge trials with white spot syndrome virus (WSSV), all experimental diets led to a higher survival rate than the FM diet, and in Vibrio parahaemolyticus challenge trials, FSBM50 led to a higher survival rate than the other experimental and control diets, indicating that FSBM substitution in shrimp diets could improve the defense capacity of shrimps against WSSV and V. parahaemolyticus.

Nutritional Composition and Antinutritional Factor Analysis of Kpokpogari and Starch: A Comparative Study Using Commercial Wheat

This study aimed to conduct a comparative analysis of the nutritional composition and antinutritional factors in kpokpogari, starch, and a commercial wheat product. The study employed proximate analysis to determine the macronutrient composition, mineral analysis to assess the presence of essential minerals, and vitamin analysis to evaluate the vitamin content. Additionally, antinutritional factors such as phytates, tannins, and oxalates were analyzed to identify any potential limitations or health concerns associated with these foods. The findings revealed that kpokpogari and starch were primarily carbohydrate-rich foods, while the commercial wheat product had a higher protein content. When compared to the typical diet of the elderly, the control has much higher levels of vital minerals. The salt content in the control group was the lowest (0.11 mg/100 g). Foods (kpokpogari and starch) typically consumed by the elderly had lower vitamin content than the control. Kpokpogari and starch had the highest levels of all the antinutritional components measured, ranging from 1.15 mg/100 g glycoside to 0.02 mg/100 g phytate. The study provides valuable insights into the nutritional composition of traditional Nigerian foods and their potential health implications. Future research can further explore their nutritional value and promote healthier dietary choices.

Characterization of bioprocessed white and red sorghum flours: Anti-nutritional and bioactive compounds, functional properties, molecular, and morphological features.

In the current investigation, white and red sorghum grains were exposed to bioprocessing techniques, such as soaking, germination, fermentation, and dual processing (both germination and fermentation). Germination and fermentation resulted in improved bioactive profile attributing to better antioxidant activity, whereas a reduction in antinutrient components was observed. On the other hand, soaking had decreased phenolic components and anti-nutritional factors attributing to their leaching in the soaking water. A significant change in the functional properties and color profile was also observed on bioprocessing. It also caused alterations in the morphological structure of the starch-protein matrix and molecular interactions of certain functional groups that reveal the synthesis of certain new bioactive compounds in the flour. The alterations in the bioprocessed flours occurred due to the structural breakdown attributing to the activity of hydrolytic enzymes that were activated during the processing treatments. Bioprocessing was also responsible for the degradation of the starch granules and unfolding of the protein matrix, thus altering the in vitro nutrient digestibility of the flours. Principal component analysis was used to authenticate the differences between different treatments and observations recorded. These bioprocessed flours could be potential ingredients for several valorized cereal products.

Effect of Sand Roasting on Physicochemical, Thermal, Functional, Antinutritional, and Sensory Properties of Sattu, a Nourishing form of Chickpea

Sattu is a traditional Indian food product made of chickpea with tremendous nutritional benefits. However, the processing of sattu has not been thoroughly explored which is an impediment to industrial applications involving the development of sattu-based products. These products carry immense benefits for consumers and for widespread popularity; it is essential that the roasting of sattu for further processing be investigated for improving the properties of sattu while reducing its antinutritional factors. In this study, the impact of sand roasting on the physicochemical, sensory, antinutritional, thermal, functional, and antioxidant properties on sattu was investigated. Chickpea grains were roasted in sand for different time periods (3–17 min) and temperature combinations (171–228°C). Results revealed that samples treated at 180°C for 15 min had maximum sensory score (3.99) followed by the samples treated at 200°C for 10 min and at 228°C for 10 min. Overall sattu roasted at 180°C for 15 min was found suitable for further application.

Overview of the Incorporation of Legumes into New Food Options: An Approach on Versatility, Nutritional, Technological, and Sensory Quality.

Consumers are more aware and demanding of healthy food options, besides being concerned with environment-friendly consumption. This paper aims to evaluate nutritional, technological, and sensory characteristics of legumes and their products' quality and versatility, considering potential applications in new food options. Legumes are foods that have a recognized nutritional group since they have high protein and fiber content. However, their consumption is still somehow limited for some reasons: in some countries it is not easy to find all the species or cultivars, they need an organization and planning before preparation since they need soaking, and there is the presence of antinutritional factors. Due to the different functionalities of legume proteins, they can be applied to a variety of foods and for different purposes, as grains themselves, aquafaba, extracts, flours, brans, and textured proteins and sprouts. These products have been inserted as ingredients in infant food formulations, gluten-free foods, vegetarian diets, and in hybrid products to reduce food costs as well. Foods such as bread, cakes, cookies, meat analogues, and other baked or cooked products have been elaborated with nutritional, technological and sensory quality. Further development of formulations focused on improving the quality of legume-based products is necessary because of their potential and protein quality.

Comparative effects of soy protein concentrate, enzyme-treated soybean meal, and fermented soybean meal replacing animal protein supplements in feeds on growth performance and intestinal health of nursery pigs

BackgroundSoy protein supplements, with high crude protein and less antinutritional factors, are produced from soybean meal by different processes. This study evaluated the comparative effects of various soy protein supplements replacing animal protein supplements in feeds on the intestinal immune status, intestinal oxidative stress, mucosa-associated microbiota, and growth performance of nursery pigs.MethodsSixty nursery pigs (6.6 ± 0.5 kg BW) were allotted to five treatments in a randomized complete block design with initial BW and sex as blocks. Pigs were fed for 39 d in 3 phases (P1, P2, and P3). Treatments were: Control (CON), basal diet with fish meal 4%, 2%, and 1%, poultry meal 10%, 8%, and 4%, and blood plasma 4%, 2%, and 1% for P1, P2, and P3, respectively; basal diet with soy protein concentrate (SPC), enzyme-treated soybean meal (ESB), fermented soybean meal with Lactobacillus (FSBL), and fermented soybean meal with Bacillus (FSBB), replacing 1/3, 2/3, and 3/3 of animal protein supplements for P1, P2, and P3, respectively. Data were analyzed using the MIXED procedure in SAS 9.4.ResultsThe SPC did not affect the BW, ADG, and G:F, whereas it tended to reduce (P = 0.094) the ADFI and tended to increase (P = 0.091) crypt cell proliferation. The ESM did not affect BW, ADG, ADFI, and G:F, whereas tended to decrease (P = 0.098) protein carbonyl in jejunal mucosa. The FSBL decreased (P < 0.05) BW and ADG, increased (P < 0.05) TNF-α, and Klebsiella and tended to increase MDA (P = 0.065) and IgG (P = 0.089) in jejunal mucosa. The FSBB tended to increase (P = 0.073) TNF-α, increased (P < 0.05) Clostridium and decreased (P < 0.05) Achromobacter and alpha diversity of microbiota in jejunal mucosa.ConclusionsSoy protein concentrate, enzyme-treated soybean meal, and fermented soybean meal with Bacillus could reduce the use of animal protein supplements up to 33% until 7 kg body weight, up to 67% from 7 to 11 kg body weight, and entirely from 11 kg body weight without affecting the intestinal health and the growth performance of nursery pigs. Fermented soybean meal with Lactobacillus, however, increased the immune reaction and oxidative stress in the intestine consequently reducing the growth performance.

Effect of physical treatments on phenolics and tannins in Ficus roxburghii and Quercus leucotrichophora leaves

Inadequate availability of straws and conventional concentrates is one of the major constraints for rearing large and small ruminants. Alternative or unconventional fodder resources play a supplementary role in meeting the demand of livestock owners; but these tree leaves contain some antinutritional factors. Tremal (Ficus roxburghii) and Oak (Quercus leucotrichophora) are among the traditional tree fodder sources available in the North Western Himalayan region. Their use a feed stuff for livestock is limited due to presence of tannis. So, the study was undertaken to assess the effect of different physical treatments on total phenol and tannin fractions of Tremal and Oak leaves. Fresh leaves lopped from Tremal and Oak trees of Kangra district of Himachal Pradesh were subjected to physical treatments like chopping and sun drying after chopping. The phenol and tannin fractions of these leaves were estimated before and after treatment. Total phenols, total tannin and hydrolysable tannin contents of the F. roxburghii and Q. leucotrichophora leaves were reduced significantly (P&lt;0.0001) by both the physical treatments. However, both the treatments increased (P ≤ 0.0001) the condensed tannin content of F. roxburghii leaves, whereas chopping followed by sun drying also increased the non-tannin phenol contents. Both the treatments were effective in reducing tannin contents (both hydrolysable and condensed) of Q. leucotrichophora leaves. It may be concluded that physical treatments, chopping, chopping and sun drying, were though effective in reducing hydrolysable tannins, but were not effective in reducing condensed tannin contents of F. roxburghii leaves; whereas chopping, chopping and sun drying were effective in reducing tannin contents in case of Q. leucotrichophora leaves.

How superheated steam treatment modifies the physicochemical, functional, nutritional, and anti-nutritional attributes: A case study on guar bean flour

Guar seed flour (GSF) is a nutritionally rich ingredient that is widely available, but antinutritional factors limit its commercial use. Considering this limitation, the present study investigated the effect of superheated steam (SHS) treatment (120–150 °C, 5–15 min) on the nutritional properties, bioactive compounds, anti-nutritional factors, thermo-pasting and structural characteristics, and in-vitro digestibility of GSF. The SHS treatment influenced the carbohydrate, protein, and fat content and improved the functional properties of GSF. Similarly, an increment in total phenolic content, antioxidant activity, and a decline in total flavonoid content was observed. The maximum reduction of tannin, phytic acid, and saponin was observed in GSF samples treated at 150 °C – 15 min. The diffractogram pattern showed a decrease in crystallinity with increasing SHS temperature and duration, trypsin inhibitor activity was reduced, while the in-vitro digestibility increased. Apart from this, the SHS treatment also influenced the thermo-pasting properties of GSF, which might be due to the intermolecular interactions, cross-linking, and starch-lipid interactions. Industrial relevanceThe SHS treatment provides high energy efficiency and has the potential to be commercialized on an industrial scale in the food industry, despite its popularity in the non-food sector. The findings indicate that SHS treatment under optimal conditions improved GSF's physicochemical, functional, and digestibility properties. Furthermore, the SHS treatment was very effective at reducing anti-nutritional factors. The product developed from SHS-treated GSF has the potential to be a viable alternative for food formulations and will have a positive impact on human health.

A Comprehensive Review of Pea (Pisum sativum L.): Chemical Composition, Processing, Health Benefits, and Food Applications.

Pisum sativum L., commonly referred to as dry, green, or field pea, is one of the most common legumes that is popular and economically important. Due to its richness in a variety of nutritional and bioactive ingredients, the consumption of pea has been suggested to be associated with a wide range of health benefits, and there has been increasing focus on its potential as a functional food. However, there have been limited literature reviews concerning the bioactive compounds, health-promoting effects, and potential applications of pea up to now. This review, therefore, summarizes the literature from the last ten years regarding the chemical composition, physicochemical properties, processing, health benefits, and potential applications of pea. Whole peas are rich in macronutrients, including proteins, starches, dietary fiber, and non-starch polysaccharides. In addition, polyphenols, especially flavonoids and phenolic acids, are important bioactive ingredients that are mainly distributed in the pea coats. Anti-nutritional factors, such as phytic acid, lectin, and trypsin inhibitors, may hinder nutrient absorption. Whole pea seeds can be processed by different techniques such as drying, milling, soaking, and cooking to improve their functional properties. In addition, physicochemical and functional properties of pea starches and pea proteins can be improved by chemical, physical, enzymatic, and combined modification methods. Owing to the multiple bioactive ingredients in peas, the pea and its products exhibit various health benefits, such as antioxidant, anti-inflammatory, antimicrobial, anti-renal fibrosis, and regulation of metabolic syndrome effects. Peas have been processed into various products such as pea beverages, germinated pea products, pea flour-incorporated products, pea-based meat alternatives, and encapsulation and packing materials. Furthermore, recommendations are also provided on how to better utilize peas to promote their development as a sustainable and functional grain. Pea and its components can be further developed into more valuable and nutritious products.