Sponge samples coded KL-03P, KL-12P, KL-15P, KL-16P, and KL-18NP collected from Lugait, Misamis Oriental, Philippines were subjected to fractionation resulting in hexane (H), dichloromethane (D), and aqueous (A) fractions of each sponge. They were subsequently subjected to anti-migration, anti-proliferative, and pro-apoptotic activity testing using the Scratch Wound assay, the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay, and the Caspase Glo 3/7 assay, respectively. Fractions KL-03P (D), KL-12P (D), KL-16P H) and KL18NP (D) showed anti-proliferative and pro-apoptotic effects against HCT116 at 30-ppm concentration. Cell shrinkage and noticeable fragmentations indicative of apoptosis were apparent among these fractions based on MTT final snaps. But among these active fractions, the unprecedented abilities of KL-18NP (D) were very notable across assays conducted. This fraction showcased the inhibition of cell migration, which gave a signal for potentially reduced oncogenicity or metastatic capability of HCT116. The negative percentage of wound healing exhibited by this fraction is a manifestation of its cytotoxicity effect. Very remarkably, KL-18NP (D) has a similar degree of responses across the positive controls, respectively. Hence, there is a significant negative relationship between the negative control versus KL-18NP (D) in terms of its anti-migratory and anti-proliferative properties, p<0.05. Additionally, there is a significant positive relationship between the negative control and KL-18NP (D) when it comes to its pro-apoptotic capability, p<0.05. These results make KL-18NP (D) a bioactive hit as an anti-cancer agent source. Confirmatory techniques, structure determination, and target identification methods will further ascertain its mechanism of action as well as the identification of its cellular targets.