Anti-kidney Antibodies Research Articles

The Zone of Localization of Antibodies

Summary An attempt has been made to saturate the anti-kidney antibody-binding sites in the mouse kidney. The administration of even 15 mg of a globulin fraction of anti-mouse-kidney rabbit serum (G-anti-MK) did not saturate these sites, since this quantity of unlabeled anti-kidney antibody had no appreciable effect on the renal accumulation of radioactivity in animals that simultaneously were given radioiodinated G-anti-MK. A minimum value for the amount of antibody which can localize in the kidney was calculated, and was found to correspond to about 3 per cent of the weight of a mouse's glomeruli. When large amounts (about 15 mg) of either G-anti-MK or globulin fraction of anti-mouse-spleen rabbit serum (G-anti-MS) were given along with radioiodinated G-anti-MK there occurred an unusually rapid decline in the blood levels of radioactivity, indicating a physiological effect of these sera.

The Zone of Localization of Antibodies

Summary The rate at which antibodies to mouse kidney are extracted from the circulating blood was measured by using antibody tagged with radioactive iodine. It was found that the circulating blood was essentially freed of antibody within 18 minutes after intravenous injection. Some localization of the radioactive anti-kidney antibody in the glomeruli was observable within as little as 2.5 minutes after injection.