Anticonvulsant Activity Of Ethanolic Extract Research Articles

Evaluation of Anticonvulsant Activity of Ethanolic Extract of Leaves of Cajanus cajan (L) Millsp. In Rodents

Cajanus cajan (L) Millsp. is the perennial plant belongs to family Fabaceae, commonly called as Pigeon pea plant. The presence of phytoconstituents like flavonoids, the flavanone (substituted) from Cajanus cajan (L) Millsp. have in vitro neuroactive property. This flavanone named as pinostrobin helps to inhibit voltage – gated sodium channels. Because of its bioactive phytoconstituents it may act as antiepileptic drug. To avoid problems like ADR herbal plant might be alternative to treat epilepsy. The current study was therefore carried out to evaluate antiepileptic activity of Ethanolic extract of leaves of Cajanus cajan in rodents. The effect of ELECC in MES-induced convulsions in rat and PTZ-induced convulsion in mice was evaluated using doses 100 mg/kg and 200 mg/kg for 7 days. Phenytoin (25 mg/kg), Diazepam (4 mg/kg) was used as standard drug for respective model. Depending on the model, outcome measures were abolishment of Hind Limb Tonic Extensor phase in MES-induced convulsion in rat and onset of latency, recovery or death in PTZ-induced convulsion in mice as well as biochemical estimation of amino acid neurotransmitter (GABA, Glutamate) were evaluated. The ELECC at doses 100 and 200 mg/kg significantly delayed the HLTE phase in MES-induced convulsions in rat whereas, significantly increased onset of latency in PTZ-induced convulsion in mice. It also showed significant (p>0.0001) effect on the level of GABA and Glutamate in dose dependent manner in both models. The phytochemical study of C. cajan showed the presence of Glycosides, Flavonoids, Flavonones, Steroids, Tannins, Fixed oil, Fatty acids and Proteins. As the flavonoids present in C. cajan may contribute to the anticonvulsant activity of the plant. Therefore, the presence of such compounds in the extract may be responsible for the anticonvulsant effect. Therefore, present study validates its anticonvulsant activity. Further, research is required to elucidate its specific mechanism of action and isolation of responsible active principles.

STUDY OF THE ANTICONVULSANT ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF ACORUS CALAMUS IN ALBINO RATS

Objective: Root of Acorus calamus has been traditionally used as an anticonvulsant. The aim of the study is to assess the anticonvulsant activity of ethanolic extract of A. calamus (EEAC) by maximal electroshock seizure (MES) and pentylenetetrazol (PTZ)-induced seizure models on albino (Wistar strain) rats.Methods: Albino rats were taken and divided into five groups, each consisting of five rats both for MES and PTZ model. One group was used as control (normal saline 10 ml/kg), one as standard (phenytoin in MES model/diazepam in PTZ model), and three groups for the test drug (EEAC in the doses of 100, 200, and 400 mg/kg). In MES model, maximal electrical shock of 150 mA was passed for 0.2 s through earlobe electrodes after 30 min of giving the drugs and normal saline. Different stages of convulsions were noted down along with time spent by the animal in each phase of convulsions. In PTZ model, PTZ was injected 30 min after giving the drugs and normal saline, and onset of action and severity of convulsions were noted. Data were statistically analyzed by one-way analysis of variance followed by multiple Dunnett’s test.Results: EEAC dose dependently reduced the duration of tonic hind limb extension in MES model, and there was increase in latency and occurrence of convulsions in PTZ model.Conclusion: EEAC has anticonvulsant activity.

STUDY OF THE ANTICONVULSANT ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF ACORUS CALAMUS IN ALBINO RATS

Objective: Root of Acorus calamus has been traditionally used as an anticonvulsant. The aim of the study is to assess the anticonvulsant activity of ethanolic extract of A. calamus (EEAC) by maximal electroshock seizure (MES) and pentylenetetrazol (PTZ)-induced seizure models on albino (Wistar strain) rats.Methods: Albino rats were taken and divided into five groups, each consisting of five rats both for MES and PTZ model. One group was used as control (normal saline 10 ml/kg), one as standard (phenytoin in MES model/diazepam in PTZ model), and three groups for the test drug (EEAC in the doses of 100, 200, and 400 mg/kg). In MES model, maximal electrical shock of 150 mA was passed for 0.2 s through earlobe electrodes after 30 min of giving the drugs and normal saline. Different stages of convulsions were noted down along with time spent by the animal in each phase of convulsions. In PTZ model, PTZ was injected 30 min after giving the drugs and normal saline, and onset of action and severity of convulsions were noted. Data were statistically analyzed by one-way analysis of variance followed by multiple Dunnett’s test.Results: EEAC dose dependently reduced the duration of tonic hind limb extension in MES model, and there was increase in latency and occurrence of convulsions in PTZ model.Conclusion: EEAC has anticonvulsant activity.

Evaluation of anticonvulsant activity of the ethanolic extracts from leaves of Excoecaria agallocha

Ethnopharmacological relevanceExcoecaria agallocha is popularly known as ‘Thillai’ plant in the tamil language of the South India. Its uses are mentioned on the temple inscription of the Chidambaram Temple, Chidambaram, India which dates back to 2nd Century CE. AimTo investigate the anticonvulsant activity of ethanolic extract of leaves mangrove plant Excoecaria agallocha. Material and methodsThe ethanolic leaf extract of Excoecaria agallocha (100, 200 and 40 mg/kg) was evaluated in Swiss albino mice and male wistar rats were used for the study. Two different study models were employed; Maximal electroshock and Lithium-Pilocarpine convulsion method. The ethanolic extract was also subjected to acute toxicity study and phytochemical evaluation. ResultsThe ethanolic extract of leaves of Excoecaria agallocha was found to be safe in toxicity studies when compared to the standard drugs. It has LD50 of 2.12 g/kg of oral dose and 3.12 mg/kg of intraperitoneal dose. This extract (100–400 mg/kg) produced a significant (P < 0.01) dose dependent delay in onset of seizures in the MES model on oral administration which is comparable to Phenytoin (20 mg/kg i. p). This extract (100–400 mg/kg) produced a significant (P < 0.01) dose dependent reduction in intensity of seizures in the Lithium-Pilocarpine epilepsy model which was comparable to the standard drug diazepam (10 mg/kg). ConclusionThe results obtained from this study and finding suggests for the use of Excoecaria agallocha for the management of convulsions and justifies its use in traditional medicine.

Evaluation of anticonvulsant activity of ethanolic extract of Gomphrena serrata by using Swiss albino mice

Epilepsy is characterised by abnormal behaviour which is leading to tonic flexon, tonic extension, clonus and stupor. Many novel therapeutic regimens were used to treat these disorders through different ways including altering neurotransmission, but so far there is no specific treatment approach which is satisfactory to the patients in terms of complete cure. Our approach is to make understand the herbal medicines usage towards epilepsy. The ethanolic plant extract of Gomphrena Serrata at 400mg/kg, 600mg/kg and 800mg/kg were given to albino mice which were treated with maximum electric shock of 30mA current and pentelene tetrazolium in two different techniques. The results with these extract doses showed significant results which indicated decrease in clonic extension and stupor. Whereas there is no decrease in the tonic flexon observed with all doses. All these results were compared with the standard drug Phenytoin at 25mg/kg I.P. However, the ethanolic plant extract of Gomphrena Serrata at 600mg/kg showed marked increase in the therapeutic activity which is equivalent to Phenytoin and can be compared. Apart from these the ethanolic plant extract of Gomphrena Serrata at 400mg/kg, 600mg/kg and 800mg/kg showed significant decrease in the recovery times when compared to control group.

To evaluate the anticonvulsant activity of ethanolic extract of Moringa oleifera (drumstick leaves) in albino mice

Background: To evaluate the anti-convulsant activity of ethanolic extract of Moringa oleifera (Drum stick leaves) in seizure induced albino mice and to compare it with standard drug Sodium valproate.Methods: Swiss albino mice of either sex weighing around 25-30g were randomly selected and divided into four groups of six mice each. Group 1: control- treated with gum acacia. Group 2: Standard - Valproic acid 40mg/kg body weight. Group 3: T1- ethanolic extract of Moringa oleifera (150mg/kg). Group 4: T2 - ethanolic extract of Moringa oleifera (300mg/kg). All drugs were administered orally one hour prior to induction of seizure. The anticonvulsant activity was screened using maximal electroshock seizure (MES) model and pentylenetetrazole (PTZ) model.Results: Results were analysed by ANOVA followed by Bonferroni’s post hoc test. Abolition of Tonic hind limb extension was taken as the protective end point against MES induced seizures and prolongation of seizure latency in PTZ model.At both the doses the ethanolic extract of Moringa oleifera significantly (p value &lt;0.05) reduced the duration of hind limb extension in MES test and also significantly (p value &lt;0.05) delayed the onset of clonic seizures in PTZ induced convulsion when compared with control group.Conclusions: On comparing the percentage protection offered by Moringa oleifera leaves against both MES and PTZ model, it possesses significant anticonvulsant activity at both doses, with more efficacy at 300mg/kg BW indicating that the test drug can prove a very promising drug for treatment of epilepsy. Further studies are required for isolation and identification of the active constituent.

Evaluation of anticonvulsant activity of ethanolic extract of leaves of Ocimum sanctum (tulsi) in albino rats

Background: Epilepsy is a chronic neurological disorder. Ocimum sanctum (OS) (tulsi) has analgesic, anticancer, antiasthmatic, antidiabetic, antifertility, hepatoprotective, hypotensive, hypolipidemic, anti-inflammatory, antioxidant, immune modulatory, and antistress properties. Aims and Objectives: The objective was to evaluate the anticonvulsant effect of ethanolic extract of leaves of OS and to compare the anticonvulsant effect with the standard sodium valproate in electrically and chemically induced epileptic animal models. Materials and Methods: A total of 60 albino rats (150-200 g) of male sex were randomly selected. They were divided into five groups (per model) of six rats each. Control group received normal saline (0.35 ml/100 g), standard group sodium valproate (300 mg/kg), and the test groups were given OS at three different doses (1.75, 4.25, and 8.5 mg/kg). The anticonvulsant activity was screened using maximal electroshock seizure (MES) model and pentylenetetrazole (PTZ) model. Results were analyzed by ANOVA followed by post-hoc turkey’s test. Results: The ethanolic extract of OS leaves at doses of 4.25 and 8.5 mg/kg has shown significant anticonvulsant by decreasing the duration of tonic hind limb extension activity in MES model and by prolonging the duration of seizure latency in PTZ model. Conclusion: The ethanolic extract of OS leaves possess anticonvulsant activity which can be comparable with the standard sodium valproate.

ANTICONVULSANT ACTIVITY OF CITRUS MAXIMUS LEAVES IN EXPERIMENTAL ANIMAL MODELS

ABSTRACTObjective: To assess the anticonvulsant activity of ethanolic extract of Citrus maximus (EECM) leaves of maximal electroshock seizure (MES) andpentylenetetrazol (PTZ)-induced seizure models on albino (Wistar strain) rats and mice.Methods: Anticonvulsant activity was carried out by MES model and PTZ-induced clonic convulsions model; in each model, albino rats (Wistar strain)of either sex were taken and divided into five groups, each consisting of 6 rats. One group was used as control (3% w/v gum acacia), one as standard(phenytoin), and three groups for the test drug of EECM leaves (doses of 50, 100, and 200 mg/kg) treatment. The reduction in time or abolition of tonicextensor phase of MES-convulsions was recorded for all the animals. In PTZ model, either delay or complete abolition of convulsions in rats treatedwith diazepam and EECM leaves was noted for all the animals.Result: EECM leaves reduced the extensor phase of convulsion in MES in a dose-dependent manner and decrease in the duration of convulsions in PTZmodel with increasing dose. Anticonvulsant activity was seen maximum at the dose of 200 mg/kg.Conclusions: Thus, from the above two seizure models of MES and PTZ, it can be concluded that EECM leaves have got an anticonvulsant effect in anincreasing dose-dependent manner.Keywords: Anticonvulsant, Citrus maximus, Maximal electroshock seizure, Pentylenetetrazol.

EVALUATION OF ANTICONVULSANT ACTIVITY OF MIMOSA PUDICA ROOT LINN IN SWISS ALBINO MICE

&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To evaluate the anticonvulsant activity of ethanolic extract of &lt;em&gt;Mimosa pudica&lt;/em&gt; root (EMPR) in experimental mice models.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods&lt;/strong&gt;&lt;strong&gt;: &lt;/strong&gt;Ethanolic extract of root parts of &lt;em&gt;Mimosa pudica&lt;/em&gt; (EMPR) was prepared by a continuous method using soxhlet apparatus. EMPR in doses of 1000, 2000 mg/kg body wt along with valproate were administrated to albino mice by oral route and anti-epileptic activity was assessed by maximal electroshock (MES) and pentylenetetrazole (PTZ) induced seizure models. Abolition of tonic hind limb extension phase and an increase in seizure latency period, when compared to control group, were taken as a measure of protection in MES and PTZ induced convulsion models respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;EMPR in the dose of 1000 and 2000 mg/kg body wt of mice showed significant anti-epileptic property in both MES and PTZ induced seizure models. There was a significant abolition of tonic hind limb extension phase in MES model. There was also a significant increase in seizure latency period in PTZ induced seizure model.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Results suggest that ethanolic extract of &lt;em&gt;Mimosa pudica&lt;/em&gt; roots possess significant anti-epileptic activity. Further investigations are required to determine its active constituents and also its antiepileptic mechanism of action.&lt;/p&gt;

Anticonvulsant profile of nardostachys jatamansi roots in albino rats

Background: Epilepsy is a common neurological disorder. Despite the massive scale of the problem and much research, epilepsy remains poorly understood. Most of the currently used antiepileptic drugs have some neurotoxic effects, cognitive deficits and teratogenic effects, which decrease their clinical utility and up to 30% of patients are still refractory to treatment. The present study is undertaken to evaluate the anticonvulsant activity of ethanolic extract of nardostachys jatamansi root in albino rats. Methods: Albino rats (150-200 gms) of male sex were randomly selected, from central animal facility. They were divided into 5groups(per model) of 6 rats each, control group-propylene glycol 0.5 ml, standard group-sodium valproate (300 mg/kg), dose 1-ethanolic extract of nardostachys jatamansi roots (100 mg/kg), dose 2-ethanolic extract of nardostachys jatamansi roots (200 mg/kg) and dose 3-ethanolic extract of nardostachys jatamansi roots (400 mg/kg). The anti-convulsant activity was screened using maximal electroshock seizure model and pentylenetetrazole model. Results were analysed by ANOVA followed by post hoc Fisher’s LSD test. Results: The ethanolic extract of nardostachys jatamansi roots at the dose of 400 mg/kg has shown significant anticonvulsant activity in maximal electroshock seizure (MES) model. Whereas, in pentylenetetrazole induced seizure model, the ethanolic extract of nardostachys jatamansi roots has shown significant anticonvulsant activity at the dose of 200 mg/kg and 400 mg/kg body weight. Conclusions: The anticonvulsant activity of ethanolic extract of nardostachys jatamansi roots was less when compared to Sodium Valproate in Maximal Electro Shock model. Whereas, in Pentylenetetrazole induced seizure model, anticonvulsant activity of ethanolic extract of nardostachys jatamansi roots was comparable to sodium valproate.

Evaluation of anticonvulsant activity of ethanolic extract of Momordica tuberosa leaves in experimental animals

Background : The aim of the present study was to evaluate the anticonvulsant activity of ethanolic extract of Momordica tuberosa leaves (EEMTL) in a maximal electric shock induced seizure (MES) model in experimental animals. Methods: A total of 30 albino rats of either sex weighing 150-200 g were randomly divided into five groups of six animals each. Group I received normal saline (0.5 ml), Group II received phenytoin sodium (25 mg/kg body weight) intraperitoneal, Group III, IV, V received different doses (100, 200, 400 mg/kg, respectively) of M. tuberosa leaves extract orally. Convulsions were produced in all groups by giving maximal electric shock of 150 mA for 0.2 sec after 1 hr of giving test and standard drugs. Tonic-clonic seizures were produced after giving an electric shock. Recovery time was noted. The period of tonus, clonus, and stupor were measured and compared between the control, standard and test. Results: In MES model, EEMTLsignificantly (p<0.0001) decreased the duration of tonic-clonic seizures and recovery time. Conclusion: M. tuberosa leaveswere shown anticonvulsant property in MES animal models.

Anti-convulsant activity of ethanolic extract of Moringa concanensis leaves in Swiss albino mice

Objectives : To investigate the anti-convulsant activity of ethanolic extract of Moringa concanensis leaves on maximal electroshock-induced seizures (MES) and pentylenetetrazole (PTZ)-induced seizures in Swiss albino mice. Materials and Methods : The ethanolic extract of Moringa concanensis leaves (200 mg/kg, i.p) was studied for its anti-convulsant effect on MES and PTZ-induced seizures in Swiss albino mice. In MES seizures, suppression of tonic hind limb extension was noted. In PTZ seizures, abolition of the convulsions was noted. Results : The ethanolic extract of the leaves of Moringa concanensis (200 mg/kg, i.p) significantly (P < 0.001) abolished the hind limb extension induced by MES. The same dose also significantly (P < 0.001) protected the animals from PTZ-induced tonic convulsions. Conclusion : The data suggests that the ethanolic extract of Moringa concanensis leaves may produce its anti-convulsant effects via multiple mechanisms since it abolished the hind limb extension induced by MES as well as abolished seizures produced by PTZ.

Evaluation of anticonvulsant activity of ethanolic leaves extract of Desmodium triflorum in mice

The present investigation was aimed to study an anticonvulsant activity of ethanolic extract of Desmodium triflorum (L.) DC., Fabaceae, in mice. Animal models of epilepsy namely the pentylenetetrazole (PTZ), isoniazid or isonicotinic hydrazide (INH) and maximal electroshock induced convulsion (MES) were used to evaluate the anticonvulsant effects of the extracts. The biochemical estimation was done by measuring the lipid peroxidation and reduced glutathione (GSH). In the PTZ induced convulsion, ethanolic extract of D. triflorum (EEDT) 400 mg/kg significant delayed the onset of convulsion, reduced the duration of convulsion and reduced mortality. Similarly a dose of 800 mg/kg of EDDT significantly delayed the onset of convulsion, reduced the duration of convulsion and showed 33.33% protection in mice against INH induced convulsion. Further no mortality was found. Both the doses reduced hind limb tonic extension (HLTE) phase of MES induced convulsion in mice. The pretreated EEDT showed significant inhibition of lipid peroxidation and increases the reduced glutathione level in mice brain tissue. The results revealed that D. triflorum possesses a significant dose dependent anticonvulsant activity.

Anticonvulsant activity of ethanolic extract of Momordica cymbalaria Fenzl. ex Naud

Anticonvulsant activity of ethanolic extract of Momordica cymbalaria Fenzl. ex Naud