Anticlostridial Activity Research Articles

S‐NITROSOCYSTEINE AS AN ANTIOXIDANT, COLOR‐DEVELOPING, AND ANTICLOSTRIDIAL AGENT IN COMMINUTED TURKEY MEAT

ABSTRACTThe effects of S‐nitrosocysteine (RSNO) as an inhibitor of lipid oxidation and as a color‐developing and anticlostridial agent in a comminuted turkey meat product are reported. These effects were compared with those of nitrite on a molecular weight basis. Similar effects were obtained by 25 ppm of nitrite or the corresponding molar concentration of RSNO in color development and inhibition of lipid oxidation in cooked cured meat stored under anaerobic conditions. Transnitrosation between RSNO and hemeproteins occurred in the meat product. Results indicate that as far as the chances for nitrosamines formation are concerned, RSNO cannot be recommended as a substitute for nitrite. In a microbiological medium and in a meat product, RSNO had less anticlostridial activity than nitrite.

Metronidazole Bioassay

Urine from patients receiving metronidazole [1-(2-hydroxyethyl)-2-methyl-5-nitromidazole] orally or per vagina were subjected to paper chromatographic fractionation and examined for anticlostridial activity. Unmodified metronidazole and several metabolites were detected. These findings indicate that the clostridial bioassay may be limited in its applicability to the study of the pharmacodynamics of metronidazole as it does not differentiate between the parent substance and some of its metabolites. Moreover, some of the latter, although they possess antibacterial activity, may not have antiprotozoan activity.