Antibacterial Coatings Research Articles

Development of g-C3N4/ZnO nanocomposite as a novel, highly effective and durable photocatalytic antibacterial coating for cotton fabric

Photocatalytic antibacterial coats are considered among the best solutions to bacterial contamination of fabrics, with the drawback of reduced efficacy after continued use and washing. In the present study, the g-C3N4/ZnO (CNZ) nanocomposite has been introduced as a novel cotton fabric coating, with high durability, and CNZ nanopowders were synthesized using a two-step thermal synthesis process and directly coated onto cotton fabric using the sonication technique. The synthesized nanoparticles (NPs) were examined using X-ray diffraction (XRD), UV–visible spectroscopy, photoluminescence (PL), Brunauer-Emmett-Teller (BET), and Fourier transform infrared (FTIR) analyzes. Besides, the SEM analysis confirmed the successful deposition of NPs on cotton fabric. The photodegradation of methylene blue (MB) stain was assessed as a functional test for the photocatalytic effectiveness of the coated fabric, then its antibacterial properties were evaluated under visible light, by direct contact with bacterial suspensions and culturing. The results revealed that the CNZ-coated cotton fabric containing 30% ZnO (CNZ-30) has significant photocatalytic antibacterial activity against both Escherichia coli (gram-negative), and Staphylococcus aureus (gram-positive) bacteria. The bacterial reduction rate of CNZ-30 coated fabric for both E. coli and S. aureus was above 98%, even after 18 washing cycles. This excellent performance is attributed to the effective coupling of ZnO with g-C3N4, improved light absorption, and reduced e−/h+ pair recombination rates. This study novel coating method can offer an environmentally friendly, cost-effective, and simple process to manufacture hybrid CNZ antibacterial cotton in the textile industry.

Electric Spark Deposition of Antibacterial Silver Coating on Microstructured Titanium Surfaces with a Novel Flexible Brush Electrode.

Infection caused by orthopedic titanium implants, which results in tissue damage, is a key factor in endosseous implant failure. Given the seriousness of implant infections and the limitations of antibiotic therapy, surface microstructures and antimicrobial silver coatings have emerged as prominent research areas and have displayed certain antimicrobial effects. Researchers are now working to combine the two to produce more effective antimicrobial surfaces. However, building robust and homogeneous coatings on complex microstructured surfaces is a tough task due to the limits of surface modification techniques. In this study, a novel flexible electrode brush (silver brush) instead of a traditional hard electrode was designed with electrical discharge machining, which has the ability to adapt to complex groove interiors. The results showed that the use of flexible electrode brush allowed silver to be deposited uniformly in titanium alloy microgrooves. On the surface of Ag-TC4, a uniformly covered deposit was visible, and it slowly released silver ions into a liquid environment. In vitro bacterial assays showed that a Ag-TC4 microstructured surface reduced bacterial adhesion and bacterial biofilm formation, and the antibacterial activity of Ag-TC4 against Staphylococcus aureus and Escherichia coli was 99.68% ± 0.002 and 99.50% ± 0.007, respectively. This research could lay the groundwork for the study of antimicrobial metal bound to microstructured surfaces and pave the way for future implant surface design.

Physically crosslinked PAA/Lys-BPEA hydrogel with rapid self-healing and long-term antibacterial activities

Diseases caused by bacterial infections continue to threaten human health around the world. Antimicrobial hydrogels with excellent properties have been developed in biomaterial and other fields in some cases, where overuse or inappropriate use of antibiotics has led to increased bacterial resistance. In this study, primary amines that are rich in lysine end groups were used to synthesize Lys-BPEA crosslinker. An antibacterial self-healing hydrogel (labeled as polyacrylic acid [PAA/]/Lys-BPEA) was prepared by introducing polyamine Lys-BPEA into PAA via ionic bonding and electrostatic interaction. The surface morphology and mechanical properties, especially the antibacterial and self-healing properties, of the created physically crosslinked PAA-lysine polyetheramide hydrogels were investigated. The PAA/Lys-BPEA hydrogel realized a comprehensive performance of long-term antibacterial activity without inducing drug resistance, low hemolysis activity and rapid self-healing ability. Therefore, the performance of the combination makes the PAA/Lys-BPEA hydrogel an ideal candidate for the design of smart self-healing devices and antibacterial surface coatings.

The oxidation state of Ag nanoparticles highly affects the release of Ag ions without compromising the mechanical performance and the safety of amorphous hydrogenated carbon coatings

Antibacterial coatings play an important adjunct against hospital-acquired infections. More specifically, the use of silver nanoparticles (Ag NPs) incorporated on amorphous hydrogenated carbon (a-C:H) demonstrates a promising approach to reduce microbial contamination while maintaining excellent mechanical properties. However, their success as a long-term (e.g. years) antibacterial coating hinges on the control over Ag+ release. In this sense, if a continuous release is required, a driving force must exist to induce silver ionization. Thus, this research studies the influence of silver oxide (AgO) NPs in a-C:H film as a novel and a simple approach to activate the release of Ag+. Herein, Ag and AgO NPs were deposited on a-C:H films (with 2.8 and 2.5 at.% Ag, respectively) using low-pressure plasma. Their mechanical properties, release kinetics, and antibacterial activity were analyzed, with special attention to cell viability. Overall, a-C:H:Ag showed an increase in hardness, whereas a-C:H:AgO exhibited ameliorated mechanical properties with higher elastic deformation and wear resistance. Moreover, the coatings did not induce any cytotoxicity after 7 days. Regarding the release kinetics, a-C:H:Ag displayed a slow-release reaching a maximum of 22 μg/L, while a-C:H:AgO showed a fast continuous-release with a maximum of 148 μg/L. In this sense, a-C:H:AgO exhibited improved antibacterial activity in comparison with a-C:H:Ag. The use of AgO NPs in a-C:H film demonstrated to be a simple approach to activate the release of Ag+, without compromising the mechanical and the cytotoxic properties of the coating. These results highlight a new pathway towards coatings with lasting antibacterial and mechanical stability.

Smart composite antibacterial coatings with active corrosion protection of magnesium alloys

A new method of the formation of composite coatings with the function of active corrosion protection of magnesium alloys was developed using the plasma electrolytic oxidation (PEO) method. Susceptibility of PEO-layers to pitting formation was evaluated using localized electrochemical methods (SVET/SIET). The morphological features and electrochemical properties of composite coatings were studied using SEM/EDX, XRD, micro-Raman spectroscopy and EIS/PDP measurements, respectively. The effect of surface layers impregnation with corrosion inhibitor on their protective properties in a corrosive environment was established. Additional protection was achieved using controllable coating pore sealing with polymer. It was found that the polymer treatment of the PEO-layer does not reduce the inhibitor's efficiency. The formed protective composite inhibitor-and-polymer-containing layers decrease the corrosion current density of a magnesium alloy in a 3 wt.% NaCl solution to three orders of magnitude. This predetermines the prospect of new smart coatings formation that significantly expand the field of application of electrochemically active materials. The mechanism of smart composite coating corrosion degradation was established. The antibacterial activity of the inhibitor-containing coatings against S. aureus methicillin-resistant strain was proved using the in vitro model. These protective layers are promising for reducing the incidence of implant-associated infections.

Antibacterial Coatings for Titanium Implants: Recent Trends and Future Perspectives.

Titanium and its alloys are widely used as implant materials for biomedical devices owing to their high mechanical strength, biocompatibility, and corrosion resistance. However, there is a significant rise in implant-associated infections (IAIs) leading to revision surgeries, which are more complicated than the original replacement surgery. To reduce the risk of infections, numerous antibacterial agents, e.g., bioactive compounds, metal ions, nanoparticles, antimicrobial peptides, polymers, etc., have been incorporated on the surface of the titanium implant. Various coating methods and surface modification techniques, e.g., micro-arc oxidation (MAO), layer-by-layer (LbL) assembly, plasma electrolytic oxidation (PEO), anodization, magnetron sputtering, and spin coating, are exploited in the race to create a biocompatible, antibacterial titanium implant surface that can simultaneously promote tissue integration around the implant. The nature and surface morphology of implant coatings play an important role in bacterial inhibition and drug delivery. Surface modification of titanium implants with nanostructured materials, such as titanium nanotubes, enhances bone regeneration. Antimicrobial peptides loaded with antibiotics help to achieve sustained drug release and reduce the risk of antibiotic resistance. Additive manufacturing of patient-specific porous titanium implants will have a clear future direction in the development of antimicrobial titanium implants. In this review, a brief overview of the different types of coatings that are used to prevent implant-associated infections and the applications of 3D printing in the development of antibacterial titanium implants is presented.

Composite Coating for the Food Industry Based on Fluoroplast and ZnO-NPs: Physical and Chemical Properties, Antibacterial and Antibiofilm Activity, Cytotoxicity

Bacterial contamination of meat products during its preparation at the enterprise is an important problem for the global food industry. Cutting boards are one of the main sources of infection. In order to solve this problem, the creation of mechanically stable coatings with antibacterial activity is one of the most promising strategies. For such a coating, we developed a composite material based on "liquid" Teflon and zinc oxide nanoparticles (ZnO-NPs). The nanoparticles obtained with laser ablation had a rod-like morphology, an average size of ~60 nm, and a ζ-potential of +30 mV. The polymer composite material was obtained by adding the ZnO-NPs to the polymer matrix at a concentration of 0.001-0.1% using the low-temperature technology developed by the research team. When applying a composite material to a surface with damage, the elimination of defects on a micrometer scale was observed. The effect of the composite material on the generation of reactive oxygen species (H2O2, •OH), 8-oxoguanine in DNA in vitro, and long-lived reactive protein species (LRPS) was evaluated. The composite coating increased the generation of all of the studied compounds by 50-200%. The effect depended on the concentration of added ZnO-NPs. The antibacterial and antibiofilm effects of the Teflon/ZnO NP coating against L. monocytogenes, S. aureus, P. aeruginosa, and S. typhimurium, as well as cytotoxicity against the primary culture of mouse fibroblasts, were studied. The conducted microbiological study showed that the fluoroplast/ZnO-NPs coating has a strong bacteriostatic effect against both Gram-positive and Gram-negative bacteria. In addition, the fluoroplast/ZnO-NPs composite material only showed potential cytotoxicity against primary mammalian cell culture at a concentration of 0.1%. Thus, a composite material has been obtained, the use of which may be promising for the creation of antibacterial coatings in the meat processing industry.

Invisible Bactericidal Coatings on Generic Surfaces through a Convenient Hand Spray.

Robust antimicrobial coatings featuring high transparency, strong bactericidal activity, and an easy application procedure on generic surfaces can be widely accepted by the public to prevent pandemics. In this work, we demonstrated the hand-sprayer-based approach to deposit complex oxide coatings composed of Co-Mn-Cu-Zn-Ag on screen protectors of smartphones through acidic redox-assisted deposition (ARD). The as-obtained coatings possess high transparency (99.74% transmittance at 550 nm) and long-lasting durability against swiping (for 135 days of average use) or wet cleaning (for a routine of 3 times/day for 33 days). The spray coating enabling 3.14% Escherichia coli viability can further be reduced to 0.21% through a consistent elemental composition achieved via the immersion method. The high intake of Cu2+ in the coating is majorly responsible for the bactericidal activity, and the presence of Ag+ and Zn2+ is necessary to achieve almost complete eradication. The success of extending the bactericidal coatings on other typical hand-touched surfaces (e.g., stainless steel railings, rubber handrails, and plastic switches) in public areas has been demonstrated.

Surface Modification of 3D-Printed PCL/BG Composite Scaffolds via Mussel-Inspired Polydopamine and Effective Antibacterial Coatings for Biomedical Applications.

A wide variety of composite scaffolds with unique geometry, porosity and pore size can be fabricated with versatile 3D printing techniques. In this work, we fabricated 3D-printed composite scaffolds of polycaprolactone (PCL) incorporating bioactive glass (BG) particles (13-93 and 13-93B3 compositions) by using fused deposition modeling (FDM). The scaffolds were modified with a "mussel-inspired surface coating" to regulate biological properties. The chemical and surface properties of scaffolds were analyzed by Fourier transform infrared spectroscopy (FTIR), contact angle and scanning electron microscopy (SEM). Polydopamine (PDA) surface-modified composite scaffolds exhibited attractive properties. Firstly, after the surface modification, the adhesion of a composite coating based on gelatin incorporated with strontium-doped mesoporous bioactive glass (Sr-MBGNs/gelatin) was significantly improved. In addition, cell attachment and differentiation were promoted, and the antibacterial properties of the scaffolds were increased. Moreover, the bioactivity of these scaffolds was also significantly influenced: a hydroxyapatite layer formed on the scaffold surface after 3 days of immersion in SBF. Our results suggest that the promoting effect of PDA coating on PCL-BG scaffolds leads to improved scaffolds for bone tissue engineering.

Hyaluronic acid-based hydrogel coatings on Ti6Al4V implantable biomaterial with multifunctional antibacterial activity

Today, the treatment of implant-associated infections with conventional mono-functional antibacterial coatings has not been effective enough for a prosperous long-term implantation. Therefore, biomedical industry is making considerable efforts on the development of novel antibacterial coatings with a combination of more than one antibacterial strategies that interact synergistically to reinforce each other. Therefore, in this work hyaluronic acid-based (HA) hydrogel coatings were created on the surface Ti6Al4V biomaterial with 1,4-butanediol diglycidyl ether (Ti-HABDDE) and divinyl sulfone (Ti-HADVS) crosslinking agents. Hydrogel coatings displayed an extraordinary in vivo biocompatibility, a remarkable ability to promote cell proliferation, differentiation and mineralization, and capability to sustainedly release drugs. Finally, HA-based hydrogel coatings demonstrated an outstanding multifunctional antibacterial activity: bacteria-repelling (51–55 % of S. aureus and 27–40 % of E. coli), bacteria-killing (82–119 % of S. aureus and 83–87 % of E. coli) and bactericide release killing (drug-loaded hydrogel coatings, R > 2).

Antibacterial brush polypeptide coatings with anionic backbones

Preventing initial colonization of bacteria on biomaterial surfaces is crucial to address the medical device-associated infection issues. Antimicrobial peptide (AMP) or cationic polymer modified surfaces have shown promising potentials to inhibit the initial colonization of bacteria by contact killing. However, their development has been impeded because of bacterial adhesion and high cytotoxicity. Herein, we report a series of brush polypeptide coatings with anionic backbones and cationic AMP mimetic side-chains that displayed superior bactericidal activity, antibacterial adhesion property, and biocompatibility. The cationic side-chain density played an important role in the bioactivities of the brush polypeptide modified surfaces. Brush polypeptide coating with low side-chain density exhibited improved bactericidal activity and antibacterial adhesion property, ascribing to the cooperative effects of adjacent side-chains and backbones/side-chains, respectively. It also showed negligible hemolysis/cytotoxicity in vitro and potent anti-infection property (≥99.9% bactericidal efficacy) in vivo. Brush polymers with anionic backbones and cationic side-chains can be used as a promising design motif to potentiate both antibacterial property and biocompatibility of coatings for combating device-associated infections. Statement of significanceDevice-associated infections (DAIs) have led to increased medical cost, pain, and even mortality of patients. Antimicrobial peptide and cationic polymer coatings provide an important strategy to combat DAIs by preventing initial colonization of bacteria on biomaterial surfaces. Nevertheless, they have suffered bacterial adhesion and cytotoxicity issues. Herein, we developed a brush polypeptide coating with anionic backbones and cationic side-chains. The brush polypeptide coating showed superior bactericidal and antibacterial adhesion properties outperforming conventional antibacterial coatings based on antimicrobial peptide (i.e., melittin), lysozyme (i.e., lysostaphin), cationic polymer, anionic polymer, and the blends of cationic/anionic polymers. It also showed good biocompatibility and potent anti-infection property, making it a promising candidate to combat the DAIs.

Straightforward Approach for Preparing Durable Antibacterial ZnO Nanoparticle Coatings on Flexible Substrates.

Flexible antibacterial materials have gained utmost importance in protection from the distribution of bacteria and viruses due to the exceptional variety of applications. Herein, we demonstrate a readily scalable and rapid single-step approach for producing durable ZnO nanoparticle antibacterial coating on flexible polymer substrates at room temperature. Substrates used are polystyrene, poly(ethylene-co-vinyl acetate) copolymer, poly(methyl methacrylate), polypropylene, high density polyethylene and a commercial acrylate type adhesive tape. The deposition was achieved by a spin-coating process using a slurry of ZnO nanoparticles in toluene. A stable modification layer was obtained when toluene was a solvent for the polymer substrates, namely polystyrene and poly(ethylene-co-vinyl acetate). These coatings show high antibacterial efficiency causing >5 log decrease in the viable counts of Gram-negative bacteria Escherichia. coli and Gram-positive bacteria Staphylococcus aureus in 120 min. Even after tapping these coated surfaces 500 times, the antibacterial properties remained unchanged, showing that the coating obtained by the presented method is very robust. In contrast to the above findings, the coatings are unstable when toluene is not a solvent for the substrate.

Attapulgite Doped with Fe and Cu Nanooxides as Peroxidase Nanozymes for Antibacterial Coatings

Attapulgite Doped with Fe and Cu Nanooxides as Peroxidase Nanozymes for Antibacterial Coatings

Recent Advance in Polymer Coatings Combating Bacterial Adhesion and Biofilm Formation†

Comprehensive SummaryImplantable medical device‐associated infections (DAIs) originating from bacterial adhesion and biofilm formation have threatened to the health and life of patients. Antibacterial polymer coatings with antifouling and/or bactericidal properties have showed great potentials to combat DAI issues. In this review, we report recent advances in antibacterial polymer coatings fighting bacterial adhesion and biofilm formation on implantable biomaterial surfaces. We summarize the mechanisms of bacterial adhesion and biofilm formation, which provides guidance for the design of antibacterial coatings. We describe the polymer and coating preparation methods and discuss the structure‐property relationships of antibacterial polymer coatings. Applications of these polymer coatings in medical catheters, orthopaedic implants, and other applications are elaborated. Future challenges and prospects associated with antibacterial polymer coatings for implantable medical devices are discussed.

Multifunctional antifogging, self-cleaning, antibacterial, and self-healing coatings based on polyelectrolyte complexes

In this study, a multifunctional coating with antifogging, self-cleaning, antibacterial, and self-healing capabilities is developed based on polyelectrolyte complexes of polyethylenimine (PEI), phytic acid (PA), and Cu2+ (denoted as PEI-PA-Cu). The multifunctional PEI-PA-Cu coating with high transparency can be prepared by spraying, brushing, or other one-step deposition onto various substrates (e.g., polymeric substrates and glass slides). Such a coating shows an excellent antifogging effect in both high temperature (85 ℃) and cold environments (−20 ℃) and maintains stable antifogging performance after placing in ∼65 ℃ vapor for 9 h. In addition, the coating is superhydrophilic and allows for easy spreading of water to isolate the oily contaminants, realizing self-cleaning. Meanwhile, the coating possesses superior antibacterial capability against Escherichia coli and Staphylococcus aureus thanks to PEI with amino groups and Cu2+. Importantly, the PEI-PA-Cu coating can heal damages rapidly and repeatedly due to the presence of hydrogen bonds and electrostatic interactions, effectively prolonging the service life. This study provides a facile route to construct multifunctional superhydrophilic coatings with promising prospects in the field of personal protective, medical, and optical devices.

Green in-situ synthesis of silver coated textiles for wide hygiene and healthcare applications

In this study, antibacterial and biocompatible silver-based (Ag0) coatings on cotton fabric were obtained by using atmospheric pressure plasma mediated in situ silver reduction, an effective, facile, and environment-friendly technique. The formation of the thin (nanometers in thickness) Ag0 coating on the fabric fibers was characterized by scanning electron microscopy (SEM) and backscattered electron (BSE) analysis. In particular, the antibacterial activity demonstrated by the silver-fabrics (Ag0-fabrics) against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria was about 105 times more potent than silver ion or silver nanoparticle fabricated fabrics. The Ag0-fabrics also demonstrated good killing activity to preformed biofilms. Moreover, because of the greatly reduced silver loading, Ag0-fabrics did not pose significant cytotoxicity to human tissue cells exhibiting good biocompatibility. Given the economic and environment-friendly nature of the Ag0-fabrics technology wide applications can be derived in health, hygiene, and medical fields.

Antibacterial and Photocatalytic Coatings Based on Cu-Doped ZnO Nanoparticles into Microcellulose Matrix.

The paper presents a successful, simple method for the preparation and deposition of new hybrid Cu-doped ZnO/microcellulose coatings on textile fibers, directly from cellulose aqueous solution. The morphological, compositional, and structural properties of the obtained materials were investigated using different characterization methods, such as SEM-EDX, XRD, Raman and FTIR, as well as BET surface area measurements. The successful doping of ZnO NPs with Cu was confirmed by the EDX and Raman analysis. As a result of Cu doping, the hybrid NPs experienced a phase change from ZnO to (Zn0.9Cu0.1)O, as shown by the XRD results. All the hybrid NPs exhibited a high degree of crystallinity, as revealed by the very sharp reflections in XRD patterns and suggested also by the Raman results. The evaluation of the very low copper-doping (0.1-1 at.%) effect has shown different behavior trends of the hybrid coatings compared with the starting oxide NPs, for MB and MO photodegradation. Continuous increases up to 92% and 60% for MB and MO degradation, respectively, were obtained at maximum 1 at.%-Cu doping coatings. Strong antibacterial activity against S. aureus and E. coli were observed.

Antibacterial Edible Coating of Ipomea batatas Incorporated with Lactobacillus acidophilus

This study was conducted to analyze the effect of variations concentration of Lactobacillus acidophilus in edible coatings and their antibacterial activity against E. coli using the well diffusion method. This study used an edible coating of Prebiotic from purple sweet potato starch and Lactobacillus acidophilus with dilution variations (10-6, 10-7, 10-8, 10-9) as probiotics. In this study physical properties were tested for edible coatings such as color tests with visual observations, water content by the gravimetric method and viscosity using Viscometer Ostwald. The content of organic acids in edible coating solutions was measured using the HPLC method. Organoleptic test was conducted with taste and color parameters on the A-E scale of 30 respondents. Research results showed edible coating discoloration before and after incorporation from deep purple to brown. The value of water content and viscosity also changed from 62,8% to 71,4% and 569,97 cp to 486,64 cp respectively. The best antibacterial activity of edible coating incorporation with Lactobacillus acidophilus was 263,76 mm2. Organoleptic test on grapes that have been coated with edible coating showed that covered with edible coating contains probiotics (Lactobacillus acidophilus) has no effect on respondents' perception. Lactic acid and acetic acid were exist in edible coating which were incorporated with Lactobacillus acidophilus.

Cellulose Nanofibers/Pectin/Pomegranate Extract Nanocomposite as Antibacterial and Antioxidant Films and Coating for Paper.

Bio-based polymer composites find increasing research and industrial interest in different areas of our life. In this study, cellulose nanofibers (CNFs) isolated from sugar beet pulp and nanoemulsion prepared from sugar beet pectin and pomegranate extract (PGE) were used for making films and used as coating with antioxidant and antimicrobial activities for paper. For Pectin/PGE nanoemulsion preparation, different ratios of PGE were mixed with pectin using ultrasonic treatment; the antibacterial properties were evaluated to choose the formula with the adequate antibacterial activity. The antioxidant activity of the nanoemulsion with the highest antimicrobial activity was also evaluated. The nanoemulsion with the optimum antibacterial activity was mixed with different ratios of CNFs. Mechanical, greaseproof, antioxidant activity, and antibacterial properties of the CNFs/Pectin/PGE films were evaluated. Finally, the CNFs/Pectin/PGE formulation with the highest antibacterial activity was tested as a coating material for paper. Mechanical, greaseproof, and air porosity properties, as well as water vapor permeability and migration of the coated layer from paper sheets in different media were evaluated. The results showed promising applicability of the CNFs/Pectin/PGE as films and coating material with antibacterial and antioxidant activities, as well as good stability for packaging aqueous, fatty, and acidic food products.

Using a degradable three-layer sandwich-type coating to prevent titanium implant infection with the combined efficient bactericidal ability and fast immune remodeling property

Titanium (Ti) implant-associated infections are a challenge in orthopedic surgery, for which a series of antibacterial coatings have been designed and fabricated to reduce the risk of bacterial contamination. Herein, we created a degradable three-layer sandwich-type coating to achieve long-term antibacterial effects while simultaneously reconstructing the local immune microenvironment. The vancomycin (Van)-loaded vaterite coating constitutes the outer and inner layers, whereas Interleukin-12 (IL-12)-containing liposomes embedded in sodium alginate constitutes the middle layer. Van, released from the vaterite, demonstrated a favorable and rapid bactericidal ability against the representative methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) strains. The released IL-12 exhibited the desired immune reconstitution abilities, actively facilitating defenses against subsequent bacterial invasions. Furthermore, the biocompatibility and cell-binding feature of the multifunctional coating was beneficial for achieving solid interface intergradation. Overall, the benefits of the three-layer sandwich-type coating, including the convenient fabrication process, efficient antimicrobial activity, fast immune remodeling property, fine cell-binding feature, and biodegradability, highlight its promising translational potential in preventing implant infection. Statement of significanceTo prevent titanium implant infections, researchers have designed various antibacterial coatings. However, most of these coatings focused only on killing the invading bacteria over a limited postoperative period. However, the local immune microenvironment is compromised during surgery. Local immune deflection impedes the ability of the local immune defenses to clear bacteria and limits immune memory building from active defense against long-term subsequent bacterial invasions. Furthermore, these coatings are usually nondegradable and differ substantially from bone components, thereby impairing the integration of the coating and bone interface and generating concerns about implant stability and bacterial contamination. In this work, we synthesized a degradable coating that provides sustained antibacterial activity, promotes immune reconstitution, and simultaneously achieves solid bone integration.