Anti-aging Products Research Articles

Biosurfactants as determinants of the quality anti-aging products

Abstract Analysing the possibilities of obtaining biosurfactants and using waste materials as substrates for their production allows us to emphasize that biosurfactants are in line with the ideas of sustainable development and the protection of non-renewable resources. These ideas also apply to the engineering and technology of cosmetic products. For this reason, and because of the multi-functionality of biosurfactants, their role and widespread use in cosmetic formulations is likely to increase. Biosurfactants tend to have good surface properties. Their structural diversity results in differences in biological, antioxidant or biocidal activity. The paper presents the potential of biosurfactants that could be used in the composition of products dedicated to the fight against the problems of ageing skin. Particularly important in the skin ageing process is the fight against radicals and the prevention of collagen loss in the skin. The overall appearance of the skin also depends on the level of hydration and roughness. Biosurfactants also have an effect on the melanogenesis process, controlling hyperpigmentation. A relatively new approach to the fight against skin ageing emphasizes the role of the microbiome, and available research also points to the correlation of biosurfactants with maintaining the correct structure of the microbiome.

Tocopherol succinate-loaded ethosomal gel synthesized by cold method technique: Deeper biophysical characterizations for translational application on human skin.

Tocopherols are well-known antioxidant and moisturizing agent. Tocopherol succinate (TS) are widely used in many skin products especially used in anti-aging and skin whitening product formulation. We previously reported the successful synthesis and preliminary characterizations of stable TS ethosomal gels (TSEG) (DOI: 10.1111/jocd.14907). Herein, we develop and further characterize TSEG to enhance the stability of the developed formulation with increased permeation through skin. Cold method technique was used to prepare TS ethosomes. The developed ethosomal vesicle size was 250 nm, which allowed TS to penetrate through the stratum corneum layer and act on melanocytes. For stability study was assessed by thermogravimetric analysis (TGA) by placing TSEG and unloaded/control ethosomal gel (CEG) at various temperature conditions, that is, 8°C, 25°C, 40°C, and 40°C ± 75% RH for 3 months. Organoleptic evaluation was done in terms of color, odor, and phase separation. Transmission electron microscopy (TEM), Fourier Transform infrared spectroscopy (FTIR), x-ray diffraction spectroscopy (XRD), zeta potential (ZP) and particle size (PS) was used for TSEG physical characterizations. Invitro dissolution and ex-vivo permeation studies (using Franz diffusion cell) were performed for both TSEG and CEG formulations. Human women (N = 34) were used to evaluate invivo biophysical parameters including erythema, melanin, moisture content, sebum level, and skin elasticity. Developed formulation was highly thermostable during the 3 months. Erythema, melanin, and sebum level decreased while marked improvement (p < 0.05) in moisture content and elasticity have been observed for the developed TSEG. The developed TSEG formulation was found to be efficient, safe (no adverse effects observed), stable (at least for 3 months), and easy to use for topical application with improved skin complexation and skin integrity.

Antiaging Strategies and Remedies: A Landscape of Research Progress and Promise.

Aging is typified by a gradual loss of physiological fitness and accumulation of cellular damage, leading to deteriorated functions and enhanced vulnerability to diseases. Antiaging research has a long history throughout civilization, with many efforts put forth to understand and prevent the effects of aging. Multiple strategies aiming to promote healthy aging and extend the lifespan have been developed including lifestyle adjustments, medical treatments, and social programs. A multitude of antiaging medicines and remedies have also been explored. Here, we use data from the CAS Content Collection to analyze the publication landscape of recent research related to antiaging strategies and treatments. We review the recent advances and delineate trends in research headway of antiaging knowledge and practice across time, geography, and development pipelines. We further assess the state-of-the-art antiaging approaches and explore their correlations with age-related diseases. The landscape of antiaging drugs has been outlined and explored. Well-recognized and novel, currently evaluated antiaging agents have also been summarized. Finally, we review clinical applications of antiaging products with their development pipelines. The objective of this review is to summarize current knowledge on preventive strategies and treatment remedies in the field of aging, to outline challenges and evaluate growth opportunities, in order to further efforts to solve the problems that remain.

Clinical Safety and Efficacy Evaluation of a Dissolving Microneedle Patch Having Dual Anti-Wrinkle Effects With Safe and Long-Term Activities.

Anti-aging products are widely used, but the desire for safe and more efficient anti-aging products continues to increase. Dissolving microneedle patches (MNPs) have provided a more efficient transdermal drug delivery solution. MNP is a promising candidate for developing better anti-aging products. To develop a more efficient anti-aging MNP product, we fabricated a dual anti-wrinkle microneedle patch (named DA-MNP) using droplet extension (DEN®) technology and evaluated its skin puncture ability, safety, and efficacy through clinical studies. A DA-MNP comprising hyaluronic acid (HA) polymer backbone, acetyl octapeptide-3, and L-ascorbic acid 2-glucoside and sodium cyclic lysophosphatidic acid was fabricated using DEN® technology. Placebo MNPs comprising only HA were also fabricated. Twenty-four healthy subjects were enrolled in this comparative clinical study. The DA-MNP or placebo MNP was separately applied to the left and right eyes of subjects for overnight. Assessments, including wrinkle improvement, trans-epidermal water loss (TEWL), eye lifting and adverse effects were evaluated at each scheduled visit day for 28 days. The DA-MNP showed mechanical strength enough for puncturing the stratum corneum. Compared to placebo MNP group, the DA-MNP treated group showed an effective eye wrinkles improvement and better anti-aging of skin, with reduced TEWL, enhanced skin elasticity and lifting, and no adverse effects. The present study demonstrated that the fabricated DA-MNP exhibited fast acting on deep wrinkles and enhanced anti-aging efficacy, with no skin safety concern. Thus, this DA-MNP may serve as a new transdermal delivery solution for skin wrinkling and aging.

The efficacy of antiaging cream with Oxalis dehradunensis R. ethanolic extract as skin aging care among woman agriculture workers.

Skin damage and aging are potential health problems for woman agriculture workers. This study aimed to test the efficacy of Oxalis dehradunensis ethanol extract formulated in antiaging cream preparations as an aging treatment in women agriculture workers. The method carried out was an experimental study on woman agriculture workers who were willing to volunteer. The experimental scenario conducted related to the physical quality of antiaging cream products and the efficacy of creams on the skin as an antiaging treatment. Physical quality parameters of antiaging cream include organoleptic assessment, cream emulsion, homogeneity, viscosity, pH, distribution, and skin irritation test to evaluate potential side effects. Skin aging efficacy assessments were conducted on 12 subjects divided into four formula concentration groups. The physical skin identification parameters measured are moisture, pore size, pigmentation or spots, and wrinkles using a skin analyzer. The results found that O. dehradunensis leaf extract formulated as an antiaging cream can neutralize free radicals and is an effective countermeasure against premature skin aging. There were significant differences in the skin characteristics of woman agriculture workers who participated as samples. The formula with 5% concentrate and 7% extract of O. dehradunensis has provided a reaction and is more effective in continuous treatment. It provides skin moisture changes of more than 300%, disguises pore size and good pigmentation, and reduces wrinkles of farmers who are constantly exposed to chemicals and free radicals in their agricultural activities. The leaf extracts antiaging cream showed more significant changes in moisture and skin pigmentation. It was concluded that the use of O. dehradunensis leaf extract as the core ingredient of antiaging cream can be an innovation that is beneficial to the health of the farming community, especially among women agriculture workers.

Sacha Inchi: The Promising Source of Functional Oil for Anti-Aging Product.

Sacha inchi (Plukenetia volubilis) oil is constituted with macronutrients and the health benefit fatty acids. In this context, the efficient of Sacha inchi oil for anti-aging product is presented. The light-clear yellowish seed oil of Sacha inchi was revealed on its physicochemical properties that are in the same range of the commercializing plant-oil supplied for topical products. The oil was GC/MS exhibited to be constituted with α-linolenic (51.72%) and linoleic (24.3%) acids, with unsaturated/saturated fatty acids ratio of 21.26. The oil was noted onto its potent in vitro antioxidant activity assessed by ABTS, DPPH and FRAP assays. In addition, the oil (1-3%) was proved to be safe in normal human fibroblast cells. Furthermore, the oil exhibited cellular antioxidant with inhibitory effect against MMP-2. Sacha inchi oil is therefore highlighted as a potential source of nutraceutical especially for anti-aging product. The oil is specified for the product development in terms of physicochemical, chemical and biological profiles. Innovative processing of Sacha inchi is therefore encouraged as the promising plant for anti-aging product.

The role of the ImmunatuRNA® complex in promoting skin immunity and its regenerative abilities: Implications for antiaging skincare.

Recent advancements in cosmetic science have ushered in a new era of skincare strategies, with a focus on utilizing natural bioactive ingredients to enhance skin health and combat premature aging. The skin, as the largest organ of human body, provides as a vital protective barrier against external hazards such as environmental pollutions, toxins, and radiation. However, intrinsic and extrinsic factors, including various types of radiation, reduced air quality, and increased exposure to pollutants, lead to an imbalance in the skin's immune system, significantly reducing the skin's ability to regenerate and accelerating skin aging. Therefore, there is an emerging need to develop innovative skincare strategies that could support the skin's immune capacity by strengthening antioxidant protection, skin regeneration, and repair. Plant-derived compounds, along with naturally sourced ingredients, show promise in accelerating wound healing, especially when incorporated into cosmetic formulation. ImmunatuRNA® stands as a prime example of a biologically active complex, uniquely comprising yeast-derived RNA, marine exopolysaccharides, and natural hyaluronic acid, that exhibits high antioxidant activity and exerts beneficial modulatory effects on skin microbiota, thereby positively influencing skin immunity. The main aim of this study was to investigate the potential of the ImmunatuRNA® complex in promoting skin regeneration and reducing signs of skin aging, both through the use of invitro human skin cultures and the evaluation of clinical trials in healthy volunteers. The results of conducted experimental studies have shown that the ImmunatuRNA® complex demonstrated significant positive effects on the immunity and repair capabilities of the skin, characterized by increased fibroblast proliferation, enhanced glycosaminoglycan synthesis, and reduced oxidative stress. Furthermore, use of the complex also significantly accelerated wound healing following mechanically-induced damage in the keratinocytes, demonstrated as reduction in wound margins measurement, new cell production, and an increase in regeneration speed. In addition, conduced clinical study on healthy human volunteers with various skin types confirmed that use of cosmetic products that incorporate the ImmunatuRNA® complex within the formulation can visibly improve skin condition, appearance, and general health, achieved by increased skin hydration and elasticity, reduced wrinkles, and enhanced skin firmness. This study confirms the usefulness of the ImmunatuRNA® complex in the innovative antiaging cosmetic products that can be suitable for all skin types, including sensitive skin. The inclusion of naturally sourced bioactives, as those found in ImmunatuRNA® complex, represents a promising advancement in holistic natural skincare that consumers appreciate. The active ingredients of the complex support the skin's immunity, fostering its repair and protecting against oxidative damage, thus maintaining skin homeostasis and promoting its regenerative capacity. Further research is necessary to explore the long-term effects of ImmunatuRNA® complex on skin health and its potential applications in innovative skincare formulations.

Antioxidant and anti-aging effects of purified Rehmannia glutinosa polysaccharide in Caenorhabditis elegans

Oxidative stress has been linked to the onset and progression of aging. Natural polysaccharides have attracted considerable attention in the field of antioxidants and anti-aging products owing to their exceptional biological activity and low toxicity. In this study, a homogeneous (glucogalactan-structured) polysaccharide (RGP70–1) was purified from the roots of Rehmannia glutinosa Libosch. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), monosaccharide composition, methylation, and nuclear magnetic resonance analyses were employed to characterize structure. RGP70–1 (3.6 kDa) was composed of glucose, galactose. RGP70–1's primary chain consisted of α-D-Galp connected by 1,4-linkages, α-D-Galp connected by 1,6-linkages, and β-D-Galp connected by 1,3,4,6-linkages.The side branches included α-D-Galp linked in a 1,4 position, α-D-Galp linked in a 1,6 position, and α-D-Glcp linked at the terminal end. In vivo, the effects of RGP70–1 on aging were examined using a Caenorhabditis elegans model. The findings demonstrated that RGP70–1 enhanced the performance of antioxidant enzymes, decreased reactive oxygen species levels, and prolonged the lifespan of Caenorhabditis elegans. The results of the quantitative real-time polymerase chain reaction indicated that RGP70–1 might demonstrate anti-aging properties by influencing insulin/insulin-like signaling pathway. In general, these findings highlight the possibility of RGP70–1 being utilized in dietary supplements for its anti-aging properties.

Synthesis of enamine derivatives bearing of 2H-pyran-2,4(3H)-dione moiety from dehydroacetic acid: A promising frontier in skin protection and anti-aging, in-vitro, and in-silico insights

In aging research, the most crucial objective is to find longevity-enhancing drugs and analyze their subsequent mode of action. Search for longevity-enhancing drugs and their targets is, however, very challenging. The current study aimed to synthesize novel enamine-based derivatives as anti-aging products. The structures of these derivatives were characterized using multinuclear nmR (1H, 13C, and DEPT-135), mass spectrometry, single crystal XRD, and elemental analysis. Almost all the derivatives 3a-g showed good to excellent activities against elastase enzyme more than the reference drug. The IC50 value of compounds 3e and 3f (0.8 ± 0.2 µM and 0.7 ± 0.4 µM resp.) make them the most potent derivatives against elastase enzyme. Compound 3f exhibited prominent free radical scavenging activity (95.7%) and showed a zone of inhibition (24 mM/100 µL) against candida albicans. The docking studies showed that derivative 3b has the lowest binding affinity (-8.8 kcal/mol) and the best fitting orientation in the active pocket of the elastase enzyme. We infer from the biological activity results that some derivatives can be skin-protecting agents.

Comparative Study of Liposomal and Ethosomal Formulations of Curcuma heyneana Rhizome Extract in a Transdermal Delivery System.

This study aimed to develop an anti-aging nanoformulation with Curcuma heyneana extract as bioactive substance. Curcuma heyneana Valeton & Zipj extract has been proven in previous research to have antioxidant, anti-ageing, anti-inflammatory, and wound healing properties, which makes it a potential bioactive material for anti-ageing and sunscreen cosmetic products. Phytoantioxidants need to penetrate into deeper skin layers to ensure effectivity. Thus, a transdermal delivery system is needed to deliver the extract to a deeper skin layer. The objective of the study was to compare the permeability and anti-ageing activity of liposomal and ethosomal formulations of C. heynena rhizome ethanolic extract. In this study, C. heyneana extract was loaded into a phospholipid vesicular system in the form of liposome and ethosome formulations using the ethanolic injection method. The anti-ageing activity was assessed by analyzing the epidermal thickness, number of sunburn cells, distance between collagen fibres, and number of fibroblasts. While the histologic specimen scoring was carried out for the in vivo penetration study. The ethosomal formulation had been found to have better penetration ability since it was able to reach the lower dermis area compared to the liposomes, which only reached the upper dermis. The ethosomal formulation of C. heyneana extract exhibited a better anti-ageing activity based on the parameters of epidermal thickness, sunburn cell count, fibroblast count, and the distance between collagen fibres in rat skin histology. Ethosomes have been found to be a more proficient carrier system for transdermal delivery of C. heyneana extract compared to liposomes. Meanwhile, their penetration correlated with the effectivity of the formulation, suggesting that the vesicular system enhanced the penetration ability of the extract.

The characteristics and biological activity enhancements of melatonin encapsulations for skin care product applications

Melatonin (MLT) exhibits antioxidant, ultraviolet protection, anti-inflammatory, and anti-aging properties. However, its effectiveness is limited by instability, a short half-life, and incompatible absorption. In this research, we encapsulated melatonin (MLT) in transfersomes (MT) and niosomes (MN) to enhance their properties and investigate their effects through in vitro cell assays using murine macrophages cells and human foreskin fibroblasts cells. The vesicle morphology, vesicle size, polydispersity index, zeta potential, entrapment efficiency (EE%), attenuated total reflectance-Fourier transform spectroscopy (ATR-FTIR) spectra, along with in vitro release, permeation profiles, and stability study were also evaluated. The results showed that both encapsulations displayed spherical morphology at the nanometric scale, their great physical stability and provided an EE% range of 58–78%. The MLT incorporation into the vesicle was confirmed by the ATR-FTIR spectra. Additionally, the encapsulation’ release profiles fitted with the Higuchi model, indicating controlled release of melatonin. Furthermore, MT showed greater permeability than MN and MS including melatonin deposition. In cell assays, MT exhibited significantly higher nitric oxide inhibition and stimulation of collagen compared to MN and MS. Therefore, MT demonstrated the highest possibility for anti-inflammatory and collagen-stimulating activities that could be applied in pharmaceutical or anti-aging cosmetic products.

High-frequency ultrasound in anti-aging skin therapy monitoring

Over the last few decades, high-frequency ultrasound has found multiple applications in various diagnostic fields. The fast development of this imaging technique opens up new diagnostic paths in dermatology, allergology, cosmetology, and aesthetic medicine. In this paper, being the first in this area, we discuss the usability of HFUS in anti-aging skin therapy assessment. The fully automated algorithm combining high-quality image selection and entry echo layer segmentation steps followed by the dermal parameters estimation enables qualitative and quantitative evaluation of the effectiveness of anti-aging products. Considering the parameters of subcutaneous layers, the proposed framework provides a reliable tool for TCA-peel therapy assessment; however, it can be successfully applied to other skin-condition-related problems. In this randomized controlled clinical trial, forty-six postmenopausal women were randomly assigned to the experimental and control groups. Women were treated four times at one-week intervals and applied skin cream daily between visits. The three month follow-up study enables measurement of the long-term effect of the therapy. According to the results, the TCA-based therapy increased epidermal (entry echo layer) thickness, indicating that the thinning process has slowed down and the skin’s condition has improved. An interesting outcome is the obtained growth in the intensity of the upper dermis in the experimental group, which might suggest a reduced photo-aging effect of TCA-peel and increased water content. The same conclusions connected with the anti-aging effect of TCA-peel can be drawn by observing the parameters describing the contribution of low and medium-intensity pixels in the upper dermis. The decreased share of low-intensity pixels and increased share of medium-intensity pixels in the upper dermis suggest a significant increase in local protein synthesis.

A Cost-Based Analysis of Anti-aging Products Across Four Major United States Retailers.

Background In the field of aesthetic dermatology, there is currently very little data on affordability and cost analysis regarding cosmeceuticals as more demand from patients showing interest in cosmeceutical products to reduce and prevent aging continues to grow.Photoaging, a form of extrinsic aging from sun exposure, can be ameliorated by applying sunscreen and retinol products. Topical ascorbic acid and niacinamide have been shown to target the oxidative stress process that contributes to photoaging.These four products have been identified as the cosmeceutical ingredients with the most evidence-based data on photoaging prevention and treatment. Objective Given the demand for effective skin care,the paucity of data on cost differentiation, and the availability of cosmeceutical products, we analyzed the unit cost offour anti-aging products from major online and physical retailers in the United States. Such a cost comparison may facilitate more economically appropriate recommendations on skin care to consumers. Methods and materials We analyzed sunscreen, topical vitamin C (ascorbic acid), topical vitamin B3 (niacinamide), and topical vitamin A (retinol) products sold by four major United States retailers: Walmart, Ulta, Walgreens, and Amazon. The average cost in dollars per ounce (dollar/oz) was calculated for each product category at each retailer. Statistical analyses were done to determine statistical significance for each product category between retailers as well as between each category of product. Results Between the four retailers, Walmart offered the lowest cost per ounce for every product. In contrast, Amazon offered the highest cost per ounce for every product except for sunscreen. We also found that sunscreen products are less expensive per ounce as compared to retinol, ascorbic acid, and niacinamide products. Conclusion Dermatologists should be knowledgeable of product costs when providing patients with anti-aging product recommendations. Our study provides data on the financial cost by retail location of evidence-based anti-aging cosmeceuticals to better guide physicians in patient consulting and economical resource sharing.

Evaluation of Citrus junos Essential Oil as a Promising Anti-aging Ingredient: In Vitro and Clinical Studies on Skin Health Improvement

Purpose: This study assessed Citrus junos essential oil (CJEO) as an anti-aging agent for improving skin health.Methods: CJEO was extracted from authenticated C. junos fruit peels, and its chemical composition was analyzed using a gas chromatographymass spectrometry (GC-MS). In vitro experiments were conducted on human epidermal keratinocytes (HEKs) and human dermal fibroblasts (HDFs) to evaluate CJEO’s impact on cell viability, anti-inflammatory activity, collagen synthesis, and collagen degradation. The expression of TNF-α, IL-1β, IL-6, NF-κB, MMP-1, MMP-3, COL1A1, and COL3A1 was quantified using RT-qPCR. A dermal patch test was performed on human participants to assess potential skin irritation, and clinical evaluations were conducted on 20 female participants to analyze CJEO’s effects on skin elasticity.Results: CJEO effectively inhibited nitric oxide production and significantly suppressed the expression of inflammatory markers, including TNF-α, IL-1β, IL-6, and NF-κB, as well as collagen degradation markers MMP-1 and MMP-3. Furthermore, CJEO treatment upregulated the expression of genes associated with collagen synthesis, specifically COL1A1 and COL3A1. Notably, significant improvements in skin elasticity were observed in the 20 female participants. The dermal patch test indicated excellent tolerance of the CJEO emulsion without causing irritation.Conclusions: CJEO exhibits promising potential as a bioactive ingredient in anti-aging cosmeceuticals. Its anti-inflammatory properties, ability to stimulate collagen synthesis, suggest efficacy in reducing skin aging and promoting overall skin health. Additionally, the dermal patch test confirms CJEO’s safety, supporting its suitability for use in cosmetic formulations. These findings endorse CJEO as an effective and safe component in anti-aging skincare products.

Bioresources in the Formation of New-Generation Products

The development of new-generation products using the potential of Siberian bioresources is relevant in modern conditions. This process is aimed at protecting the health of people living in a polluted urban environment and the threat of the COVID-19 coronavirus pandemic. The article highlights and describes the characteristic distinctive features of the beneficial properties of the wild flora of Siberia, presents the results of the research conducted by the authors, and provides an assessment of the state and potential of the natural resource base and bioresources of the ecosystem of the Siberian taiga. The study summarizes the current trends of the active part of the population of developed countries towards a healthy lifestyle, indicating an annual increase in demand for natural products. The article presents an analysis of scientific developments and their advantages over previously used methods of protection against infections in an indoor air pool. With the help of laboratory tests, the authors evaluated and justified the feasibility of a new proposed product created using bioresources of wild flora of Siberia which are valuable and useful for humans. It is established that the proposed scientific and technical solutions improve the quality of the products offered, stimulate the consumer to preserve health, and encourage reasonable environmental management. The production and implementation of the developed anti-aging products will contribute to the reduction of consumer race and improve the quality of life of the population of large cities.

Vitamin D3 and the Molecular Pathway of Skin Aging

Many women pay attention to skin aging. Signs of aging such as lines, wrinkles, dry skin, hyperpigmentation, and loss of elasticity affect skin appearance and self-confidence. Age, lifestyle, and particularly UV irradiation stimulate reactive oxygen species (ROS) production. ROS induces the breakdown of collagen through matrix metalloproteinases (MMPs). Collagen and elastin play a pivotal role in skin aging. They maintain skin integrity, strength, and resiliency. Antioxidant and keratolytic agents are often used in anti-aging products, including several vitamins such as vitamin A, B, C and E. To date, several studies have been reported in the literature for the effects of vitamin D on collagen synthesis and MMPs inhibition. This review focuses on identifying and assessing the molecular pathways of vitamin D effects related to skin aging. The literature was collected from Google Scholar, Elsevier, Science Direct, PubMed, and Scopus databases and accessed between January 2019 and May 2022. The literature screening was conducted using keywords like “vitamin D3”, “collagen”, “matrix metalloproteinases”, “skin aging” and related to the study topic were included. The effect of vitamin D3 on MMPs inhibition (particularly on MMP-1, MMP-3, and MMP-9) has been widely published. Several studies have reported that vitamin D increases collagen synthesis and clinically improves skin elasticity. However, there have been controversial results regarding how vitamin D3 affects transforming growth factor-β (TGF-β) correlated with skin elasticity by the molecular pathway. Therefore, it can be concluded that vitamin D3 is a potential alternative agent in improving skin aging.

Anti-aging Effect of Allium pseudojaponicum in UVA-irradiated Human Epidermal Keratinocytes.

This study was conducted to investigate the anti-aging effects of Allium pseudojaponicum extract on normal human epidermal keratinocytes (NHEKs). The effects were examined by analyzing gene expressions related to skin hydration using quantitative real-time polymerase chain reaction (qRT-PCR), hyaluronic acid (HA) production using HA-ELISA, cell viability using a cell viability assay, and a phospho-kinase array. Allium pseudojaponicum extract increased the gene expression levels of AQP3/HAS2 and HA protein production in NHEKs while decreasing the overexpressed mRNA levels of KRT1, 10 and FLG genes, known as differentiated keratinocyte markers in NHEKs. Additionally, A. pseudojaponicum extract reduced the phosphorylation of CHK2 and p53 proteins, which are related to cell cycle or epidermal differentiation. This study demonstrated the anti-aging effects of A. pseudojaponicum extract, which could potentially be used as a functional ingredient for skin hydration and anti-aging products.

Development of Anti-Aging Cream Preparations with Active Substances from Plant Extracts: Physicochemical Review and Potential Applications

Indonesia is known to be rich in biodiversity, including various types of plants that can be used as traditional medicines to treat various skin problems, including acne, skin lightening, and anti-aging. In optimizing efforts to overcome aging caused by free radicals, it is important to develop cosmetic formulations that are effective and comfortable to use. Cosmetics can be in various dosage forms, including gels, creams, powders, ointments, and lotions. It is necessary for the formulation and evaluation of anti-aging cream preparations containing active substances from plant extracts. The method used in this study is comparative research, which involves collecting data from various research journals via the internet. Literature research is conducted online through platforms such as Science Direct, ResearchGate, Google Scholar, and other journal sites. In addition, this study provides information on formulations of anti-aging creams containing active substances from plant extracts based on available scientific evidence in the literature. These findings indicate that the formula used in the development of this cream has the potential to be used in anti-aging products containing active substances from plant extracts. Although the results of this review show success in physicochemical stability, it is important to continue further research to test the effectiveness and safety of using this cream on human skin. More in-depth clinical trials will provide stronger evidence of the benefits and safety of this product. Thus, the development of cream preparations based on the results of this review provides a solid foundation for the development of cosmetic products that combine the natural properties of plants with excellence.

Sex-Dependent Skin Aging and Rejuvenation Strategies

The skin, the largest external organ, serves as the primary defensive barrier against various environmental factors such as ultraviolet exposure, pollution, dietary habits, pathogens, and chemical compounds. Consequently, the skin reflects our age through visible signs of aging, such as wrinkles, age spots, dullness, and sagging. This review explores the gender-related aspects of cutaneous aging and the associated dermatological conditions. It highlights the different manifestations of aging in females and males that become evident after the age of 12, emphasizing the susceptibility to conditions such as seborrheic eczema, acne, and rosacea. Treatment strategies often vary between genders due to these disparities. While men tend to experience accelerated skin aging, most anti-aging products and strategies primarily target females. However, there has been a recent shift in men’s priorities, leading to increased interest in maintaining a youthful appearance and seeking cosmetic treatments. The manuscript covers a comprehensive range of modern dermal anti-aging and rejuvenation procedures, including plastic surgery, bio-revitalization methods, lasers, microneedling, and topical treatments. Additionally, it explores promising natural and synthetic therapeutics for combating age-related skin changes. The focus is on understanding the physiological aspects of gender-related cutaneous structure and aging to guide effective and tailored approaches in dermatological practice.

Research Progress on Skin Aging and Active Ingredients.

With the advancement of living standards in modern society and the emergence of an aging population, an increasing number of people are becoming interested in the topic of aging and anti-aging. An important feature of aging is skin aging, and women are particularly concerned about skin aging. In the field of cosmetics, the market share of anti-aging products is increasing year by year. This article reviews the research and development progress of skin aging and related active compounds both domestically and internationally in recent years. The results show that, in terms of the research on skin aging, the popular theories mainly include free radicals and oxidative stress theory, inflammation theory, photoaging theory, and nonenzymatic glycosyl chemistry theory. In terms of research on the active ingredients with anti-aging activities in the skin, there are numerous reports on related products in clinical studies on human subjects, animal experiments, and experimental studies on cell cultures, with a variety of types. Most of the compounds against skin aging are sourced from natural products and their action mechanisms are mainly related to scavenging oxygen free radicals and enhancing antioxidant defenses. This review provides important references for the future research of skin aging and the development of related products. Although there is a great progress in skin aging including related active ingredients, ideal compounds or products are still lacking and need to be further validated. New mechanisms of skin aging, new active ingredients sourced from natural and artificial products, and new pharmaceutical forms including further clinical validations should be further investigated in the future.