Anthropogenic Load Research Articles

Influence of urban environment factors on morphometric parameters and accumulation of secondary metabolites in Cercis canadensis L. and Cercis siliquastrum ‘Alba’

Consideration of the role of green spaces in urban landscaping is extremely important, as they not only decorate the urban landscape, but also effectively reduce air pollution, regulate the microclimate and play an important role in improving the ecology and quality of life of residents. The purpose of the study was to determine the influence of urban environmental factors on the development and secondary metabolism of plants of the genus Cercis L. Plants growing in Kyiv (Ukraine) under conditions with different air humidity, anthropogenic load, and excess or lack of insolation were selected for the study. Plant growth processes were studied by morphometric parameters of annual shoots of Cercis siliquastrum ‘Alba’ and Cercis canadensis L. growing under different conditions. Morphometric methods, fluorescence microscopy, high-performance thin-layer chromatography and regression analysis were used in the study. The research data show that insufficient sunlight has a negative impact on shoot growth. The specificity of the phenolic profiles that can be used to distinguish between the species Cercis canadensis L. and Cercis siliquastrum ‘Alba’ was established. The principal component analysis confirmed that phenolic profiles depend on habitat conditions and affect plant growth and development. The Gaussian model was used to analyse the growth dynamics of annual shoots during the growing season, which allowed us to describe the specifics of internode formation during the growing season quite accurately. This model allowed us to detect the phenomenon of the last internodes falling off at the end of the growing season, which caused the deviation of the morphometric parameters of metameres from the normal distribution along the time axis. Anatomical studies confirmed that the separation of one or more of the last internodes was due to active corking of the internal tissues of the stem, blocking of the xylem and formation of a separating layer, which led to the fall of the shoot tip. This phenomenon shows signs of plant adaptation to low temperatures in winter. The tops of the shoots often do not have time to lignify and accumulate the required amount of cryoprotectants. With the formation of a separating layer, the available energy resources are redistributed to the lateral buds, which receive the necessary resources to prepare for the winter period and provide new growth in the next growing season. The adaptive potential of the two species of the genus Cercis L. confirms the prospects of their use in urban ecosystems, which can positively affect the conservation of biodiversity in general

АНАЛИЗ КЛИМАТИЧЕСКОГО (ПО ДАННЫМ НАБЛЮДЕНИЙ ЗА 1993-2023 ГГ.) И МИКРОБИОКЛИМАТИЧЕСКОГО ОЗДОРОВИТЕЛЬНОГО ПОТЕНЦИАЛА ГОРОДА-КУРОРТА КИСЛОВОДСК

Modern climate change and variability of the natural environment have a significant impact on the quality of life and health of the population, as well as on the development of the recreational and touristic industry in resort cities. Based on the long-term observations (1993-2023), the changes in the following climatic characteristics have been analyzed: surface air temperature, total precipitation, and current microbioclimatic parameters of the resort city of Kislovodsk. It has been found for the past 30 years that the average annual temperature in Kislovodsk according to the scale of balneological significance can be identified as favorable, and the average annual minimum and maximum temperatures can be characterized as the most favorable. The statistical parameters of the analyzed characteristics and their extremes are presented. The microbioclimatic potential of the central part of the city is characterized by an increased level of natural air ionization and high purity of the surface atmosphere in the areas that do not experience high anthropogenic load.

Anthropogenic load effect on formation of hydrogeological conditions of the Shirvan steppe of the Kura-Aras lowland in Azerbaijan

Relevance. Azerbaijan water and soil resources are limited and subject to technogenic impacts more and more every year. Use of water and demand for water in the republic are growing at a higher rate every year. On the other hand, global climate changes have a serious impact on formation of hydrogeological processes. In such circumstances, it is very important to study the formation situations of hydrogeological conditions under the influence of natural and anthropogenic factors, to use the available water resources efficiently and economically, and to take preventive measures against negative processes. Aim. To study the regularity of formation of the hydrogeological conditions of the Shirvan steppe of the Kura-Araz lowland in the Republic of Azerbaijan under the influence of natural and anthropogenic processes over a long period time. Object. Subsoil waters of the Shirvan steppe of the Kura-Araz Lowland in the Republic of Azerbaijan. Methods. Based on the results of the studies (1977 to 2020) of perennial average level of groundwater and degree of mineralization, the rate of soil salinization, the regime of groundwater and based on the materials collected in this direction, the regime types of groundwater were separated and correlative dependence was found by the method of least squares between regime types and regime-shaping factors – atmospheric sediments, river networks, irrigation water, irrigation canals, drainage, etc. The genetic types of the regime were selected according to the factors creating the regime and the synchronicity of the groundwater level change. Results. Under the influence of natural and anthropogenic factors, the level of groundwater, degree of mineralization, chemical composition, salinity and chemical composition of soils have changed. From 1958 to 2020, due to the irrigation melioration, the groundwater level of the area rose by 4.1 m, due to the infiltration of surface water and removal of mineralized water through drainage, their mineralization rate decreased by 16.2 g/l. Based on the results of the research on changes in the level, degree of mineralization and chemical composition of groundwater, soil salinity and chemical composition, and based on the analysis of archival materials collected in this direction, groundwater regime types were selected and correlative dependence between regime types and regime-creating factors by the method of least squares was defined. Due to the synchronicity of regime-creating factors and groundwater level changes, the genetic types of the regime were selected – climate, hydrological, irrigation, irrigation-watering, drainage, irrigation-watering and the fields of their distribution areas were determined.

Comparative assessment of the change in the species composition of vegetation on the territory of the western field of RSAU-MTAA

The article presents the analysis of changes in the floral composition of the western field of the Ecological Station of the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy for 4 years. The research object is located in the center of the megalopolis, it is subject to high anthropogenic load, as it is surrounded on both sides by a road network, and at the same time it is part of the facies of the Forest Experimental Dacha. The site is characterized by close occurrence of groundwater, high density of upper soil horizons, heterogeneous soil structure and mesorelief, resulting of in flooding. As a result of a significant number of anthropogenic and abiotic factors, it is necessary to analyze the vegetation composition and the species richness of the area in order to assess the dynamics of variability of these conditions and to predict the potential stability of the biological diversity of the area. The changes in the species composition were analyzed by describing the vegetation and evaluating quantitative indicators: frequency of occurrence, abundance of species, degree of their dominance according to the O. Drude scale, as well as the Shannon index and indicators of species richness and saturation.The studies carried out showed a pronounced variability in vegetation cover from 2018 to 2022. During the study period, there was a change in plant families and an increase in species diversity: richness and saturation indices. In 2022, there was an increase in the number of species and an increase in the uniformity of their numbers compared to 2018.

Mosses as bioindicators of air pollution with potentially toxic elements in area with different level of anthropogenic load in Karaganda region, Kazakhstan

Mosses as bioindicators of air pollution with potentially toxic elements in area with different level of anthropogenic load in Karaganda region, Kazakhstan

Modern research on the dynamics of soil microbial communities in soils of Azerbaijan

In soils with minimal anthropogenic load the following were found B.cereus, B.muralis, B. Cibi, B. Simplex, Br.laterosporus, and in soils with high anthropogenic load the following were found B. cibi, B.cereus, P.lautus, B. pseudomycoides, B. niacini, B. mojavensis, B. agaradhaerens, B.arsenicus, B.firmus, B.licheniformis, B.atrophaeus, P. polymyxa, B.acidicola, B.drentensis, Br.laterosporus, B.subtilis. In anthropogenically transformed soils, the number of common bacilli species was reduced, while at each point species were found that were not found at other points. The proportion of microorganisms that utilize natural substrates is sharply reduced - in urban soils the number of aerobic spore-forming bacteria of the order Bacillales decreases several times.

The Relationship of Gametes and Somatic Cells of Male Gonads of Vertebrates: Evolutionary, Age and Seasonal Aspects, Adaptive and Reactive Transformations Under the Action of Destabilizing Factors

The aim of the study is a comparative analysis of the morphofunctional interactions of germ and somatic cells in the organs of the male reproductive system of vertebrates during ontogenesis, under conditions of changes in reproductive activity and under the influence of destabilizing factors. Material and methods. Using light and electron microscopy, immunohistochemistry and morphometry, the testes of representatives of various classes of vertebrates (amphibians, reptiles, birds and mammals) were studied. The collection of material was carried out in environmentally safe ecosystems and in ecosystems with high anthropogenic load in the Orenburg region of Russia and in the Aktobe region of the Republic of Kazakhstan. Results. Based on the material of our own studies of the gonads, as well as on the basis of information from modern domestic and foreign literature, the patterns of relationships between germ cells and somatic cells of their microenvironment in the testes of vertebrates are shown. The features of the structural organization of the germinal and endocrine components of the male gonads are considered. The features of the structural organization of intertitial endocrinocytes (Leydig cells) are shown, and their high resistance to the action of various destabilizing factors compared to spermatogenic epithelium is established. Pubertal generation of endocrinocytes in vertebrate testes during reproductive age is characterized by stability in cell numbers and belongs to cell populations of a stable type. The role and significance of the structures of the blood-testis barrier are shown and the morphological equivalents of changes in the permeability of this barrier are established; it is revealed that in the studied animals, under the influence of negative anthropogenic factors, the integrity of the blood-testis barrier in the testes is violated. The action of destabilizing factors leads to a decrease in the number of developing germ cells in the lumen of the epididymal tubules (up to their complete disappearance in individual individuals), which is probably due to the deterioration of the trophic influences on them from the structures of the epididymis. Conclusion. The principle of organization of the main compartments of the testes, the convoluted seminiferous tubules and the interstitium of the organ, formed during evolution, probably turned out to be evolutionarily advantageous, since it is represented in the testes of all amniotes - reptiles, birds and mammals. In the process of evolutionary development, several types of structural organization of convoluted seminiferous tubules and interstitium were formed in the testes of vertebrates. The need to protect developing germ cells from the effects of immunocytes, which led to the formation of a blood-testis barrier in the testes.

Seasonal dynamics of hydration of shoots of resource species of shrubs in the mediterranean subarid subtropics

An attractive region for tourism - the Southern Coast of Crimea, including the vicinity of the Nikitsky Botanical Garden – is experiencing a high year-round anthropogenic load. This has a negative impact on the conservation and renewal of woody and herbaceous resource species, many of which are ornamental, food and medicinal. As part of the study of the possibilities of renewing the resources of ornamental shrubs and assessing their adaptive potential in the conditions of the Mediterranean subtropics, the article characterizes the water content in buds and shoots based on the methodology for determining the percentage of water per dry mass. The dynamics of the hydration of shoots in winter, spring and autumn is shown. A comparative analysis of the saturation of shoots with water in unstable and stable species at different times of the year is carried out. Species with minimum and maximum water saturation of buds and shoots have been identified. The difference is indicated in the studied species in terms of water content before saturation in natural conditions and after, already in thelaboratory. Attention is drawn to the dependence of species stability on the month of saturation. The characteristic of saturation of plant tissues with water is given when weather conditions change in spring (rainy and dry weather, thaw) when modeling climatic conditions in the laboratory. The species in which the maximum saturation with water occurs both during imitation of rainy weather and during a thaw are highlighted. These studies were conducted for the first time on the described species in the conditions of the Mediterranean subtropics.

Key factors in the successful adaptation of the pest <i>Corythucha ciliata</i> Say in the northern subtropics of the Black Sea coast

Aim. To identify a complex of factors in the successful adaptation of the sycamore lace bug Corythucha ciliata Say for the development of methods for regulating its numbers of and effective biological control measures against this dangerous pest. Material and Methods. The research was carried out by studying scientific publications, analysing the dynamics of climate change in the Lazarevsky district of Sochi for 15 years and studying the reporting data of the Lazarevskaya Experimental Plant Protection Station, Branch of the Federal Research Center of Biological Plant Protection for the period 2008 to 2022. Results. 5 key factors of successful adaptation of Corythucha ciliata to new invasion regions have been identified: the factors of food and climatic adaptation, the factor of interaction with natural enemies, the factor of resistance to entomopathogenic organisms and the factor of adaptation to anthropogenic load. The main conditions and possible vectors of further invasion of sycamore lace in new regions have been determined. Conclusion. The development of effective methods of biological control of the invasive pest Corythucha ciliata Say should be based taking into account the totality of qualitative characteristics of the pest's living conditions. The sycamore lace bug Corythucha ciliata Say effectively builds new trophic connections, without prejudice to its population, adapts not only to new climatic conditions, but also to their changes. Natural entomophages and entomopathogens do not significantly affect the development of the Corythucha ciliata population. The pest has adapted to survival in conditions of high anthropogenic load and successfully uses it to invade new regions.

Polymorphism of xenobiotic biotransformation enzyme genes and their role in early pregnancy loss

Considering the progressive decrease in the number of women of fertile age, as well as children and adolescents, the primary medical and social task at the present stage is to preserve the reproductive health and life of women, preserving every desired pregnancy. The concept of demographic policy of the Russian Federation for the period until 2025 defines the global national goals, i.e. to increase the total fertility rate by 1.5 times, to reduce maternal and infant mortality by at least 2 times, and to improve the reproductive health of women, children and adolescents. Today every fifth desirable pregnancy ends in early loss, and the rate of miscarriage does not tend to decrease and, in fact, increases every year. Carriage of susceptibility or candidate genes can alter the course of biochemical processes in women and cause early pregnancy losses. According to the literature, the cytochrome P-450 gene (CYP1A1, CYP1B1), xenobiotic detoxification genes (GSTT1, GSTM1, GSTP1) and many others are recognized as candidate genes (genetic markers) associated with reproductive losses. Adverse pregnancy outcome is usually multifactorial; in turn, the combination of polymorphic variants of different candidate genes can increase the risk of early losses. A detailed study of the role of candidate genes with clarity and confirmation of the interest of candidate genes in the development of pregnancy loss is undeniable. In this regard, the interest of many researchers in studying the polymorphism of genes of the xenobiotic biotransformation enzyme system as one of the causal factors of pregnancy failure is understandable. The study of this group of genes is of particular relevance in regions with developed industry and high anthropogenic load. The article is devoted to the analysis of the existing data of foreign and domestic literature sources concerning the relationship between polymorphism of xenobiotic biotransformation system genes and reproductive losses.

Comprehensive Hygienic Assessment of Anthropogenic Load on the Territory of the Voronezh Region

Introduction: A comprehensive assessment of adverse human health effects of environmental pollutants found in residential areas has been carried out in the Voronezh Region. Objective: To conduct a comprehensive hygienic assessment of the anthropogenic load on the territory of the Voronezh Region. Materials and methods: We analyzed the results of ambient air, drinking water, and soil quality monitoring collected by the Center for Hygiene and Epidemiology in the Voronezh Region in 2018–2022 for compliance with the maximum permissible concentrations specified in Regulations SanPiN 1.2.3685–21. The complex load was estimated in accordance with method guidelines MR 01-19/17-17. Results: We established that pollution of ambient air in residential areas contributed the most (69.8 %) to the total anthropogenic load in the town Pavlovsk; drinking water – to that in the Semiluksky district (36.5 %), and soil – in the Rossoshansky district (10.5 %). In the city of Voronezh, the level of complex anthropogenic load exceeded both the regional and district averages. The problem of ambient air pollution dominates in all districts; it should be noted, however, that air sampling is carried out only in urban areas and urban-type settlements. In rural areas, effects of agriculture-associated nitrate pollution of drinking water is more pronounced. The impact of soil pollution is more pronounced in urban residential areas. At the same time, the excess of maximum permissible concentrations of soil pollutants were registered neither in rural nor in urban areas over the study period. Conclusion: In Voronezh, the level of complex anthropogenic load exceeds the average values estimated for the region and districts. Ambient air pollution with vehicle and industrial emissions make the greatest contributions to this load both on the territory of Voronezh and in the districts. Nitrate pollution of potable water associated with agriculture is a challenge in rural areas.

АНАЛИЗ СТРУКТУРЫ ПРИРОДНЫХ ЛАНДШАФТОВ ТЕРРИТОРИИ ГОРОДА ХАБАРОВСКА

In the work, the authors examine and analyze the Khabarovsk natural landscapes structure at the level of terrain types. Within the city, based on geological and geomorphological differences, 8 types of terrain, characterized by different anthropogenic loads, have been identified. To assess the structure of natural landscapes and anthropogenic load, a map of urban landscape complexes was compiled on a scale of 1:10 000.

Influence of tillage methods on carbon dioxide emissions in spring wheat crops

The agriculture intensification is associated with an increase in anthropogenic load on arable soils. Tillage leads to disruption of the natural process of soil formation in connection with which the soil biota activity changes and the carbon cycle is disrupted. The purpose of the study is to establish the effects of the tillage method on the carbon dioxide emission in spring wheat crops. The emission of carbon dioxide during the growing season of spring wheat varies significantly depending on the soil processing method and temperature. At the beginning of spring wheat development, the daily emission of carbon dioxide during the dump and subsurface tillage method does not exceed 36.0 and 36.2 CO2 kg/ha using zero technology provides a reduction in production CO2 production to 27.8 kg/ha*day. With an increase in soil temperature by July 24, the daily emission increases on a dump and subsurface background to 105.5 and 106.0 CO2 kg/ha*day, on a zero background to 95.4 CO2 kg/ha*day. In the future, it decreases. The total carbon losses during the dump and subsurface tillage methods are 2829 and 2793 kg/ha, the use of zero tillage technology reduces carbon losses in grain agrocenosis by 18%.

ECOLOGİCAL ZONİNG OF THE NATURAL LANDSCAPES OF THE NORTHEASTERN SLOPE OF THE LESSER CAUCASUS

Purpose. The purpose of the research is to study the ecological zoning of natural landscapes of the northeastern slope of the Lesser Caucasus. Method. During the writing of the article, historical, cartographic, mathematical-statistical, comparative analysis methods were used. The northeastern slope of the Lesser Caucasus was chosen as the research object Result. In the article, as a result of the research conducted for the ecological zoning of the natural landscapes of the North-Eastern slope of the Lesser Caucasus, the changes in the structural and functional characteristics of the natural landscape units of the area and the areas where they spread due to natural and anthropogenic influences were studied, landscape complexes were grouped according to the degree of anthropogenic transformation, new landscape types formed in the place of natural landscapes were investigated. At the same time, the main natural landscapes of the area, tugay forest-meadow-shrub landscapes, semi-desert landscapes, dry-desert landscapes, have changed their borders as a result of anthropogenic effects, and new areas where they spread have been studied. Opinions were discussed about the important role of determining a number of aspects of landscape-ecological zoning diagnostics, in revealing the future development trends of individual landscape units. Taking into account the relevance and modernity of the topic, a number of scientific-theoretical and methodological issues of landscape-ecological zoning for the region were analyzed in the area where the research was conducted. The basis of the work is that settlements and their functional farm areas are characterized by relatively high density in the immediate flat areas, terraced alluvial plains of river valleys, mountain depressions and smooth surface plains and the approximately equal distribution of settlements in the Ganja-Gazakh sloping plain throughout the territory, since the climate, lithological and hydrological conditions in the south-eastern part of the territory have changed sharply, the relatively low water supply in the location of settlements is a social and ecological problem, and therefore the location of settlements in the area along the river banks. Scientific novelity. Based on the ecological zoning of landscapes in the area, it is analyzed that the correct assessment of their ecological potential, the preparation of appropriate zones and belts, the assessment of geoecological potential by dividing into zones taking into account the ecogeographical potential of the natural landscapes of the region and anthropogenic loading, is of great scientific and theoretical importance.

Features of maize entomocomplex formation under anthropogenic load in short-crop rotations in Ukraine

Features of maize entomocomplex formation under anthropogenic load in short-crop rotations in Ukraine

ПЕРЕХОДНЫЙ ПЕРИОД В ПЕРЕСТРОЙКЕ СИСТЕМЫ ВЛАГООБОРОТА ВОДОСБОРОВ (ПО ДАННЫМ ВОДНОБАЛАНСОВОЙ СТАНЦИИ КАМЕННАЯ СТЕПЬ)

The aspects of adaptating the moisture circulation system at the catchments of small watercourses to climate change based on the observational data from the Kamennaya Step' water balance station are considered. It is demonstrated that the transformation of the moisture circulation system results from the changes in the balance between the groundwater recharge and leakage with a decrease in the contribution of cryogenic factors to the river basin water regime formation. It is suggested that the transition period in the moisture circulation restructuring in river basins can be divided into two periods: with predomination of groundwater recharge and with predomination of its leakage. The length of the transformation phase in the steppe zone depending on the cryogenic factor is about 40 years. The final stabilization of the hydrological regime will depend on setting the groundwater balance under the specific hydrogeological conditions and anthropogenic load.

ЭТАПЫ ПЛАНИРОВАНИЯ МЕРОПРИЯТИЙ ИНЖЕНЕРНО-ЭКОЛОГИЧЕСКОЙ ЗАЩИТЫ ВОДНЫХ ОБЪЕКТОВ – ВОДОПРИЕМНИКОВ ДРЕНАЖНЫХ ВОД С ОРОШАЕМЫХ ЗЕМЕЛЬ

Purpose: to develop the main stages of planning the measures of engineering and environmental protection of water bodies – drainage water inlets from irrigated lands. Materials and methods. When developing the stages of planning the measures of engineering and environmental protection of water bodies, recommendations for assessing the environmental and economic efficiency of investment projects for agricultural land reclamation and methods for calculating environmental payments necessary to take into account the effectiveness of environmental protective measures were used. Results. Planning the measures of engineering and environmental protection of a water body can be conditionally divided into four stages. At the first stage, the comprehensive monitoring studies of the water system and agrolandscape in the river catchment basin are carried out. The second stage consists of conducting their comprehensive environmental assessment, including the calculation of such indicators as the combinatorial index of water pollution, the indicator of anthropogenic water body load, the total indicator of soil pollution, bottom deposits, the soil loss rate, the soil formation rate, soil salinization, sodium, magnesium alkalinization, as well as the calculation of cost environmental indicators. At the third stage, a set of measures for engineering and environmental protection of a water body is developed, and at the fourth, a quantitative assessment of the effectiveness of the proposed measures is carried out. Conclusion. Environmental indicators that quantitatively characterize the anthropogenic load on water bodies as a result of the irrigation system operation are proposed. A methodical approach is proposed and the main stages of planning the engineering and environmental protection of water bodies – drainage water inlets from irrigated lands are defined, consisting in a comprehensive assessment of all components of the agro-landscape with the subsequent development of engineering, agrotechnical, agroforestry and agrochemical measures that contribute to the formation of a favorable environmental situation in the river basin.

Floristic diversity of the state natural sanctuary "Bushkovskiy forest" (the Kirov region, Russia)

The paper presents the results of the complex analysis of the flora structure of the state natural sanctuary "Bushkovskiy forest". The research had been made on the area of 9274.8 ha within the period of 2004–2023. Route recognition method was used together with geobotanical description of certain phytocoenoses on temporary sample plots made according to traditional methods. There vascular plant flora contains 270 taxon from 196 genera, 73 families, four classes and three divisio. On the territory of the sanctuary a rare species grows which is registered in the Red Data Book of the Russian Federation – Epipogium aphyllum Sw. The plants are distributed into 13 ecological-coenotic groups. As for the number of species, the floodplain meadow (21.9%), nemoral (17.0%), and boreal (12.6%) groups prevail. Among biological types hemicryptophythes prevail (49.6%); as for the biomorphological structure, dry ground grass polycarpics prevail (65.2%), and, among the latter, short rhizomatous (18.1%) and long rhizomatous (17.4%) grasses prevail. Plants of six hemerobia stages are represented in the flora, for a total, anthropophobic species (species of the a-o-m interval) account for 69.0%. Correlation of hemerobia stages, as well as a small share of adventitious species (3.0%), shows that the natural ecosystem of the sanctuary suffers from a certain anthropogenic load.

Salt Lake as a natural monument terrestrial and aquatic biological diversity

The article provides data on biological diversity of plants, animals, and fungi inhabiting Salt Lake Natural Monument and its surroundings (Tyumen Oblast, Russia). The authors used route and sample plot census methods. The natural monument area is situated within the forest steppe ecotone of the West Siberian Plain. Thus species typical for the adjoining boreal and steppe zones are also registered in the biological communities under study. Aquatic ecosystems of the area include two instances opposed from the species diversity and abundance point of view: the poor one of mineralized Salt Lake and the rich one of fresh water Dolgoye Lake. The total of 166 vascular plant species, 110 vertebrate and 95 invertebrate species, as well as 60 fungi species were registered on the territory. Among these, two plant and eight animal species are listed in different Red Data Books. Recreational use of the territory makes the composition and structure of flora and fauna complexes unique, as the species intolerant to anthropogenic load are depressed, and synanthropes are implanted in the assemblages. Biological diversity transformation accelerates due to an increase of the investigated territory aridity.

Formation and functioning of the water ecosystem of lake China in conditions of increasing anthropogenic load

Formation and functioning of the water ecosystem of lake China in conditions of increasing anthropogenic load