Anthropogenic Environmental Change Research Articles

Genomic prediction of resistance to Hymenoscyphus fraxineus in common ash (Fraxinus excelsior L.) populations.

The increase in introduced insect pests and pathogens due to anthropogenic environmental changes has become a major concern for tree species worldwide. Common ash (Fraxinus excelsior L.) is one of such species facing a significant threat from the invasive fungal pathogen Hymenoscyphus fraxineus. Some studies have indicated that the susceptibility of ash to the pathogen is genetically determined, providing some hope for accelerated breeding programs that are aimed at increasing the resistance of ash populations. To address this challenge, we used a genomic selection strategy to identify potential genetic markers that are associated with resistance to the pathogen causing ash dieback. Through genome-wide association studies (GWAS) of 300 common ash individuals from 30 populations across Poland (ddRAD, dataset A), we identified six significant SNP loci with a p-value ≤1 × 10-4 associated with health status. To further evaluate the effectiveness of GWAS markers in predicting health status, we considered two genomic prediction scenarios. Firstly, we conducted cross-validation on dataset A. Secondly, we trained markers on dataset A and tested them on dataset B, which involved whole-genome sequencing of 20 individuals from two populations. Genomic prediction analysis revealed that the top SNPs identified via GWAS exhibited notably higher prediction accuracies compared to randomly selected SNPs, particularly with a larger number of SNPs. Cross-validation analyses using dataset A showcased high genomic prediction accuracy, predicting tree health status with over 90% accuracy across the top SNP sets ranging from 500 to 10,000 SNPs from the GWAS datasets. However, no significant results emerged for health status when the model trained on dataset A was tested on dataset B. Our findings illuminate potential genetic markers associated with resistance to ash dieback, offering support for future breeding programs in Poland aimed at combating ash dieback and bolstering conservation efforts for this invaluable tree species.

Seasonal species composition, abundance and public health importance of mosquito vectors (Diptera: Culicidae) in Huai Tha Khoei Reservoir, Ratchaburi, Thailand

Abstract. Chaiphongpachara T, Laojuna S. 2024. Seasonal species composition, abundance and public health importance of mosquito vectors (Diptera: Culicidae) in Huai Tha Khoei Reservoir, Ratchaburi, Thailand. Biodiversitas 25: 1264-1275. Mosquitoes are vectors for pathogens that cause life-threatening diseases in humans and animals. Significant mosquito-borne diseases include malaria, dengue, chikungunya, Zika, Japanese encephalitis, and filariasis. Anthropogenic environmental changes can significantly impact the dynamics of disease vector populations, which can lead to outbreaks of mosquito-borne diseases. This study investigated the seasonal variation in species composition and abundance of mosquito vectors around the Huai Tha Khoei Reservoir in Ratchaburi Province, Thailand, across cool, hot, and rainy seasons. A total of 1363 mosquitoes, representing 30 species from five genera, were collected. In particular, 25 of these species, accounting for 83.33% of the total, were identified as vectors of human pathogens. The most dominant species across all seasons was Culex tritaeniorhynchus Giles 1901, representing 39.47% (n=538) of the total, followed by Anopheles barbirostris Wulp 1884 s.l. (14.38%, n = 196) and Culex vishnui Theobald 1901 (9.02%, n=123). The Shannon diversity index was highest during the rainy season (H’= 2.144) and lowest in the hot season (H’=1.788). This is consistent with the results of the UPGMA tree based on beta diversity, indicating that mosquito diversity in the rainy season differs significantly from that in the cool and hot seasons. The findings enhance our understanding of the impact of seasonality on mosquito vectors in this region, which is crucial for developing effective disease control strategies. Additionally, this study serves as a valuable reference for understanding the diversity of mosquito vectors in other areas.

Impact of rapid anthropogenic environmental change on water security in a tropical Andean basin

Rapid anthropogenic environmental change significantly impacts water security (WS) in the Andean highlands, limiting water availability and use. Amidst swift human-induced environmental transformations, discernible trends emerge in increased water demand and heightened ecosystem service valuation. This study examines water usage conflicts in an Andean basin and operationalizes WS using historical analysis for future applications. We focused on WS changes in the Upper Chicamocha River Basin (UCRB), encompassing rural and urban areas. An integrated water security (IWS) framework, with a composite indicator and eight dimensions, is employed to analyze the implications of observed changes over 40 years (1981–2020). Results show a “fair” IWS score 85 % of the time, with 15 % classified as “good”. Evaluation of demand coverage for water usage reveals users benefiting from sufficient water provision (mining, thermoelectric, services, environment, aquaculture, and agriculture), while the industrial and domestic sectors suffer from frequent shortages. Findings emphasize the need for trade-offs in addressing diverse water security outcomes for all users in a changing climate.

The coral microbiome in sickness, in health and in a changing world.

Stony corals, the engines and engineers of reef ecosystems, face unprecedented threats from anthropogenic environmental change. Corals are holobionts that comprise the cnidarian animal host and a diverse community of bacteria, archaea, viruses and eukaryotic microorganisms. Recent research shows that the bacterial microbiome has a pivotal role in coral biology. A healthy bacterial assemblage contributes to nutrient cycling and stress resilience, but pollution, overfishing and climate change can break down these symbiotic relationships, which results in disease, bleaching and, ultimately, coral death. Although progress has been made in characterizing the spatial-temporal diversity of bacteria, we are only beginning to appreciate their functional contribution. In this Review, we summarize the ecological and metabolic interactions between bacteria and other holobiont members, highlight the biotic and abiotic factors influencing the structure of bacterial communities and discuss the impact of climate change on these communities and their coral hosts. We emphasize how microbiome-based interventions can help to decipher key mechanisms underpinning coral health and promote reef resilience. Finally, we explore how recent technological developments may be harnessed to address some of the most pressing challenges in coral microbiology, providing a road map for future research in this field.

Mapping and assessing supply–demand matching of recreational ecosystem services in Shandong province, China

Globally, natural landscapes and their recreational ecosystem services (RES) are both under threat from anthropogenic activities and environmental changes. But the demand for RES increases with the development of human living standards. Therefore, mapping and assessing the supply–demand relationships of RES is crucial for landscape planning, management, and human well-being. This study aimed to study the relationships between supply and demand of RES in Shandong province, China, quantify the supply, demand, and supporting capacity of RES, analyze the degree of matching and coupling between the supply and demand of RES, and explore the effect of terrain gradient to RES. The results indicated that the average value of coupling coordination (CCD) between supply–demand of RES in Shandong province was 0.45, which is a mild balance. According to the supply–demand matching types, areas with low supply-high demand accounted for 6.62%, indicating a serious shortage matching of supply and demand. Using hotel quantity and roads as indicators of the supporting capacity of RES, the overall coordination between landscape aesthetic quality (LAQ) and regional hotel quantity shows a mild imbalance (0.38), while that with the regional road density shows a good balance (0.60). Furthermore, the scenery spots density, RES supply, RES supply–demand CCD and support capacity CCD shows an upward trend with the increase of elevation and slope, an “increased-decreased-increased” trend with the increase of terrain niche index. This study provides spatial matching of RES supply and demand in Shandong province. Optimizing land-use structures and improving natural resource allocation were proposed to boost coordination of RES supply and demand.

Effects of Reduced Seawater pH and Oil Contamination on Bacterial Communities and Biochemical Markers of Estuarine Animal Hosts

Ecosystem functioning depends on complex interactions between microorganisms, hosts, and the environment. Changes in environmental conditions (e.g., ocean acidification) in combination with anthropogenic pollution have been shown to affect the composition and function of free-living microbial communities, but little is known about the effects these stressors on host-associated communities. This study aims to characterize the response of host-associated bacterial communities of the bottom-dwelling polychaete Hediste diversicolor and the epibenthic gastropod Peringia ulvae to oil contamination and reduced seawater pH. The independent and interactive effects of both stressors were simulated under controlled conditions. The response of host-associated bacterial communities was assessed using the high-throughput sequencing of the 16S rRNA gene and several biochemical markers related to host metabolic pathways, e.g., neurotransmission, anaerobic metabolism, biotransformation, oxidative stress, and energy consumption. In H. diversicolor, reduced seawater pH was associated with a high relative abundance of Cyanobacteria, while in P. ulvae oil contamination was associated with a reduction in the relative abundance of Chitinophagales. In P. ulvae, enrichment with oil hydrocarbon-degrading bacteria suggests a possible role of these organisms in the dispersion of oil hydrocarbon degraders. Furthermore, oil supplementation shifted some specific biochemical markers of gastropods related to oxidative stress and energy consumption, which suggests host stress. In general, the bacterial communities and biochemical markers of the gastropod were more affected by stressors than those of the polychaete. Overall, this study contributes to a better understanding of the response of host-associated bacterial communities of benthic macrofauna to anthropogenic contamination and environmental change.

Rushing for "burned" food: Why and how does a group of patas monkeys (Erythrocebus patas) reach freshly burned areas?

Recently, considerable attention has been paid to animal adaptations to anthropogenic environments, such as foraging in burned areas where plants are promoted to regenerate by anthropogenic burning. However, among primates, reports on the utilization of resources that are available immediately after burning have been limited to a few primate species. In this study, we investigated and compared the activity budgets and food categories of a group of patas monkeys (Erythrocebus patas) in freshly burned areas by comparing them with those in previously burned areas and unburned areas. We also assessed the proportion of time spent in the freshly burned area before and after the fire: GPS collars were fitted to five of the six adults in the group, and their patterns when they traveled toward freshly burned and unburned feeding areas were compared. Patas monkeys spent more time in freshly burned areas after the fire, and they visited such areas mostly for feeding, particularly on roasted seeds of Cissus populnea. Furthermore, patas monkeys traveled faster and in a more synchronized way toward freshly burned areas. This "apparent goal-directed" travel began at least 1h before arriving. Results indicate that the group recognized freshly burned areas as valuable, and the monkeys were able to travel in a goal-directed manner to them despite their variable locations. We suggest that smoke from freshly burned areas provides a visual cue with which to orient to the burned areas. Our results also support the notion that some primates are flexible enough to adapt to and benefit from anthropogenic environmental changes.

Impacts of anthropogenic eutrophication on the carbon transfers in lacustrine food webs: A paleolimnological assessment in four mountain lakes

Over the last decades, the intensification of anthropogenic activities and associated disturbances on lake watersheds have led to major changes in lakes trophic functioning through accelerated eutrophication. In many lakes, these changes are characterized by an unprecedented increase in organic carbon fluxes, potentially leading to a shift in biogeochemical cycles and in the balance between carbon sequestration and greenhouse gas emissions. Understanding the response of the carbon cycle to natural and anthropogenic environmental changes is becoming a crucial challenge in the context of increasing global pressures. In this study, we reconstructed the changes in the trophic functioning of the benthic and pelagic food web, in response to accelerated eutrophication in four lakes over the last millenium. Changes in carbon pathways in food webs were assessed using stable carbon isotope analysis of chitinous subfossil remains of Daphnia, Bosmina, and Chironomini archived in sedimentary records. Changes in the trophic state were inferred from sedimentary geochemical analysis, including carbon accumulation rates, Chl a accumulation rates and carbon-to-nitrogen ratios. Agro-pastoral activities were tracked by analysis of coprophilous ascospores. Results provided by this multi-proxy approach highlight recent disruptions in carbon sources and transfer pathways in lakes food webs. In particular, changes in the carbon isotopic signature of pelagic consumers suggest a recent increase in the use of 13C-depleted carbon sources such as methanogenic or respiration-derived carbon linked to recent changes in trophic state under the intensification of anthropogenic pressures.

Temperature and nutrients alter the relative importance of stochastic and deterministic processes in the coastal macroinvertebrates biodiversity assembly on long-time scales.

Macroinvertebrates play a vital role in coastal ecosystems and are an important indicator of ecosystem quality. Both anthropogenic activity and environmental changes may lead to significant changes in the marine macroinvertebrate community. However, the assembly process of benthic biodiversity and its mechanism driven by environmental factors at large scales remains unclear. Here, using the benthic field survey data of 15 years at large spatial and temporal scales from the Yellow Sea Large Marine Ecosystem, we investigated the relative importance of environmental selection, dispersal processes, random-deterministic processes of macroinvertebrates community diversity assembly, and the responses of this relative importance driven by temperature and nutrients. Results showed that the macroinvertebrates community diversity is mainly affected by dispersal. Nitrogen and phosphorus are the most important negative factors among environmental variables, while geographical distance is the main limiting factor of β diversity. Within the range of 0.35-0.70 mg/L of nutrients, increasing nutrient concentration can significantly facilitate the contribution of the decay effect to β diversity. Within the temperature range studied (15.0-18.0°C), both warming and cooling can lead to a greater tendency for species diversity assembly processes to be dominated by deterministic processes. The analysis contributes to a better understanding of the assembly process of the diversity of coastal marine macroinvertebrates communities and how they adapt to global biogeochemical processes.

Sea-ice melt determines seasonal phytoplankton dynamics and delimits the habitat of temperate Atlantic taxa as the Arctic Ocean atlantifies.

The Arctic Ocean is one of the regions where anthropogenic environmental change is progressing most rapidly and drastically. The impact of rising temperatures and decreasing sea ice on Arctic marine microbial communities is yet not well understood. Microbes form the basis of food webs in the Arctic Ocean, providing energy for larger organisms. Previous studies have shown that Atlantic taxa associated with low light are robust to more polar conditions. We compared to which extent sea ice melt influences light-associated phytoplankton dynamics and biodiversity over two years at two mooring locations in the Fram Strait. One mooring is deployed in pure Atlantic water, and the second in the intermittently ice-covered Marginal Ice Zone. Time-series analysis of amplicon sequence variants abundance over a 2-year period, allowed us to identify communities of co-occurring taxa that exhibit similar patterns throughout the annual cycle. We then examined how alterations in environmental conditions affect the prevalence of species. During high abundance periods of diatoms, polar phytoplankton populations dominated, while temperate taxa were weakly represented. Furthermore, we found that polar pelagic and ice-associated taxa, such as Fragilariopsis cylindrus and Melosira arctica, were more common in Atlantic conditions, while temperate taxa, such as Odontella aurita and Proboscia alata, were less abundant under polar conditions. This suggests that sea ice melt may act as a barrier to the northward expansion of temperate phytoplankton, preventing their dominance in regions still strongly influenced by polar conditions. Our findings highlight the complex interactions between sea ice melt, phytoplankton dynamics, and biodiversity in the Arctic.

Nitrogen deposition mediates more stochastic processes in structuring plant community than soil microbial community in the Eurasian steppe.

Anthropogenic environmental changes may affect community assembly through mediating both deterministic (e.g., competitive exclusion and environmental filtering) and stochastic processes (e.g., birth/death and dispersal/colonization). It is traditionally thought that environmental changes have a larger mediation effect on stochastic processes in structuring soil microbial community than aboveground plant community; however, this hypothesis remains largely untested. Here we report an unexpected pattern that nitrogen (N) deposition has a larger mediation effect on stochastic processes in structuring plant community than soil microbial community (those <2 mm in diameter, including archaea, bacteria, fungi, and protists) in the Eurasian steppe. We performed a ten-year nitrogen deposition experiment in a semiarid grassland ecosystem in Inner Mongolia, manipulating nine rates (0-50 g N m-2 per year) at two frequencies (nitrogen added twice or 12 times per year) under two grassland management strategies (fencing or mowing). We separated the compositional variation of plant and soil microbial communities caused by each treatment into the deterministic and stochastic components with a recently-developed method. As nitrogen addition rate increased, the relative importance of stochastic component of plant community first increased and then decreased, while that of soil microbial community first decreased and then increased. On the whole, the relative importance of stochastic component was significantly larger in plant community (0.552±0.035; mean±standard error) than in microbial community (0.427±0.035). Consistently, the proportion of compositional variation explained by the deterministic soil and community indices was smaller for plant community (0.172-0.186) than microbial community (0.240-0.767). Meanwhile, as nitrogen addition rate increased, the linkage between plant and microbial community composition first became weaker and then became stronger. The larger stochasticity in plant community relative to microbial community assembly suggested that more stochastic strategies (e.g., seeds addition) should be adopted to maintain above- than below-ground biodiversity under the pressure of nitrogen deposition.

Same, same, but different: dissimilarities in the hydrothermal germination performance of range-restricted endemics emerge despite microclimatic similarities.

Seed germination responses for most narrow-range endemic species are poorly understood, imperilling their conservation management in the face of warming and drying terrestrial ecosystems. We quantified the realized microclimatic niches and the hydrothermal germination thresholds in four threatened taxa (Tetratheca erubescens, Tetratheca harperi, Tetratheca paynterae subsp. paynterae and Tetratheca aphylla subsp. aphylla) that are restricted to individual Banded Ironstone Formations in Western Australia. While T. aphylla subsp. aphylla largely failed to germinate in our trials, all other species demonstrated extended hydrothermal time accumulation (186-500°CMPadays), cool minimum temperatures (7.8-8.5°C), but broad base water potential thresholds (-2.46 to -5.41MPa) under which germination occurred. These slow germination dynamics are suggestive of cool and wet winter months, where soil moisture is retained to a greater capacity in local microsites where these species occur, rather than the warmer and drier conditions in the surrounding arid environment. Hydrothermal time-to-event modelling showed that each species occupied unique hydrothermal germination niches, which correspond with the microclimatic differences the species are exposed to. Our results provide a baseline understanding for environmental and germination thresholds that govern the recruitment, and ultimately the population structure and persistence, of these short-range endemic plants. In addition, our results can aid future conservation, as well as restoration actions such as translocation to bolster population numbers and to mitigate against losses due to anthropogenic disturbance and global environmental change.

A Warming Southern Gulf of Mexico: Reconstruction of Anthropogenic Environmental Changes From a Siderastrea siderea Coral on the Northern Coast of Cuba

AbstractThe Gulf of Mexico is a vital region for the Atlantic Meridional Overturning Circulation (AMOC), that fuels the exchange of heat between the tropics and the polar regions. A weakening of the AMOC would have dire consequences for the planet. First observations and ocean models show that this process has already started. Very limited knowledge of the components that are part of the AMOC such as the Loop Current (LC) make it difficult to understand its dynamics as well as changes in strength or temperature since the onset of the Industrial Revolution. Currently, there are no continuous in situ sea surface temperature or salinity measurements for the southeastern Gulf of Mexico or reconstruction attempts for this region, showing the necessity for high‐resolution climate archives. A Siderastrea siderea coral core was retrieved from the northwestern Cuban coast and used as a sub‐seasonally resolved sea surface temperature and hydroclimate archive. The approach is based on skeletal δ18O, and trace and minor element contents show an increase in temperature over 160 years since 1845 of 2.6–3.3°C. A possible stagnation of the warming trend set in after the 1980s, indicating a potential weakening of the Loop Current. Impacts in sea surface salinity such as El Niño events in the Pacific region can still be detected in the Gulf of Mexico as decreases in salinity in 1998 from the reconstructed δ18OSW coral record. In situ measurements remain crucial to understand the dynamics in the LC and its influence on the AMOC.

PHTALATES IN SEDIMENTS OF BAIKAL REGION LAKES

Phthalates are one of the most common pollutants, the concentrations of which in the environment in the near future, according to scientists, will only grow. High concentrations of these substances pose a serious threat to water bodies, especially for lake ecosystems, which are characterized by slow water exchange and accumulate pollutants faster, thereby reflecting anthropogenic load and environmental changes. The paper presents for the first time the results of determining the phthalate content in the bottom sediments of lakes Arangatui, Bormashovoe, Dukhovoe, Kotokel, Shchuchye. The total content of 6 phthalates varied from 51 to 2222 mkg/kg of dry weight, while the highest concentrations were noted for central stations. The distribution profile of phthalates in the bottom sediments of lakes is basically quite similar – DBP (average content of 213 mkg/kg) and DEHP (189 mkg/kg) prevailed. It is noted that the accumulation of the studied substances occurs mainly in silty sediments, which is indicated by medium and high positive correlations between phthalates, granulometric composition, the content of organic carbon and heavy metals. The high levels of individual phthalates in the sediments of the studied lakes indicate the need to study in detail the sources and distribution mechanisms in the system of “water-bottom sediments”.

Ecological disturbance reduces genomic diversity across an Alpine whitefish adaptive radiation.

Genomic diversity is associated with the adaptive potential of a population and thereby impacts the extinction risk of a species during environmental change. However, empirical data on genomic diversity of populations before environmental perturbations are rare and hence our understanding of the impact of perturbation on diversity is often limited. We here assess genomic diversity utilising whole-genome resequencing data from all four species of the Lake Constance Alpine whitefish radiation. Our data covers a period of strong but transient anthropogenic environmental change and permits us to track changes in genomic diversity in all species over time. Genomic diversity became strongly reduced during the period of anthropogenic disturbance and has not recovered yet. The decrease in genomic diversity varies between 18% and 30%, depending on the species. Interspecific allele frequency differences of SNPs located in potentially ecologically relevant genes were homogenized over time. This suggests that in addition to the reduction of genome-wide genetic variation, the differentiation that evolved in the process of adaptation to alternative ecologies between species might have been lost during the ecological disturbance. The erosion of substantial amounts of genomic variation within just a few generations in combination with the loss of potentially adaptive genomic differentiation, both of which had evolved over thousands of years, demonstrates the sensitivity of biodiversity in evolutionary young adaptive radiations towards environmental disturbance. Natural history collections, such as the one used for this study, are instrumental in the assessment of genomic consequences of anthropogenic environmental change. Historical samples enable us to document biodiversity loss against the shifting baseline syndrome and advance our understanding of the need for efficient biodiversity conservation on a global scale.

Alleviating competition increases raptor breeding success: A case study of Jackdaws and Lesser Kestrels

Anthropogenic changes in the natural environment have led to the need to find alternative resources to ensure the persistence of biodiversity. In birds, nest sites can be a limiting resource in stenotopic populations. A method of active protection is the building of nest boxes. However, attractive nest sites attract target species and their competitors. From the species conservation point of view, our target species was the Lesser Kestrel (Falco naumanni), whose populations have recovered owing to the establishment of nest box colonies across Europe. However, they have been out-competed by Jackdaws (Corvus monedula) in recent years. Because corvids are intelligent and aggressive, we had to artificially control the competition from Jackdaws to allow Lesser Kestrels to nest. We aimed to discourage Jackdaws from taking over the studied colony and to ensure the continued breeding of Lesser Kestrels by manipulating access to the nest boxes. Data were collected during three breeding seasons in a Lesser Kestrel nest box colony using two manipulations in which we prevented Jackdaw occupation by closing some of the nest boxes and then gradually opening them. We found a significantly lower probability of Lesser Kestrels nesting and lower breeding performance in the control year than in the years in which access to the boxes was manipulated. The field experiment shows the high efficiency of manipulating the accessibility of nest boxes by closing and opening them at the right time in the context of their occupation by the target species and thus influencing their breeding performance.

МІСЦЕ ДОСЛІДЖЕНЬ ІНВАЗІЙНОЇ ФЛОРИ У СТРУКТУРІ СУЧАСНОГО ПРИРОДНИЧО-НАУКОВОГО ЗНАННЯ

This study places a strong emphasis on the role of landscape knowledge in investigating phytoinvasions. It underscores the significance of studying invasive flora within the broader context of researching anthropogenic environmental changes. Phytoinvasions are highlighted as crucial factors in shaping anthropogenic landscapes. This research aligns with the emerging scientific discipline known as invasive geobotany, situated at the intersection of biology, geography, and ecology, particularly in the fields of botany, anthropogenic landscape studies, and plant ecology. In the spectrum of contemporary natural knowledge, invasive geobotany occupies a pivotal position, bridging the realms of biology, geography, and ecology. This study underscores that invasive plants are integral elements of anthropogenic landscapes. It emphasizes that anthropogenic changes in vegetation, exemplified by phytoinvasions, occur within the broader context of human-induced environmental transformation. The interdisciplinary facets of invasive geobotany are delineated, highlighting its diverse connections as typified by O. I. Shabliy. These connections encompass genetic connections, which elucidate the genesis of this field of study, informational links, pointing to the sources of empirical data, connections established based on shared research objectives and methodologies, as well as organizational affiliations related to research support within a shared scientific framework. Specifically, genetic connections link invasive botany with botany and phytocenology. Informational interdisciplinary connections and shared research objectives and methods predominantly connect invasive botany with geography, particularly plant geography, transport geography, and anthropogenic landscape studies. Organizational interdisciplinary connections within invasive geobotany can be established both within the biological and geographical sciences. The wide range of interdisciplinary connections in invasive geobotany, coupled with the rapidly increasing number of scientific publications, indicates its status as a burgeoning field within modern natural science. This suggests the potential for a further increase in research activity in this area, including the emergence of new aspects in the study of phytoinvasions in the near future.

Resource co-limitation of community biomass but not structure of an alpine grassland.

Anthropogenic environmental changes are influencing the structure and function of many ecological communities, but their underlying mechanisms are often poorly understood. We conducted a seven-year field experiment to explore the ecological consequences of nitrogen (N) and phosphorous (P) enrichment in a high-altitude Tibetan alpine grassland. We found that the enrichment of both N and P, but not either alone, increased plant above- and below-ground biomass. By contrast, N, but not P, enrichment reduced species richness, and altered plant phylogenetic diversity and structure. Whereas plant species loss and changes in phylogenetic structure were mainly driven by higher soil manganese levels under N addition, they were mainly driven by increased plant belowground biomass under the addition of both N and P. Our study highlights resource co-limitation of community biomass but not structure of the study grassland, while also identifying soil metal toxicity and belowground competition as important mechanisms driving community changes following nutrient amendment.

Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals.

Studies on host-parasite systems that have experienced distributional shifts, range fragmentation, and population declines in the past can provide information regarding how parasite community richness and genetic diversity will change as a result of anthropogenic environmental changes in the future. Here, we studied how sequential postglacial colonization, shifts in habitat, and reduced host population sizes have influenced species richness and genetic diversity of Corynosoma (Acanthocephala: Polymorphidae) parasites in northern European marine, brackish, and freshwater seal populations. We collected Corynosoma population samples from Arctic, Baltic, Ladoga, and Saimaa ringed seal subspecies and Baltic gray seals, and then applied COI barcoding and triple-enzyme restriction-site associated DNA (3RAD) sequencing to delimit species, clarify their distributions and community structures, and elucidate patterns of intraspecific gene flow and genetic diversity. Our results showed that Corynosoma species diversity reflected host colonization histories and population sizes, with four species being present in the Arctic, three in the Baltic Sea, two in Lake Ladoga, and only one in Lake Saimaa. We found statistically significant population-genetic differentiation within all three Corynosoma species that occur in more than one seal (sub)species. Genetic diversity tended to be high in Corynosoma populations originating from Arctic ringed seals and low in the landlocked populations. Our results indicate that acanthocephalan communities in landlocked seal populations are impoverished with respect to both species and intraspecific genetic diversity. Interestingly, the loss of genetic diversity within Corynosoma species seems to have been less drastic than in their seal hosts, possibly due to their large local effective population sizes resulting from high infection intensities and effective intra-host population mixing. Our study highlights the utility of genomic methods in investigations of community composition and genetic diversity of understudied parasites.

Malacological indicators of anthropogenic and natural environmental changes of the Podhale Basin during the last 2000 years. Studies in the Rogoźnik Stream valley (the Carpathian Mountains, Southern Poland)

The lithological and malacological studies covered sediments forming the low terrace of the Rogoźnik Stream in the northwest part of the Podhale Basin. This terrace is characterised by a uniform structure within a significant part of the valley. Three layers of gravel and four layers of sandy and silty muds were found there. A rich and diversified malacofauna was discovered in fine-grained sediments. Its analysis allowed us to characterise environmental conditions during sediment deposition. The age of the individual components of the sedimentary sequence was determined by radiocarbon dating. A distinct change was found in the upper intervals of the sequence, corresponding to the warm phase of the Medieval Climate Optimum. This period is associated with the robust development of agriculture, and processes related to human activities became the main factor shaping the environment, influencing the course of geological processes, and changing the taxonomical and ecological structure of the fauna and flora assemblages found in this area.