Anthelmintic Activity Of Aqueous Extracts Research Articles

Evaluation of anthelmintic and antioxidant efficacy of green-synthesized copper nanoparticles derived from Erioglossum rubiginosum leaf and seed aqueous extracts

The green synthesis of copper nanoparticles refers to the preparation of nanoparticles using eco-friendly and sustainable processes, often involving plant extracts, microorganisms, or other natural sources. As a sustainable approach with many potential applications, plant extracts could be used for the green synthesis of copper nanoparticles. The green synthesis of copper nanoparticles from plants, such as Erioglossum rubiginosum, involves using leaf and seed extracts to reduce copper ions to form nanoparticles. Primarily, copper nanoparticles are confirmed by visual observation of the aluminum vessel’s inner wall exhibiting a glossy, reddish-brown precipitate. Further, the UV–Vis spectrophotometer is employed to detect the presence of CuNPs. The peak is at 631 nm, which is unique to CuNPs. FTIR data show the presence of a capping agent which stabilizes copper nanoparticles. The peaks at 3,265 cm−1 for the hydroxy group (H-bonded OH stretch) indicate alcohols and phenols; 1610 cm−1 for aromatic ring stretch; and 1,075 cm−1 for aliphatic fluoro compounds (C–F stretch). DPPH reducing power assay is used to verify the antioxidant activity. Both plant extracts and the synthesized CuNPs showed considerable antioxidant activity. The seed and leaf extract of Erioglossum rubiginosum used for the green synthesis of copper nanoparticles served as a capping agent due to the presence of antioxidant phytoconstituents. Then, we used Paramphistomum cervi to evaluate the anthelmintic activity of aqueous extracts of leaves and seeds of Erioglossum rubiginosum. After that, we assessed the anthelmintic activity of the green synthesized copper nanoparticles (CuNPs). We observed that the extracts showed a significant and dose-dependent decrease in the paralysis time and death time of parasites whereas better activity was found from the synthesized CuNPs. At last, we claim that comprehensive investigation on plant extracts and CuNPs could be carried out for further study and might pave the way for development of novel therapeutic agents that show efficacy against diverse parasitic infections.

In vitro anthelmintic activity of aqueous extracts of seven medicinal plants against Haemonchus contortus in goats

In vitro anthelmintic activity of aqueous extracts of seven medicinal plants against Haemonchus contortus in goats

The Brown Alga Bifurcaria bifurcata Presents an Anthelmintic Activity on All Developmental Stages of the Parasitic Nematode Heligmosomoides polygyrus bakeri.

The current control of gastrointestinal (GI) parasitic nematodes mainly relies on the widespread use of anthelmintics, which has inevitably led to resistance. Therefore, there is an urgent need to find new sources of antiparasitic compounds. Macroalgae represent a rich source of active molecules and are widely described as having medicinal properties. In the present study, we investigated the potential anthelmintic activity of aqueous extracts from three species of algae (Bifurcaria bifurcata, Grateloupia turuturu and Osmundea pinnatifida) on the murine parasite Heligmosomoides polygyrus bakeri. Using a set of complementary in vitro tests, including larval development assays, egg hatching tests and nematicidal activity assays on larvae and adults, we report the nematicidal activity of aqueous extracts of B. bifurcata. In addition, aqueous extract fractionation using liquid/liquid partitioning with a solvent of increasing polarity was performed in order to identify the groups of active molecules underlying the anthelmintic activity. Non-polar extracts (heptane, ethyl acetate) demonstrated high anthelmintic potential, highlighting the role of non-polar metabolites such as terpenes. Here, we highlight the strong anthelmintic potential of the brown alga B. bifurcata on a mouse model of GI parasites, thus confirming the strong interest in algae as natural alternatives for the control of parasitic nematodes.

IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF AQUEOUS EXTRACT OF ARDISIA COLORATA ROXB. LEAVES IN ADULT EARTHWORMS

Objective: The study aims to evaluate the anthelmintic activity of aqueous extract of Ardisia colorata Roxb. leaves (AQEAC) using adult earthworms (Pheretima posthuman).
 Methods: The total of 24 adult earthworms were divided into four groups, with six worms in each group (n=6). The anthelmintic activity of AQEAC at two different doses (25 mg/ml and 50 mg/ml) was evaluated by assessing the time of paralysis (min) and time of death (min) of the earthworms. Albendazole (25 mg/ml) was used as standard and 2% gum acacia as control.
 Results: The result showed that AQEAChad significant anthelmintic activity (p<0.001) in a dose-dependent manner but was less potent than the standard drug albendazole.
 Conclusion: AQEAC demonstrated significant anthelmintic activity but was less potent than the standard drug albendazole. However, further studies with higher doses are required to evaluate the dose-dependent activity and to evaluate the exact mechanism responsible for anthelmintic activity.

Comparitive Invitro Antihelmintic Activity of Individual and Combined Leaf Extracts (Syzygium cumini and Achras zapota) Against Pheritima Posthuma

Syzygium cumini and Achras zapota leaves were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of leaves of Syzygium cumini, Achras zapota and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Syzygium cumini, Achras zapota and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.0001).

Invitro Antihelmintic Activity of Individual and Combined Leaf Extracts (Punica granatum and Psidium guajava) Against Pheritima Posthuma

Punica granatum and Psidium guajava leaves were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of leaves of Punica granatum, Psidium guajava and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Punica granatum, Psidium guajava and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.001).

Comparitive Invitro Antihelmintic Activity of Electeria cardamom and Amomum subulatum against Pheritima Posthuma

Elettaria cardamomum and Amomum subulatum fruits were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of fruits of Elettaria cardamomum, Amomum subulatum and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Elettaria cardamomum, Amomum subulatum and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.001). Further studies are suggested to isolate the active principles responsible for the activity.

Evaluation of Anthelmintic Potential of Aqueous Extract of the Stem Bark of Bridelia ferruginea (Benth) Euphorbiaceae

Bridelia ferruginea is a common plant often used to cure gastrointestinal infections and has phytomedicinal potential. Similar to other medicinal plants, it also has antimicrobial as well as antiprotozoal potential. This study was carried out to access the anthelmintic activity of aqueous extract of the stem bark of bridelia ferruginea by using a model worm i.e Pheretima posthuma (earthworm). In this assay, three different concentrations i.e 25, 50 and 100mg/ml of above mentioned extract were tested in bioassay by observing time of paralysis and time of death of worms in minutes. Piperazine citrate was used as a standard reference compound along with normal saline as control in the same concentration. The results indicated that brideliaferruginea crude extract possesed anthelmintic activity in dose dependent manner.

In-vitro Anthelmintic Activity of Aqueous Extract of Leaves of Cleome Rutidosperma DC. (Capparidaceae) Against Haemonchus Contortus

Background: Cleome rutidosperma is annual herb found in different places in Bangladesh. Its leaves used as the treatment for Helminthiasis associated with Haemonchus contortus. This parasite is one of the most pathogenic nematodes and responsible for anemia, edema, and death of cattle’s, mainly during summer in warm, humid climates as like Bangladesh. The aim of the study to investigate the in-vitro anthelmintic activity of aqueous extract C. rutidosperma (AECR) leaves against H. contortus. Methods: We performed phytochemical analysis and in-vitro anthelmintic assay to determine the possible phytochemicals, caused by anthelmintic activity against H. contortus. Results: In-vitro anthelmintic assay, the AECR at the doses of 25; 50 and 100mg/mL significantly (p< 0.01) and (p< 0.001) paralyzed and caused the death of H. contortus in a dose-dependent manner. Reference standard drug Albendazole (15 mg/mL) exerts almost same effect as AECR. No paralyzed and death observed at the control group when treated with 0.9% normal saline. Conclusion: This data confirmed that the aqueous extract C. rutidosperma has the in-vitro anthelmintic activity of against H. contortus. C. rutidosperma may offer an alternative source for the control of gastrointestinal nematodes of cattle’s.

In vitro ovicidal and larvicidal activity of Agave sisalana Perr. (sisal) on gastrointestinal nematodes of goats

This study describes the in vitro anthelmintic activity of aqueous extracts (AE), ethyl acetate extracts (EE), flavonoid fractions (FF) and saponin fractions (SF) obtained from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats. The activity of these extracts was evaluated by performing inhibition of egg hatch (EHA) and larval migration (LMI) assays. The EC50 results of the EHA corresponded to 4.7, 0.1 and 0.05mg/mL for EE, EA and FF, respectively. The SF fraction showed no ovicidal activity. The percent efficacies that were observed for the LMI were 50.3, 33.2 and 64.1% for the AE, EE and SF, respectively. The FF fraction did not show activity against the larvae. The analysis of the FF fraction indicates the presence of a homoisoflavonoid. This report suggests that the A. sisalana has activity in vitro against gastrointestinal nematodes of goats. This effect is likely related to the presence of homoisoflavonoid and saponin compounds, which have different actions for specific stages of nematode development.

Atividade anti-helmíntica do extrato aquoso das folhas de Zanthoxylum rhoifolium Lam. (Rutaceae)

The study evaluated the anthelmintic activity of aqueous extract of Zanthoxylum rhoifolium leaves in two experiments. In vitro test, cultures of goat fecal samples were treated with different concentrations of extract (134.5 to 335.0 mg.mL(-1)). In vivo test was composed of 20 sheep: G1: treated with 0.63 g.kg(-1), during four days; G2: same dose, for eight days; G3: ivermectin (200 microg.kg(-1)) and G4 untreated group. In vitro results showed a reduction of Haemonchus spp, Trichostrongylus spp. and Oesophagostomum spp. larvae greater than 95% in the concentrations between 335.0 and 193.7 mg.mL(-1). Faecal egg counting reduction was 51, 56 and 90% in G1, G2 and G3, respectively, while immature stages and adults ranged from 0 to 91% in G1 and from 26 to 94% in G2. Ivermectin effectiveness was 99% for L4 and L5 of H. contortus and 100% for other nematodes species. Clinical and biochemical parameters have remained in the normality and histophatologic analyses did not show alteration suggesting absence of toxicity. Although the great effectiveness of Z. rhoifolium leaves extract in vitro test, it displayed poor efficiency in vivo regarding gastrointestinal nematodes reduction.

Anthelmintic activity of some sudanese medicinal plants

AbstractThe anthelmintic activity of aqueous extracts (0.25–50 mg/mL) from 14 plant species that represent seven families of the Sudanese flora was examined using the free living rhabditid nematode, Caenorhabditis elegans as a test organism. Extracts of Balanites aegyptiaca and Sesbania sesban were the most effective (LC50, 0.8 and 8.0 mg/mL, respectively). Extracts of Albizzia anthelmintica, Cymbopogen narvatus, Abrus precatorius, Rhyncosia minima, Striga hermonthica and Anogeissus leiocarpa (LC50, 9.5–84.6 mg/mL were less effective in this model test system. Extracts of six plant species, Albizzia malacophylla, Gardenia lutea, Physostigma mesoponticum, Salvadora persica, Xeromphis nilotica and Waltheria indica had no effect upon C. elegans survival.