Anteroposterior Location Research Articles

Blunted prefrontal cortical 18fluorodeoxyglucose positron emission tomography response to meta-chlorophenylpiperazine in impulsive aggression.

Impulsive aggression is a prevalent problem and yet little is known about its neurobiology. Preclinical and human studies suggest that the orbital frontal cortex and anterior cingulate cortex play an inhibitory role in the regulation of aggression. Using positron emission tomography, regional metabolic activity in response to a serotonergic stimulus, meta-chlorophenylpiperazine (m-CPP), was examined in 13 subjects with impulsive aggression and 13 normal controls. The anterior cingulate and medial orbitofrontal regions were hypothesized to respond differentially to m-CPP in patients and controls. In the frontal cortex, regional metabolic glucose response to m-CPP was entered into a group (impulsive aggressive, control) x slice (dorsal, middle, orbital) x position (medial, lateral) x location (anterior, posterior) x hemisphere (right, left) mixed-factorial analysis of variance design. A separate group (impulsive aggressive, controls) x anteroposterior location (Brodmann areas 25, 24, 31, 29) x hemisphere (right, left) analysis of variance was used to examine regional glucose metabolism in the cingulate gyrus. Unlike normal subjects, patients with impulsive aggression did not show activation specifically in the left anteromedial orbital cortex in response to m-CPP. The anterior cingulate, normally activated by m-CPP, was deactivated in patients; in contrast, the posterior cingulate gyrus was activated in patients and deactivated in controls. The decreased activation of inhibitory regions in patients with impulsive aggression in response to a serotonergic stimulus may contribute to their difficulty in modulating aggressive impulses.

Relationship between cranial base structure and maxillofacial components in children aged 3-5 years.

The aim of this study was to clarify the inter-relationships between cranial base angle, the morphological variations of maxillofacial components, and growth of the anterior cranial base length. One hundred and twenty-two Japanese children aged 3-5 years with normal occlusion in the primary dentition were included in this investigation. To analyse the relationship between cranial base structure and maxillofacial components, the subjects were divided into three groups according to facial type (prognathic, orthognathic, or retrognathic) assessed by the magnitude of their NSAr angle (as cranial base angle). These categorized cephalometric profiles suggested that the antero-posterior location of the maxillofacial components corresponded to the NSAr angle. Factor analysis of cephalometric variables showed that the variation of maxillofacial components from the S-N line was strongly related to the cranial base angle. This normal range of morphological variation was distinguished from that of growth by the factor loadings. From these results, theoretical models of cephalometric profiles with normal occlusion were computed by linear regression analysis. Using the models, a standard profile related to an arbitrary cranial base angle was obtained for children with normal occlusion, aged 3-5 years.

Nuclei may anchor at specific locations during nuclear determination in Paramecium caudatum

Summary During the conjugation of Paramecium caudatum, the fates of postzygotic nuclear division products are closely associated with their anteroposterior locations at a critical time. Soon after the third division of the synkaryon, one of the four anterior nuclei becomes a micronucleus and the other three degenerate, while all the posterior ones differentiate into macronuclear anlagen. Recently, we reported that heat shock induced abnormal nuclear determination. To understand the mechanism of this phenomenon, the behaviour of spindles in postzygotic cells was studied using an anti-alpha tubulin antibody. In control cells without the heat shock, the spindle lengthened in a fairly straight axial line at first and then curved at the two poles of the cells, elongating continuously but no longer moving the nuclei. The eight nuclei kept their 4 to 4 anteroposterior localization, though the nuclei themselves were rotated by the attached spindles during the third telophase. In heat-treated or emetine-treated cells, spindles also elongated, but they U-turned or wound in the center region of the cells; as a result, all eight nuclei seemed to be passively moved by the spindles and lost anteroposterior localization. All of these results strongly suggest a nuclear anchoring mechanism that controls the anteroposterior nuclear localization and plays an essential role in normal nuclear determination.

Plastic response of the retrospleniocollicular connection after removal of retinal inputs in neonatal rats. An anterograde tracing study.

The effects of neonatal enucleation on the final adult pattern of retrospleniocollicular connection in the rat was studied using the anterograde tracer biotindextranamine 10,000 (BDA) iontophoretically injected in different anteroposterior locations of the retrosplenial cortex. Retrosplenial afferents are normally distributed in all collicular layers beneath the stratum griseum superficiale (SGS) throughout almost the entire rostrocaudal and lateromedial collicular axes. Neonatal enucleation caused an invasion of lower SGS by abundant retrosplenial afferents, whose distribution remained unaltered in intermediate and deep collicular layers. Axons entering the deafferented SGS showed variable morphologies and arborization patterns. Some of them ran lateromedially close to the SGS-stratum opticum (-SO) limit, giving rise to many collaterals which invaded the lower part of the SGS; whereas others formed narrow terminal arbors, mostly branching in the SO. In the intermediate layers, synaptic profiles were mainly found close to the borders of acetylcholinesterase (AChE) patches in both control and enucleated animals, indicating that neonatal enucleation does not alter the final pattern of retrospleniocollicular afferents to these collicular regions. The results presented here demonstrate that neonatal enucleation leads to the development of an aberrant projection from the retrosplenial cortex to the deafferented superficial layers of the superior colliculus. These results provide new information regarding the reorganization of connections subsequent to neonatal enucleation and suggest that, in enucleated animals, nonvisual multisensorial information could be relayed to central circuits which in intact animals belong to the visual system.

Load-sharing between anterior and posterior elements in a lumbar motion segment implanted with an artificial disc.

A nonlinear three-dimensional finite element model of the osteoligamentous L3-L4 motion segment was used to predict changes in posterior element loads as a function of disc implantation and associated surgical procedures. To evaluate the effects of disc implantation on the biomechanics of the posterior spinal elements (including the facet joints, pedicles, and lamina) and on the vertebral bodies. Although several artificial disc designs have been used clinically, biomechanical data-particularly the change in loads in the posterior elements after disc implantation-are sparse. A previously validated intact finite element model was implanted with a ball-and-cup-type artificial disc model via an anterior approach. The implanted model predictions were compared with in vitro data. To study surgical variables, small and large windows were cut into the anulus, and the implant was placed anteriorly and posteriorly within the disc space. The anterior longitudinal ligament was also restored. Models were subjected to either 800 N axial compression force alone or to a combination of 10 N-m flexion-extension moment and 400 N axial preload. Implanted model predictions were compared with those of the intact model. Facet loads were more sensitive to the anteroposterior location of the artificial disc than to the amount of anulus removed. Under 800 N axial compression, implanted models with an anteriorly placed artificial disc exhibited facet loads 2.5 times greater than loads observed with the intact model, whereas posteriorly implanted models predicted no facet loads in compression. Implanted models with a posteriorly placed disc exhibited greater flexibility than the intact and implanted models with anteriorly placed discs. Restoration of the anterior longitudinal ligament reduced pedicle stresses, facet loads, and extension rotation to nearly intact levels. The models suggest that, by altering placement of the artificial disc in the anteroposterior direction, a surgeon can modulate motion-segment flexuralstiffness and posterior load-sharing, even though the specific disc replacement design has no inherent rotational stiffness.

Corticonuclear projections of the cerebellum preserve both anteroposterior and mediolateral pairing patterns.

The aim of the present study was to establish whether a diverging arrangement of the corticonuclear cerebellar projections exists and, if so, what relation it has with the inferior olivary complex. Iontophoretic injections of a 1 : 1 mixture of tetramethylrhodamine dextran amine and biotinylated dextran amine into the cerebellar cortex orthogradely labelled fibre terminals in the cerebellar nuclei and retrogradely labelled cell bodies in the inferior olivary complex. The injections were into A, B, C2, C3, D1 and D2 bands. These injections showed diverging projections to the cerebellar nuclei, with 'primary projections' directed to the nuclear region previously reported to be specifically connected with the injected band and 'secondary projections' directed to other nuclear regions. Secondary projections from the A, C2 and C3 bands diverged to nuclear regions primarily controlled by cortical bands lateral to those injected. Secondary projections from the D1, and D2 bands diverged to nuclear regions primarily controlled by cortical bands medial to those injected. Moreover, injections distributed along the D1 and D2 bands showed similar sets of nuclear targets, while those distributed along the A, C2 and C3 bands showed two sets of nuclear targets in relation to the anteroposterior location of the injected area within these bands. The cortical areas that projected to the same set of nuclear targets were innervated from single olivary regions, while those that projected to different sets of nuclear targets were innervated from different subsets of single regions of the inferior olive. The results suggest that the olivary bands of the cerebellar cortex project to the cerebellar nuclei with a diverging pattern that is organized in both the mediolateral and the anteroposterior axes.

Taking the guesswork out of mandibular symphyseal distraction osteogenesis

Mandibular symphyseal distraction osteogenesis has recently been introduced as a means of resolving arch length deficiencies in the anterior segment and as a method of reducing large vestibular spaces related to a narrow mandible. Accurately relating the required distraction for a given anterior tooth mass and desired future anteroposterior location of the central incisors has not been possible until recently. The relationship between these 3 controlling factors has been mathematically described by the hyperbolic cosine function and a computer program designed for easy use by the clinician. Two clinical cases illustrate the application of the program. A Web site where the program can be downloaded at no cost is mentioned. (Am J Orthod Dentofacial Orthop 2001;119:121-6)

Head position affects the antero-posterior location of tapping points.

This study assessed how the location of the tapping point is affected by changing the head position. An intra-oral tracing device consisted of a metal tracing plate and a central bearing pin was used in five fully dentate subjects. The tracing plate was fixed to the maxillary part of the device parallel to the occlusal plane. The central bearing pin was fixed to the mandibular part of the device and was located at the midpoint of a line connecting the bilateral first molars. The pin was placed perpendicular to the tracing plate. The appliance was inserted in the mouth and the subject was asked to perform tapping movements with the head Camper and Frankfort plane placed horizontally. The tapping movements (n=30) were made at a 30 mm-range of motion, and the incisal and condylar points were tracked with a 6-degree-of-freedom jaw movement tracking system (TRIMET; Tokyo Shizaisha Co., Japan) .The antero-posterior distribution of 30 tapping points and their mean position from intercuspal position were analyzed in incisor and condylar points between two head positions. Result revealed that the condylar points corresponding to the tapping points converged into a small area when the head was positioned with the Camper plane horizontal. However, in both head positions, condylar points were located anteriorly from the intercuspal position. From the results, it can be concluded that head position has it's effects on the distribution and mean position of tapping points. In addition, it can be suggested that from the distribution of scattered tapping points, the head should be positioned with the Camper plane horizontal to record the tapping points at a convergent area.

Characterization of a novel subset of cardiac cells and their progenitors in the Drosophila embryo.

The Drosophila heart is a simple organ composed of two major cell types: cardioblasts, which form the simple contractile tube of the heart, and pericardial cells, which flank the cardioblasts. A complete understanding of Drosophila heart development requires the identification of all cell types that comprise the heart and the elucidation of the cellular and genetic mechanisms that regulate the development of these cells. Here, we report the identification of a new population of heart cells: the Odd skipped-positive pericardial cells (Odd-pericardial cells). We have used descriptive, lineage tracing and genetic assays to clarify the cellular and genetic mechanisms that control the development of Odd-pericardial cells. Odd skipped marks a population of four pericardial cells per hemisegment that are distinct from previously identified heart cells. We demonstrate that within a hemisegment, Odd-pericardial cells develop from three heart progenitors and that these heart progenitors arise in multiple anteroposterior locations within the dorsal mesoderm. Two of these progenitors divide asymmetrically such that each produces a two-cell mixed-lineage clone of one Odd-pericardial cell and one cardioblast. The third progenitor divides symmetrically to produce two Odd-pericardial cells. All remaining cardioblasts in a hemisegment arise from two cardioblast progenitors each of which produces two cardioblasts. Furthermore, we demonstrate that numb and sanpodo mediate the asymmetric divisions of the two mixed-lineage heart progenitors noted above.

Magnetic resonance imaging after surgical transposition defines the anteroposterior location of the rectus muscle pulleys

Introduction: Connective tissue pulleys serve as the functional origins of the rectus extraocular muscles (EOMs) and constrain the sideslip of the posterior EOM bellies after transposition surgery. Anterior to the pulleys, EOM paths appreciably displace to reach their transposed insertions. The inflection points in the EOM paths from minimal posterior displacement to maximal anterior displacement should define the anteroposterior location of the EOM pulleys after transposition. Methods: Contiguous cross-sectional magnetic resonance images were obtained in planes perpendicular to the long axis of the orbit over its entire anteroposterior extent before and after operation in 6 patients who underwent rectus muscle transposition surgery. Four patients underwent full tendon width transposition of the vertical rectus muscles laterally for lateral rectus palsy. Two of these patients had augmentation of the transposition with sutures that fixated the temporal margins of the transposed muscles posteriorly to the sclera adjacent to the borders of the lateral rectus muscle. One patient underwent full tendon width transposition of the horizontal rectus muscles superiorly for superior rectus palsy. One patient underwent full tendon width transposition of both lateral rectus muscles inferiorly for “A” pattern esotropia. Paths of EOMs were defined relative to the area centroid of the orbit. Pulley locations were inferred from EOM paths. The postoperative change in EOM pulley location was obtained by subtracting the preoperative pulley location from the postoperative pulley location for each image plane. Results: For all patients, the postoperative change in EOM belly location was relatively small posterior to the globe-optic nerve junction. The 2 patients with abducens palsy who underwent placement of posterior augmentation sutures, however, demonstrated a significantly larger displacement of the posterior vertical rectus paths compared with similar patients who did not receive augmentation sutures. For all horizontally transposed vertical rectus muscles and inferiorly transposed lateral rectus muscles, the inflection of the EOM path began 3 mm anterior to the globe-optic nerve junction. For the superiorly transposed medial rectus muscle and lateral rectus muscle, the inflection began 6 mm anterior to the globe-optic nerve junction. Conclusions: The anteroposterior locations of the EOM pulleys can be defined by analysis of EOM displacement after transposition surgery. Augmentation of transpositions by posterior suturing displaces the EOM pulleys substantially more than nonaugmented transpositions. (J AAPOS 1999;3:9-14)

Extensive Divergence and Convergence in the Thalamocortical Projection to Monkey Somatosensory Cortex

This study examined the extent of thalamocortical divergence as a potential determinant of activity-dependent representational plasticity in area 3b of adult monkey somatosensory cortex. Single or paired injections of anterogradely transported tracers, of varying anteroposterior extent, were made horizontally from behind in defined parts of the body representation in the ventral posterior lateral (VPL) and/or ventral posterior medial (VPM) thalamic nuclei, and the distribution and density of labeled thalamocortical terminations were mapped in cortex. Injections of increasing size in any dimension of VPL or VPM resulted in increasing accumulation of labeled terminals within the region of projection, implying extensive convergence of individual axons. Anteroposteriorly elongated injections labeled mediolaterally extended but anteroposteriorly restricted zones in cortex. Dorsoventral placement of an injection in VPL or VPM determined anteroposterior location of labeling in cortex. Dual injections separated mediolaterally or dorsoventrally by approximately 1 mm, and in different parts of the thalamic body or head-face representation gave rise to labeled thalamocortical terminations that overlapped extensively. For injection sites at different anteroposterior levels in VPL or VPM, the area of cortical convergence was related to their extent of anteroposterior coincidence. Labeled terminations arising from injections in immediately adjacent parts of VPL and VPM did not overlap in cortex. The extent of thalamocortical divergence and convergence revealed by these experiments is greater than that predictable from labeling of single axons and is sufficiently great to account for representational plasticity that exceeds the 1.5 mm cortical "distance limit."

Corticopontine terminal fibres form small scale clusters and large scale lamellae in the cat.

We investigated whether terminal fibres in the pontine nuclei are arranged in a lamellar pattern like that demonstrated earlier for pontocerebellar neurones. Following tracer injections in visual and parietal cortices and subsequent computer-based 3-D analysis, we found that labelled corticopontine terminal fibres form numerous sharply delimited aggregates of variable shape. Several of the aggregates are cylindroids (diameter 200-300 microns, length 1-3 mm). The aggregates are confined to a lamellar subspace, the position of which depends on the anteroposterior location of the cortical injections. These findings suggest that the cerebroponto-cerebellar system may be organized according to fairly simple, topographical rules. We discuss the implications of our results in relation to the development of corticopontine topographical organization.

Palatal asymmetry in cleft palate subjects.

In subjects with different types of cleft palate, palatal shape and symmetry were evaluated utilizing the moiré contourography technique. The sample consisted of 95 subjects with cleft palate and 68 controls. The differences between cleft and control subjects in the transverse and anteroposterior location of the highest point of the palate, palatal axis angle, and the palatal index were assessed by analysis of variance. Effects of cleft type, gender, developmental stage of the dentition, missing teeth, and eight skeletal and pharyngeal cephalometric variables on palatal shape and symmetry were assessed using multiple-regression analyses. In comparison with the noncleft individuals, the cleft palate subjects showed parallel but clearly more remarkable asymmetry in palatal shape and position of the first maxillary molars. The anteroposterior location of the deepest point in the palate was more posterior, and the palate was relatively shallower. The severity of the cleft type affected both the anteroposterior and transverse position of the highest point in palatal morphology.

BFGF as a possible morphogen for the anteroposterior axis of the central nervous system in Xenopus.

Vertebrate neural development is initiated during gastrulation by the inductive action of the dorsal mesoderm (Spemann's organizer in amphibians) on neighbouring ectoderm, which eventually gives rise to the central nervous system from forebrain to spinal cord. Here we present evidence that bFGF can mimic the organizer action by inducing Xenopus ectoderm cells in culture to express four position-specific neural markers (XeNK-2, En-2, XIHbox1 and XIHbox6) along the anteroposterior axis. bFGF also induced the expression of a general neural marker NCAM but not the expression of immediate-early mesoderm markers (goosecoid, noggin, Xbra and Xwnt-8), suggesting that bFGF directly neuralized ectoderm cells without forming mesodermal cells. The bFGF dose required to induce the position-specific markers was correlated with the anteroposterior location of their expression in vivo, with lower doses eliciting more anterior markers and higher doses more posterior markers. These data indicate that bFGF or its homologue is a promising candidate for a neural morphogen for anteroposterior patterning in Xenopus. Further, we showed that the ability of ectoderm cells to express the anterior markers in response to bFGF was lost by mid-gastrula, before the organizer mesoderm completely underlies the anterior dorsal ectoderm. Thus, an endogenous FGF-like molecule released from the involuting organizer may initiate the formation of the anteroposterior axis of the central nervous system during the early stages of gastrulation by forming a concentration gradient within the plane of dorsal ectoderm.

Errors in alignment of center of pressure and foot coordinates affect predicted lower extremity torques

The purpose of the study was to quantify the effect of errors in spatial alignment between the center of pressure recorded from a force platform and the coordinates of the foot recorded from film on resultant joint torques in the lower extremity during the stance phase of gait. Two-dimensional kinematic and kinetic data from eight subjects performing walking and running were analyzed using inverse dynamics with the obtained center of pressure values and with ±0.5 and ±1.0 cm shifts in the anteroposterior location of the center of pressure under the support foot. Shifting the center of pressure posteriorly increased the flexor (dorsiflexor) torques at the hip and ankle and decreased the extensor (plantarflexor) torques at these joints. This shift also caused an increase in the extensor and a decrease in the flexor torques at the knee. Shifting the center of pressure anteriorly caused the opposite effects at each joint. The ±0.5 and ±1.0 cm shifts in the location of the center of pressure caused, on average, 7 and 14% changes, respectively, in maximum joint torque and angular impulse values. Relative transition times between flexor and extensor torques were either increased or decreased, on average, by 7 and 13%, respectively for the two conditions over all trials. Based on these results, it is concluded that due to potential errors in the spatial alignment of kinetic and kinematic data, joint torques in the literature on gait should be considered as approximations of the true values.

Periosteal migration in the growing mandible: An animal model

Migration of mandibular periosteum and attached musculature was tracked along the inferior border of the ramus in growing and nongrowing guinea pigs ( Cavia porcellus) over a 6-week period. Particulate metallic growth-tracing implants were placed through the bony mandible and adjacent musculature at two anteroposterior locations and two bony reference markers were placed anteriorly. Quantification from weekly radiographs of growing animals showed marked posterior migration of the periosteum, whereas in nongrowing animals there was negligible periosteum movement. Significantly greater migration occurred in posterior (6.37 ± 0.76 mm) implants relative to the anterior implants (3.45 ± 0.86 mm, p < 0.001). The neutral zone, where little periosteal migration occurs, was calculated to be approximately at the anteroposterior center of the molar tooth row. Analysis of the orientation of the medial pterygoid muscle relative to the mandible showed that muscle fibers on average become more horizontal. Thus, the study found differential anteroposterior migration of the mandibular periosteum in growing animals and correlative changes in orientation of the medial pterygoid muscle. (A M J O RTHOD D ENTOFAC O RTHOP 1995;108:22-9.)

Clinical study of location and reproducibility of three mandibular positions in relation to body posture and muscle function

Clinical studies have confirmed the adequate reproducibility of both centric occlusion and centric relation when used as reference positions during treatment; however, the reproducibility of the neuromuscular position has been found inadequate. This study evaluated the location and reproducibility of these three mandibular positions in relation to body posture, sitting and supine, and bilateral muscle activity before and after the insertion of a flat mandibular positioning device equilibrated to balance the muscle functions, as shown by two electromyography biofeedback instruments. Intraoral recordings were made in 11 young subjects with complete natural dentition. Acrylic resin clutches that supported a screw point in the maxillary arch and painted glass in the mandibular arch were used and positioned not to interfere with the occlusion. The distances of the screw scratch from two of the edges of the painted glass were used to measure the anteroposterior and mediolateral locations with a micrometer. The reproducibility was evaluated by measuring the scratch surface by measuring the weight of the print cutouts made from photographs of the scratches taken with a stereoscope. The location and reproducibility of centric occlusion and centric relation were not affected by body posture. A more precise posterior neuromuscular position was obtained in the supine position. The insertion of a mandibular positioning device did not affect centric occlusion but gave a more precise centric relation. Neuromuscular position became as precise as centric occlusion and was located anteroposteriorly between centric occlusion and centric relation.

Raphe of the posterior neural tube in the chick embryo: its closure and reopening as studied in living embryos with a high definition light microscope.

Chick embryos cultured on a curved substratum show a transient enlargement of the posterior neuropore (PN), mimicking the temporary delay of PN closure as seen in the curly tail (ct) mouse mutant (van Straaten et al. [1993] Development 117:1163-1172). In the present study the PN enlargement in the chick embryo was investigated further with a high definition light microscope (HDmic), allowing high resolution viewing of living embryos in vitro. The temporary PN enlargement appeared due to considerable reopening of the raphe of the posterior neural tube, which was followed by reclosure after several hours. The raphe was subsequently studied in detail. It appeared very irregular, with small zones of apposed, open and fused neural folds. During closure, these raphe features shifted posteriorly. A distinct fusion sequence between surface epithelium and neuroepithelium was not seen. During experimental reopening of the raphe in vitro, small bridges temporarily arose, broke and disappeared quickly; they likely represented the first adhesion sites between the neural folds. More prominent adhesion sites partly detached, resulting in bridging filopodia-like connections; they probably represented the first anteroposterior locations of neural fold fusion. Our observations in the living chick embryo in vitro thus show that the caudal neural tube has an irregular raphe with few adhesion sites, which can be readily reopened. As a result of the irregularity, the PN does not close zipper-like, but button-like by forming multiple closure sites.

The Immediate Effect of the Variation of Anteroposterior Laterotrusive Contacts on the Elevator EMG Activity

A unilateral maxillary splint divided into three occlusal blocks was built for eight healthy young adults. The three blocks allowed the anteroposterior location of the laterotrusive contacts to vary and to distribute the occlusal force over equivalent periodontal surfaces. The ipsilateral masseteric and temporalis electromyogram (EMG) activities were recorded with surface electrodes during maximal voluntary clenching. The elevator EMG activity with the anterior occlusal block in place was significantly lower than with the intermediate or posterior blocks. The reduction for both elevators with the intermediate block in place did not show a significant variation in comparison to the posterior block of the three blocks inserted. The elevator activity with the three occlusal blocks did not differ from that recorded with the posterior block alone. Neurophysiologic and biomechanical explanations are given related to this laterotrusive elevator muscular behavior.

Fundus measurements with indirect ophthalmoscopy. An experimental approach.

We examined the factors influencing the accuracy of indirect ophthalmoscopic estimates of choroidal tumor size by studying indirect ophthalmoscope photographs of solid black domes implanted in the suprachoroidal space of postmortem human eyes. Measurements of the indirect ophthalmoscope photographs showed clinically significant differences in the absolute diameter of the visualized fundus, which varied with differences in condensing lens power and width, anteroposterior location of viewed fundus, and axial length of the experimental eye. Linear regression analysis demonstrated that the field of view of each condensing lens varied similarly with axial length and that the majority of variation in field size for each condensing lens was attributable to axial length variation. Equatorial fields of view averaged 11% smaller than posterior fields of view. The accuracy of estimated absolute intraocular dimensions using indirect ophthalmoscopy potentially approaches +/- 5%.