Antarctic Environment Research Articles

Molecular-level chemical composition of aerosol and its potential source tracking at Antarctic Peninsula

Marine organic aerosols play crucial roles in global climatic systems. However, their chemical properties and relationships with various potential organic sources still need clarification. This study employed high-resolution mass spectrometry to investigate the identity, origin, and transportation of organic aerosols in pristine Antarctic environments (King Sejong Station; 62.2°S, 58.8°W), where complex ocean-cryosphere-atmosphere interactions occur. First, we classified the aerosol samples into three clusters based on their air mass transport history. Next, we investigated the relationship between organic aerosols and their potential sources, including organic matter dissolved in the open ocean, coastal waters, and runoff waters. Cluster 1 (C1), in which the aerosols mainly originated from the open ocean area (i.e., pelagic zone-influenced), exhibited a higher abundance of lipid-like and protein-like organic aerosols than cluster 3 (C3), with ratios 1.8- and 1.6-times higher, respectively. In contrast, C3, characterized by longer air mass retention over sea ice and land areas (i.e., inshore-influenced), had higher lignin- and condensed aromatic structures (CAS)-like organic aerosols by 2.2- and 3.4-times compared to C1. Cluster 2 (C2) has intermediate characteristics between C1 and C3 concerning the chemical properties of the aerosols and air mass travel history. Notably, the chemical properties of the aerosols assigned to C1 are closely related to those of phytoplankton-derived organics enriched in the open ocean. In contrast, those of C3 are comparable to those of terrestrial plant-derived organics enriched in coastal and runoff waters. These findings help evaluate the source-dependent properties of organic aerosols in changing Antarctic environment.

Extreme environmental adaptation mechanisms of Antarctic bryophytes are mainly the activation of antioxidants, secondary metabolites and photosynthetic pathways

The environment in Antarctica is characterized by low temperature, intense UVB and few vegetation types. The Pohlia nutans M211 are bryophytes, which are the primary plants in Antarctica and can thrive well in the Antarctic harsh environment. The transcriptional profiling of Pohlia nutans M211 under low temperature and high UVB conditions was analyzed to explore their polar adaptation mechanism in the extreme Antarctic environment by third-generation sequencing and second-generation sequencing. In comparison to earlier second-generation sequencing techniques, a total of 43,101 non-redundant transcripts and 10,532 lncRNA transcripts were obtained, which were longer and more accurate. The analysis results of GO, KEGG, AS (alternative splicing), and WGCNA (weighted gene co-expression network analysis) of DEGs (differentially expressed genes), combined with the biochemical kits revealed that antioxidant, secondary metabolites pathways and photosynthesis were the key adaptive pathways for Pohlia nutans M211 to the Antarctic extreme environment. Furthermore, the low temperature and strong UVB are closely linked for the first time by the gene HY5 (hlongated hypocotyl 5) to form a protein interaction network through the PPI (protein–protein interaction networks) analysis method. The UVR8 module, photosynthetic module, secondary metabolites synthesis module, and temperature response module were the key components of the PPI network. In conclusion, this study will help to further explore the polar adaptation mechanism of Antarctic plants represented by bryophytes and to enrich the polar gene resources.

Penguin guano trace metals release to Antarctic waters: A kinetic modelling

Penguin guano has been considered as a suitable bioindicator of the exposure to environmental contaminants in Antarctic environment. Although trace metal content values in penguin guano have been widely reported, the kinetics of their mobility in seawater have not been determined. In the present study, we have estimated the release rate of dissolved Cd, Co, Cu, Fe, Mn, Mo, Ni, Pb, V, and Zn from Gentoo (Pygoscelis papua) penguins guano to Antarctic seawater by 120 h laboratory and at external natural conditions of temperature and light experiments. A mathematical model using two metal pools guano (labile and equilibrium) and seawater compartments considering pseudo-first-order kinetics, is proposed in order to interpret and predict the release of trace metals. A good statistical agreement between experimental and modelled concentration values allows us obtention of kinetic parameters and partition coefficients (Kdi). These values allow to estimate releases into seawater from 5400 to 6.3 μg/day·penguin of Cu and V, respectively. More than 50 % of the initial content of all the studied elements are released during the first two hours, reaching 90 % release in the decreasing order of speed Ni ≫ Cu ≈ Mo > Mn > Co > Cd ≈ Pb; periods of up to one hour, Fe, V and Zn reach a maximum release and are then readsorbed. Equilibrium releases >90 % for Mo and Cd, and 55 % - 46 % for Co, Ni, Pb and Mn are obtained; Zn with 5.4 %, V with 1.7 % and Fe with 0.88 % show the lowest values. With an overwhelming growth of estimated population south of 60°S of 259.750 breeding pairs we estimate that the Gentoo penguin population is releasing annually in the Southern Ocean, 716 kg Cu, 188 kg Mn, 113 kg Fe, 102 kg Zn, 17.7 kg Mo, 12.0 kg Ni, 8.70 kg Cd, 4.59 kg Co, 6.27 kg Pb and 0.790 kg V of soluble metals.

Basking in the sun: how mosses photosynthesise and survive in Antarctica.

The Antarctic environment is extremely cold, windy and dry. Ozone depletion has resulted in increasing ultraviolet-B radiation, and increasing greenhouse gases and decreasing stratospheric ozone have altered Antarctica's climate. How do mosses thrive photosynthetically in this harsh environment? Antarctic mosses take advantage of microclimates where the combination of protection from wind, sufficient melt water, nutrients from seabirds and optimal sunlight provides both photosynthetic energy and sufficient warmth for efficient metabolism. The amount of sunlight presents a challenge: more light creates warmer canopies which are optimal for photosynthetic enzymes but can contain excess light energy that could damage the photochemical apparatus. Antarctic mosses thus exhibit strong photoprotective potential in the form of xanthophyll cycle pigments. Conversion to zeaxanthin is high when conditions are most extreme, especially when water content is low. Antarctic mosses also produce UV screening compounds which are maintained in cell walls in some species and appear to protect from DNA damage under elevated UV-B radiation. These plants thus survive in one of the harshest places on Earth by taking advantage of the best real estate to optimise their metabolism. But survival is precarious and it remains to be seen if these strategies will still work as the Antarctic climate changes.

High wax ester and triacylglycerol biosynthesis potential in coastal sediments of Antarctic and Subantarctic environments.

The wax ester (WE) and triacylglycerol (TAG) biosynthetic potential of marine microorganisms is poorly understood at the microbial community level. The goal of this work was to uncover the prevalence and diversity of bacteria with the potential to synthesize these neutral lipids in coastal sediments of two high latitude environments, and to characterize the gene clusters related to this process. Homolog sequences of the key enzyme, the wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (WS/DGAT) were retrieved from 13 metagenomes, including subtidal and intertidal sediments of a Subantarctic environment (Ushuaia Bay, Argentina), and subtidal sediments of an Antarctic environment (Potter Cove, Antarctica). The abundance of WS/DGAT homolog sequences in the sediment metagenomes was 1.23 ± 0.42 times the abundance of 12 single-copy genes encoding ribosomal proteins, higher than in seawater (0.13 ± 0.31 times in 338 metagenomes). Homolog sequences were highly diverse, and were assigned to the Pseudomonadota, Actinomycetota, Bacteroidota and Acidobacteriota phyla. The genomic context of WS/DGAT homologs included sequences related to WE and TAG biosynthesis pathways, as well as to other related pathways such as fatty-acid metabolism, suggesting carbon recycling might drive the flux to neutral lipid synthesis. These results indicate the presence of abundant and taxonomically diverse bacterial populations with the potential to synthesize lipid storage compounds in marine sediments, relating this metabolic process to bacterial survival.

Large and in charge: cortisol levels vary with sex, diet, and body mass in an Antarctic predator, the leopard seal

Evaluating physiological responses in the context of a species’ life history, demographics, and ecology is essential to understanding the health of individuals and populations. Here, we measured the main mammalian glucocorticoid, cortisol, in an elusive Antarctic apex predator, the leopard seal (Hydrurga leptonyx). We also examined intraspecific variation in cortisol based on life history (sex), morphometrics (body mass, body condition), and ecological traits (δ15N, δ13C). To do this, blood samples, life history traits, and morphometric data were collected from 19 individual leopard seals off the Western Antarctic Peninsula. We found that adult leopard seals have remarkably high cortisol concentrations (100.35 ± 16.72 μg/dL), showing the highest circulating cortisol concentration ever reported for a pinniped: 147 μg/dL in an adult male. Leopard seal cortisol concentrations varied with sex, body mass, and diet. Large adult females had significantly lower cortisol (94.49 ± 10.12 μg/dL) than adult males (120.85 ± 6.20 μg/dL). Similarly, leopard seals with higher isotope values (i.e., adult females, δ15N: 11.35 ± 0.69‰) had lower cortisol concentrations than seals with lower isotope values (i.e., adult males, δ15N: 10.14 ± 1.65‰). Furthermore, we compared cortisol concentrations across 26 closely related Arctoid taxa (i.e., mustelids, bears, and pinnipeds) with comparable data. Leopard seals had the highest mean cortisol concentrations that were 1.25 to 50 times higher than other Arctoids. More broadly, Antarctic ice seals (Lobodontini: leopard seal, Ross seal, Weddell seal, crabeater seal) had higher cortisol concentrations compared to other pinnipeds and Arctoid species. Therefore, high cortisol is a characteristic of all lobodontines and may be a specialized adaptation within this Antarctic-dwelling clade. Together, our results highlight exceptionally high cortisol concentrations in leopard seals (and across lobodontines) and reveal high variability in cortisol concentrations among individuals from a single location. This information provides the context for understanding how leopard seal physiology changes with life history, ecology, and morphology and sets the foundation for assessing their physiology in the context of a rapidly changing Antarctic environment.

The Distribution of Surface Soil Moisture over Space and Time in Eastern Taylor Valley, Antarctica

Available soil moisture is thought to be the limiting factor for most ecosystem processes in the cold polar desert of the McMurdo Dry Valleys (MDVs) of Antarctica. Previous studies have shown that microfauna throughout the MDVs are capable of biological activity when sufficient soil moisture is available (~2–10% gravimetric water content), but few studies have attempted to quantify the distribution, abundance, and frequency of soil moisture on scales beyond that of traditional field work or local field investigations. In this study, we present our work to quantify the soil moisture content of soils throughout the Fryxell basin using multispectral satellite remote sensing techniques. Our efforts demonstrate that ecologically relevant abundances of liquid water are common across the landscape throughout the austral summer. On average, the Fryxell basin of Taylor Valley is modeled as containing 1.5 ± 0.5% gravimetric water content (GWC) across its non-fluvial landscape with ~23% of the landscape experiencing an average GWC > 2% throughout the study period, which is the observed limit of soil nematode activity. These results indicate that liquid water in the soils of the MDVs may be more abundant than previously thought, and that the distribution and availability of liquid water is dependent on both soil properties and the distribution of water sources. These results can also help to identify ecological hotspots in the harsh polar Antarctic environment and serve as a baseline for detecting future changes in the soil hydrological regime.

Consequence of Climate Change on Biodiversity and Biome of Antarctica - A Short Review

In specific areas of the Arctic and Antarctic regions, air temperatures are expanding at rates more than two times the international average; additionally, there are other direct human impacts on polar regions. A quick change in climate impacting the polar regions will similarly have huge physical and biological consequences for the last existing parts of the planet since the ice-engulfed Arctic Ocean, the Antarctic landform, and the worldwide eminent Antarctic Circumpolar Current (ACC) serve a basic job in managing the Earth’s current circumstance and ocean systems. Polar biodiversity is presently responding to this rapid climate change and more massive impacts will happen in this era. Intensifying the danger to polar biodiversity is the certainty that numerous polar environments have restricted functional redundancy; in case of the disappearance of a solitary keystone species, they may possibly be exposed to descending impacts and complete biological system rebuilding. This study focussed on the contributing factors of ecological change, the effects of natural change on the Antarctic environment, next to the effects of environmental change on the types of Antarctic biodiversity. The most rapidly changing biological agenda on our planet are arranged in the polar regions. This issue is picked to research the effects of natural change on a particular region’s biodiversity.

Comprehensive insights on environmental adaptation strategies in Antarctic bacteria and biotechnological applications of cold adapted molecules.

Climate change and the induced environmental disturbances is one of the major threats that have a strong impact on bacterial communities in the Antarctic environment. To cope with the persistent extreme environment and inhospitable conditions, psychrophilic bacteria are thriving and displaying striking adaptive characteristics towards severe external factors including freezing temperature, sea ice, high radiation and salinity which indicates their potential in regulating climate change's environmental impacts. The review illustrates the different adaptation strategies of Antarctic microbes to changing climate factors at the structural, physiological and molecular level. Moreover, we discuss the recent developments in "omics" approaches to reveal polar "blackbox" of psychrophiles in order to gain a comprehensive picture of bacterial communities. The psychrophilic bacteria synthesize distinctive cold-adapted enzymes and molecules that have many more industrial applications than mesophilic ones in biotechnological industries. Hence, the review also emphasizes on the biotechnological potential of psychrophilic enzymes in different sectors and suggests the machine learning approach to study cold-adapted bacteria and engineering the industrially important enzymes for sustainable bioeconomy.

Geochemical characterization of scoriaceous and unmelted micrometeorites from the Sør Rondane Mountains, East Antarctica: Links to chondritic parent bodies and the effects of alteration

Micrometeorites originate from the interplanetary dust complex and continuously fall to the Earth’s surface in large amounts. About 10 to 20% of micrometeorites are not melted upon reaching the Earth’s surface, preserving the primary features and characteristics of the parent material. Consequently, unmelted micrometeorites, together with scoriaceous micrometeorites, an intermediate form between cosmic spherules and unmelted micrometeorites, are pivotal in documenting the nature and evolution of interplanetary dust as well as the modifications experienced by micrometeorites during atmospheric entry. Based on their petrographic features, here we identified and characterized 64 scoriaceous and unmelted micrometeorites with diameters varying between 90 and 410 μm from fine-grained sediment sampled in the Sør Rondane Mountains of East Antarctica. Based on their size distribution, the micrometeorites from the Sør Rondane Mountains show a clear distinction between unmelted micrometeorites (<300 μm) and cosmic spherules (>400 μm) and imply an accumulation mechanism or exposure history distinct from other collections (e.g., Transantarctic Mountains). Different exposure windows, weathering processes and environmental factors (e.g., snow cover) could affect the size and composition of preserved particles.A selection of the particles (n = 49) was further characterized for geochemical composition and high-precision oxygen isotope ratios to identify potential parent bodies and document their alteration histories. About 63% of the particles, exhibiting both coarse- and fine-grained textures, derive from carbonaceous chondritic precursors. Two particles (∼4%) display anomalously 16O-poor isotopic compositions similar to that previously observed for (giant) cosmic spherules and unmelted micrometeorites, classified as “group 4” particles. These particles are thought to originate from an unidentified chondritic parent body located in a specific region of the protoplanetary disk or may have been characterized by a distinct alteration history, with recent studies linking them to CY carbonaceous chondrites. Only a single fine-grained particle (∼2%) can be assigned to ordinary chondritic parentage with confidence. The partially hydrated fine-grained matrix suggests this particle might be consistent with a Semarkona-like parent body. Approximately 10% of the studied particles exhibit extensive evidence for secondary terrestrial weathering with formation of (hydr)oxides during residence in the Antarctic environment, preventing detailed parent body identification. Ten particles (∼20%) could not be assigned to a specific parent group due to ambiguous oxygen isotope values. Overall, the parent body statistics from this study agree with those reported for different collections of a similar size fraction. Clear associations between textural groups and parent bodies could not be established. Even though unmelted micrometeorites are generally considered pristine and often do not exhibit any obvious petrographic evidence of terrestrial weathering, the chemical and isotopic data obtained here confirm that alteration can occur at the microscale and any data on unmelted particles from Antarctic subaerial collections should be evaluated with caution.

남극 환경에서 다중 로봇 시스템을 위한 최근접 이웃 알고리즘 기반 효율적 다중 작업 스케줄링

This paper addresses the problem of multi-robot task scheduling in Antarctic environments. It is difficult to operate multiple robots in Antarctic environments due to the icy ground condition and the lack of powers. In our previous work, the multi-robot task scheduling in Antarctic environments has been solved using ant colony optimization. Although it worked successfully, it caused much computation time. This paper proposes an efficient multi-robot task scheduling approach using nearest neighbor algorithm in Antarctic environments. The proposed method was tested in both simulated environments and Antarctic environments. The proposed method showed better performance in terms of computation time and cost than ant colony optimization and genetic algorithm.

Insights into abundance, adaptation and activity of prokaryotes in arctic and Antarctic environments

Insights into abundance, adaptation and activity of prokaryotes in arctic and Antarctic environments

Bioprospecting of the Antarctic Bacillus subtilis strain for potential application in leaching hydrocarbons and trace elements from contaminated environments based on functional and genomic analysis

The production of secondary metabolites including biosurfactants by the Bacillus subtilis ANT_WA51 and the evaluation of its ability to leach metals and petroleum derivatives from the soil, using post-culture medium was investigated. The ANT_WA51 strain isolated from a pristine, harsh Antarctic environment produces the biosurfactants surfactin and fengycin, which reduce the surface tension of molasses-based post-culture medium to 26.6 mN m-1 at a critical micellization concentration (CMC) of 50 mg L-1 and a critical micelle dilution (CMD) of 1:19. The presence of biosurfactants and other secondary metabolites in the post-culture medium contributed to significant removal of xenobiotics from contaminated soils in the batch washing experiment - 70% hydrocarbons and 10–23% metals (Zn, Ni and Cu). The isolate's tolerance to different abiotic stresses, including freezing, freeze-thaw cycles, salinity (up to 10%), the presence of metals - Cr(VI), Pb(II), Mn(II), As(V) (up to 10 mM) and Mo(VI) (above 500 mM) and petroleum hydrocarbons (up to 20.000 mg kg-1) as well as the confirmed metabolic activity of these bacteria in toxic environments in the OxiTop® system indicate that they can be used directly in bioremediation. Comparative genomic analysis of this bacteria revealed a high similarity of its genome to the associated plant strains from America and Europe indicating the wide applicability of plant growth-promoting Bacillus subtilis and that the data can be extrapolated to a wide range of environmental strains. An important aspect of the study was to present the absence of inherent features which would indicate its clear pathogenicity enables its safe use in the environment. Based on the obtained results, we also conclude that the use of post-culture medium, obtained on low-cost byproducts like molasses, for leaching contaminants, especially hydrocarbons, is a promising bioremediation method that can be a replacement for the use of synthetic surfactants and provides a base for further large-scale research but the selection of an appropriate leaching may be dependent on the concentration of contaminants.

Stratospheric ozone depletion in the Antarctic region triggers intense changes in sea salt aerosol geochemistry

Since the early 1980s, the Antarctic environment has served as a natural field laboratory for researchers to investigate the effects of stratospheric ozone depletion, which has resulted in increased surface ultraviolet radiation levels. However, its effective threats still present gaps. We report new pieces of evidence of increased ultraviolet radiation impacting West Antarctica sea salt aerosols. Salt aerosols, particularly in the Southern Ocean Sea, play an important role in the radiative earth balance. To disclose the molecular details of sea salt aerosols, we used a synchrotron-based multi-element microscopic speciation of individual microparticles (Scanning Transmission X-ray Microscopy with Near-Edge X-ray Absorption Fine Structure Spectroscopy combined with Computer-Controlled Scanning Electron Microscopy). Here we identified substantial abundances of chlorine-enriched aerosols in sea salt generated by photolytic products, whereas ice core records revealed increased chlorine depletion from the onset of ozone depletion. Our findings reveal that modern sea salt modification has no Holocene precedent.

“The Only Almost Germ-Free Continent Left”

AbstractThis article examines the role of pandemics and viruses in cultural perceptions of Antarctica over the past century. In the popular imagination, Antarctica has often been framed as a place of purity, refuge, and isolation. In a series of fiction and screen texts from the nineteenth century to the present, viruses feature prominently. The texts fall into two categories: narratives in which Antarctica is the sole source of safety in a pandemic-ravaged world and those in which a virus (or another form of contagion) is discovered within the continent itself and needs to be contained. Viruses in these texts are not only literal but also metaphorical, taking the form of any kind of threatening infection, and as such are linked to texts in which Antarctic purity is discursively connected to racial and gendered exclusivity. Based on this comparison, the article argues that ideas of containment and contagion can have political connotations in an Antarctic context, to the extent that they are applied to particular groups of people in order to position them as “alien” to the Antarctic environment. The authors show that the recent media construction of Antarctica during COVID-19 needs to be understood against this disturbing aspect of the Antarctic imaginary, and also that narratives of Antarctic purity are imaginatively linked to both geopolitical exclusions and the melting of Antarctic ice.

The enormous repetitive Antarctic krill genome reveals environmental adaptations and population insights

Antarctic krill (Euphausia superba) is Earth's most abundant wild animal, and its enormous biomass is vital to the Southern Ocean ecosystem. Here, we report a 48.01-Gb chromosome-level Antarctic krill genome, whose large genome size appears to have resulted from inter-genic transposable element expansions. Our assembly reveals the molecular architecture of the Antarctic krill circadian clock and uncovers expanded gene families associated with molting and energy metabolism, providing insights into adaptations to the cold and highly seasonal Antarctic environment. Population-level genome re-sequencing from four geographical sites around the Antarctic continent reveals no clear population structure but highlights natural selection associated with environmental variables. An apparent drastic reduction in krill population size 10 mya and a subsequent rebound 100 thousand years ago coincides with climate change events. Our findings uncover the genomic basis of Antarctic krill adaptations to the Southern Ocean and provide valuable resources for future Antarctic research.

Exploratory Mapping of Blue Ice Regions in Antarctica Using Very High-Resolution Satellite Remote Sensing Data

Mapping spatiotemporal changes in the distribution of blue ice regions (BIRs) in Antarctica requires repeated, precise, and high-resolution baseline maps of the blue ice extent. This study demonstrated the design and application of a newly-developed semi-automatic method to map BIRs in the Antarctic environment using very high-resolution (VHR) WorldView-2 (WV-2) satellite images. We discussed the potential of VHR satellite data for the mapping of BIRs in the Antarctic environment using a customized normalized-difference blue-ice index (NDBI) method devised using yellow, green, and near-infrared spectral bands of WV-2 data. We compared the viability of the newly developed customized NDBI approach against state-of-the-art target detection (TD), spectral processing (SP) and pixel-wise supervised (PSC) feature extraction (FE) approaches. Four semi-automatic FE approaches (three existing plus one newly developed) consisting of 16 standalone FE methods (12 existing + four customized) were evaluated using an extensive quantitative and comparative assessment for mapping BIRs in the vicinity of Schirmacher Oasis, on the continental Antarctic coastline. The results suggested that the customized NDBI approach gave a superior performance and the highest statistical stability when compared with existing FE techniques. The customized NDBI generally rendered the lowest level of misclassification (average RMSE = 654.48 ± 58.26 m2), followed by TD (average RMSE = 987.81 ± 55.05 m2), SP (average RMSE = 1327.09 ± 127.83 m2) and PSC (average RMSE = 2259.43 ± 115.36 m2) for mapping BIRs. Our results indicated that the use of the customized NDBI approach can greatly improve the semi-automatic mapping of BIRs in the Antarctic environment. This study presents the first refined map of distribution of BIRs around the Schirmacher Oasis. The total area of blue ice in the study area was estimated to be 106.875 km2, approximately 61% of the study area. The WV-2 derived BIR map area presented in this study locally refined the existing BIR map derived using Landsat Enhanced Thematic Mapper Plus (ETM+) and the Moderate Resolution Imaging Spectroradiometer (MODIS)-based mosaic of Antarctica (MOA) dataset by ~31% (~33.40 km2). Finally, we discussed the practical challenges and future directions in mapping BIRs across Antarctica.

Polar Policy in Practice: Tour Guiding in Antarctica

This paper examines the role of the Antarctic tour guide as the front-line actor in interpreting and enforcing polar policy on the continent. Drawing on interviews with expedition team members, it asks where guides turn for up to date information about sites; where guides see themselves positioned within the structures of Antarctic governance; and how they experience their role as mediators between visitors and the Antarctic environment. Foregrounding the experiences of those who call Antarctica a workplace, the paper also highlights the perceived role of IAATO in the tourism ecosystem, and provides recommendations for how industry, policy-makers and guides can work together to create a more explicit connection between policy and practice in the future.

Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4-2, a strain obtained from Antarctica.

According to average nucleotide identity (ANI) analysis of the complete genomes, strain 24S4-2 isolated from Antarctica is considered as a potential novel Arthrobacter species. Arthrobacter sp. 24S4-2 could grow and produce ammonium in nitrate or nitrite or even nitrogen free medium. Strain 24S4-2 was discovered to accumulate nitrate/nitrite and subsequently convert nitrate to nitrite intracellularly when incubated in a nitrate/nitrite medium. In nitrogen-free medium, strain 24S4-2 not only reduced the accumulated nitrite for growth, but also secreted ammonia to the extracellular under aerobic condition, which was thought to be linked to nitrite reductase genes nirB, nirD, and nasA by the transcriptome and RT-qPCR analysis. A membrane-like vesicle structure was detected in the cell of strain 24S4-2 by transmission electron microscopy, which was thought to be the site of intracellular nitrogen supply accumulation and conversion. This spatial and temporal conversion process of nitrogen source helps the strain maintain development in the absence of nitrogen supply or a harsh environment, which is part of its adaption strategy to the Antarctic environment. This process may also play an important ecological role, that other bacteria in the environment would benefit from its extracellular nitrogen source secretion and nitrite consumption characteristics.

Snow-Dependent Biogeochemical Cycling of Polycyclic Aromatic Hydrocarbons at Coastal Antarctica.

The temporal trend of polycyclic aromatic hydrocarbons (PAHs) in coastal waters with highly dynamic sources and sinks is largely unknown, especially for polar regions. Here, we show the concurrent measurements of 73 individual PAHs and environmental data, including the composition of the bacterial community, during three austral summers at coastal Livingston (2015 and 2018) and Deception (2017) islands (Antarctica). The Livingston 2015 campaign was characterized by a larger snow melting input of PAHs and nutrients. The assessment of PAH diagnostic ratios, such as parent to alkyl-PAHs or LMW to HMW PAHs, showed that there was a larger biodegradation during the Livingston 2015 campaign than in the Deception 2017 and Livingston 2018 campaigns. The biogeochemical cycling, including microbial degradation, was thus yearly dependent on snow-derived inputs of matter, including PAHs, consistent with the microbial community significantly different between the different campaigns. The bivariate correlations between bacterial taxa and PAH concentrations showed that a decrease in PAH concentrations was concurrent with the higher abundance of some bacterial taxa, specifically the order Pseudomonadales in the class Gammaproteobacteria, known facultative hydrocarbonoclastic bacteria previously reported in degradation studies of oil spills. The work shows the potential for elucidation of biogeochemical processes by intensive field-derived time series, even in the harsh and highly variable Antarctic environment.